• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.3
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• pp.167-170
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• 2013

This study examined the growth performance and field evaluation of the dual herbicide-resistant transgenic creeping bentgrass plants. The effect of glyphosate treatment on the herbicide resistance of the transgenic creeping bentgrass plants was determined, and the non-transgenic control plant withered at the concentration $11{\mu}g/mL$ or higher whereas the transgenic creeping bentgrass plants survived the treatment at the concentration of $3,000{\mu}g/mL$, and the increase of the plant length was repressed as the glyphosate treatment concentration was increased. At field evaluation, glufosinate-ammonium and glyphosate were simultaneously treated to investigate the weed control effect. The results showed that more than 90% of the weeds withered four week after herbicide treatment, while the transgenic creeping bentgrass plants continued to grow normally. Therefore, the dual herbicide-resistant creeping bentgrass plants may be able to greatly contribute to the efficiency of weed control and to the economic feasibility of mowing in places such as golf courses.

• Korean Journal of Weed Science
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• v.16 no.1
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• pp.28-35
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• 1996

Tetrapion(2,2,3,3-tetrafluoropropionate sodium) has commonly been used for reed(Phragmites communis Trin.) control in uncultivated areas, especially in Japan. As an attempt to establish the screening system for selective herbicide controlling reed, tetrapion was tested as a standard herbicide to various weeds and crops including rice in a greenhouse. Symptoms of yellowing, twisting, stunting, and necrosis were observed in the herbicide-treated plants. The herbicide caused a severe damage on all crops examined, except cotton. Both direct seeded and transplanted rice were also sensitive to the herbicide. Its herbicidal activity was generally higher on grass weeds than on broadleaf weeds. It had a higher herbicidal activity with preemergence treatment than with postemergence treatment. In addition, field and greenhouse experiments were conducted to examine the effect of the herbicide on reed control. Its effect varied with the soil type of the treated field and the growth stage of reed. A relatively high dosage of the herbicide was required to control reed. It had an almost same effect on fairygrass(Miscanthus sinensis Anderss) as on reed. However, reed grown in a greenhouse was effectively controlled by the herbicide, having more effect with preemergence treatment than with postemergence treatment as observed in other plants treated with the herbicide. In conclusion, tetrapion has a great potential as a standard herbicide during the herbicide screening for selective reed control.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.3
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• pp.217-222
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• 1998

This experiment was carried out to investigate effects of herbicide application method on Calysteria jcqoonica control and corn yield at the forage experimental field, grassland and forage crops division, National Livestock Research Institute, RDA, Suwon from 1996 to 1997. The experiment was arranged in a randomized block design with three replication. The herbicide application consisted of control, 2~3 leaf stage(Dicamba), 5~6 leaf stage(Dicamba) and soil treatment (Pendimethalin). The hybrid of corn used in this experiment was P 3352. The results obtained are summarized as follow; 1. The plant height was the lowest at control and the highest at Dicamba treatment in 5~6 leaf stage, but no significant difference was found among herbicide application method at ear height. Tasseling and silking date were delayed 2~3 day at control. The length and weight of ear were highest at Dicamba treatment in 2~3 leaf stage. 2. The herbicide injury of corn was very slightly after 10-20 day, but corn was recovered soon. The weed control value was 94.2~67.5% at herbicide application plot. 3. The fresh and dry matter yield of Dicamba treatment in 2-3 leaf stage was highest as 42,878 and 16,033kg/ha, respectively. The application of Dicamba increased the DM yield of corn by 13~30%. 4. Herbicide application time had little effect on the ADF, NDF and CP contents, but the forage quality of ear was higher than that of stover. The result of this experiment indicate that the application of Dicamba in 2~6 leaf stage of corn will remove almost all of Calysreria jqonica and increase DM yield of com.

