• 대한한방소아과학회지
• /
• 제34권4호
• /
• pp.43-58
• /
• 2020

Objectives The purpose of this study is to analyze the effect of various herbal medicin on gut microbiota. Methods Electronic searches were performed using NDSL, OASIS, KISS, KMBASE, K-portal, Pub med, Cochrane, CNKI. Results we analyzed 25 experimental studies on the effect of herbal medicine on microbiota. Diabetes, obesity, inflammatory bowel disease have been frequently studied in micobiota-related disease. The most common experimental animal model used in the studies C57BL/7 mouse. Among the studies wherein single herbal medication were used, Gynostemma pentaphyllum was most commonly studies, and different herbal medications were used in the studies wherein complex herbal medications were studied. Next generation sequencing was performed using Illumina MiSeq system, and gut microbiota analysis was performed using QIIME and Ribosomal Database Project (RDP). In most studies, the herbal medicines exerted regulatory effects on gut microbiota and improved the symptoms of the experimental groups. Conclusions This review provides basic data on the correlation between korean medicine and gut microbiota, as well as information for the development of korean medicine.

• BMB Reports
• /
• 제55권9호
• /
• pp.417-428
• /
• 2022

Herbal medicine, a multi-component treatment, has been extensively practiced for treating various symptoms and diseases. However, its molecular mechanism of action on the human body is unknown, which impedes the development and application of herbal medicine. To address this, recent studies are increasingly adopting systems pharmacology, which interprets pharmacological effects of drugs from consequences of the interaction networks that drugs might have. Most conventional network-based approaches collect associations of herb-compound, compound-target, and target-disease from individual databases, respectively, and construct an integrated network of herb-compound-target-disease to study the complex mechanisms underlying herbal treatment. More recently, rapid advances in high-throughput omics technology have led numerous studies to exploring gene expression profiles induced by herbal treatments to elicit information on direct associations between herbs and genes at the genome-wide scale. In this review, we summarize key databases and computational methods utilized in systems pharmacology for studying herbal medicine. We also highlight recent studies that identify modes of action or novel indications of herbal medicine by harnessing drug-induced transcriptome data.

• 한방안이비인후피부과학회지
• /
• 제29권1호
• /
• pp.131-144
• /
• 2016

Objective : This study is to review the effect of herbal medicines on treatment of Allergic Rhinitis in animal models reported in Korean domestic journals.Methods : The Databases(NDSL, Koreantk, OASIS, KISS) are searched with terms as Allergic Rhinitis. among them, only animal study reports were reviewed. Animal model, intervention, and observation methods of measuring indicators were extracted.Results : 27 articles were reviewed. All studies used OVA to induce Allergic Rhinitis. 7 studies used simple herbal medicines, 20 studies used complex herbal medicines, To evaluate the effect of the Herbal medicines, 23 studies used immunological examination, 18 studies used histological examination, 16 studies used hematologic examination, 3 studies used macroscopy The evidences of 5 studies is insufficient to show that Herbal medicines are effective for treatment of Allergic Rhinitis.

• 대한한방부인과학회지
• /
• 제36권1호
• /
• pp.35-53
• /
• 2023

Objectives: The aim of this study is to investigate the research trends of animal experiment on the herbal medicine treatment for endometriosis over the past 10 years. Methods: We searched the databases (Pubmed, Embase, Science-on) with terms as endometriosis, Korean medicine, herbal medicine and Chinese medicine. Among the results, we reviewed studies published from 2013 to 2022. Results: 26 studies were selected and analyzed. 8 single herbs and 18 complex formula were used in these studies. The most commonly used herbal medicine for endometriosis was Paeoniae Radix. Each studies showed that herbal medicine had significant effect on treating endometriosis. Conclusions: These results suggest that treating endometriosis with herbal material is effective in inhibiting ectopic lesion and angiogenesis, increasing pain threshold and apoptosis. Further research should be conducted to verify its therapeutic mechanisms and effectiveness.

• 대한본초학회지
• /
• 제29권6호
• /
• pp.157-163
• /
• 2014

Objectives : Gamisoyo-san complex prescription were made with Angelicae Gigantis Radix, Paeoniae Radix, Atractylodes rhizome white, Hoelen, Bupleuri Radix, Moutan Cortex Radicis, Gardeniae Fructus, Zingiberis Rhizoma Crudus, Menthae Herba. The purpose of this study was to research the whitening effect of the extract from Gamisoyo-san, which is one of the used herbal complex prescription. Methods : This study investigated inhibitory effect of Gamisoyo-san in tyrosinase activity. Cell viability were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Then, Gamisoyo-san measured reversed-transcription-PCR for mRNA expression using B16F10 mouse melanoma cells. Results : For whitening effects, the tyrosinase inhibition effect of extract was shown to 52.4% at $5,000{\mu}g/m{\ell}$ concentration. The cell viability on B16F10 melanoma cells of Gamisoyo-san extract showed higher than 75% at $1,000{\mu}g/m{\ell}$ concentration. In this study, an experiment was performed by setting the non-toxic concentration range of 50, 150, $250{\mu}g/m{\ell}$. The Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a positive control. The microphthalmia-associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), tyrosinase mRNA expression inhibitory by reverse transcription-PCR of Gamisoyo-san extract were decreased by 95.3%, 98.8%, 96.3% and 49.5% at $250{\mu}g/m{\ell}$ which the highest concentration. Conclusions : All these findings could verify that whitening effects of Gamisoyo-san extract by tyrosinase inhibitory activity and mRNA expression. The Gamisoyo-san could be used as material for functional cosmetics, such as skin whitening products.

