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Characteristics and Antioxidant Properties of Yanggaeng Containing Enzymatic Hydrolyzed White Ginseng or Red Ginseng (효소 가수분해 백삼, 홍삼을 첨가한 양갱의 품질특성 및 항산화 활성)

  • Suh, Hee-Jae
    • Journal of Food Hygiene and Safety
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    • v.36 no.5
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    • pp.418-429
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    • 2021
  • This paper investigates the antioxidant activity and quality characteristics of yanggaeng containing white ginseng and red ginseng extracts and their enzyme hydrolysates that were produced for the purpose of the study. White and red ginseng extracts were hydrolyzed using Rapidase C80 max, Pyr-flo, and Ultimase MFC. Ginsenoside F2 and compound K (CK) were not detected in white and red ginseng before enzymic reaction but were detected in white and red ginseng hydrolyzed through Rapidase C80 max, Pyr-flo, and Ultimase MFC, and the content of CK was the highest in the second enzymic reaction group of red ginseng. Upon preparing yanggaeng containing white and red ginseng before or after enzymatic hydrolysis, the polyphenol content and antioxidant abilities were analyzed. The yanggaeng containing enzyme-hydrolyzed white ginseng and red ginseng showed greater total polyphenol content, superior DPPH radical scavenging activity, superior ABTS radical scavenging activity, and superior FRAP analysis results compared to the yanggaeng that doesn't contain white or red ginseng. As the enzymic reaction was performed in the added white and red ginseng, the antioxidant activity increased significantly (P<0.05). In brightness(L*), non-additive yanggaeng (control group) was the highest, red ginseng yanggaeng (RG) showed the highest redness(a*), and the white ginseng yanggaeng (WG) showed the highest yellowness(b*). In terms of texture, the yanggaeng containing red ginseng with second hydrolysis (RG-T2) showed significantly high results in hardness, springiness, chewiness, cohesiveness, and gumminess (P<0.05). In conclusion, treating white and red ginseng with Rapidase C80 max, Pyr-flo, and Ultimase MFC is very useful in ginsenoside deglycosylation and will produce CK with excellent biological activity. It can also be seen that yanggaeng containing white and red ginseng hydrolyzed with enzymes significantly increase total polyphenol and antioxidant activity compared to the control group (yanggaeng with no added ginseng). These results will be useful as excellent foundational data for the production of functional yanggaeng in the future.

Efficiency Comparison of Environmental DNA Metabarcoding of Freshwater Fishes according to Filters, Extraction Kits, Primer Sets and PCR Methods (분석조건별 담수어류의 환경 DNA 메타바코딩 효율 비교: 필터, 추출 키트, 프라이머 조합 및 PCR 방법)

  • Kim, Keun-Sik;Kim, Keun-Yong;Yoon, Ju-Duk
    • Korean Journal of Ecology and Environment
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    • v.54 no.3
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    • pp.199-208
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    • 2021
  • Environmental DNA (eDNA) metabarcoding is effective method with high detection sensitivity for evaluating fish biodiversity and detecting endangered fish from natural water samples. We compared the richness of operational taxonomic units(OTUs) and composition of freshwater fishes according to filters(cellulose nitrate filter vs. glass fiber filter), extraction kits(DNeasy2® Blood & Tissue Kit vs. DNeasy2® PowerWater Kit), primer sets (12S rDNA vs. 16S rDNA), and PCR methods (conventional PCR vs. touchdown PCR) to determine the optimal conditions for metabarcoding analysis of Korean freshwater fish. The glass fiber filter and DNeasy2® Blood & Tissue Kit combination showed the highest number of freshwater fish OTUs in both 12S and 16S rDNA. Among the four types, the primer sets only showed statistically significant difference in the average number of OTUs in class Actinopterygii (non-parametric Wilcoxon signed ranks test, p=0.005). However, there was no difference in the average number of OTUs in freshwater fish. The species composition also showed significant difference according to primer sets (PERMANOVA, Pseudo-F=6.9489, p=0.006), but no differences were observed in the other three types. The non-metric multidimensional scaling (NMDS) results revealed that species composition clustered together according to primer sets based on similarity of 65%; 16S rDNA primer set was mainly attributed to endangered species such as Microphysogobio koreensis and Pseudogobio brevicorpus. In contrast, the 12S rDNA primer set was mainly attributed to common species such as Zacco platypus and Coreoperca herzi. This study provides essential information on species diversity analysis using metabarcoding for environmental water samples obtained from rivers in Korea.