• Korean Journal of Plant Resources
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• v.17 no.1
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• pp.28-33
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• 2004

This study was conducted to simple transformants selection by herbicide Basta treatment after transformation with Agrobacteium tumefaciens MP90/PAT in hybrid poplar(Populus alba ${\times}$ P. glandulosa No. 3). In preliminary study, we determined that the lethal concentration of herbicide Basta was 1.0mg/L in callus culture, adventitious bud formation and axillary bud elongation experiment. By the treatment of 1.0mg/L Basta, we could be selected the transformed shoots effectively from the various cultures. In addition, the treatment was useful on selection of transformants which are growing in soil pot. Finally, we also confirmed the transformation by PAT assay, Above results show that the herbicide Basta treatment and PAT assay can be a very simple and effective method for the identification of PAT gene transformed hybrid poplar.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1473-1483
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• 2014

Reports of herbicide resistance events are proliferating worldwide, leading to new cultivation strategies using combinations of pre-emergence and post-emergence herbicides. We analyzed the impact during a one-year cultivation cycle of several herbicide combinations on the rhizobacterial community of glyphosate-tolerant Bt-maize and compared them to those of the untreated or glyphosate-treated soils. Samples were analyzed using pyrosequencing of the V6 hypervariable region of the 16S rRNA gene. The sequences obtained were subjected to taxonomic, taxonomy-independent, and phylogeny-based diversity studies, followed by a statistical analysis using principal components analysis and hierarchical clustering with jackknife statistical validation. The resilience of the microbial communities was analyzed by comparing their relative composition at the end of the cultivation cycle. The bacterial communites from soil subjected to a combined treatment with mesotrione plus s-metolachlor followed by glyphosate were not statistically different from those treated with glyphosate or the untreated ones. The use of acetochlor plus terbuthylazine followed by glyphosate, and the use of aclonifen plus isoxaflutole followed by mesotrione clearly affected the resilience of their corresponding bacterial communities. The treatment with pethoxamid followed by glyphosate resulted in an intermediate effect. The use of glyphosate alone seems to be the less aggressive one for bacterial communities. Should a combined treatment be needed, the combination of mesotrione and s-metolachlor shows the next best final resilience. Our results show the relevance of comparative rhizobacterial community studies when novel combined herbicide treatments are deemed necessary to control weed growth.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.2
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• pp.107-112
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• 1998

This experiment was carried out to investigate effects of herbicide application method on Abutilon avicennae control and corn yield at the forage experimental field, grassland and forage crop division, National Livestock Research Institute, RDA, Suwon from 1996 to 1997. The experiment was arranged in a randomized block design with three replication. The herbicide application consisted of control, 2-3 leaf stage(Dicamba), 5-6 leaf stage (Dicamba) and soil treatment(Pendimetha1in). The hybrid of corn used in this experiment was P 3352. The results obtained are summarized as follow; 1. The plant and ear height was the lowest at control. Tasseling and silking date were delayed 3-4 day at control. The length and weight of ear were the highest at Dicamba treatment in 2-3 leaf stage. 2. The herbicide injury of corn was detected slightly after 10-20 day but the injury was recovered soon. The weed control efficiency was 96.7 and 81.8% at Dicamba treatment in 2-3 and 5-6 leaf stage, respectively. 3. The fresh and dry matter yield of Dicamba treatment in 2-3 leaf stage was higher than that of control by 36%, but no significant difference was found among herbicide application method. 4. Herbicide application method had little effect on the ADF, NDF and CP contents, but the CP and IVDMD of ear were higher than that of stover. The results of this experiment indicate that the application of Dicarnba in 2-6 leaf stage of corn will remove almost all of A butilon avicennae and increase DM yield of corn.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.312-317
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• 2014

This study was conducted to obtain basis information for effective weed control by the fact-finding survey of herbicides use at farmer's level and distribution of herbicide resistant weeds in paddy field of Jeonbuk province, Korea. The distributions of major paddy field weeds and frequently used herbicide according to the survey were as follows. The dominant weeds in rice paddy field were Echinochloa crus-galli, Eleocharis kuroguwai, Scirpus juncoides, Monochoria vaginalis, etc.. Preferred herbicides widely used by farmers were ranked in the following order, early and middle treatment (48.0%), treatment before transplanting (36.7%) and foliar treatment (15.3%). The occurrence of herbicide-resistant weeds according to collected paddy soils was as follows. The occurring area of herbicide-resistant weeds was 24,413 ha, approximately 18.4% of rice cultivation area. Herbicide-resistant weeds were occurred in four species. The order of occurrence were Scirpus juncoides (39.0%) > Monochoria vaginalis (27.8%) > Echinochloa crus-galli (16.6%) and Cyperus difformis (16.6%). This information could be useful for estimation of future herbicide-resistant weed and establishment of herbicide-resistant weed control methods in Jeonbuk province, Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.3
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• pp.162-168
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• 1991

