• Biomolecules & Therapeutics
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• 제20권1호
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• pp.81-88
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• 2012

Histone deacetylases (HDACs) are enzymes involved in the remodelling of chromatin, and have a key role in the epigenetic regulation of gene expression. Histone deacetylase (HDAC) inhibitors are emerging as an exciting new class of potential anti-cancer agents. In recent years, a number of structurally diverse HDAC inhibitors have been identifi ed and these HDAC inhibitors induce growth arrest, differentiation and/or apoptosis of cancer cells in vitro and in vivo. However, the underlying molecular mechanisms remain unclear. This study aimed at investigating the anti-tumor activity of various HDAC inhibitors, IN-2001, using T47D human breast cancer cells. Moreover, the possible mechanism by which HDAC inhibitors exhibit anti-tumor activity was also explored. In estrogen receptor positive T47D cells, IN-2001, HDAC inhibitor showed anti-proliferative effects in dose-and time-dependent manner. In T47D human breast cancer cells showed anti-tumor activity of IN-2001 and the growth inhibitory effects of IN-2001 were related to the cell cycle arrest and induction of apoptosis. Flow cytometry studies revealed that IN-2001 showed accumulation of cells at $G_2$/M phase. At the same time, IN-2001 treatment time-dependently increased sub-$G_1$ population, representing apoptotic cells. IN-2001-mediated cell cycle arrest was associated with induction of cdk inhibitor expression. In T47D cells, IN-2001 as well as other HDAC inhibitors treatment significantly increased $p21^{WAF1}$ and $p27^{KIP1}$ expression. In addition, thymidylate synthase, an essential enzyme for DNA replication and repair, was down-regulated by IN-2001 and other HDAC inhibitors in the T47D human breast cancer cells. In summary, IN-2001 with a higher potency than other HDAC inhibitors induced growth inhibition, cell cycle arrest, and eventual apoptosis in human breast cancer possibly through modulation of cell cycle and apoptosis regulatory proteins, such as cdk inhibitors, cyclins, and thymidylate synthase.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.427-427
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• 2012

Ferroelectric-gate field effect transistor based memory using a nanowire as a conducting channel offers exceptional advantages over conventional memory devices, like small cell size, low-voltage operation, low power consumption, fast programming/erase speed and non-volatility. We successfully fabricated ferroelectric nonvolatile memory devices using both n-type and p-type Si nanowires coated with organic ferroelectric poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] via a low temperature fabrication process. The devices performance was carefully characterized in terms of their electrical transport, retention time and endurance test. Our p-type Si NW ferroelectric memory devices exhibit excellent memory characteristics with a large modulation in channel conductance between ON and OFF states exceeding $10^5$; long retention time of over $5{\times}10^4$ sec and high endurance of over 105 programming cycles while maintaining ON/OFF ratio higher $10^3$. This result offers a viable way to fabricate a high performance high-density nonvolatile memory device using a low temperature fabrication processing technique, which makes it suitable for flexible electronics.

• Nutrition Research and Practice
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• 제7권6호
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• pp.430-438
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• 2013

Increased oxidative stress in obese diabetes may have causal effects on diabetic complications, including dyslipidemia. Lipopolysccharides (LPS) along with an atherogenic diet have been found to increase oxidative stress and insulin resistance. Cranberry has been recognized as having beneficial effects on diseases related to oxidative stress. Therefore, we employed obese diabetic animals treated with an atherogenic diet and LPS, with the aim of examining the effects of cranberry powder (CP) on diabetic related metabolic conditions, including lipid profiles, serum insulin and glucose, and biomarkers of oxidative stress. Forty C57BL/KsJ-db/db mice were divided into the following five groups: normal diet + saline, atherogenic diet + saline, atherogenic diet + LPS, atherogenic diet + 5% CP + LPS, and atherogenic diet + 10% CP + LPS. Consumption of an atherogenic diet resulted in elevation of serum total cholesterol and atherogenic index (AI) and reduction of high density lipoprotein (HDL)-cholesterol. However, with 10% CP, the increase in mean HDL-cholesterol level was close to that of the group with a normal diet, whereas AI was maintained at a higher level than that of the group with a normal diet. LPS induced elevated serum insulin level was lowered by greater than 60% with CP (P < 0.05), and mean serum glucose level was reduced by approximately 19% with 5% CP (P > 0.05). Mean activity of liver cytosolic glutathione peroxidase was significantly increased by LPS injection, however it was reduced back to the value without LPS when the diet was fortified with 10% CP (P < 0.05). In groups with CP, a reduction in mean levels of serum protein carbonyl tended to occur in a dose dependent manner. Particularly with 10% CP, a reduction of approximately 89% was observed (P > 0.05). Overall results suggest that fortification of the atherogenic diet with CP may have potential health benefits for obese diabetes with high oxidative stress, by modulation of physical conditions, including some biomarkers of oxidative stress.

