• 대한한의학회지
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• 제18권2호
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• pp.187-198
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• 1997

The degree of KAP study on Chinease traditional medicine and doctor was examined with some chinease living yenbian district(延邊) from July 1st to August 30th in 1996. The result of the study for predicting health and ill patterns runs as fellows. 1. It is understand to strength and variety about role of chinease traditional medicine doctors and the curable disease. 2. Although most patients go to chinease traditional medicine clinic for the purpose of medicine herbs in package, acupuncture and industry-made pharm, they think the medical fee a little high(70.6%). 3. The 66.7% of respondents recognize chinease traditional medicine doctors as a profession and others think them only abundant works ; therefore it is rather low to ink chinease traditional doctors a profession. 4. Most respondents think that chinease traditional medicine should improved in the inside ; such as scientific reinforcement of theory, lack of univerality, improvment and enlargement of insurance, unkindness, shortage of publicity, dropped equipment, system of medical specialist, lack of integration with westem medicine, exact diagnosis and confidence of remedy, and low efficacy, etc. 5. Chinease likes more experienced-traditional doctor than beginner(78.3%) 6. The policy of korean government against 100 herbal prescription right by western pharmacy has taken the negative recognition(74.6%), 7. The degree that acknowleges of chinease traditional medicine through thease basic contents is average 47.3 mark. To be brief, although the step of the recognition and attitude of oriental medicine is less or very affirmative aspect, actully considerable positive factors is in the last chosen step. In conclusion KAP study connected chinease traditional medicine and doctor has very much postive factors.

• 대한한의학방제학회지
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• 제26권3호
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• pp.223-236
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• 2018

Objectives : We compared the inhibitory effects of herb-combined remedies, which were recorded on "Yooam" prescription of Dongeuibogam, on cell migration and invasion, two critical cellular processes that are often deregulated during metastasis, in B16F10 melanoma cells. For this purpose, water extracts of Sipyukmiryukieum (SYMRKU), Danjacheongpitang (DJCPT), Cheongganhaeultang (CGHUT) and Jipaesan (JPS) were used. Methods : Cytotoxicity was assessed by an MTT assay. Wound healing and matrigel transwell assays were used to examine on B16F10 cell migration and invasion. The levels of mRNA and protein expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) were analyzed by RT-PCR and Western blotting. Results : Our data showed that DJCPT showed the strongest inhibitory effect among the four prescriptions in inhibiting cell motility of B16F10 melanoma cells within the concentration range that was not cytotoxic. The inhibitory potential of colony formation was higher in DJCPT and SYMRKU compared to the other two types of prescriptions, and the inhibitory effect of invasiveness is shown in order of DJCPT, SYMRKU, CGHUT and JPS. DJCPT, and SYMRKU strongly inhibited the activity and expression of MMP-2 and MMP-9, which are important mediators in cancer invasion, compared to CGHUT and JPS, and the increased expression of TIMP-1 and TIMP-2 was also more effective in these two prescriptions. In conclusion, DJCPT is expected to exhibit the most potent blocking effect on migration and invasion among four herb-combined remedies compared in B16F10 melanoma cells. Conclusion : Overall, the results of this study will be used as an important source to validate these prescriptions in animal models and to understand the mechanism of action of herbal remedies recorded in Dongeuibogam.

• 동의신경정신과학회지
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• 제31권1호
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• pp.49-61
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• 2020

Objectives: The objective of this study is to analyze clinical reports of treatment of menopausal depression in traditional Chinese medicine (TCM). Methods: This study included randomized controlled trials (RCT) in treating menopausal depression. Literature studies of Chinese National Knowledge Infrastructure Database(CNKI) were performed. Clinical reports on menopausal depression treatment were searched using the following key words in the CNKI: 'menopausal depression' and 'postmenopausal depression'. Results and Conclusions: A total of 24 RCT articles on menopausal depression were selected. The most widely used diagnostic tool for menopausal depression was CCMD-3. Effective rate and HAMD were the most commonly used assessment tools. Bosinhaeultang-gagam (Bushenjieyu-tang jiajian) and Gami-gammaegdaejotang(Jiawei ganmaidazao-tang) were used much more than other prescriptions, while Bupleuri Radix was the most used single medicine herb. Additionally, the Chinese medicine treatment group showed less adverse reactions than the control group. As a result, we could know frequently used herbal prescription and single medicinal herbs and this can be helpful in developing more advanced studies in future.

