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An Effective Selection of PAT Gene Transformed Populus alba $\{times}$ Populus glandulosa No.3 using Herbicide Basta Treatment  

오경은 (충북대학교 산림과학부, 임업연구원 임목육종부)
문흥규 (충북대학교 산림과학부, 임업연구원 임목육종부)
박재인 (경희대학교 생명과학대학 및 한방재료가공연구센타)
양덕춘 (경희대학교 생명과학대학 및 한방재료가공연구센타)
Publication Information
Korean Journal of Plant Resources / v.17, no.1, 2004 , pp. 28-33 More about this Journal
Abstract
This study was conducted to simple transformants selection by herbicide Basta treatment after transformation with Agrobacteium tumefaciens MP90/PAT in hybrid poplar(Populus alba ${\times}$ P. glandulosa No. 3). In preliminary study, we determined that the lethal concentration of herbicide Basta was 1.0mg/L in callus culture, adventitious bud formation and axillary bud elongation experiment. By the treatment of 1.0mg/L Basta, we could be selected the transformed shoots effectively from the various cultures. In addition, the treatment was useful on selection of transformants which are growing in soil pot. Finally, we also confirmed the transformation by PAT assay, Above results show that the herbicide Basta treatment and PAT assay can be a very simple and effective method for the identification of PAT gene transformed hybrid poplar.
Keywords
Hybrid poplar herbicide resistant gene; simple selection of transformants; herbicide Basta; PAT assay;
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Times Cited By KSCI : 2  (Citation Analysis)
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