• 제목/요약/키워드: Herbal crops

검색결과 203건 처리시간 0.023초

2007-2011 우리나라 농업현장 임상진단 요청 작물의 바이러스 감염 특성 (2007-2011 Characteristics of Plant Virus Infections on Crop Samples Submitted from Agricultural Places)

  • 김정수;이수헌;최홍수;김미경;곽해련;김정선;남문;조점덕;조인숙;최국선
    • 식물병연구
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    • 제18권4호
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    • pp.277-289
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    • 2012
  • 농업인 등 농업현장에서 임상진단을 요청한 시료 수는 2007년부터 5년간 총 2,992건이었으며, 바이러스를 검정한 샘플 수는 13,325개이었다. 연도별 검출된 바이러스 종류는 2007년 21종, 2008년 15종, 2009년 23종, 2010년 21종, 2011년 17종이었다. 5년 동안에 우리나라에서 처음 검정된 바이러스는 2007년에는 TbLCV, 2008년에는 TYLCV, 2009년에는 INSV, RaMV, 2010년에는 BWYV이었다. 2007년부터 5년간 총 발생한 바이러스 종류는 알파파모자이크바이러스(Alfalfa mosaic virus, AMV) 등 총 40종이었다. 임상진단 요청 시료에서 가장 감염율이 높은 상위 10종의 바이러스는 CMV, TSWV, TYLCV, CGMMV, BBWV2, ZYMV, MNSV, PMMoV, WMV, PepMoV이었다. 농업현장에서 임상진단 요청한 작물의 종류는 총 53종이었으며, 상위 10위의 작물 종류는 고추, 토마토, 파프리카, 수박, 멜론, 벼, 오이, 옥수수, 무, 박이었으며, 상위 10개 작물과 20개 작물의 전체 임상진단 요청률은 각각 81.6%와 94.2%이었다. 고추에 감염된 바이러스는 BBWV2 등 8종이었으며, 검정한 샘플 가운데 바이러스가 검출된 비율은 CMV 24.6%, PMMoV 18.9%, TSWV 14.7%이었다. 2종 바이러스 복합감염 형태는 평균 감염률 14.7%의 BBWV2+CMV 등 7가지, 3종 바이러스 복합감염 형태는 평균 감염률 0.9%의 BBWV2+CMV+PepMoV 등 4가지이었다. 토마토에서 검출된 바이러스 종류는 CMV 등 6종이었으며 검정한 샘플 중 바이러스가 검출된 비율은 TYLCV 50.6%, TSWV 14.5%, TbLCV 10.9%이었다. 복합감염형태와 감염률은 CMV+TSWV 3.9%, ToMV+TYLCV 0.4%이었다. 수박에 감염된 바이러스는 CMV 등 5종이었으며 평균 검출 비율은 MNSV 37.0%, CGMMV 20.4%, ZYMV 18.1%, WMV 17.8%이었다. 수박 바이러스의 복합감염 형태와 검출비율은 CMV+MNSV 0.7%, WMV+ZYMV 5.0%이었다. 멜론에서 검출된 감염된 바이러스는 CMV 등 3종이었고 평균 검출비율은 MNSV 77.6%, CMV 5.6%, WMV 3.3%이었다. 복합감염은 CMV+ MNSV로 평균 검출비율은 13.5%이었다.

Inhibitory Effect of Chitosan and Phosphate Cross-linked Chitosan against Cucumber Mosaic Virus and Pepper Mild Mottle Virus

  • Gangireddygari, Venkata Subba Reddy;Chung, Bong Nam;Cho, In-Sook;Yoon, Ju-Yeon
    • The Plant Pathology Journal
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    • 제37권6호
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    • pp.632-640
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    • 2021
  • Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material - chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

Development of a SCAR Marker Linked to Ph-3 in Solanum ssp.

  • Park, Pue Hee;Chae, Young;Kim, Hyun-Ran;Chung, Kyeong-Ho;Oh, Dae-Geun;Kim, Ki-Taek
    • 한국육종학회지
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    • 제42권2호
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    • pp.139-143
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    • 2010
  • Late blight caused by Phytophthora infestans is historically a serious epidemic disease in potato and tomato cultivations. Accession L3708 (Solanum pimpinellifolium), a new source for late blight resistance was identified in AVRDC, and carries the resistance gene, Ph-3, incompatible to P. infestans race 3. The AFLP markers linked to Ph-3 were previously developed from the L3708 accession (Chunwongse et al. 2002). To facilitate tomato breeding with the Ph-3 gene, an attempt was made to convert AFLP markers to sequence-characterized amplified region (SCAR) markers. Among 6 AFLP markers, only one AFLP marker, L87, was successfully converted to SCAR marker. The resistance-specific 230 bp AFLP fragment was cloned and sequenced, and the PCR primer amplifying a 123 bp fragment was designed. This SCAR marker could discriminate resistant and susceptible individuals with high stringency. The developed SCAR marker could be used for the marker assisted-selection in tomato breeding programs.

