The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Copper Toxicity on Survival, Respiration and Organ Structure of Mactra veneriformis (Bivalvia: Mactridae)

동죽, Mactra veneriformis의 생존, 호흡 및 기관계 구조에 미치는 구리 (Cu)의 독성

  • Shin, Yun Kyung (Aquaculture Management Division, Aquaculture Research Institute, NFRDI) ;
  • Park, Jung Jun (Aquaculture Management Division, Aquaculture Research Institute, NFRDI) ;
  • Lim, Hyun Sig (Department of Marine and Fisheries Resources, Mokpo National University) ;
  • Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University)
  • 신윤경 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 박정준 (국립수산과학원 전략양식연구소 양식관리과) ;
  • 임현식 (목포대학교 해양수산자원학과) ;
  • 이정식 (전남대학교 수산생명의학과)
  • Received : 2013.06.10
  • Accepted : 2013.06.25
  • Published : 2013.06.30
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Abstract

This study was conducted to find out the changes of survivorship, respiration and organ structure of Mactra veneriformis exposed to copper (Cu). Experimental period was four weeks. Experimental groups were composed of one control condition and three copper exposure conditions (0.025, 0.050 and 0.100 mg/L). The results of the study confirmed that copper induces reduction of survival rate and respiration rate and histopathology of organ structure of the bivalve. In the copper concentration of 0.100 mg/L, mortality was 100% after Cu exposure of 3 weeks. Respiration rate was observed exposure groups lower than control decline by 75%. Histological analysis of organ system illustrated expansion of hemolymph sinus, disappearance of epidermal layer and degeneration of connective tissue layer of the mantle. Also, histological degenerations as epithelial necrosis and hyperplasia of mucous cells are recognized in the gill and it was observed expansion of hemolymph sinus, disruption of epithelial layer, decrease of mucous cell and degeneration of connective tissue layer in the foot. In the digestive diverticulum, it was showed atrophy of basophilic cell and degeneration of epithelial cell in the digestive tubules, and as the concentration of copper increased the accumulation of lipofuscin increased.

본 연구는 구리에 노출된 동죽, Mactra veneriformis의 생존율, 호흡율 및 기관계의 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구와 구리 노출구 3개 (0.025, 0.050 and 0.100 mg/L)였다. 실험 결과 구리는 동죽의 생존율과 호흡률의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인되었다. 구리 0.100 mg/L 노출구에서 3주후 사망률은 100%였으며, 호흡률은 대조구에 비해 75% 감소하였다. 기관계의 조직학적 분석 결과, 외투막 상피층과 결합조직층의 변성, 아가미 점액세포의 증가와 상피세포의 괴사, 발에서는 상피층의 붕괴, 점액세포의 감소 및 혈림프동의 확장과 결합조직층의 변성을 나타냈다. 소화맹낭의 소화선세관에서는 호염기성 세포와 상피세포의 위축 및 농도의존적으로 lipofuscin의 축적이 확인되어 저농도의 구리에 노출된다 할지라도 노출기간이 길어짐에 따라 동죽의 재생산 및 생산량 변동에 영향을 미칠 수 있을 것으로 추정된다.

Keywords

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