• 한국어류학회지
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• 제27권1호
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• pp.26-32
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• 2015

참붕어 심장의 조직학적 구조를 광학현미경을 이용하여 관찰하였다. 참붕어의 심장은 정맥동, 심방, 심실 및 동맥구가 연속적으로 배열되어 있었다. 정맥동은 조직학적으로 심내막, 심근층, 심외막층으로 구분되었고, 심방과 심실은 심내막, 심내막하층, 심외막하층 및 심외막으로 구분되었으며, 동맥구는 심내막, 심내막하층, 중간층, 심외막하층 및 심외막으로 구분되었다. 정맥동과 심방, 심방과 심실 및 심실과 동맥구 사이에는 판막이 존재하였다. 정맥동은 대부분 아교질로 구성된 외벽을 가지고 있었으며 정맥동과 심방의 연결부위에는 고리 모양의 조직이 분포하였다. 심방은 얇은 심근층에 의해서 형성된 비연속적인 테두리가 소주들에 의해 형성된 해면성 심근을 둘러싸고 있었고, 심외막하층과 소주에는 아교질이 분포하였다. 심실의 심근층은 해면성 심근으로 이루어져 있고, 심외막하층에는 혈관이 분포하였으며, 심실의 심외막하층과 소주에는 아교질이 분포하였다. 동맥구에서 심내막하층에 의해 형성된 이랑은 직경과 길이가 매우 다양했고, 심내막 세포는 볼록한 모양이었으며, 심내막하층의 세포들은 불규칙한 모양이었으나 무리를 지어 분포하지는 않았다. 중간층의 세포들은 배열방향이 다르게 불규칙한 층을 이루었고, 심외막하층에서는 서로 다른 모양의 세포들이 관찰되었다. 동맥구의 심내막하층, 중간층 및 심외막하층에는 아교질과 탄력소가 풍부하였고, 심외막은 한 층의 편평한 세포들로 이루어져 있었다.

• 통합자연과학논문집
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• 제16권1호
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• pp.13-31
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• 2023

The sesarmid crab Muradium tetragonum, inhabiting the mangrove, are considered as a key consumer of litter and thereby play an important role in the detritus food chain and energy flow in the mangrove ecosystem. The present investigation was carried out with objectives to enlighten the reproductive system of Muradium tetragonum through histological and histochemical studies. Histological organization of the testis of M. tetragonum revealed that each testis has a lobular structure consisting of several testicular lobules arranged around the collecting duct. Histology of the deferens of M. tetragonum revealed it to be composed of three-layer of tissues along the entire length:the outer connective tissue, the middle muscular and the inner epithelial layer. Based on the histological architecture these three regions are recognized as proximal vas deferens (PVD), middle vas deferens (MVD) and distal vas deferens (DVD). Histological characteristics of the ovary of M. tetragonum during different phases of ovarian development were studied. Based on the colour changes of the ovary and diameter of the oocytes five stages of ovarian development can be pronounced. Histochemical analysis of the male reproductive tissues of M. tetragonum signifies the secretion of a different biomolecule by specifying their origin in the reproductive tissue and their possible transformation into spermatophores. In the female reproductive tissues, histochemical evaluation envisaged the secretory products during different stages of ovarian development The secretory substances of the spermatheca expound on the significance of its secretion in dehiscing the spermatophore wall and in nourishing as well as protecting the spermatozoa.

• Applied Microscopy
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• 제52권
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• pp.13.1-13.6
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• 2022

Microscopic structure of skin in two amphibious mudskipper fish; Boleophthalmus dussumieri Valenciennes, 1837 (B. dussumeri) and Periophthalmus waltoni Koumans, 1941 (P. waltoni) were investigated in relation to their lifestyle. The general structure of skin is the same among the two species. Epidermis in B. dussumeri was thicker significantly than P. waltoni. The dermal bulges were only well developed in the skin of B. dussumeri. Mucous cells were absent in the epidermis of P. waltoni but present in B. dussumeri. Both B. dussumeri and P. waltoni have well-developed swollen middle cells as a shared epidermal feature. The thickness of the middle cell layer of the epidermis in B. dussumeri was significantly greater than in P. waltoni. Capillaries in the dorsal and ventral parts of the body are more closely distributed to the epidermal surface in P. waltoni than in B. dussumeri. The diffusion distance in the dorsal epidermis of P. waltoni was less than that in the ventral epidermis of B. dussumeri. A comparative examination of the skin of mudskipper species suggests that, due to the more terrestrial lifestyle adopted by P. waltoni, the skin contributes more to respiration.