• Preventive Nutrition and Food Science
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• 제21권3호
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• pp.202-207
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• 2016

The aim of this study was to investigate the association of CD36, a class B scavenger receptor, rs6969989 polymorphism with the serum lipid profiles in Korean women, together with their modulation by oily fish consumption. Subjects were participants from the Korean Genome Epidemiology Study (KoGES), which was initiated in 2001 as a large-scale. A total of 4,210 women aged 39 to 70 were included in this study. Data were collected using self-administered questionnaires, anthropometric measurements, and blood chemical analysis. Dietary intake was analyzed using a semi-quantitative food frequency questionnaire. The minor allele frequency for rs6969989 was found in 12% of this population. Homozygotes minor G allele at the rs6868989 exhibited significantly higher high density lipoprotein cholesterol (HDLC) concentrations (P-trend=0.043) and lower fasting glucose (P-trend=0.013) than major allele A carriers. The risk of low HDL-C was significantly lower in homozygotes for the G allele than the A allele carriers (P-trend=0.032). Gene-diet interaction effects between rs6969989 and oily fish intake were significantly associated with the risk of dyslipidemia (P-interaction=0.004). Subjects with homozygotes minor G allele and high oily fish intake generally had a lower risk of dyslipidemia than did those with major allele homozygotes and low oily fish intake. These findings supported that oily fish consumption may modulate the contributions of CD36 rs6969989 on genetic predisposition to the risk of dyslipidemia.

• 한국전자파학회논문지
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• 제15권6호
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• pp.619-626
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• 2004

채널 간격이 100 ㎓로 일정하고 채널 비트율이 40 Gbps인 16-채널 WDM 시스템에 MSSI(Mid-Span Spectral Inversion) 기법을 적용한 경우의 4-광파 혼합(FWM: Four-Wave Mixing)에 의해 왜곡된 채널 신호의 보상 특성을 살펴보았다. MSSl를 통한 광대역 보상을 위해 광 위상 공액기(OPC: Optical Phase Conjugator)의 비선형 매질로 HNL-DSF(Highly-Nonlinear Dispersion Shifted Fiber)를 사용하였다. 우선 MSSI 기법을 이용하면 FWM에 의한 채널 간섭의 영향을 모든 채널에 걸쳐 특정 입력 전력 범위 내에서 비슷한 특성으로 줄일 수 있다는 것을 확인할 수 있었다. 그러나 OPC에 의해 생성되는 공액파 전력이 입력 신호파 전력에 비해 작은 장파장 채널들은 광섬유의 분산 계수가 커질수록 보상의 정도가 줄어드는 것도 알 수 있었다. 즉 분산 계수가 비교적 적은 광섬유를 통해 채널 간격이 일정한 WDM 채널들을 특정 전력 이내에서 양호한 품질로 전송하는데 HNL-DSF를 이용한 MSSI 보상 기법이 효과적이라는 것을 확인할 수 있었다.

• 디지털융복합연구
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• 제12권11호
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• pp.407-414
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• 2014

본 연구의 목적은 컴퓨터 방사선영상에서 X-선 초점 크기와 디지털영상후처리에 따른 공간분해능을 정량적으로 분석하고자 하였다. 초점의 크기는 소초점(0.6 mm)와 대초점(1.2 mm)을 이용하였다. 공간분해능의 정량적 분석은 엣지 측정법의 변조전달함수(MTF)를 이용하였다. 디지털영상후처리는 다단계 이미지 대비 증폭 알고리즘을 이용하여 경계면 증강과 대조도 증강에 따른 50%와 10%의 MTF를 평가하였다. 그 결과 모든 초점에서 MTF 50%의 공간분해능이 대조도 증강보다 경계면 증강에서 유의하게 높았다. 또한 대초점에서 획득된 영상은 디지털영상처리를 통해 공간분해능이 향상되었다. 결론적으로 이러한 결과는 컴퓨터 방사선영상에서 골격계 및 흉부영상과 같은 고 공간분해능 임상영상을 얻기 위한 기초자료로 활용할 수 있다.

