• Title/Summary/Keyword: High-tech

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The Evaluation of Antithrombotic and Fibrinolytic Activities of Nattokinase from Bacillus subtilis Natto (Bacillus subtilis Natto가 생산하는 Nattokinase의 항혈전 및 피브린 용해능 효능평가)

  • Lee, Da-Lyung;Hong, Sung-Yu;Jang, Yang-Su;Jang, Hyung-Wook;Maeng, Chang-Jae;Yoo, Chul-Bae;Baek, Dae-Heoun
    • KSBB Journal
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    • v.27 no.6
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    • pp.375-380
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    • 2012
  • We previously reported that Ultra nattokinase$^{(R)}$ showed high fibrinolytic activity and revealed antithrombotic effect in rat blood plasma based on its ability to suppress collagen-induced platelet aggregation. This research was carried out to verify the clot lysing activity and blood flow enhancing effects of Ultra nattokinase$^{(R)}$ via monitoring and comparing the antithrombotic effects in rat artery between oral administration of Ultra nattokinase$^{(R)}$ and maltodextrin. SD rats were fed with 1.11 mg/kg of Ultra nattokinase$^{(R)}$ for 4 weeks. The effect on arterial thrombosis was then evaluated using an antithrombotic model after induction by $FeCl_3$. Detected fibrinolytic activity was proportional to the content of Ultra nattokinase$^{(R)}$ and statistical extents of the antithrombotic activity was enhanced strongly twice rather than control group. The PT and the aPTT, however, showed only a small difference between two groups. The results suggest that Ultra nattokinase$^{(R)}$ can effectively treat thromboembolism and enhance blood flow, and that Ultra nattokinase$^{(R)}$ can also prevent venous occlusion by aiding clot lysis.

Changes of physico-chemical properties in the reclaimed tidal land soils by precipitation (자연강우에 의한 간척지토양의 이화학적 특성변화)

  • Kim, Jae-Young;Son, Jae-Gwon;Koo, Ja-Woong;Choi, Jin-Kyu
    • Journal of Korean Society of Rural Planning
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    • v.8 no.1 s.15
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    • pp.3-14
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    • 2002
  • Changes of chemical properties by times of the reclaimed tidal land soils and soil surface water, underground infiltration water with precipitation-runoff on natural meteological condition in the unripened tidal reclaimed paddy fields were investigated. This study was carried out to use environment-friendly farm land in the reclaimed tidal lands. The soils used in this study were saline-alkaline soils with the high $Na^+$ and $Mg^{++}$ content. As the results of investigation outflow loading of nutriments through outflow water in the unripened tidal reclaimed paddy fields by precipitation during the survey period, nutriments equivalent to T-N $1{\sim}2\;kg\;10a^{ -1}$ and T-P $0.01{\sim}0.02\;kg\;10a^{-1}$ from in the unripened tidal lands were discharged. Besides, the results of comparison losses of cation through outflow water showed $Na^+>\;K^+>\;Mg^{++}\;>\;Ca^{++}$, and the highest appeared water discharge of $Na^+$. In case of saemangeum reclaimed tidal land soils water discharge of cations showed $Ca^{++}$ 1.3 kg $10a^{-1}$, $Mg^{++}$ 1.6 kg $10a^{-1}$, $Na^+$ 17.7 kg $10a^{-1}$, and $K^+$ 3.2 kg $10a^{-1}$ respectively. On the other hand, in case of koheung reclaimed tidal lands soils water discharge of cations showed $Ca^{++}$ 18.1 kg $10a^{-1}$, $Mg^{++}$ 31.2 kg $10a^{-1}$, $Na^+$ 320.8 kg $10a^{-1}$ and $K^+$ 51.2 kg $10a^{-1}$ respectively.

A Study on the Passive House Technology Application of University Dormitory through The House at Cornell Tech (코넬 공과대학 기숙사 사례를 통한 대학 기숙사의 패시브 하우스 기술 적용에 관한 연구)

  • Kim, Hong-Min;Oh, Hyoung-Seok;Ryu, Soo-Hoon
    • Journal of the Korean Institute of Educational Facilities
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    • v.25 no.4
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    • pp.11-18
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    • 2018
  • Global warming is happening now and inevitable. Everyone knows that immediate action should be taken to slow it down, but uncertain about the effective solution. Despite global efforts to reduce greenhouse gas emissions, sea levels are rising gradually. In 2013, Cornell University announced the Climate Action Plan(CAP) to make the campus greener, to reduce waste, and to ensure efficient use of resources. In particular, they set a goal of reducing energy use by 2050 and making carbon emissions to zero. Accordingly, the purpose of this study is to analyse the case of the master plan of Cornell Tech campus and its major buildings. Mainly, The House, faculty and student housing of Cornell Tech and the world tallest certified passive house, will be the main precedent that shows the architectural planning of passive house. Passive house technology, which was thought to be possible only in single-family houses, can be applied to high-rise buildings. If any passive house technology of The House project is actively introduced into the dormitory projects of domestic universities that are about to be built or renovated, it will be a good opportunity for the university to take the lead in preparing for global warming.

