• The Korean Journal of Pesticide Science
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• v.5 no.3
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• pp.41-46
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• 2001

This study was conducted to develop a high throughput system for screening acetolactate synthase(ALS) inhibitors, and to detect basic mother molecules for developing new novel herbicide candidates. The high throughput screening (HTS) method using 96-well plate and microplate reader was developed. This method is 8 times more effective than basic technique in one cycle per person. Futhermore, considering for less than 1/10 volume of materials required for ALS test and enzyme kinetics with 16 times faster speed compared to those of former procedure, this HTS method has more than 100 times higher efficacy than basic system in a consecutive procedure. We discovered 11 new ALS inhibitors such as 2-oxoglutaric acid, aminooxyacetic acid, azelaic acid, citric acid, cyanuric fluoride, itaconic acid, malonic acid, niclosamide, oxalic acid, glyoxylic acid, and suramin from 107 commercial plant-specific inhibitors using this technique. We hope these results might be useful to discover lead compounds for developing new novel herbicide candidate.

• Journal of the Korea Safety Management & Science
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• v.15 no.1
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• pp.293-301
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• 2013

The purpose of this study is to introduce the limit of previously used six sigma quality process evaluation metrics, $Z_{st}$ and $P_{pk}$, and a solution to overcome this drawback by using a metric based on performance evaluation of Z-factor quality innovation. Case analysis on projects from national six sigma contest from 2011 to 2012 is performed and literature review on new drug development HTS (High Throughput Screening) is used to propose innovative performance evaluation metrics. This research shows that experimental study on six sigma evaluation metric, $Z_{st}$ and $P_{pk}$, have no significance difference between industrial type (Manufacturing, Semi-Public Institute, Public Institute) and CTQ type (Product Technology Type CTQ, Process Technology Type CTQ). Following discovery characterize this quality improvement as fixed target type project. As newly developed moving target type of quality innovation performance metric Z-Factor is used for evaluating experimental study, hypothetical analysis suggests that $Z_{st}$ and $P_{pk}$ share different relationship or even show reciprocal relationship. Constraints of the study are relatively small sample size of only 37 projects from past 2 years and conflict on having interview and communication with six sigma quality practitioner for qualitative experimental study. Both moving target type six sigma innovation project and fixed target type improvement project or quality circle enables efficient ways for a better understanding and quality practitioner use by applying quality innovation performance metric. Downside of fixed target type quality performance evaluation metric, $Z_{st}$ and $P_{pk}$, is presented through experimental study. In contrast, advantage of this study is that high throughput requiring product technology, process technology and quantum leap typed innovation effect is evaluated based on precision and accuracy and Z-Factor that enables relative comparison between enterprises is proposed and implemented.

• The KIPS Transactions:PartA
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• v.8A no.2
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• pp.157-166
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• 2001

A simulation package has been developed as an event-driven system that can handle the hardware configuration of STC104 and algorithm proposed in the sister paper of ‘Study on High Speed Routers(II).’After various STC104 topology of meshes, torus, and hypercubes are constructed using up to 512 switches, the performance of each topology has been analyzed under different message generation rate in terms of throughputs, latency, and packet blocking time. Modified multicast algorithms for STC104 have been proposed for STC104 after U-mesh and U-torus in order to overcome the multicasting difficulty because of the point-to-point communication method found in STC104. The performance of the multicast algorithms have been analyzed over meshes and torus configuration. Throughput gets higher in the order of mesh, torus, and hypercube. Throughput difference among topology were distinctive in the zone of high message generation rate. Latency and blocking time increased in the order of hypercube, torus, and mesh. U-mesh and U-torus of software multicast showed similar throughput, however, U-mesh peformed slightly better result. These algorithms showed eight to ten times better results compared to individual message pass for 90 destination nodes. Multi-link environment also showed better performance than single-link environment because multi-link network used the extra links for communication.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.3C
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• pp.287-294
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• 2010

MB-OFDM UWB system will adopt LDPC codes to enhance the decoding performance with higher data rates. In this paper, we will consider algorithm and architecture of the LDPC codes in MB-OFDM UWB system. To suggest the hardware efficient LDPC decoder architecture, LLR(log-likelihood-ration) calculation algorithms and check node update algorithms are analyzed. And we proposed the architecture of LDPC decoder for the high throughput application of Wimedia UWB. We estimated the feasibility of the proposed architecture by implementation in a FPGA. The implementation results show our architecture attains higher throughput than other result of QC-LDPC case. Using this architecture, we can implement LDPC decoder for high throughput transmission, but it is 0.2dB inferior to the BP algorithm.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.12
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• pp.4648-4663
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• 2020