• 한국자원식물학회지
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• 제29권3호
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• pp.289-296
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• 2016

This study was executed to evaluate the immune activity, nitrite scavenging activity and ABTS radical scavenging activity against extracts of various concentration of ethanol solvent from Codonopsis lanceolata cultured at 6 local regions. The immune responses from both human T and B cell line was significantly enhanced in the cell growth compared to control while the cell growth was influenced at a certain period of culture. The results revealed that the cell growth of both human T and B cell was altered in a time dependent manner. The nitrite scavenging activity of ethanol extracts from various solvent concentration of C. lanceolata were affected by pH. At a pH of 1.2, the nitrite scavenging effect of all of the extracts tested observed higher than that of the other two pH ranges. There was no distinct detection of nitrite scavenging effects of the pH range 6.0. The ABTS radical scavenging activity was progressively increased in a dose-dependent manner, and the activity was the highest in 100% ethanol extract. The result from this investigation suggests that the extract of Codonopsis lanceolata could be an addition to basic medicine for immune modulation and natural food additives.

• 대한골관절종양학회지
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• 제3권2호
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• pp.69-79
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• 1997

Objective : The objective of this study was to compare expression of various proto-oncogenes and rates of apoptosis in osteosarcoma patients. Modulation of apoptosis may influence resistance to chemotherapy and therefore affect the outcome of cancer treatment. Osteosarcoma is one of the most fatal malignancies in young adolescents and investigation of the role of apoptotic cell death is warranted in relation to chemotherapy and tumor outcome. Design : The terminal deoxynucleotidyl transferase to exposed 3'-hydroxyl termini of DNA (TUNEL method) staining method has been applied for the in situ detection of DNA double strand breaks. Patients : Thirty-three osteosarcomas in various stages of differentiation from twenty-nine patients were investigated immunohistochemically for p53, Bcl-2 and TUNEL method for apoptosis. Results and conclusion; We have found that higher level of wild type p53 were correlated with enhanced expression of apoptosis. Increased apoptosis rates were found in cases of negative Bcl-2 expression. In the present study, we have concluded that a significant proportion of osteosarcoma, a tumor in which resistance to chemotherapy often occurs, express high levels of p53 and low levels of Bcl-2. Our data provide further evidence for cross-talk between Bcl-2 and p53 and suggests that these genes are important determinants of drug-induced apoptosis.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.465-468
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• 2008

본 연구는 영, 호남 지역의 레미콘 공장에서 현재 사용하고 있는 잔골재의 특성을 분석하고, 혼합사용 시 잔골재율 조정에 따른 콘크리트의 특성실험을 통하여 혼합사용 시 최적조건을 분석한 것으로 그 결과를 요약하면 다음과 같다. 영, 호남에서 사용하는 골재 종류별 물성은 조립률 및 0.08mm체 통과량을 제외하고 모두 KS F 2526 및 KS F 2527의 규정에 적합한 것으로 나타났는데, 각 공장에서는 단독사용이 부적합한 골재를 2종 이상 적절한 비율로 혼합사용하고, KS규정에 적합하게 품질을 관리하고 있었다. 영, 호남 각 공장에서 단위용적질량법에 의해 산출한 잔골재율로 조정한 콘크리트의 배합은 기존의 출하배합보다 높은 유동성을 보이는 것으로 나타났는데, 이는 잔골재율 약 2% 저감에 따라 잔골재의 표면적 감소 및 공극감소에 따른 시멘트 페이스트의 유동성에 미치는 잉여수량의 상대적 증가에 기인한 것으로 분석된다. 따라서 최적 잔골재율을 적용함으로써 단위수량 감소로 인한 경제적인 콘크리트 배합과 콘크리트의 내구성을 증진시키는 효과가 있을 것이라 사료된다.

• 한국식품영양과학회지
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• 제23권2호
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• pp.212-218
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• 1994

The effects of dietary buckwheat vegetables on the in vitro HMG-CoA reductase activity and lipids profiles of serum and liver in rats were evaluated . Buckwheat vegetables were cultivated in the range of 1-15cm of the plant height and dried. The content of rutin in the dried vegetables did not varied largely with the differences in the height of the vegetables. The water extract from buckwheat vegetables activited in vitro HMG-CoA reductase, activity up to 150% of control. Male rats were fed on purified diets. supplemented with0.12% cholesterol, containing dried buckwheat vegetables(BWV, 5% of diet) or rutin 90.25% of diet) . Dietary BWV and rutin, compared with basal diet tended to decrease the concentration of serum cholesterol. The ratio of HDL-cholesterol to total cholesterol were higher slightly in the rutin -fed group than in the other groups. The concentration of serum triacylglycerol was lower markedly in the rutin -fed groups than in the control groups, and BWV group being intermediate . The concentration of liver cholesterol was lower significantly in the BWV group than in the other groups. The results show an effective cholesterol-lowering action of buckwheat vegetables in serum and liver of rats fed on cholesterolemic diets. and that the hypocholesterolemic effects of buchwheat vegetables re not likely to be due to the modulation of the cholesterol synthesis rate.