Improvement of Fluid Penetration Efficiency in Soil Using Plasma Blasting (플라즈마 발파를 이용한 토양 내 유체의 침투 효율 개선)

  • Baek, In-Joon;Jang, Hyun-Shic;Song, Jae-Yong;Lee, Geun-Chun;Jang, Bo-An
    • The Journal of Engineering Geology
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    • v.31 no.3
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    • pp.433-445
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    • 2021
  • Plasma blasting by high voltage arc discharge were performed in laboratory-scale soil samples to investigate the fluid penetration efficiency. A plasma blasting device with a large-capacity capacitor and columnar soil samples with a diameter of 80 cm and a height of 60 cm were prepared. Columnar soil samples consist of seven A-samples mixed with sand and silt by ratio of 7:3 and three B-samples by ratio of 9:1. When fluid was injected into A-sample by pressure without plasma blasting, fluid penetrated into soil only near around the borehole, and penetration area ratio was less than 5%. Fluid was injected by plasma blasting with three different discharge energies of 1 kJ, 4 kJ and 9 kJ. When plasma blasting was performed once in the A-samples, penetration area ratios of the fluid were 16-25%. Penetration area ratios were 30-48% when blastings were executed five times consecutively. The largest penetration area by plasma blasting was 9.6 times larger than that by fluid injection by pressure. This indicates that the higher discharge energy of plasma blasting and the more numbers of blasting are, the larger are fluid penetration areas. When five consecutive plasma blasting were carried out in B-sample, fluid penetration area ratios were 33-59%. Penetration areas into B-samples were 1.1-1.4 times larger than those in A-samples when test conditions were the same, indicating that the higher permeability of soil is, the larger is fluid penetration area. The fluid penetration radius was calculated to figure out fluid penetration volume. When the fluid was injected by pressure, the penetration radius was 9 cm. Whereas, the penetration radius was 27-30 cm when blasting were performed 5 times with energy of 9 kJ. The radius increased up to 333% by plasma blasting. All these results indicate that cleaning agent penetrates further and remediation efficiency of contaminated soil will be improved if plasma blasting technology is applied to in situ cleaning of contaminated soil with low permeability.

Cultivation Demonstration of Paprika (Capsicum annuum L.) Cultivars Using the Large Single-span Plastic Greenhouse to Overcome High Temperature in South Korea (고온기 대형 단동하우스를 이용한 파프리카 품종별 재배실증)

  • Yeo, Kyung-Hwan;Park, Seok Ho;Yu, In Ho;Lee, Hee Ju;Wi, Seung Hwan;Cho, Myeong Cheoul;Lee, Woo Moon;Huh, Yun Chan
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.429-440
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    • 2021
  • During the growing period, the integrated solar radiation inside the greenhouse was 12.7MJ·m-2d-1, and which was 90% of the average daily global radiation outside the greenhouse, 14.1MJ·m-2d-1. The 24-hour average temperature inside the greenhouse from July to August, which has the highest temperature of the year, was 3.04℃ lower than the outside temperature, and 4.07℃ lower after the rainy season. Before the operation of fog cooling system, the average daily RH (%) was lowered to a minimum of 40% (20% for daytime), making it inappropriate for paprika cultivation, but after the operation of fog system, the daily RH during the daytime increased to 70 to 85%. The average humidity deficit increased to a maximum of 12.7g/m3 before fog supply, but decreased to 3.7g/m3 between July and August after fog supply, and increased again after October. The daytime residual CO2 concentration inside the greenhouse was 707 ppm on average during the whole growing period. The marketable yield of paprika harvested from July 27th to November 23rd, 2020 was higher in 'DSP-7054' and 'Allrounder' with 14,255kg/10a and 14,161kg/10a, respectively, followed by 'K-Gloria orange', 'Volante' and 'Nagono'. There were significant differences between paprika cultivars in fruit length, fruit diameter, soluble solids (°Brix), and flash thickness (mm) of paprika produced in summer season at large single-span plastic greenhouse. The soluble solids content was higher in the orange cultivars 'DSP-7054' and 'Naarangi' and the flesh thickness was higher in the yellow and orange cultivars, with 'K-Gloria orange' and 'Allrounder' being the thickest. The marketable yield of paprika, which was treated with cooling and heating treatments in the root zone, increased by 16.1% in the entire cultivars compared to the untreated ones, increased by 16.5% in 'Nagano', 10.3% in the 'Allrounder', 20.2% in the 'Naarangi', and 17.3% in 'Raon red'.

