• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2002.10b
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• pp.111-112
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• 2002

The influence of aerated oil on a high-speed proceeding bearing is examined by using the classical thermohydrodynamic lubrication theory coupled with analytical models for viscosity and density of air-oil mixture in fluid-film bearing including the live surface tension of aerated oil. Convection to the walls and mixing with supply oil and re-circulating oil are considered. The considered parameters for the study of bubbly lubrication are oil aeration level, air bubble size and shaft speed. The results show that, if the live surface tension is considered, the effect of air bubbles on the bearing load capacity is reduced due to temperature engagement comparing with that under the condition of a constant surface tension.

• Environmental Engineering Research
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• v.21 no.3
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• pp.233-240
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• 2016

The research aims to study the different flexible rubber tube diffusers used in urban wastewater treatment processes and aquaculture systems. The experiment was conducted in small-scale aeration tank with different physical properties of the tubes that were used as aerators. The volumetric mass transfer coefficient ($k_La$), oxygen transfer efficiency (OTE) and aeration efficiency (AE) were measured and determined to compare the diffusers. Moreover, the bubble hydrodynamic parameters were analyzed in terms of bubble diameter ($d_B$) and rising velocity ($U_B$) by a high speed camera (2,000 frames/s). Then the interfacial area (a) and liquid-side mass transfer coefficient ($k_L$) can be calculated. The physical properties (tube wall thickness, tensile strength, orifice size, hardness and elongation) have been proven to be the key factor that controls the performance (kLa and OTE). The effects of hardness and elongation on bubble formation, orifice size and a-area were clearly proved. It is not necessary to generate too much fine bubbles to increase the a-area: this relates to high power consumption and the decrease of the kL. Finally, the wall thickness, elongation and hardness associated of the flexible tube diffuser (tube No. 12) were concluded, to be the suitable properties for practically producing, in this research.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.548-553
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• 1999

A production of $\beta-Carotene$was attempted in a fed-batch culture of Blakeslea trispora by controlling both foam and dissolved oxygen in the presence of surfactant, Span 20. Results obtained from the shake flask cultures indicated that a high concentration of dissolved oxygen was needed for both cell growth and $\beta-Carotene$ synthesis, and the optimal concentration of glucose was found to be in the range of 50-100 g/l. In order to maintain the dissolved oxygen concentration level at higher than 50% of air saturation, pure oxygen was automatically sparged into the medium with air. Foam was controlled by bypassing air from the submerged aeration to the headspace in response to the foam that was caused by Span 20. High agitation speed was found to be detrimental to the cell growth due to shear damage, even though it provided sufficient dissolved oxygen. On the other hand, a low aeration speed caused stagnant regions in the fermentor because of improper mixing. Thus, for the fed-batch operation, agitation speed was increased gradually from 300 to 700 rpm to prevent cell damage at the initial stage of fermentation and to give efficient mixing for a viscous culture broth as the culture proceeded. By controlling dissolved oxygen and foam, a high concentration of $\beta-Carotene$otene (1,190 mg/l) was obtained in 6 days of the fed-batch culture of B. trispora with 2.5% of the dry cell weight, which was approximately 5 times higher than that of the batch cultures.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.3
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• pp.205-212
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• 2000

Experimental investigations on the removal of protein, total suspended solids and turbidity from aquacultural water were carried out by using three types of foam separator: counter current air driven type foam separator (CCADFS), high speed aeration type foam separator (HSAFS) and venturi type foam separator (VFS). The decrease of flow rate by CCADFS, HSAFS and VFS were $0.4,\;66.1,\;77.2 {\%}$ respectively. Protein removal rates by three types foam separator were decreased with the increased hydraulic residence time (HRT). Bellw 0.32 minute and 0.21 minute of hydraulic residence times, protein removal rate of HSAFS and YES was higher than that of CCADFS, respectively. Protein removal rate of VFS was lower than that of HSAFS at any HRT. As increasing the HRT, protein removal efficiency of CCADFS was increased, but that of HSAFS and VES were decreased. The changes of removal rates and efficiencies of total suspended solid and turbidity were similar to proteins.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.389-394
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• 1994