Iterbicide resduc. cauicd ~rljul-y to ovcrsown pasture grasses \\hen herbicide appliccl for use in pasturc est;~blish~nt~nt lo gro~vth contro! ol n;rtivc ~r ~:cds and shrubs. 'l'hih L~sperirnel~t was conducted to cv;lluati. (he herbicidr residue anti its ph!:totosical ~riiiuence on the growth of introtluccd pasturts species. Esperimcmtal soils was treated with buthitlazolc, ~ o d l ~ l m chlorate, glyphosatc. U-46 anti paraquat each at twt) applici~tion rates. Lhcf~1i.s glowl~mlc~ including othrr pasture grasses and le~urnes were evaluatrd for tolerance to herbicide. IIerbicide residue in the amount ot injury caused to introduced pasture grasses was found in order of buthidazole, sodium chlorate, U-46 and glyphosate. Euthidazole was not available for use in pasture establishment because of their long persist of toxic herbicide residues. A great stand redl~ction of subseq ~ ~ c n t oversown pastures was also observed In sodium chlorate treatment. However, normal vegetative stand and grass production was obtained, when thc pasture grasses seeded 45-60 days later herbicide treatments. Glyphosate did not affect subsequent oversown pasture species in both grasses and legumes. fistuccr arundinc~cea and Pou pratensis were the most tolerant pasture species to herbicide residue while 7'rzfi,lium pyatense and Medicugo suti~u showed a wsceptible response regardless of herbicide.

• Journal of The Korean Society of Clinical Toxicology
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• v.14 no.2
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• pp.71-77
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• 2016

Purpose: No studies have been conducted to investigate the acute toxicity of aryloxyphenoxypropionate herbicides in humans following ingestion. Therefore, this study was conducted to investigate the clinical characteristics of aryloxyphenoxypropionate herbicide poisoning and provide guidance for physicians treating patients who have ingested these types of herbicides. Methods: A retrospective observational case series was conducted using ten patients with history of aryloxyphenoxy propionate herbicide. Data were collected for clinical manifestation, management and final outcome. Results: The most common symptoms were gastrointestinal irritation and an altered mental state (Glasgow Coma Scale<15). An elevated lactate level was a common laboratory abnormality, and prolonged QTc interval was commonly observed. These clinical features normalized within one day of supportive treatment. Conclusion: The acute toxicity of aryloxyphenoxypropionate herbicides in humans is manageable with supportive treatment. However, physicians should take into account depressed consciousness, the possibility of arrhythmia, and an elevated lactate level when planning their treatment strategy.

• Journal of Applied Biological Chemistry
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• v.48 no.4
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• pp.187-192
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• 2005

Influence of herbicide oxadiazon on soil microbial activity and nitrogen dynamics was evaluated. Soil samples were treated with oxadiazon at field and tenfold field rates and incubated. Organic amendment was added as an additional substrate for soil microorganisms. Tenfold field rate oxadiazon stimulated substrate-induced respiration (SIR) and dehydrogenase activity (DHA) in amended soil as compared to unamended soil and control treatment. Soil urease activity was not affected by oxadiazon treatment. In both amended and unamended soils, treatment of the herbicide at higher rate had not significant influence on $NH_4$-N and $NO_3$-N concentrations. Higher dose of oxadiazon was degraded in both soils, but dissipation rate in amended soil was higher than unamended soil, with half-lives ($t_{1/2}$) of 23.1 and 138.6 days, respectively. Recommended field rate did not affect microbial activity and nitrogen dynamics in soil ecosystem. Results showed influence of oxadiazon on cycling processes of nitrogen in soil was not significant however its effect on microbial activity was a tendency depending on addition of organic amendment to soil.