Effects of Light Intensity on the Growth Performance, Blood Parameter and Immune Status of Broiler Chicks (조도가 육계 병아리의 생산성, 혈액성상 및 면역 수준에 미치는 영향)

  • Kim, Hee-Jin;Son, Jiseon;Jeon, Jin-Joo;Kim, Hyun-Soo;You, Are-Sun;Kang, Hwan-Ku;Kang, Bo-Seok;Hong, Eui-Chul
    • Korean Journal of Poultry Science
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    • v.48 no.3
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    • pp.143-150
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    • 2021
  • In this study, we investigated the effects of light intensity on broiler chick growth performance, blood parameters, and stress levels. A total of 240 one-day-old male Ross 308 broilers (47.97±0.166 g) were subjected to three different intensities of light (20, 30, and 50 lx), with each treatment being conducted with four replicates. On the seventh day, the growth performance (body weight, feed conversion ratio, and breast muscle and liver weights) and blood parameters were determined; the levels of serum corticosterone, interleukin-6 (IL-6), and tumor necrosis factor-α were also evaluated. The body weight, weight gain, liver weight, and breast muscle weight of chicks exposed to a light intensity of 50 lx were significantly increased compared with those of chicks subjected to 20 lx (P<0.05). No significant differences were observed in the leukocyte, erythrocyte, and platelet counts and the biochemical profile exceptions being the levels of glucose and inorganic phosphorus in the blood of the chicks in the three light intensity groups. However, serum corticosterone and IL-6 levels were the highest in chicks exposed to a light intensity of 20 lx (P<0.05). In conclusion, the findings of this study indicate that broiler chicks exposed to higher light intensity (50 lx) show significant improvements in terms of weight gain and corticosterone and IL-6 levels. Thus, high light intensities enhanced the growth performance, stress levels, and immune status of broiler chicks.

Inhibitory Effects of Cinnamon, Clove and Lemongrass Essential Oils against Biofilm Formation by Food Poisoning Bacteria (식중독 미생물의 biofilm 형성에 대한 계피, 정향 및 레몬그래스 정유의 억제 효과)

  • Kim, Hyeong-Eun;Kim, Yong-Suk
    • Journal of Food Hygiene and Safety
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    • v.36 no.5
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    • pp.430-439
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    • 2021
  • Essential oils with excellent antibacterial activity were used to study the inhibitory effect against the six types of food poisoning biofilms formed on the surfaces of polyethylene (PE) and stainless steel (SS) that are widely used for food processing instruments and containers. The antibacterial activity of 20 kinds of essential oils was tested using the disk diffusion method. The result showed the degree of antibacterial activity in the following order: cinnamon> clove> lemongrass> peppermint> pine needle (highest to lowest). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of cinnamon and clove oil were in the range of 0.63-1.25 mg/mL and 1.25-2.50 mg/mL, respectively. The MIC and MBC of lemongrass oil were 1.25-2.50 mg/mL and 2.50-5.00 mg/mL, respectively, showing slightly less antibacterial activity. Although the preventive effect of three types of essential oils on the biofilm formation differed slightly depending on food poisoning bacteria, PE, and SS, it was found that the precoating of 0.5% cinnamon, clove, and lemongrass oil on the PE and SS affects the formation of biofilm. Increased essential oil concentration significantly inhibited the biofilm formation for all food poisoning bacteria (P<0.05), and biofilms of Listeria monocytogenes and Staphylococcus aureus were not formed when treated with 0.5% cinnamon and clove oil. The elimination effect of food poisoning bacteria biofilms formed on the surfaces of PE and SS differed depending on the type of food poisoning bacteria. Still, the biofilm elimination effect increased as the essential oil concentration increased, and the biofilm elimination rate of clove oil was generally high. Therefore, this study found that the cinnamon and clove essential oils (0.5%) are suitable natural materials that effectively prevent, inhibit, and remove the biofilms formed by the food poisoning bacteria on the surfaces of polyethylene and stainless steel.