8 X 10$^{6}$(viable cells/ml) of maximum cell density and 9000(IU/ml) of $\gamma$-IFN production were obtained at 55(ml/hr) of a perfusion rate by cultivating HSF cells using a moving membrane aeration bioreactor. This system proves to be an efficient culture process by maintaning 90% of viable cells during the whole cultivation periods. The metabolic molar quotient of glucose to lactate was 0.81 for overall ranges of glucose consumed while the evolution of ammonia was not linearly related to the consumption of glutamine. Low molar conversion ratio was observed in low consumptions of glutamine and high molar conversion ratio in high comsumptions. It also shows that the glutamolysis plays important role in the steady state conditions by evolving larger quantities of ammonia than lactate. At the above of 50 rpm, which is the optimum agitation speed for this bioreactor, the cell growth was severely affected while the IFN production was less decrea- sed, maintaing 1.5 X 10$^{-3}$(IU/cell/day) specific IFN production rate. The cumulatvie $\gamma$-IFN production was 7.2 X 10$^{8}$(IU) for 70 days of the cultivation, which yields 1 X 10$^{7}$ (IU/day) of IFN production rate. Therefore, a commercial production of $\gamma$-IFN by this culture process can be achievable by maintaining the above IFN productivity in a scaled-up culture system.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1690-1698
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• 2006

In order to investigate the effect of dissolved oxygen (DO) level on AVM $B_{1a}$ production by a high yielding mutant of Streptomyces avermitilis, five sets of bioreactor cultures were performed under variously controlled DO levels. Using an online computer control system, the agitation speed and aeration rate were automatically controlled in an adaptive manner, responding timely to the oxygen requirement of the producer microorganism. In the two cultures of DO limitation, the onset of AVM $B_{1a}$ biosynthesis was observed to casually coincide with the fermentation time when oxygen-limited conditions were overcome by the producing microorganism. In contrast, this phenomenon did not occur in the parallel fermentations with DO levels controlled at around 30% and 40% throughout the entire fermentation period, showing an almost growth-associated mode of AVM $B_{1a}$ production: AVM $B_{1a}$ biosynthesis under the environments of high DO levels started much earlier than the corresponding oxygen-limited cultures, leading to a significant enhancement of AVM $B_{1a}$ production during the exponential stage. Consequently, approximately 6-fold and 9-fold increases in the final AVM $B_{1a}$ production were obtained in 30% and 40% DO-controlled fermentations, respectively, especially when compared with the culture of severe DO limitation (the culture with 0% DO level during the exponential phase). The production yield ($Y_{p/x}$), volumetric production rate (Qp), and specific production rate (${\bar{q}}_p$) of the 40% DO-controlled culture were observed to be 14%, 15%, and 15% higher, respectively, than those of the parallel cultures that were performed under an excessive agitation speed (350 rpm) and aeration rate (1 vvm) to maintain sufficiently high DO levels throughout the entire fermentation period. These results suggest that high shear damage of the high-yielding strain due to an excessive agitation speed is the primary reason for the reduction of the AVM $B_{1a}$ biosynthetic capability of the producer. As for the cell growth, exponential growth patterns during the initial 3 days were observed in the fermentations of sufficient DO levels, whereas almost linear patterns of cell growth were observed in the other two cultures of DO limitation during the identical period, resulting in apparently lower amounts of DCW. These results led us to conclude that maintenance of optimum DO levels, but not too high to cause potential shear damage on the producer, was crucial not only for the cell growth, but also for the enhanced production of AVM $B_{1a}$ by the filamentous mycelial cells of Streptomyces avermitilis.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1603-1611
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• 2009

High-cell-density cultivation of yeast was investigated using the agricultural waste products corn steep liquor (CSL) and molasses. The Saccharomyces cerevisiae KV-25 cell mass was significantly dependent on the ratio between C and N sources. The concentrations of molasses and CSL in the culture medium were statistically optimized at 10.25% (v/v) and 16.87% (v/v), respectively, by response surface methodology (RSM). Batch culture in a 5-l stirred tank reactor using the optimized medium resulted in a cell mass production of 36.5 g/l. In the fed-batch culture, the feed phase was preceded by a batch phase using the optimized medium, and a very high dried-cell-mass yield of 187.63 g/l was successfully attained by feeding a mixture of 20% (v/v) molasses and 80% (v/v) CSL at a rate of 22 ml/h. In this system, the production of cell mass depended mainly on the agitation speed, the composition of the feed medium, and the glucose level in the medium, but only slightly on the aeration rate.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.284-285
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• 2000