• 대한한의학회지
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• 제32권5호
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• pp.12-24
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• 2011

Objectives: This study analyzed literature published in Korea using Tae-eumin prescriptions and herbs for obesity for future research. Methods: We conducted a computerized search at journal web sites and manual searches. A total of 25 sites were included. Obesity, obese, over weight, herb, herbal, taeum, taeyuem, and taeeum were selected keywords and they were combined properly. Results:A total of 28 studies were analyzed. The percentage of clinical and case report studies was 35.7% and experimental studies 64.3%. The most frequently used prescription was Taeeumjowui-tang (太陰調胃湯) and herbs were Raphani semen(蘿葍子) and Platycodon grandiflorum(桔梗). Weight (kg), body mass index (BMI) and side effects and symptoms were used as indices in clinical and case report studies. Weight, weight gain percentage and blood serum lipid profiles were frequently used as indices in experimental studies. Conclusion: Further well-designed randomized controlled studies and clinical reports of side effects and symptoms are needed for development of obesity studies for Tae-eumin prescriptions.

• 동의생리병리학회지
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• 제21권6호
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• pp.1555-1563
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• 2007

To idenifty effect of Bojungbangam-tang kakambang on Cisplatin-Induced G2/M Phase Arrest in Human Renal Proximal Tubular HK-2 Cells. Cytotoxicity of cisplatin was detected in HK-2 cells and the value of IC50 is about $25\;{\mu}M$. The treatment of cisplatin to HK-2 showed the G2/M phase cell cycle arrest. The ethanol extract of Bojungbangam-tang kakambang (EBTKB), a new herbal prescription composed of ten crude herbs, inhibited cisplatin-induced G2/M phase arrest in HK-2 cells. EBTKB increased G0/G1 peak in cisplatin-treated HK-2 cells. p53, p21 and p27 expression were increased in cisplatin-treated HK-2 cells. Inhibitory effect of EBTKB on cisplatin-induced G2/M phase arrest was accomplished through inhibition of p53, p21 and p27 expression. Also, reduced CDK2 and cyclin A expression by cisplatin were increased by EBTKB, but cyclin E was not changed. Reduction of ERK activation and increment of p38 activation by cisplatin were increased ERK activation and decreased p38 activation by EBTKB. Cisplatin had no effect on JNK activation, but EBTKB increased JNK activation. These results can suggest that EBTKB inhibits cisplatin-induced G2/M phase arrest in HK-2 cell through reduction of p53-dependent p21 and p27 protein, ERK activation and p38 inactivation.

• 대한한의학방제학회지
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• 제16권1호
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• pp.117-130
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• 2008