Viral Metatranscriptomic Analysis to Reveal the Diversity of Viruses Infecting Satsuma Mandarin (Citrus unshiu) in Korea

  • Hae-Jun Kim;Se-Ryung Choi;In-Sook Cho;Rae-Dong Jeong
    • The Plant Pathology Journal
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    • 제40권2호
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    • pp.115-124
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    • 2024
  • Citrus cultivation plays a pivotal role, making a significant contribution to global fruit production and dietary consumption. Accurate identification of viral pathogens is imperative for the effective management of plant viral disease in citrus crops. High-throughput sequencing serves as an alternative approach, enabling comprehensive pathogen identification on a large scale without requiring pre-existing information. In this study, we employed HTS to investigate viral pathogens infecting citrus in three different regions of South Korea: Jejudo (Jeju), Wando-gun (Wando), and Dangjin-si (Dangjin). The results unveiled diverse viruses and viroids that exhibited regional variations. Notably, alongside the identification of well-known citrus viruses such as satsuma dwarf virus, citrus tatter leaf virus, and citrus leaf blotch virus (CLBV), this study also uncovered several viruses and viroids previously unreported in Korean citrus. Phylogenetic analysis revealed that majority of identified viruses exhibited the closest affilations with isolates from China or Japan. However, CLBV and citrus viroid-I-LSS displayed diverse phylogenetic positions, reflecting their regional origins. This study advances our understanding of citrus virome diversity and regional dynamics through HTS, emphasizing its potential in unraveling intricate viral pathogens in agriculture. Consequently, it significantly contributes to disease management strategies, ensuring the resilience of the citrus industry.

참당귀 뿌리 직경별 지표성분 함량 및 항산화 활성 분석 (Analysis of Index Component Content and Antioxidant Activity According to the Root Diameter of Angelica gigas Nakai)

  • 이상훈;이소희;김미란;홍충의;허목;한종원;이우문;윤형묵;김연복;이이;구성철
    • 한국자원식물학회지
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    • 제32권2호
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    • pp.116-123
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    • 2019
  • 본 연구는 참당귀 뿌리의 가공 과정을 통해 분류되는 신, 대미, 중미, 세미 부위에서 지표성분과 항산화 활성을 분석함으로써, 약재로 이용되지 않는 세미 부위의 부산물 활용가능성을 평가하기 위해 수행하였다. 참당귀 뿌리의 가공 시 세미 부위의 회수율은 100kg당 $15{\pm}1.0%$로 비교적 많은 부분을 차지하였다. 지표성분 함량은 참당귀 뿌리의 직경이 작을수록 증가하는 경향성을 나타내었으며, 세미 부위에서 decursin ($4.42{\pm}0.27%$)과 decursinol angelate ($4.46{\pm}0.28%$)의 함량이 가장 높았다. 항산화 활성도 뿌리의 직경이 작아질수록 증가하는 경향성을 나타내었으며, 세미 부위의 항산화 활은 유통되는 한약재와 유사하거나 우수하였다. 세미의 지표성분 함량 및 항산화 활성의 우수성과 회수율을 고려하면, 참당귀 부산물인 세미를 건강기능성 식품, 화장품 등의 추출물 원료로 활용이 가능할 것으로 생각된다.

한약재 세척 방법에 관한 연구 (The Review on the Washing System of Herb medicine)

  • 현자경;서영배
    • 혜화의학회지
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    • 제20권1호
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    • pp.161-174
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    • 2011
  • Objectives : This paper researched methods of washing Herb medicine through research papers of washing Crops Materials and Methods : We collected research papers on Washing crops. Then we analysed them according to washing methods. Results : The following results were obtained in this study. 1. Herbal fruit, wash using a conveyor belt. 2. Leafy herbs, soak in a chlorine solution or ozone solution and rinse it. sometimes using micro bubble system. 3. Radix, wash with high pressure sprinkling water. 4. Cortex, wash under running water by hand washing. Conclusion : Herb medicines need a washing methods for each region. so Herbs should be washed in a suitable methods for each region.

SRAP을 이용한 국내육성 심비디움 품종의 유전적 다양성 분석 (Analysis of Genetic Diversity in Cymbidium Varieties Using SRAP)

  • 박부희;김미선;이영란;박필만;이동수;예병우
    • 한국육종학회지
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    • 제43권5호
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    • pp.399-404
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    • 2011
  • Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between $F_1$ varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.