• 한국패류학회지
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• 제29권2호
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• pp.129-137
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• 2013

본 연구는 구리에 노출된 동죽, Mactra veneriformis의 생존율, 호흡율 및 기관계의 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구와 구리 노출구 3개 (0.025, 0.050 and 0.100 mg/L)였다. 실험 결과 구리는 동죽의 생존율과 호흡률의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인되었다. 구리 0.100 mg/L 노출구에서 3주후 사망률은 100%였으며, 호흡률은 대조구에 비해 75% 감소하였다. 기관계의 조직학적 분석 결과, 외투막 상피층과 결합조직층의 변성, 아가미 점액세포의 증가와 상피세포의 괴사, 발에서는 상피층의 붕괴, 점액세포의 감소 및 혈림프동의 확장과 결합조직층의 변성을 나타냈다. 소화맹낭의 소화선세관에서는 호염기성 세포와 상피세포의 위축 및 농도의존적으로 lipofuscin의 축적이 확인되어 저농도의 구리에 노출된다 할지라도 노출기간이 길어짐에 따라 동죽의 재생산 및 생산량 변동에 영향을 미칠 수 있을 것으로 추정된다.

• 대한치과보철학회지
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• 제46권3호
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• pp.307-319
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• 2008

STATEMENT OF PROBLEM: It is known that an anodic oxidation technique, one of the methods for the implant surface treatment, remarkably increased surface area, enhanced wettability and accelerated the initial bone healing. Purpose: This study was performed to evaluate the wettability of anodized titanium surface which has a nanotubular structure, to assess osseointegration after the placement of implant with nano-size tubes on tibia of rats and to analyze quantitatively transferable rhBMP-2 on each surface. MATERIAL AND METHOD: Four different kinds of surface-treated titanium discs (polished (machined surface) group, micro (blasting surface) group, nano (anodizedmachined surface) group, and nano-micro (anodized-blasting surface) group) were fabricated (n=10). Three different media were chosen to measure the surface contact angles; distilled water, plasma and rhBMP-2 solution. After a single drop (0.025 $m{\ell}$) of solution, the picture was taken with the image camera, and contact angle was measured by using image analysis system. For the test of osseointegration, 2 kinds of anodized surface (anodized-machined surface, anodized-blasting surface) implants having 2.0 mm in diameter and 5.0 mm in length inserted into the tibia of Wistar rats. After 3 weeks, tibia were harvested and the specimens were stained with hematoxylin and eosin for histological analysis. To test the possibility of drug delivery, after soaking sample groups in the concentration of 250 ng/$m{\ell}$l of rhBMP-2 for 48 hours, the excess solution of rhBMP-2 were removed. After that, they were lyophilized for 24 hours, and then the rhBMP-2 on the surface of titanium was resolved for 72 hours in PBS. All the extracted solution was analyzed by ELISA. One-way analysis of variance (ANOVA) was performed on the data. RESULTS: The wettability is improved by anodic oxidation. The best wettability was shown on the nano-micro group, and it was followed by nano group, micro group, and polished group. In the histological findings, all implants showed good healing and the new bone formation were observed along the implant surface. After 3 days, nano-micro group delivered the most amount of rhBMP-2, followed by nano group, micro group, and polished group. CONCLUSION: It indicated that anodic oxidation on blasting surface produce functionally graded nano-micro porous structure and enhance hydrophilicity of the surface and osseointegration. The findings suggest that the nano-micro porous structure could be a useful carrier of osteogenic molecules like rhBMP-2.

• 한국식품영양과학회지
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• 제22권6호
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• pp.763-770
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• 1993

To investigate the quality changes during frozen storage, top shell, Omphalius pfeifferi capenteri, was stored at -18$^{\circ}C$, -$25^{\circ}C$ and -3$0^{\circ}C$ immediately after shelling and water holding capacity, protein composition and histological features were examined with the lapsed period of the storage. During the storage period, amount of free drip was increased with higher frozen temperature and longer frozen period, but with the longer storage period, the lower water holding capacity was observed. The extractability and composition of muscle protein, sarcoplasmic protein and stroma protein were rather stable regardless of frozen temperature and frozen storage period. However, the extractability of myofibrillar protein was decreased with higher frozen temperature and longer frozen storage period. On the changes of muscle tissue structure, following points were observed. 1) In the muscle tissue structure of fresh sample, fine muscle fiber was closely distributed all over the tissue regardless of cross and longitudinal section. 2) In tissue structure under frozen state, it was observed that ice crystals apparently grew with the higher storage temperature. Empty spaces between muscle bundles which wee formed by aggregations of muscle fiber were observed after 3 months storage at -18$^{\circ}C$ . 3) Tissue structure in thawed state was restored satisfactorily after 1 month storage regardless of storage temperature. After 3 months storage at -3$0^{\circ}C$, muscle tissue was well restored, but at -18$^{\circ}C$, empty spaces were apparent due to incomplete restoration.

