• Title/Summary/Keyword: High level laser

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Utilization of functionalized magnetic nanoparticles for high throughput DNA separation

  • Jang, Jeong-Ho
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.11a
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    • pp.12.2-12.2
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    • 2009
  • The work describes anoptimized process to highly efficient and convenient preparation in highthroughput magnetic human DNA separation with chemically functionalizedsilica-coated magnetic nanoparticles. The effect of nanoparticle's size and the surface's hydrophilicity change were studied for magnetic DNA separation process, inwhich the optimum efficiency was explored via the function of the amino-groupnumbers, particle size, the amount of the nanoparticles used, and theconcentration of NaCl salt. The DNA adsorption yields were high in terms of theamount of triamino-functionalized nanoparticles used, and the average particlesize was 25 nm. The adsorption efficiency of aminofunctionalized nanoparticleswas the 4-5 times (80-100%) higher compared to silica-coated nanoparticles only(10-20%). DNA desorption efficiency showed an optimum level of over 0.7 M ofthe NaCl concentration. To elucidate the agglomeration of nanoparticles afterelectrostatic interaction, the Guinier plots were calculated from small angleX-ray diffractions in a comparison of the results of electron diffraction TEM,and confocal laser scanning microscopy. Additionally, the direct separation ofhuman genomic DNA was achieved from human saliva and whole blood with highefficiency.

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Measurement of Birefringence Distribution in Optical Disk Substrates Fabricated by Injection-Compression Molding (사출압축성형을 통한 광디스크 기판 성형 및 복굴절의 측정)

  • 김종성
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1999.03b
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    • pp.218-224
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    • 1999
  • It is necessary to improve mechanical and optical properties in the optical disk substrates as the information storage devices with high storage density using short wavelength laser are being developed. Injection compression molding is regarded as the most suitable process to manufacture optical disk substrates with high is regarded as the most suitable process to manufacture optical disk substrates with high dimensional accuracy low residual stresses and superb optical properties In the present study polycarbonate optical disk substrates were fabricated by injection compression molding and the birefringence regarded as one of the most important optical properties for optical disk is measured. The effects of various processing conditions upon the development of birefringence distribution were examined experimentally. It was found that the value of the birefringence distribution were very sensitive to the mold wall temperature history and the variance of the birefringence distribution in the radial direction was affected by the level of the packing and the compression pressure.

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Spray Characteristics of the Pressure Swirl Injector at Airplane Operating Conditions (항공기 작동조건에 따른 압력식 스월 인젝터의 분무특성 연구)

  • Choi, Chea-Hong;Choi, Seong-Man;Rhee, Dong-Ho
    • 한국전산유체공학회:학술대회논문집
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    • 2008.03b
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    • pp.691-694
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    • 2008
  • Spray characteristics of the APU simplex fuel nozzle are investigated. Four flight conditions such as sea level idle, sea level max power, 20,000 feet idle, 20,000 feet max power are used as spray experimental conditions. Spray visualization was performed by using ND-YAG laser. Droplet size and velocity were measured by using PDPA(Phase Doppler Particle Analyzer) system. From the test result, SMD is 100 ${\mu}$m�� and velocity is 10 m/s at 20,000 ft idle condition. In this condition, flame unstability could be occurred due to the higher drop diameter. Therefore it is necessary to decrease the droplet diameter in the high altitude condition.

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Analysis of Technical Requirement for Implementation of Multi-trade Prefabrication (Multi-trade Prefabrication 기법 적용을 위한 기술적 요구사항 분석)

  • Jang, Se-Jun;Lee, Ghang
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2016.05a
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    • pp.113-114
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    • 2016
  • This paper proposes a technical requirements analysis of implementation of multi-trade prefabrication. Recently, there has been a rise in the use of prefabrication to minimize on-site work for time reduction to increase productivity. Prefabrication technique is evolved into multi-trade prefabrication combining other trades from single-trade prefabrication. For implementation of new technique, not only itself but complementary techniques have to be prepared. In this paper, MEP corridor rack, a major item of multi-trade prefabrication, was implemented in the test bed and its process was analyzed to find out technical requirements. As a result, comparatively high level of IT technique was required for efficient use of multi-trade prefabrication in design, lifting and construction phase. In design phase, component level of BIM library was needed for manufacturing; and in lifting phase, BIM-based site logistics process was required. Also in construction phase, laser scanning was implemented for gathering shape and geometry of the wall and slab that were attached to multi-trade prefabrication module.

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Study on visualization of vortex flow on hydrofoils (수중익에서 발생하는 보텍스 유동 가시화 연구)

  • Hong, Ji-Woo;Ahn, Byoung-Kwon
    • Journal of the Korean Society of Visualization
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    • v.19 no.2
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    • pp.48-55
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    • 2021
  • In order to design a propeller with high efficiency and excellent cavitation performance, theoretical and experimental studies on the cavitation and noise characteristics according to the blade section shape are essential. In general, sheet cavitation, bubble cavitation, and cloud cavitation are the main causes of hull vibration and propeller surface erosion. However vortex cavitation, which has the greatest influence on the noise level because the fastest CIS in ship propeller, has been researched for a long time and studies have been conducted recently to control it. In this experiment, the development process of cavitation was measured by using three dimensional wings with two different wing section and wing tip shapes, and the noise level at that time was evaluated. In addition, we evaluated the relationship between cavitation inception and hydrodynamic force using three component load cell and we measured the velocity field of wing wake using LDV.

