Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Analysis of Genetic Diversity in Cymbidium Varieties Using SRAP

SRAP을 이용한 국내육성 심비디움 품종의 유전적 다양성 분석

  • Park, Pue Hee (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Mi Seon (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Young Ran (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Pil Man (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Dong Soo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yae, Byeong Woo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 박부희 (국립원예특작과학원 화훼과) ;
  • 김미선 (국립원예특작과학원 화훼과) ;
  • 이영란 (국립원예특작과학원 화훼과) ;
  • 박필만 (국립원예특작과학원 화훼과) ;
  • 이동수 (국립원예특작과학원 화훼과) ;
  • 예병우 (국립원예특작과학원 화훼과)
  • Received : 2011.08.02
  • Accepted : 2011.10.14
  • Published : 2011.12.30
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Abstract

Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between $F_1$ varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.

Keywords

References

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