• 대한소아치과학회지
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• 제7권1호
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• pp.75-83
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• 1980

Rickets is not the deposite of minerals in the skeletal tissue and the retardation of skeletal growth in growing in growing animals. This study was undertaken to investigate the histologic effects of experimental rickets on the dental structure of the albino rats, and to show the relationship between the histological effects and the pulpal disease which induced premature loss of the primary teeth. This study was based on material obtained from 40 white rats that were placed on a rachitogenic diet for a period 1 to 56 days after weaning (at 24 days). In addition, a study was made of 25 litter mates, 24 to 80 days, that were fed a normal diet. The following results were obtained: 1. Enamel formation and calcification showed no significant changes and no hypoplasia. 2. Dentin formation and calcification was retarded and disturbed. In the experimental group, predentin/calcified dentin was remarkablly increased. 3. Newly formed dentin showed interglobular texture (less homogenous calcification) and the predentin was significantly wider and thicker, and there was an irregular wave in the basal portion of the rat's incisors. 4. In cementum, Matrix formed at almost a normal rate but calcification was defective. So cementoid tissue was increasesd. 5. The formation of the alveolar bone was at almost a normal rate but calcification was retarded. The trabecular bone was filled with osteoid tissue and thicker than in normal groups.

• 생약학회지
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• 제2권4호
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• pp.163-172
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• 1971

This paper attempts to observe the histological structure of Korean Arisaema ringens $S_{CHOTT}{'s}$ rhizome, to identify it's constituent and to investigate the pharmacological action with it's alcohol extract. The results are : 1) The inner structure of rhizome on the transverse section is divided into external and internal tissues by the ring of intercellular secretary sac. Raphides of calcium oxalate contained in mucilage cell, collateral vascular bundle, are extremely similar to those contained in Pinellia ternata. 2) The organs of the pistillate Arisaema ringens are larger and more plentiful than those of the staminate Arisaema ringens. The sexual identification is easy in the flowering season. 3) The alkaloid is identified by Meyer reagent as white p.p.t. at pH 2 of sulfuric acid. 4) The saponin is indentified remarkably by means of foaming reaction, Lieberman-Burchard reaction and hemolytic reaction. 5) The effect of alcohol extract on the relaxation of the isolated intestine of the rabbit is remarkably shown at the concentration of $10^{-3}g/ml$. 6) The effect of alcohol extract on the isolated ractus muscle of the frog increases the constructive action of acetylcholine at the concentration of $10^{-3}g/ml$. 7) The effect of alcohol extract on the isolated heart movement of the frog is decreased remarkably at the concentration of $10^{-3}g/ml$. 8) The effect of alcohol extract on the blood pressure of the rabbit is decreased by an interavenous injection of $10^{-3}g/kg$.

• 원예과학기술지
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• 제30권1호
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• pp.79-84
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• 2012

An efficient protocol for cryopreservation of madder hairy root cultures has been developed using droplet-vitrification. In previous study, combining loading solution C4 (35% PVS3) and vitrification solution B5 (80% PVS3) was the most effective method. In this study, we tried three types of vitrification solution, B5, A3 (90% PVS2, on ice), and A5 (70% PVS2, on ice). Combining loading solution C4 and vitrification solution A5 (on ice) showed the best regeneration rate in this study. Histological changes of the cells within the hairy root of madder were also observed in different steps. The cells from the hairy roots of the control treatment were full and intact with different size of vacuoles and obvious cell nucleus having a dark nucleolus. After the stage of preparing for cryopreservation (after preculturing, loading, followed by dehydration solution A5 or B5), intercellular spaces had become distinct, and within cells, the cytoplasms had become denser and week plasmolyses had appeared. The cell plasmolyses were much more apparent and we measured the degree of plasmolysis by calculating, the area of cell/the area of cytoplasm. The value of plasmolysis degree was the highest in the combination of preculture, loading solution C4, and dehydration solution A5, 1.97. Because the highest regeneration rates appeared in the treatment of A5 for 20 min, we could assume that the optimal degree of plasmolysis for cryopreservation might be around 1.97. The changes in cell structure during cryopreservation might be a useful basis for the development of a proper long-term preservation method for madder germplasms.

• 한국식품영양과학회지
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• 제23권3호
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• pp.380-386
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• 1994

흰쥐의 간조직에 미치는 gramoxone 독성과 비타민 C의 완화효과에 대해 조사하였다. Gramoxone처리군에서 간세포의 혼탁종창과 지방변화가 사육기간이 증가함에 따라 현저하고 혼탁종창보다는 지방변화가 더 현저하였으며, 특히 간소엽의 문맥야주변대 간세포에서 지방변화가 심하였다. Kupffer세포의 수도 증가하여 4주 사육군에서 제일 많이 증가하였다. Gramoxone-비타민 C 처리군에서 간세포의 혼탁종창과 지방변화가 현저히 감소하였다. 간세포의 glycogen 함량은 정상대조군에 비해 gramoxone처리군에서 다소 증가하는 경향을 나타내었으며 특히 지방변화가 많이 일어난 간세포에서 glycogen 함량이 더 많았다. Gramoxone-비타민 C 처리군에서 gramoxone처리군에 비해 간세포의 glycogen 함량이 감소하는 경향을 나타내었다.