Experimental Study on the Performance Improvement of Velcro Reinforcement through Internal Filling (내부충진을 통한 벨크로 보강재의 성능향상에 대한 실험적 연구)

  • Jeong, Yeong-Seok;Kwon, Minho;Kim, Jin-Sup;Nam, Gwang-Sik
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.41 no.4
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    • pp.347-355
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    • 2021
  • During the earthquake, for multi-story structure, if the first floor is soft, the deformation will concentrate on that floor causing a serious damage to the column members which might leads to the collapse of the whole structure like Piloti structure during the Pohang earthquake in Korea. According to the 2016 National Disaster Management Research Institute's "Investigation of Seismic Reinforcement and Cost Analysis of Domestic Non-seismic Buildings", the rate of seismic resistance of private reinforced concrete buildings was 38.3 %. Among them, it was reported that the seismic-resistance ratio of the two to five-story structures was less than 50 %. Accordingly, the government is trying to improve the seismic rate through support projects, but the conventional seismic reinforcement methods are still expensive, and emergency construction is difficult. Therefore, in this study, the field applicability was evaluated by improving the reinforcement method using Velcro, which was developed through the research project of the Ministry of Land, Transport and Maritime Affairs in 2014. In order to improve the performance of the Velcro reinforcement method, introducing the initial tension of Velcro using high foaming rigid urethane filling between the Velcro and concrete of the columns was applied. Additionally, an experiment was conducted to evaluate the ductility of Velcro specimen from the concrete confinement effect. As a result, the ductility of the Velcro specimen was improved compare to Normal specimen. However, the energy dissipation capacity of VELCRO2 is better than VELCRO1, yet the maximum ductility of those two specimens did not show a significant difference. Therefore, the improvement of the internal filler material is still needed to have a better maximum ductility.

An Analysis of the Dynamics between Media Coverage and Stock Market on Digital New Deal Policy: Focusing on Companies Related to the Fourth Industrial Revolution (디지털 뉴딜 정책에 대한 언론 보도량과 주식 시장의 동태적 관계 분석: 4차산업혁명 관련 기업을 중심으로)

  • Sohn, Kwonsang;Kwon, Ohbyung
    • The Journal of Society for e-Business Studies
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    • v.26 no.3
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    • pp.33-53
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    • 2021
  • In the crossroads of social change caused by the spread of the Fourth Industrial Revolution and the prolonged COVID-19, the Korean government announced the Digital New Deal policy on July 14, 2020. The Digital New Deal policy's primary goal is to create new businesses by accelerating digital transformation in the public sector and industries around data, networks, and artificial intelligence technologies. However, in a rapidly changing social environment, information asymmetry of the future benefits of technology can cause differences in the public's ability to analyze the direction and effectiveness of policies, resulting in uncertainty about the practical effects of policies. On the other hand, the media leads the formation of discourse through communicators' role to disseminate government policies to the public and provides knowledge about specific issues through the news. In other words, as the media coverage of a particular policy increases, the issue concentration increases, which also affects public decision-making. Therefore, the purpose of this study is to verify the dynamic relationship between the media coverage and the stock market on the Korean government's digital New Deal policy using Granger causality, impulse response functions, and variance decomposition analysis. To this end, the daily stock turnover ratio, daily price-earnings ratio, and EWMA volatility of digital technology-based companies related to the digital new deal policy among KOSDAQ listed companies were set as variables. As a result, keyword search volume, daily stock turnover ratio, EWMA volatility have a bi-directional Granger causal relationship with media coverage. And an increase in media coverage has a high impact on keyword search volume on digital new deal policies. Also, the impulse response analysis on media coverage showed a sharp drop in EWMA volatility. The influence gradually increased over time and played a role in mitigating stock market volatility. Based on this study's findings, the amount of media coverage of digital new deals policy has a significant dynamic relationship with the stock market.

A Study on the Current Status of Ecological Restoration Plant Species Use - Focusing on the Ecosystem Conservation Cooperation Fund Return Projects - (생태복원 식물종 사용 실태에 관한 연구 - 생태계보전협력금 반환사업을 중심으로 -)

