• Korean Journal of Environmental Biology
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• v.17 no.1
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• pp.59-69
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• 1999

Bacterial population, signal compounds of pollution, bacterial productivity, heterotrophic activity and the activities of extracellular enzymes were analyzed four times from July 1994 to April 1995 at Daejin, Galnam, Pohang, and Ulsan in the coastal area of Bast Sea in order to investigate the state of eutrophication. The population of heavy metal resistant bacteria in Pohang was the highest among all stations. The bacterial populations which could utilize C -P compounds (phosphonate) showed the highest at Pohang that was expected for the industrial and agricultural effluents to inpour. It could be useful as an index of expectation about the influx of industrial sewages and agricultural effluents. The activities of extracellular enzymes for the degradation of organic matter were the highest in the Ulsan Chuyongam, but low in Daejin. These results showed positive relationship with the population of heterotrophic bacteria and bacterial productivity The ratio of poly-P and DNA in Pohang was the highest, and that in Ulsan, Galnam, and Daejin consecutively, corresponding with the distribution of heterotrophic bacteria. In case of the heterotrophic activities, each of the stations gives difference of seasonal variation in heterotrophic activities. Bacterial productivity seemed to be dependent on seasons; the highest in summer and the lowest in fall and winter. These seasonal variations of bacterial productivity seems to be similar with those of heterotrophic bacteria. It is suggested that the state of eutrophication could be evaluated by the criteria of the population sizes and the productivities of microorganisms, the amount of poly-P, heterotrophic activity, the activities of extracellular enzymes, etc.

• Journal of Korean Society of Water and Wastewater
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• v.22 no.2
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• pp.205-212
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• 2008

This study aimed to investigate the biofilm formation, bacterial regrowth, and bacterial community structure in the granular-activated carbon (GAC) filter adsorbers (FAs) used in water treatment plants. In 2005 and 2006, raw water, settled water, GAC FA by depth, and filtered water were collected twice a year from water treatment plants (WTPs) B and S. The number of heterotrophic bacteria, including mesophilic and psychrophilic bacteria, in such collected waters was investigated along with the total number of coliforms therein. Heterotrophic bacteria were detected in most samples, mainly at the surface layers of the GAC FAs, and fewer such bacteria were found in the lower and bottom layers. An increase in the bacterial number, however, was observed in the samples from various depths of the GAC FAs in WTPs B and S compared with the surface layers. An increase in the bacterial number was also detected in the filtered water. This may indicate that there is a regrowth of the bacteria in the GAC FA. Considering, however, that heterotrophic bacteria were not found in the filtered water, it can be deduced that most bacteria are removed in the chlorination process. Coliforms were detected at the surface layer of the GAC FAs, but their regrowth was not observed. MicroLog systems were used to identify the bacteria community distribution. Eight genera and 14 species, including Pseudomonas spp., were detected in WTP B, and 8 genera and 9 species, including Aeromonas spp., in WTP S. Further studies are required to elucidate their role in the biofilms in water treatment processes.

• Korean Journal of Microbiology
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• v.26 no.4
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• pp.332-338
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• 1988

The density of heterotrophic bacterial population and heterotrophic activity were measured at monthly interval from March, 1986 to March, 1987 at four sites in the Sudong Stream, a tributary of North Han River. Total bacterial numbers and maximum uptake velocity ($V_{max}$) of glucose ranged as 0.8-$25.2\times 10^{5}$ cells/ml, 0.0006-24.39.$\mu$gC/1/hr, respectively. $V_{max}$ of glucose showed marked seasonal periodicity, with highest values in summer. But density of viable bacteria varied considerably, with no definite seasonal pattern. At the nucontaminated site which located in upstream, heterotrophic bacterial population and activities were relatively low, and small variations were observed downstream flowing except the site where animal originated sewage inputs occurred. And this large input of allochthonous materials and bacteria was an important factor for the stream condition.

• Korean Journal of Microbiology
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• v.27 no.4
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• pp.378-384
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• 1989

The numerical taxonomy of heterotrophic bacterial community in Lake Soyang was analysed. 95, 115, 88 and 75 strains which were isolated at each season from spring in 1987 to winter in 1988 were clustered by single matching coefficient. The diversity indices (H') were in the range of 0.511-1.684, and the community was most diverse in spring. THe seasonal variation of generic composition was significant. Of the domonant genera, Acinetobacter, Pseudomonas, and Flavobacterium were representative.