Fabrication and Property of Water Level and Temperature Sensor for Medical Cooling System Using a Highly Sensitive GMR-SV Device (거대자기저항 스핀밸브 소자를 이용한 의료용 냉각기 수위 및 수온 센서의 제작과 특성)

  • Park, Kwang-Jun;Choi, Jong-Gu;Lee, Sang-Suk;Lee, Bum-Ju
    • Journal of the Korean Magnetics Society
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    • v.21 no.1
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    • pp.32-36
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    • 2011
  • We fabricated a sensor for measuring the water level and water temperature using GMR-SV (giant magnetoresistance-spin valve) device, simultaneously. It could be applied to the medical cooling system of the potassium titanylphosphate KTP) laser system for the therapy of a benign prostatic hyperplasia. The middle point of GMR-SV device was set to the near position of a high magnetic sensitivity with 2.8%/Oe. The sensitivity for the water level and water temperature of the fabricated sensor were $400\;m{\Omega}/mm$ and $100\;m{\Omega}/^{\circ}C$, respectively.

Electromechanical Properties of PMN-PT-PZ Composition for High Power Device (고출력 압전소자를 위한 압전 세라믹(PMN-PT-PZ)조성의 전기기계적 특성)

  • Lee, K.W.;Hong, J.K.;Jeong, S.H.;Lee, J.S.;Park, C.H.;Lim, K.J.
    • Proceedings of the KIEE Conference
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    • 1999.07d
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    • pp.1723-1725
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    • 1999
  • This paper is the study for piezoelectric properties of PMN-PT-PZ composition for high power piezoelectric device. It needs the properties such as high mechanical quality factor(Qm), high electromechanical coupling coefficient(kp) and high dielectric strain constant$(d_31)$, and the stable electromechanical properties under high vibration level. For acquiring this results, the value of x is changed in 0.1Pb$(Mn_{1/3}Nb_{2/3})O_3$+(0.9-x)$PbZrO_3+xPbTiO_3$ composition to find MPB(morphotropic phase boundary), and the piezoelectric constants is measured by resonance-antiresonance frequency method, based on IRE Standard. Also, it is measured as a function of the amount of additive, $Nb_2O_5$. When the composition is applied to high power device, the electromechanical properties is measured by laser vibrometer to confirm the reliablity under high vibration level. From these results, PMN-PT-PZ composition is shown excellent properties and capacity of application to high power device.

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Study on the variation of cellular physiology of Escherichia coli during high cell density cultivation using 2-dimensional gel electrophoresis

  • Yun, Sang-Seon;Lee, Sang-Yeop
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.219-222
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    • 2000
  • Physiological changes of Escherichia coli during the fed-batch fermentation process were characterized in this study. Overall cellular protein samples prepared at the different stage of fermentation were separated by 2-dimensional gel electrophoresis (2-DE), and differently expressed 15 proteins, Phosphotransferase enzyme I, GroEL, Trigger factor, ${\beta}$ subunit of ATP synthase, Transcriptional regulator KDGR, Phosphoglycerate mutase 1, Inorganic pyrophosphatase, Serine Hydroxymethyl-transferase, ${\alpha}$ subunit of RNA polymerase, Elongation factor Tu, Elongation factor Ts, Tyrosine-tRNA ligase, DnaK suppressor protein, Transcriptional elongation factor, 30S ribosomal protein S6 were identified using matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS). When bacterial cells grow to high cell density, and IPTG-inducible heterologous protein is produced, expression level of overall cellular proteins was decreased. According to their functions in the cell, identified proteins were classified into three groups, proteins involved in transport process, small-molecule metabolism, and synthesis and modification of macromolecules.

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Inverse Sta99ered Poly-Si TFT with a-Si Offset formed by Selective Excimer Laser Annealing (선택적 레이저 어닐링을 이용하여 비정질 실리콘 오프셋을 갖는 Inverse Staggered 다결정 실리콘 박막 트랜지스터)

  • Park, Kee-Chan;Choi, Kwon-Young;Kim, Cheon-Hong;Han, Min-Koo
    • Proceedings of the KIEE Conference
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    • 1997.07d
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    • pp.1633-1635
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    • 1997
  • For AMLCD pixel switching device, poly-Si TFT has the advantage of high field effect mobility over a-Si TFT. However, it also has some disadvantage such as large leakage current and more masking steps. We propose a new Inverse Staggered poly-Si TFT with a-Si offset. We have fabricated the new device and verified high ON/OFF current ratio. The device has lower leakage current level than the conventional Inverse Staggered poly-Si TFT and the same number of masking steps compared with conventional a-Si TFT's.

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Measurement of High Voltage Pulse using a KDP Pockels Cell (KDP Pockels 소자를 이용한 고전압 펄스 측정)

  • Cho, K.L.;Lee, J.H.;Chang, Y.M.;Koo, J.Y.;Kang, H.B.
    • Proceedings of the KIEE Conference
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    • 1993.07b
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    • pp.566-568
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    • 1993
  • A measuring technique for the fast transient high voltage is under development by use of laser source in order to substitute for the conventional measuring system which could bring about several technical inconveniences. At this moment, this work is aimed to construct a compact measuring system with the use of Pockels cell and specially designed voltage divider. This system is likely to enable us to measure the lightening impulse voltages at the level of 30kV, 50kV and 70kV. Throughout this work, qualified measuring devices are required to be developed for discriminating different kinds of noise from which the system should be protected.

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