동반작물 주위재배에 의한 배추 벼룩잎벌레 억제효과 (Suppressive Effect of Repellent Plant Cultivation against Striped Flea Beetle of Chinese Cabbage)

  • 한은정;최재필;김용기;홍성준;박종호;심창기;김민정;김석철
    • 한국유기농업학회지
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    • 제23권4호
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    • pp.911-921
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    • 2015
  • 본 연구는 배추에서 벼룩잎벌레의 생태적 관리기술을 개발하고자 실험실과 포장 야외포장에서 수행되었다. 허브 식물인 바질과 레몬밤의 벼룩잎벌레에 대한 기피활성을 실험실내에서 후각계를 활용하여 측정한 결과, 벼룩잎벌레가 바질과 레몬밤을 기피하는 결과를 얻을 수 있었다. 선발된 기피식물과 유인식물로 알려진 겨자류 작물을 대상으로 포장내에서 간작과 주위작 처리했을 때의 벼룩잎벌레 억제효과를 평가하였다. 식물자원의 간작 재배효과는 시험결과 실내실험과는 달리 레몬밤 간작처리구에서 벼룩잎벌레의 밀도가 높았으며, 바질 간작처리구와 돌산갓 간작처리구에서 벼룩잎벌레의 밀도가 낮았다. 식물자원의 주위작 재배효과 시험결과 적겨자 주위작 처리와 배추 단독처리구에서 벼룩잎벌레의 밀도가 가장 높았으며, 바질과 레몬밤에서는 벼룩잎벌레가 거의 발생하지 않았다. 벼룩잎벌레 기피식물인 바질과 레몬밤 주위재배를 통해 유기농 배추 재배지에서 벼룩잎벌레의 발생을 예방할 수 있을 것으로 판단된다.

중점관리기준에 기초한 국내생산 당귀의 산지 수확 후 아플라톡신의 안전성 평가 연구 (Safety Evaluation from Aflatoxin risk of Korean Angelicae Gigantis Radix Based on Critical Control Points)

  • 최혜진;안태진;안영섭;박충범;김주일;박성환;양현;도기헌;문유석
    • 한국약용작물학회지
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    • 제19권1호
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    • pp.47-53
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    • 2011
  • HACCP methodology was applied in the post-harvest processing and storage of domestic medicinal produces. Particularly in terms of mold and mycotoxin contamination, candidate critical control points (CCP) in the conventional practice in Korean farms were selected and monitored by comparing with on the standard guided processing and storage. When each processing of Angelicae Gigantis Radix were assessed for their safety, the drying steps such as the sun drying or the thermal drying depending on each farm made differences in mold contamination. Moreover, the storage conditions before or after the processing were another critical determinant in the fungal contamination. In other words, storage under $4{\circ}C$ rather than at room temperature was favorable for reducing mold growth in the harvested crops. Occurrence rate of Aflatoxin $B_1 \;(AFB_1)$ in Angelicae Gigantis Radix were 12.8%, but amount of $AFB_1$ in all the collected samples were below 10 ppb regulatory limit allowed in Korea. However, for a few samples of Angelicae Gigantis Radix, still relatively high levels of total amount of the major aflatoxins (aflatoxin $B_1\; +\; B_2\; +\; G_1\; +\; G_2$) were observed around 0.18~49.94 ppb, which is not regulated presently in Korea. It thus can be suggested that post-harvest processing and storage of Korean medicinal crops need further investigation and monitoring to establish the Good Agricultural Practice (GAP), particularly to minimize microbial risk including mold and mycotoxin contamination under the changing climate. Additionally, it is also warranted for new enacting of regulatory limits for total aflatoxins in the medicinal crops.

유기재배 사과과원의 피복작물별 잡초발생 (Weed Occurrence in Organic Apple Orchards by Different Cover Crops)

  • 오영주;송양익;강석범;홍선희;최진호;백원기
    • Weed & Turfgrass Science
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    • 제1권4호
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    • pp.24-30
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    • 2012
  • 유기 사과과원의 피복작물 선정을 위해 피복작물의 피복율과 시기별 잡초의 발생양상에 대해 조사하였다. 토양의 화학성분은 자연초생구에 비해 들묵새와 화이트클로버가 유기물 함량과 인산 함량이 다소 높게 분석되었다. 피복작물별 잡초 종의 출현은 5월에는 클로버 종류와 청보리, 호밀이 비교적 잡초 종의 출현이 억제되었고 6월에는 호밀이 가장 좋은 효과를 가져왔다. 7월에는 피복율이 낮은 톨페스큐와 켄터키블루그라스에서는 많은 잡초 종이 출현하였다. 유기 사과과원의 발생한 잡초는 총 51종으로 조사되었고 피복율을 높이기 위해서는 피복식물 보다 키가 크고 광엽인 좀명아주, 명아주, 환삼덩굴 등의 봄철 제거가 필요하다. 잡초의 건물중을 측정한 결과 피복율이 가장 낮았던 켄터키블루그라스에서 대조구와 거의 유사한 결과를 보여주었다. 잡초와 토양과의 관계에 대해 CCA로 분석한 결과 1축에서는 토끼풀과 여뀌가 유기물의 함량과 높은 상과관계를 가지며 명아주는 인산의 함량과 상관관계를 가지고 있는 것으로 분석되었다.