Molecular Characterization of the Levansucrase Gene from Pseudomonas aurantiaca S-4380 and Its Expression in Escherichia coli

  • Jang, Eun-Kyung;Jang, Ki-Hyo;Isaac Koh;Kim, In-Hwan;Kim, Seung-Hwan;Kang, Soon-Ah;Kim, Chul-Ho;Ha, Sang-Do;Rhee, Sang-Ki
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.603-609
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    • 2002
  • DFA IV is di-D-fructose-2,6':6,2'-dianhydride, consisting of two fructose residues. It can be enzymatically synthesized from levan by levan fructotransferase, and can be used for mineral absorption. Understanding of the structure and composition of levan is important to obtain high-level production of DFA IV. A bacterial strain, Pseudomonas aurantiaca 5-4380, was identified to produce low-branched levan, and the levansucrase gene (lsch) from this bacterium was found to be composed of 1,275 Up coding for a protein of 424 amino acids, with an estimated molecular weight of 47 kDa. The bacterial levansucrase gene was expressed in Escherichia coli DH5${\alpha}$ by its own promoter and lac promoter. The recombinant levansucrase was produced in soluble form with 170U of levansucrase activity from 1-ml E. coii culture broth. The expressed enzyme from the clone showed similar biochemical properties, such as size of active levansucrase, degree of branching, and optimum temperature, with P.aurantiaca 5-4380 levansucrase.

Evaluation of Nattokinase for Antithrombotic Effect and Pharmacological Efficacy by a Biological Test and Clinical Trial (동물 및 인체시험을 통한 Nattokinase의 항응고 작용 및 섬유소 용해능 평가)

  • Kim, Jae-Bum;Yoo, Chul-Bae;Shin, Hyun-Man;Jung, Joon-Ki;Jang, Hyung-Wook
    • KSBB Journal
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    • v.26 no.5
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    • pp.393-399
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    • 2011
  • Bacillus subtilis natto producing high level of a fibrinolytic enzyme was selected and Ultra Nattokinase$^{(R)}$ was manufactured by fermentation and purification. It was performed the evaluation of the antithrombotic effect of Ultra Nattokinase$^{(R)}$ (20,000 FU/g) with rat blood plasma. The maximum aggregation (inhibition ratio) was 71% (0%), 69% (2.8%), 62% (12.7%), 16% (77.5%) and 9% (87.3%), respectively, in the order of 0, 5, 10, 50 and 100 mg/mL of Ultra Nattokinase$^{(R)}$ solutions. Ultra Nattokinase$^{(R)}$ had antithrombotic effect, which was associated with the suppression of collagen-induced platelet aggregation. Ultra Nattokinase$^{(R)}$ in the topic of the FDP (fibrinogen degradation products) in blood coagulation tests showed a significant increasing trend. And based on the daily record of meal 39 people of ITT (what ?) group consisted with 19 people of NP (what ?) group and 20 people of PN (what ?) group except four people, two people who took vitamin K affecting the experiment and two people who took alcohol, finding to be taken Ultra Nattokinase$^{(R)}$ showed an increase in the FDP value after four weeks. In addition, FDP value of 41 people of ITT group except two people having metabolic syndrome was increased by Ultra Nattokinase$^{(R)}$.

The Magnetic Properties of Co-Ni-Fe-N Soft Magnetic Thin Films

  • Kim, Y. M.;Park, D.;Kim, K. H.;Kim, J.;S. H. Han;Kim, H. J.
    • Journal of Magnetics
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    • v.5 no.4
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    • pp.120-123
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    • 2000
  • Co-Ni-Fe-N thin films were fabricated by a $N_2$ reactive rf magnetron sputtering method. The nitrogen partial pressure ($P_{N2}$) was varied in the range 0~10% . As$P_{N2}$ increases in this range, the saturation magnetization $B_s$ linearly decreases from 19.8 kG to 14 kG and the electrical resistivity ($\rho$) increases from 27 to 155 $\mu\Omegacm$. The coercivity $H_c$ exhibits the minimum value at 4% $P_{N2}$. The magnetic anisotropy fields ($H_k$) are in the range of 20$\sim$50 Oe. High frequency characteristics of $(Co_{22.2}Ni_{27.6}Fe_{50.2})_{100-x}N_x$ films are excellent in the range of 3$\sim$5% of $P_{N2}$. In particular, the effective permeability of the film fabricated at 4% $P_{N2}$ is 800, which is maintained up to 600 MHz. This film also shows Bs of 17.5 kG, $H_c$/ of 1.4 Oe, resistivity of 98$\mu\Omegacm$ and $H_k$ of about 25 Oe. Also, the corrosion resistance of $(Co_{22.2}Ni_{27.6}Fe_{50.2})_{100-x}N_x$ films was imp roved with increasing N concentration.