Korean traditional medicine has been used for the treatment of the various diseases based on both oriental medicinal theory and clinical trials. Thus, the prescriptions of Korean traditional medicine would be useful for the development of new therapeutics. This research focuses on the fundamental study in Korean traditional prescriptions for the development of new hepatoprotective agents. We found two prescriptions. Injinho-Tang and Osumogwa-Tang, showed the significant DPPH free radical scavenging and hepatoprotective effect, respectively. It is well-known that free radical scavenging effect is related to the prevention of various pathological events including liver injury. This paper deals with hepatoprotective effects on tacrine-induced cytotoxicity in Hep G2 cells, free radicals scavenging on both DPPH and superoxide of above two prescriptions. Hot water extract of Injinho-Tang did not show the significant hepatoprotective effect on tacrine-induced cytotoxicity in Hep G2 cells, however, it shows the significant scavenging effects for both DPPH and superoxide radicals. On the other hand, all of the hot water extracts of constituent herbal drugs in Injinho-Tang exhibited the promising protective effect on tacrine-induced cytotoxicity in Hep G2 cells. Of these, water extract of Rhei Rhizoma showed the most prominent effect on tacrine-induced cytotoxicity in Hep G2 cells. Bioassay-guided fractionation of Rhei Rhizoma extract has furnished four compounds, and their chemical structures have been identified by comparison of their spectral data with those of literature as chrysophanol (1), emodin (2), 3,5-dihydroxy-4'- methoxystilbene (3), and rhapontigenin (4), respectively. Among the isolated compounds, compounds 2-4 revealed the significant hepatoprotective effect in vitro when their $EC_{50}$ values compare with that of silybin, as a positive control. It also exhibited that emodin possessed the most hepatoprotective effect among these active compounds. In case of Osumogwa-Tang, its hot water extract showed the moderate protective effect on tacrine-induced cytotoxicity in Hep G2 cells. Hot water extract of Chaenomelis Fructus, one of the constituent herbal drug of this prescription, exhibited the significant hepatoprotective effect with $EC_{50}$ value of $7.8{\pm}0.1\;{\mu}g/ml$, however, it showed strong cytotoxicity in Hep G2 cells above the concentration of $25\;{\mu}g/ml$. It was revealed that both hot water extract of Evodiae Fructus and its butanol soluble fraction showed the moderate hepatoprotective effect but concentration-dependent activity in Hep G2 assay system. Two quinolone alkaloids, evocarpine and dihydroevocarpine, also tested for their hepatoprotective effects on tacrine-induced cytotoxicity in Hep G2 cells, however, these two compounds derived from the Evodiae Fructus as the major constituents did not show in vitro hepatoprotective effect. From these results, it would be necessary to further isolation of its hepatoprotective compounds from the butanol soluble fraction of the hot water extract of Evodiae Fructus.

• 한국산학기술학회논문지
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• 제15권7호
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• pp.4682-4691
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• 2014

반하백출천마탕은 다양한 약리활성을 가진 전통한방처방이다. 본 연구는 한방처방의 품질관리를 위한 기초자료로서, 고성능액체 크로마토그래피 (RP-HPLC)을 이용한 반하백출천마탕에서 12종 지표성분 Homogentisic acid, Hesperidin, Naringin, Alisol A, Atractylenolide II, Atractylenolide III, Ginsenoside Rg1, Formononetin, Gastrodin, Berberine, Palmatine, 6-gingerol를 동시분석 하였다. 표준액은 상업용 $C_{18}$ 역상 컬럼과 이동상으로 물(0.1% TFA)과 아세토나이트릴을 사용하여 기울기용매조성법인 유속은 1.0mL/min, 주입부피는 $10{\mu}L$, 컬럼오븐 온도 $40^{\circ}C$, 파장 200, 220, 280, 340nm에서 수행되었다. 이 결과 모든 표준성분의 검량선은 우수한 직선성 $r^2$ >0.999으로 나타내었다. 또한 검출한계(LOD)와 정량한계(LOQ)는 $0.012{\sim}0.878{\mu}g/mL$ 및 $0.009{\sim}0.290{\mu}g/mL$, 정밀성 intra-day와 inter-day는 0.07~1.21%및 0.20~0.90%, 회수율은 97.17~108.40%, 각각의 함량은 매우 미미한 변화를 타나내었다. 이 연구결과는 실험적으로 입증된 동시분석법의 확립에 따라 반하백출천마탕의 품질평가를 효율적으로 보여주었다.