• Korean Journal of Microbiology
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• v.49 no.4
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• pp.321-327
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• 2013

In the present work, the reactors that harbor bacterial biofilms including ammonia-oxidizing bacteria (AOB) and heterotrophic bacteria were treated with a continuous dose of chlorite ($0.66{\pm}0.01mg/L$) either with or without monochloramine at $1.77{\pm}0.03mg/L$. Both chlorite alone and combined chlorite-monochloramine applications effectively reduced biofilm and bulk AOB levels to near or below the detection limit ($0.6MPN/cm^2$ and 0.2 MPN/ml). The combined chlorite-monochloramine application exhibited greater AOB inactivation than chlorite alone. Unlike AOB, heterotrophic plate count (HPC) was unaffected by chlorite alone. In contrast to chlorite-only application, a combination of chlorite and monochloramine resulted in a significant reduction in HPC levels with log reductions of 3.1 and 3.0 for biofilm and bulk water, respectively. The results demonstrate that the combined chlorite-monochloramine application can provide an effective treatment for the inhibition of AOB and heterotrophic bacteria in a drinking water distribution system.

• Korean Journal of Microbiology
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• v.28 no.4
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• pp.331-336
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• 1990

Annual distribution of hiterotrophic marine bacteria and seasonal characteristics were investigated in the intertidal waters and sediments in the vicinity of Kunsan of Yellow Sea, Korea. Annual distribution of heterotrophic marine bacteria ranged from $ 7.5*10^{2}$ to $1.1*10^{5}$ c.f.u./ml in water and from $1.6*10 ^{4}$ to $4.8*10^{6}$ c.f.u.per g dry sediments. As for the morphological distribution measured by epifluorescence microscopy, rod-form bacteria were distributed more than 74% of total observed bacteria during surveying periods. Average biovolume of sampled bacteria ranged from $3.19\;+-\;0.59*10^{-2}$ to $6.19\;+-\;0.76*10^{-2}$ $\mu$$m^{3}$ for coccus-form bacteria. Isolated bacteria showed various utilization of carbon sources such as glucose, maltose, lactose, xylose and arabinose, and showed tolerance at various range of salinity. It was isolated 82 genus in seawater and 114 genus in sediments. Dominant genera were Pseudomonas, Vibrio, Flavobacterium and Acinetobacter in seawater, and Pseudomonas, Acinetobacter, Vibrio, and Mycobacterium in sediments.

• Journal of Korean Society on Water Environment
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• v.27 no.3
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• pp.334-341
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• 2011

The free residual chlorine of tap water samples, collected from 266 faucets on the water pipe network in Daegu City, was between 0.1 and 0.79 mg/L. On microorganic tests, general bacteria and the coliform goup were not detected and thus the tap water was turned out to be fit to drink. In particular, samples of which free residual chlorine was 0.1 mg/L and over were cultured in R2A agar media at $25^{\circ}C$ for 7 days, and as a result heterotrophic bacteria were detected in 65.9% of samples; (1). The closer tap water got to the faucet from the stilling basin, the lower residual chlorine concentration became but the more the bacterial count became. And, more bacteria were detected in the R2A agar medium than in the PCA medium. (2). In the case of separated strains, most colonies were reddish or yellowish. 16S rRNA sequence was identified as Methylobacterium sp. and Williamsia sp., and yellow strain was identified as Sphingomonas sp., Sphingobium sp., Novosphingobium sp., Blastomonas sp., Rhodococcus sp. and Microbacterium sp. White strain was identified as Staphylococcus sp. (3). Sterilized tap water in polyethylene bottles was inoculated with separated strain and was left as it was for 2 months. As a result, bio-film was observed in tap water inoculated with Methylobacterium sp. and Sphingomonas sp. It was found that heterotrophic bacteria increased when free residual chlorine was removed from tap water in the water pipe network. Thus, there is a need to determine a base value for heterotrophic bacteria in order to check the cleanliness of tap water in the water pipe network.