  • Cho, Dong-gil
    • Korean Journal of Environment and Ecology
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    • v.35 no.5
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    • pp.525-547
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    • 2021
  • The main purpose of this study is to examine the use of plant species in ecological restoration projects. To this end, planting drawings from 58 sites that had completed the return of the ecosystem conservation cooperation fund for the past six years were collected and analyzed. The analysis used the construction completion and design drawings to determine the overall selection status and analyze frequency by classifying planted plants into wild and cultivated plants by nature, size, vegetation climate, and upland and wetland habitat. The investigation and analysis process found many cases of wrong plant names, so an analysis was also performed on the matter. In the 58 investigation sites, 282 plants were used for planting: 91 tree species, 69 shrub species, 11 vine species, and 111 herbal species. The most commonly used plant species was Spiraea prunifolia f. simpliciflora, followed by Sorbus alnifolia, Quercus acutissima, Zoysia japonica, Callicarpa dichotoma, and Weigela subsessilisin that order. The most commonly used tree species was Sorbus alnifolia,followed by Quercus acutissima, Zelkova serrata, Chionanthus retusus, and Cornus officinalis, in that order. The most commonly used shrub species was Spiraea prunifolia f. simpliciflora, followed by Weigela subsessilis, Callicarpa dichotoma, Rhododendron yedoense f. poukhanense. and Euonymus alatusin that order. The most commonly used herbal plant species was Zoysia japonica, followed by Dendranthema zawadskii var. latilobum, Aster koraiensis, Miscanthus sacchariflorus, and Pennisetum alopecuroidesin that order. In the analysis by vegetation climate, Spiraea prunifolia f. simpliciflora, Callicarpa dichotoma, and Sorbus alnifoliawere most used in that order in both the temperate central and the warm temperate forest zones, but the pattern does not properly reflect the climate characteristics. In the analysis by habitat, Miscanthus sacchariflorus and Lythrum salicariawere most used in the wetland. In particular, the ratio of wild plants to cultivated plants was 76% to 24%, indicating the ratio of selecting cultivated plants was high. The names of plants on the drawings were mostly common names that did not appear in the Korea National Arboretum or the National Species List of Korea. It is necessary to use proper plant names in the future. Regarding the use of planting plants for ecological restoration, it is necessary to adopt the approach of diversifying selected plants, selecting plants according to characteristics of climate zones, and lowering the specifications of plants used for ecological restoration. Moreover, it is important to fully understand the ecological characteristics of wetland plants and minimize the ratio of using cultivated plants to ensure the plant selection centered on wild plants.

A Study on the Effect of Startup's Innovation Orientation on Growth Aspiration (창업기업의 혁신지향성이 성장열망에 미치는 영향에 관한 연구)

  • Oh, Hyemi;Lee, Chaewon;Kim, Jinsoo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.16 no.5
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    • pp.1-14
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    • 2021
  • Innovation and Scale-up of Start-up companies are becoming important national tasks. In the past, it was spread the start-up policy paradigm such as 'Start-up America', 'Start-up Chile', 'Start-up Britain' to overcome the recession globally. However as the economic recovery has become more visible recently in advanced economies, it is shifting from a start-up support policy to a scale-up oriented policy paradigm such as 'Scale-up America', Scale-up UK', 'Scale-up Denmark'. It is necessary to enter the scale-up phase beyond the start-up phase to increase the number of high-quality jobs and to continue economic growth. Therefore, it is necessary to grow the start-up into a strong medium-sized company and to lay the foundation for survival. Therefore, the purpose of this study is to consider the antecedent factors that influence the scale-up aspiration for the start-up firm to grow into a scale-up company, and empirically identifies the differences between the stages of economic development and entrepreneurs in the country. In order to accomplish the purpose, this study predicted scale-up by aspiration which is a predictor of scale-up behavior because it is difficult to achieve visible growth in a short period of time due to the characteristics of start-up companies. In order to empirically explore these relationships, the data were collected from nascent entrepreneurs who have less than 3.5 years of the Adult Population Survey(APS) among the subjects surveyed by the Global Entrepreneurship Monitor(GEM) and the national economic development stage are divided into Innovation-driven, Efficiency-driven, Factor-driven type economies. For the test hypotheses, this study adopted the multi-level model analysis for comparison between national economic development stages and using the R 3.5.0 program. The results of this study are as follows. There is difference between the national economic development and the entrepreneur in the relationship between innovation orientation of entrepreneurs and scale-up aspirations. As the economy of the country develops, the innovation activity of the entrepreneur becomes more active. Since start-ups are heavily influenced by entrepreneurs, there is a difference in the degree of aspiration depending on how innovative an entrepreneur is in the same environment. In terms of the relationship between innovation orientation and scale-up aspiration, the fear of failure was found to differ between national economic development and entrepreneurs. The fear of failure differ from country to country, and this is one of the important factors affecting entrepreneurial activities. It is expected that the factors influencing the growth of the start-up companies which are identified through the results of these studies, will be used to create a suitable scale-up ecosystem according to the national economic development stage.