• 대한약침학회지
• /
• 제21권3호
• /
• pp.203-206
• /
• 2018

Objectives: There is an increasing number of complex diseases that are progressively more difficult to be controlled using the conventional "single compound, single target" approach as demonstrated in our current modern drug development. TCM might be the new cornerstone of treatment alternative when the current treatment option is no longer as effective or that we have exhausted it as an option. Orthogonal stimulus-response compatibility group study is one of the most frequently employed formulas to produce optimal herbal combination for treatment of multi-syndromic diseases. This approach could solve the relatively low efficacy single drug therapy usage and chronic adverse effects caused by long terms administration of drugs that has been reported in the field of pharmacology and medicine Methods: The present review was based on the Science Direct database search for those related to the TCM and the development of antihypertensive TCM herbal combination using orthogonal stimulus-response compatibility group studies approach. Results: Recent studies have demonstrated that the orthogonal stimulus-response compatibility group study approach was most frequently used to formulate TCM herbal combination based on the TCM principles upon the selection of herbs, and the resulting formulated TCM formula exhibited desired outcomes in treating one of global concerned complex multi-syndromic diseases, the hypertension. These promising therapeutic effects were claimed to have been attributed by the holistic signaling mechanism pathways employed by the crude combination of herbs. Conclusion: The present review could serve as a guide and prove the feasibility of TCM principles to be used for future pharmacological drug research development.

• 인간식물환경학회지
• /
• 제24권6호
• /
• pp.663-672
• /
• 2021

Background and objective: As the 'Healing Agriculture Research and Development and Promotion Act' came into effect on March 25, 2021, social interest in agro-healing has been increasing exponentially. This study was conducted to analyze the priorities of agro-healing classification system and to provide basic data to inform policy directions and related research for the development and activation of agro-healing. Methods: The survey data collected from 18 experts were analyzed using the analytic hierarchy process (AHP) method in determine the relative weights of the main and sub-criteria for the classification system. There were three main criteria identified: agro-healing input industry, agro-healing service industry, and agro-healing-related/derived industry. There were also 11 sub-criteria. Results: The top three sub-criteria with the highest complex weights include "community service," "social rehabilitation" and "treatment and rehabilitation," all of which correspond to the main criterion "agro-healing service industry". In addition, the complex weights of the sub-criteria corresponding to the main criterion "agro-healing related/derived industry" are as follows: "other agro-healing support service," which ranked 4th, and "training and education institutions for agro-healing experts," which ranked 5th when prioritizing the criteria. Conclusion: The results of this study suggest that the main criterion to be considered first in establishing a classification system is "agro-healing service industry". Therefore, it is necessary to continue research on detailed service classifications and systems with verified validity to ensure expertise in human resources, and organization related to social purpose services in agro-healing.

• 한국자원식물학회지
• /
• 제25권3호
• /
• pp.339-345
• /
• 2012

The marine carotenoid fucoxanthin can be found in marine brown seaweeds, macroalgae, diatoms, and microalgae, and has remarkable biological properties. Numerous studies have shown that fucoxanthin has considerable potential and promising applications in human health, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We here investigated the mechanisms by anti-allergic activity of fucoxanthin fraction from Eisenia bicyclis in immunoglobulin E-antigen complex (IgE/DNP-BSA)-stimulated RBL-2H3 mast cells. This study we found that the fucoxanthin inhibits the release of ${\beta}$-hexosaminidase and suppressed not only transcriptional activation of NF-${\kappa}B$, but also phosphorylation of ERK and JNK in IgE/DNP-BSA-treated RBL-2H3 cells. Fucoxanthin may be useful for preventing allergic diseases, including asthma and atopic dermatitis.

• 대한한의학원전학회지
• /
• 제36권2호
• /
• pp.23-34
• /
• 2023

Objectives : We propose a method to disassemble Traditional East Asian Medicine herbal formulas using machine learning. Methods : After creating a model using Byte Pair Encoding(BPE) and G-Score, the model was trained with training data. Afterwards, the learned model was applied to the test data, of which the results were compared with expert opinion. Results : The results acquired through the model were not significantly different from those of modern expert opinions. However, there were cases where the meaning was partially unclear, while there were cases where new knowledge could be obtained through the disassembling process. Conclusions : It is expected that disassembling herbal formulas through the proposed method in this study will help save resources required to understand complex ones.

• 대한예방한의학회지
• /
• 제28권1호
• /
• pp.59-67
• /
• 2024

Objectives : This study aims to systematically examine the determinants of the intention to take herbal medicine among individuals with experience in Korean medicine. Methods : This study utilized the 3,245 respondents from the 2020 Korean Medicine Utilization and Herbal Medicine Consumption Survey who reported having used Korean medical services; the responses were selected through a complex sample analysis, and analytics techniques including frequency analysis, Rao-scott chisquare test, and logistic regression were used to analyze the responses. Results : The results of data analysis reveal that the intention to take herbal medicine in the future among these individuals was significantly influenced by factors such as enrollment in private health insurance, the facility environment of Korean medical institutions, and treatment outcomes. Conclusions : Therefore, it is imperative for Korean medical institutions to continuously try reasonable action strategies, including improving medical facilities/environments and building institutional mechanisms among medical staff and members to enhance the quality of Korean medical services. Additionally, the Ministry of Health and Welfare needs to make policy efforts to reduce patients' medical expenses, such as reducing the co-insurance rate in the pilot project of applying health insurance to herbal medicine.