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Millennial parents' perception of babywearing products: A text analysis approach (밀레니얼 세대의 Babywearing 제품에 대한 인식: 텍스트 분석 접근)

  • Lee, Wan-Gee;Park, Myung-Ja;Lee, Kyu-Hye
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.2
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    • pp.17-28
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    • 2021
  • The baby-tech industry, which combines IT with existing parenting product, is attracting increasing amounts of attention. Consequently various types of baby products incorporating functionality and design are being launched. In recent years, particularly as the market segments increases for babywearing products, parenting products that account for the child's comfort and parents' convenience are required. Therefore, this study examines the characteristics and consumer perception of babywear products, which are important for the emotional stability, development, and rearing of children. The study utilizes text mining and a network analysis by collecting unstructured text data. An examination of the network, based on the frequency of keywords for each babywear product and the degree of the connection to the centering index, revealed that consumers value convenience and price when purchasing products. The consumer perception and consideration factors that appear individually according to the product were also identified. In addition, studying body parts with high TF-IDF values revealed a difference in the body parts considered by consumers for each product. Lastly, through the visualization data based on the keywords that appeared in public, commonly appearing keywords, and those that appeared individually were examined. Through SNS, product characteristics as well as a new parenting culture that shared child-rearing routines were confirmed. This study suggests planning and marketing directions for the development of babywear products that meet consumer needs.

A Study on Non-contact Penetration and Rebound Measurement Device for Quality Control in Driven Piles (말뚝 시공관리를 위한 비접촉식 관입량 측정장치 활용에 관한 연구)

  • Seo, Seunghwan;Kim, Juhyong;Choi, Changho;Chung, Moonkyung
    • Journal of the Korean Geotechnical Society
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    • v.38 no.11
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    • pp.97-106
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    • 2022
  • The domestic auger-drilled pile method generally manages the driving penetration (set) value with the final stage of construction. The penetration value has been estimated by manual measurement for a long time. The automation technology is yet to be applied due to workability and high-cost limitations, despite safety issues and lack of reliability in measured results. In this study, a non-contact pile penetration measurement device was developed. Further, the field performance was verified by comparing the measurements with a conventional automation device. In addition, the on-site field quality control method was analyzed using the penetration measuring device. The field experiments confirmed that more reliable bearing capacity estimation could calculate the dynamic damping coefficient and the modified Hiley formula with the developed device. Furthermore, it can be used for pile construction management from the bearing capacity viewpoint, even for piles not subjected to dynamic load tests. 

Feasibility Analysis of Exploring Underground Utilities Using Muon (뮤온 입자를 활용한 지하매설물 탐사 가능성 분석)

  • Seo, Seunghwan;Chung, Moonkyung;Kwak, Kiseok;Kang, Jae Mo
    • Journal of the Korean Geotechnical Society
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    • v.38 no.11
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    • pp.137-147
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    • 2022
  • Various geophysical exploration methods are used to determine the exact location of underground utilities, and many studies have been performed to improve the accuracy. This study analyzed the feasibility of exploring underground utilities through a new exploration method using cosmic ray muon. A prototype of a portable muon detector was manufactured by combining a scintillator and a silicon photomultiplier. Further, a calibration operation was performed on the muon count rate. The ground thickness of the ground model was measured using the muon detector prototype, where the value could be estimated with an error of about 3%, close to the actual. In addition, the theoretical basis for tomography analysis technology was analyzed to utilize the muon detector for exploring underground utilities, and a zenith angle correction method was presented. This study revealed that the technology of exploration using muon can analyze density with high resolution and will be used for exploring underground utilities.

THE MAGNETIC PROPERTIES OF Co-Ni-Fe-N SOFT MAGNETIC THIN FILMS

  • Kim, Y. M.;Park, D.;Kim, K. H.;Kim, J.;S. H. Han;Kim, H. J.
    • Proceedings of the Korean Magnestics Society Conference
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    • 2000.09a
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    • pp.492-499
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    • 2000
  • Co-Ni-Fe-N thin films were fabricated by a N$\sub$2/ reactive rf magnetron sputtering method. The nitrogen partial pressure (P$\sub$N2/) was varied in the range of 0∼10%. As P$\sub$N2/ increases in this range, the saturation magnetization (B$\sub$s/) linearly decreases from 19.8 kG to 14 kG and the electrical resistivity ($\rho$) increased from 27 to 155 ${\mu}$$\Omega$cm. The coercivity (H$\sub$c/) exhibits the minimum value at 4% of P$\sub$N2/. The magnetic anisotropy (H$\sub$k/) are in the range of 20∼50 Oe. High frequency characteristics of (Co$\sub$22.2/Ni$\sub$27.6/Fe$\sub$50.2/)$\sub$100-x/N$\sub$x/ films are excellent in the range of 3∼5% of P$\sub$N2/. Especially the effective permeability of the film fabricated at 4% of P$\sub$N2/ is 800, which is maintained up to 600 MHz. This film also shows Bs of 17.5 kG, H$\sub$c/ of 1.4 Oe, resistivity of 98 $\Omega$cm and H$\sub$k/ of about 25 Oe. Also, the corrosion resistance of (Co$\sub$22.2/Ni$\sub$27.6/Fe$\sub$50.2/)$\sub$100-x/N$\sub$x/ were improved with the increase in N concentration.

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