Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effect of Bojungbangam-tang Kakambang on Cisplatin-Induced G2/M Phase Arrest in Human Renal Proximal Tubular HK-2 Cells

보정방암탕가감방(保正防癌湯加減方)이 cisplatin으로 유도된 인간 근위세뇨관 HK-2세포의 G2/M phase arrest에 미치는 영향

  • Park, Sung-Cheul (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Su-Kyung (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Yeom, Seung-Ryong (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Kwon, Young-Dal (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Song, Yung-Sun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University)
  • 박성철 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이수경 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 염승룡 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 권영달 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학과교실)
  • Published : 2007.12.25
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Abstract

To idenifty effect of Bojungbangam-tang kakambang on Cisplatin-Induced G2/M Phase Arrest in Human Renal Proximal Tubular HK-2 Cells. Cytotoxicity of cisplatin was detected in HK-2 cells and the value of IC50 is about $25\;{\mu}M$. The treatment of cisplatin to HK-2 showed the G2/M phase cell cycle arrest. The ethanol extract of Bojungbangam-tang kakambang (EBTKB), a new herbal prescription composed of ten crude herbs, inhibited cisplatin-induced G2/M phase arrest in HK-2 cells. EBTKB increased G0/G1 peak in cisplatin-treated HK-2 cells. p53, p21 and p27 expression were increased in cisplatin-treated HK-2 cells. Inhibitory effect of EBTKB on cisplatin-induced G2/M phase arrest was accomplished through inhibition of p53, p21 and p27 expression. Also, reduced CDK2 and cyclin A expression by cisplatin were increased by EBTKB, but cyclin E was not changed. Reduction of ERK activation and increment of p38 activation by cisplatin were increased ERK activation and decreased p38 activation by EBTKB. Cisplatin had no effect on JNK activation, but EBTKB increased JNK activation. These results can suggest that EBTKB inhibits cisplatin-induced G2/M phase arrest in HK-2 cell through reduction of p53-dependent p21 and p27 protein, ERK activation and p38 inactivation.

Keywords

References

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