• The Korean Journal of Ecology
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• v.17 no.1
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• pp.79-90
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• 1994

Water samples were taken at 6 stations from the mouth of Keum River to Kogunsan Archipelago of West Sea during December, 1991 to August, 1992, to determine the distribution of heterotrophic bacteria, their biovolumes and heterotrophic activities. Heterotrophic marine bacteria ranged from $1.0\;{\times}\;10^3to\;5\;{\times}\;10^5c.f.u.$ /ml. As for morphological distribution measured by epifluorescence microscopy, rod-shaped bacteria were between 45% and 72% of all cells during investigation period. Average biovolume of sampled bacteria ranged from $(7.69\;{\pm}\;0.18)\;{\times}10^{-2}to\;(8.18\;{\pm}\;0.38)\;{\times}\;10^{-2}\;{\mu}m^3$ for coccoid bacteria, and from $(6.09\;{\pm}\;0.29)\;{\times}10^{-2}to\;(7.72\;{\pm}\;0.41)\;{\times}\;10^{-2}\;{\mu}m^3$ for rod-shaped ones. The activities of extracellular enzymes ranged from 0.01 to 2.6 ${\mu}M$ /l /hr for glucosidase, from 0.01 to 2.6 ${\mu}M$ /l /hr for amylase, from 0.01 to 8.86 ${\mu}M$ /I /hr phosphatase and from 0.01 to 0.94 ${\mu}M$ /l /hr for chitinase. Extracellular enzyme activities were higher in summer season than in other sampling periods, and phosphatase showed the highest activity among measured extracellular enzymes. Bacterial distribution and their extracellular enzyme activities were associated with water temperature and organic nutrients, but bacterial cell volumes showed no direct relationship with extracellular enzyme activities.

• Korean Journal of Microbiology
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• v.30 no.5
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• pp.383-390
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• 1992

In the periods of July 31 to August 10. 1988 and March 9 to 13. 1989. sediment samples were collected from the South Sea stations (010] to 092]) located in the area from $N 32^{\circ}$/30' to $34^{\circ}$/30', of latitude and from E $123^{\circ}$ 30' to $128^{\circ}$30' of longitude. These samples were analyzed for the number of total heterotrophic bacteria and extracellular digesting enzyme activities. In the 1989 spring period the number of heterotrophic bacteria in the sediment surface layer was increased more than 100 times at the maximum compared to that in the 1988 summer period. The proportion of fresh water bacteria to total heterotrophic bacteria was also higher in the spring period than the summer period. The extracellular digesting enzyme activities were higher in spring season than summer. Although the water content of sediment in the spring period was lower than that the summer period. the ash weight indicating organic material content was higher. These results means that the diameters of sediment particles were larger in spring than summer but the input of organic material into the sediment was greater. Based on these results bacterial distributions in the sediment layer of South Sea depend greatly on the season due to the effect of fresh water. During the spring season plankton could grow extensively owing to the inorganic nutrients input by the vertical mixing in the water column, then be precipitated into the sediment. Organic nutrients supplied from enzymatic degradation of polymeric particle from plankton can increase the bacterial number, too.

• KSBB Journal
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• v.29 no.1
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• pp.72-80
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• 2014

CW-4Y was identified as Stenotrophomonas sp. by morphological and physiological characteristics, and phylogenetic analysis of its 16S rDNA gene sequence. Nitrogen removal by CW-4Y was analyzed in relation to the ammonium concentration, presence of organic carbon, carbon source, and carbon-to-nitrogen ratio (C/N). Stenotrophomonas CW-4Y has heterotrophic nitrification and aerobic denitrification abilities. Stenotrophomonas CW-4Y utilized only glucose as carbon sources, and heterotrophic nitrification and aerobic denitrification were observed regardless of the type of nitrogen source. The maximum ammonium removal rate of CW-4Y was 80 $mg-N{\cdot}L^{-1}{\cdot}d^{-1}$ and its denitrification rate of 192 $mg-N{\cdot}L^{-1}{\cdot}d^{-1}$ at $NO_3{^-}-N$ (about 280 ppm) in shake culture experiments at a C/N ratio of about 15 was about 30 times higher than those of other bacteria with the same ability.