This paper proposes the Lempel-Ziv 4(LZ4) compression accelerator optimized for scale-out servers in data centers. In order to reduce CPU loads caused by compression, we propose an accelerator solution and implement the accelerator on an Field Programmable Gate Array(FPGA) as heterogeneous computing. The LZ4 compression hardware accelerator is a fully pipelined architecture and applies 16 dictionaries to enhance the parallelism for high throughput compressor. Our hardware accelerator is based on the 20-stage pipeline and dictionary architecture, highly customized to LZ4 compression algorithm and parallel hardware implementation. Proposing dictionary architecture allows achieving high throughput by comparing input sequences in multiple dictionaries simultaneously compared to a single dictionary. The experimental results provide the high throughput with intensively optimized in the FPGA. Additionally, we compare our implementation to CPU implementation results of LZ4 to provide insights on FPGA-based data centers. The proposed accelerator achieves the compression throughput of 639MB/s with fine parallelism to be deployed into scale-out servers. This approach enables the low power Intel Atom processor to realize the Hadoop storage along with the compression accelerator.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.2
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• pp.69-75
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• 2004

In recent years, the importance of proteomic works, such as protein expression, detection and identification, has grown in the fields of proteomic and diagnostic research. This is because complete genome sequences of humans, and other organisms, progress as cellular processing and controlling are performed by proteins as well as DNA or RNA. However, conventional I protein analyses are time-consuming; therefore, high throughput protein analysis methods, which allow fast, direct and quantitative detection, are needed. These are so-called protein microarrays or protein chips, which have been developed to fulfill the need for high-throughput protein analyses. Although protein arrays are still in their infancy, technical development in immobilizing proteins in their native conformation on arrays, and the development of more sensitive detection methods, will facilitate the rapid deployment of protein arrays as high-throughput protein assay tools in proteomics and diagnostics. This review summarizes the basic technologies that are needed in the fabrication of protein arrays and their recent applications.

• ETRI Journal
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• v.30 no.5
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• pp.707-717
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• 2008

This paper presents two types of high-speed hardware architectures for the block cipher ARIA. First, the loop architectures for feedback modes are presented. Area-throughput trade-offs are evaluated depending on the S-box implementation by using look-up tables or combinational logic which involves composite field arithmetic. The sub-pipelined architectures for non-feedback modes are also described. With loop unrolling, inner and outer round pipelining techniques, and S-box implementation using composite field arithmetic over $GF(2^4)^2$, throughputs of 16 Gbps to 43 Gbps are achievable in a 0.25 ${\mu}m$ CMOS technology. This is the first sub-pipelined architecture of ARIA for high throughput to date.

• BMB Reports
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• v.50 no.4
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• pp.201-207
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• 2017

Alternations in usage of polyadenylation sites during transcription termination yield transcript isoforms from a gene. Recent findings of transcriptome-wide alternative polyadenylation (APA) as a molecular response to changes in biology position APA not only as a molecular event of early transcriptional termination but also as a cellular regulatory step affecting various biological pathways. With the development of high-throughput profiling technologies at a single nucleotide level and their applications targeted to the 3'-end of mRNAs, dynamics in the landscape of mRNA 3'-end is measureable at a global scale. In this review, methods and technologies that have been adopted to study APA events are discussed. In addition, various bioinformatics algorithms for APA isoform analysis using publicly available RNA-seq datasets are introduced.

• KSBB Journal
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• v.28 no.3
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• pp.213-215
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• 2013

A high-throughput screening strategy was developed to simplify the selection process for improved mannitol producing strain after chemical mutagenesis. Ethylmethyl sulfonate (EMS) was used as a chemical mutagen to alter the fructokinase-I gene which is an essential enzyme to metabolize fructose for growth. Leuconostoc mesenteroides treated with EMS were plated on the modified MRS solid medium containing fructose as a sole carbon source. Strains showing inhibited growth were primarily selected to evaluate the mannitol producing ability. By applying this strategy, L. mesenteroides ATCC 8293 M1, L. mesenteroides ATCC 9135 M3 and L. mesenteroides D1 M3 showed improvement in mannitol production.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2007.04a
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• pp.719-724
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• 2007

Many engineering problems often require the large amount of computing resources for iterative simulations of problems treating many parameters and input files. In order to overcome the situation, this paper proposes an e-Science based computational system. The system exploits the Grid computing technology to establish an integrated web service environment which supports distributed high throughput computational simulations and remote executions. The proposed system provides an easy-to-use parametric study service where a computational service includes real time monitoring. To verify usability of the proposed system, two kinds of applications were introduced. The first application is an Aerospace Integrated Research System (e-AIRS). The e-AIRS adapts the proposed computational system to solve CFD problems. The second one is design and optimization of protein 3-dimensional structures.