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http://dx.doi.org/10.5762/KAIS.2014.15.7.4682

Simultaneous analysis of 12 kind maker components in Banhabaekchulchhonma-tang by RP-HPLC  

Lee, Kwang Jin (Korea Institute of Oriental Medicine(KIOM), KM-Based Herbal Drug Development Group)
Yang, Hye Jin (Korea Institute of Oriental Medicine(KIOM), KM-Based Herbal Drug Development Group)
Ma, Jin Yeul (Korea Institute of Oriental Medicine(KIOM), KM-Based Herbal Drug Development Group)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.15, no.7, 2014 , pp. 4682-4691 More about this Journal
Abstract
Banhabaekchulchhonma-tang is a traditional Korean herbal prescription with a range of pharmacological activities. In this study, the simultaneous analysis of 12 kinds of marker components, Homogentisic acid, Hesperidin, Naringin, Alisol A, Atractylenolide II, Atractylenolide III, Ginsenoside Rg1, Formononetin, Gastrodin, Berberine, Palmatine and 6-gingerol, in Banhabaekchulchhonma-tang was performed using high-performance liquid chromatography (RP-HPLC). The standard sample of commercial $C_{18}$ reversed phase-column using water (0.1% TFA) and acetonitrile as the mobile phase with a step gradient elution mode. The flow rate (1.0mL/min), injection volume ($10{\mu}L$) and column oven temperature ($40^{\circ}C$) at a 200, 220, 280 and 340nm wavelength was conducted. All calibration curves of the standard components showed good linearity ($r^2$ >0.999). In addition, the limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.012 to $0.878{\mu}g/mL$ and 0.009 to $0.290{\mu}g/mL$. The precision intra-day and inter-day were ranged from 0.07 to 1.21% and 0.20 to 0.90%, respectively. The recoveries ranged from 97.17 to 108.40%. Each sample amount showed a very small change. These results highlight the efficient quality evaluation of Banhabaekchulchhonma-tang.
Keywords
Banhabaekchulchhonma-tang; Medicinal herbs; RP-HPLC; Simultaneous analysis;
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Times Cited By KSCI : 8  (Citation Analysis)
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