• 동국한의학연구소논문집
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• 제11권
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• pp.1-40
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• 2008

The leukemia is caused by with abnormal changes of the hemotopoietic cell and in the various neoplasm group which occurs from lymph organization and the bone marrow occurs disrupts immigrates toward the blood of circumference and a hemotopoiesis and the arm discouragement royal tomb and is a disease which infiltrates with the organization. Western medical diagnosis and classification and presents eastern medicinal demonstration which is confronted in him about the leukemia, eastern medicinal diagnosis and treatment the clinical dissertation against precisely, saw and dissertation precisely tried about leukemia therapeutic effect of the herbal extract, last from conclusion like this dissertation precisely in the base which tries groped the direction of eastern medicinal treatment method research about the leukemia. Eastern medicine approach led about diagnosis and treatment of the leukemia and with leukemia treatment the medical herb which the eastern medicine possesses in development and room sacrifice an eastern medicine theory and the false eye presented the drug which is therapeutic possibility and they presented. The eastern medicine demonstration and therapeutic method is showing the therapeutic ratio which is effective about the leukemia. But according to the patient does a about prescription and therapeutic method differently and the room my composition is complicated and clinical day is consistent decides upon a reproducibility and a therapeutic ratio with difficult point of accumulation, with standardization of therapeutic method has the difficult point of supply. In treatment of the leukemia demonstrates a nature of a disease consequently with theory of eastern medicine and appropriately prescribes endeavors raises a therapeutic ratio and the medical herb and room proposal remedial value, under verifying reveals the clinical trial which is standardized leads and the medical herb and a room sacrifice and the verification and supply of effect and safety are necessary.

• 동의신경정신과학회지
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• 제19권3호
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• pp.195-203
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• 2008

Objective: 'GyoGam-Dan' is known as a medicine to cure depression in Donguibogam (traditional Korean oriental medicine book). GyoGam-dan which is composed of Cyperi Rhizoma L. and Poria cum Radix pini has been used by basic prescription of asymptom related to all kinds of Qi(氣) disease. Although it has been clinically used for a long time, its safety have never been proved till now. Methods: We had collected data of 610 cases of patients taking 'GyoGam-Dan' at Wonkwang univ. oriental medical center for a period of three years (2005.3.09-2008.04.30). On the patients' blood, the levels of LFT and RFT were examined and the residual levels of heavy metals and pesticides were measured. Results: There were 610 patients who had taken GyoGam-Dan for about three years. In the patients, the most common diseases is depression, followed by palpitation, and then insomnia. Among the patients' estimations on its effectiveness, there were many of positive ones. The blood analyses of patients who had taken GyoGam-Dan did not show any problems and moreover there was not any problems in the levels of residual heavy metals and agricultural pesticides. Taken together, GyoGam-Dan was proved to be a safe medicine for health. Conclusions: Our clinical and chemical studies show that GyoGam-dan has the anti-stress effect and is a safe medicine of which side effect does not exist. Hereafter, Nigorous clinical trial on GyoGam-dan is required.

• 대한한의학회지
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• 제39권3호
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• pp.17-27
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• 2018

Objectives: Ukgan-san does not appear in Traditional Korean Medicine classics. However, it has been used in research and clinical applications in Korea. In this paper, we are aiming to examine the records concerned with Ukgan-san to the present, and announce the present usage state in Korea. Methods: We searched and summarized the contents of previous medical books about Ukgan-san. In order to investigate the current status of utilization of Ukgan-san in Korea, a bibliographic database of Korea, a database of clinical researches, and a database of pharmaceuticals of the Korea Food and Drug Administration were searched. Results: As a result of searching Ukgan-san related documents, Ukgan-san has been developed in China (Ming dynasty). However, its clinical application has been expanded by modern Japanese kampo medicine doctors. In Korea, cases of Parkinson's disease, other movement disorders, and psychiatric disorders have been reported, and randomized controlled trials had been done and planned. In addition, some extracts which are consisted of Ukgan-san are also available. Conclusions: In Korea, Ukgan-san has been influenced by Japan and has been used for clinical and research purposes. The utilization rate in Korea is still small, but the utilization rate will increase according to the results of clinical studies.