분산된 공기 기포에 의해 고형물을 제거하는 포말분리법은 30 $\mu\textrm{m}$이하의 미세 입자와 용존 고형물을 매우 효율적이면서도 경제적으로 운전을 할 수 있고 연속 운전이 가능하며 역세척이 필요 없다는 장점을 지니고 있다((Clarke and Wilson. 1983). 현재 사용되고 있는 포말분리장치는 공기의 공급방식과 유체과 공기의 접촉방향에 따라 항류 공기구동식(counter current air driven type, CCAD), 고속 폭기식(high speed aeration type, HSA), 벤튜리식(venturi type) 등이 있으나 현재까지 우리나라에서 연구되어 오고있는 포말분리 장치는 공기구동 방식이 전부이다. (중략)

• Journal of Mushroom
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• v.6 no.1
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• pp.27-31
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• 2008

The optimization of submerged culture conditions for mycelial growth and exopolysaccharide (EPS) production in an edible mushroom Tremella fuciformis were studied in shake flasks and bioreactors. The temperature of $28^{\circ}C$ and pH 8 in the beginning of fermentation in agitated flasks was the most efficient condition to obtain maximum mycelial biomass and EPS. The optimal medium constituents were as follows (g l-1): glucose 20, tryptone 2, $KH_2PO_4$ 0.46, $K_2HPO_4$ 1 and $MgSO_4H_2O$ 0.5. The fungus was cultivated under various agitation and aeration conditions in a 5L stirred-tank bioreactor. The maximum cell mass and EPS production were obtained at a relatively high agitation speed of 200 rpm and at an aeration rate of 2 vvm. The flow behavior of the fermentation broth was Newtonian and the maximum apparent viscosity (35 cP) was observed at a highly aerated condition (2 vvm). The EPS productivity in an airlift reactor was higher than that in the stirred-tank reactor. The EPS was protein-bound polysaccharides consisted of mainly mannose, xylose, and fructose. The molecular weights of EPS were determined to be $1.3{\sim}1.5{\times}10^6$.

• Journal of Microbiology and Biotechnology
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• v.4 no.3
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• pp.222-229
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• 1994

In order to develop a method of economical production and to reduce energy-consumption in fuel alcohol production, we investigated the fermentation characters of two newly selected thermotolerant yeasts. The RA-74-2 showed stable and superior fermentability between 30 and $40^{\circ}C$ in 20% glucose media in comparison to the industrial strains. The optimum concentration of glucose for economical fermentation at $40^{\circ}C$ was 15-18%, and organic nitrogen was necessary for a satisfactory fermentation. The optimum pH was 4.0 and aeration was adversed for high temperature fermentation. Agitation was an important factor at $40^{\circ}C$ and the addition of magnesium ion 0.2% was required in this experiment. When the inoculum was increased, ethanol productivity as well as the speed of fermentation increased. On the other hand RA-912, which can grow at $48^{\circ}C$, showed similar fermentability between 30-$45^{\circ}C$ in 20% glucose media As the concentration of substrate decreased, fermentation ratio increased at $45^{\circ}C$ (45%, 65%, 95% fermentation ratio in 20%, 15%, 10% glucose media, respectively). Also, requirement of organic nitrogen and magnesium ion in RA-912 was similar in RA-74-2. The optimum pH for fermentation was 5.0, and the effects of agitation were enhanced at $37^{\circ}C$ than at $45^{\circ}C$. As the inoculum was increased, fermentation speed became more enhanced but the ethanol productivity was less affected. RA-912 showed fermentability with various substrates. Among the substrates used, inulin was the most promising substrate for the high-temperature fermentation. When 14.5% inulin was used as the substrate, 93% and 55% fermentation ratios were shown at $37^{\circ}C$ and $45^{\circ}C$, respectively.