• The Korea Journal of Herbology
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• v.32 no.4
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• pp.25-32
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• 2017

Objectives : Chungsimyeonja-eum (CSYJE; Qingxinlianzi-yin in Chinese; Seishinrenshi-in in Japanese), a traditional herbal medicine, has been used for treating mouth dryness, hyperuresis. This study was designed to determine preservation period of CSYJE. We investigated the stability and biological activity of CSYJE depending on the preservation temperature and periods. Methods : CSYJE decoction was preserved for 0-6 or 12 months at room temperature (RT, $23{\pm}1^{\circ}C$) or refrigeration ($4^{\circ}C$). To evaluate the stability of CSYJE decoction, pH and dissolved solids content were measured. High-performance liquid chromatography (HPLC) analysis was performed to determine marker compounds-liquiritin apioside, liquiritin, baicalin, wogonoside, and glycyrrhizin-in CSYJE. To determine anti-inflammatory effect of CSYJE, prostaglandin $E_2$ ($PGE_2$) was measured in LPS-stimulated RAW 264.7 macrophages. Results : Both at RT and $4^{\circ}C$, pH was decreased depending on the preservation periods. There was no changes in dissolved solids content depending on the preservation temperature and periods. Both at RT and $4^{\circ}C$, the contents of liquiritin apioside and liquiritin were slightly increased at 1 month of storage. The level of baicalin was decreased time-dependently and the disappearance rate at RT is larger than at $4^{\circ}C$. CSYJE inhibited $PGE_2$ production in LPS-stimulated RAW 264.7 cells and maintained inhibitory effect by 12 months both at RT and $4^{\circ}C$. Conclusions : Based on the disappearance rate of the baicalin in CSYJE, the preservation period is recommended within 8 months for RT and 12 months for $4^{\circ}C$.

• The Journal of Korean Medicine
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• v.44 no.4
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• pp.193-207
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• 2023

Objectives: The purpose of this study was to evaluate the effectiveness and safety of traditional herbal medicine as a stand-alone treatment group through major English databases due to the lack of RCTs in Korea, and to provide a review of the herbal interventions used. Methods: Using four databases (Pubmed, EMBASE, OASIS, RISS), combination of words such as "Coronavirus" "RCT" "Herb" "Decoction" "TCM" were used. RCTs using herbal medicines to treat coronavirus were searched. Final 4 studies were selected by two authors according to inclusion and exclusion criteria. Results: A total of 1,435 patients were studied. The Chinese herbs used in the treatment group were Shengmai Yin, JingYinGuBiao granules, Jinhua Qinggan granules, and Bufei Huoxue capsules. The intervention group showed greater attenuation of pneumonia lesions on CT. Also, improvement in 6-min walk distance (6MWD), and negative conversion rate in treatment group were reported. Furthermore, scores on the Fatigue Assessment Inventory (FAI) were lower in the herbal group than in the placebo group. The median time to recovery of COVID-19 related symptoms was shorter in TCM group compared to the control group. Reported adverse effects were diarrhea, liver dysfunction, and excessive menstruation, and two papers did not mention side effects in detail. Conclusion: Herbal medicine alone can increase the conversion rate of viral negativity and relieve COVID-19 related symptoms without significant adverse effects.

• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.1
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• pp.43-56
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• 2018

Objectives: This study was designed to investigate the basis for developing the herbal decoction that may help promote healthy blood vessels in accordance with the basic principles of Korean medicinal materials. Methods: In this study, we investigated the vascular relaxation effects of fifty herbal decoctions on vascular response in isolated thoracic aorta of phenylephrine-preconracted rats. Results: The results of identify the vascular relaxation effect of fifty herbal decoctions provided by Korea Institute of Korean Medicine, twenty-one herbal decoctions showed vascular relaxation effects. Among them, four herbal decoctions, Mahwang-tang (麻黃湯), Samchulgeonbi-tang (參朮健脾湯), Cheonwangbosim-dan (天王補心丹), and Socheonglyong-tang (小靑龍湯), with showed significantly concentration-dependent vasodilation was examined. Vascular relaxation level are $84.02{\pm}4.70$, $79.39{\pm}13.9$, $51.26{\pm}12.56$, and $54.73{\pm}15.8%$, respectively (P<0.05, 0.01, and 0.001). Conclusions: Thus these results provide a basic data for the treatment of the impairment of vascular function and blood pressure in traditional Oriental medicine.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.5
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• pp.967-975
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• 2002

This study was to investigate the metal accumulation from Ohjeoksan-Decoction to rat brain(Sprague Dawley). 1. In Control group, each metal concentration was within 26.65mg/kg, and in each experimental groups, was within 28.39mg/kg. And, there were no significant metal concentration between control and experimental groups. Exceptionally, Cr and Ni level of control group was lower than experimental groups significantly. (p<0.05) and Cr and Ni level of experimental I group was lower than other experimental groups too.(p<0.05). But, Pb level of control group was higher than experimental groups significantly.(p<0.05) Pb level of experimental I group was higher than other experimental groups(p<0.01). 2. In non-hazardous, hazardous and total metal concentration, there was no increase tendency in brain according to the high dose of OD(Ohjeoksan-Decoction) intake. Reversely, in experimental groups, hazardous metal concentration was decrease by high OD intake.(p<0.05). 3. Correlation among each metal in brain was various in each groups. Briefly under the intake of Ohjeoksan-Decoction, that is very busy herb prescription, this study was defined within safety in brain metal level by P.O. during 10 days. But, there should be a more research about Pb increase by high dose OD intake

• 대한상한금궤의학회지
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• v.4 no.1
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• pp.13-28
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• 2012

Objective : The purpose of this study is to suggest medical approach to musculoskeletal system disorders using the decoction of Sanghan-Geumgwe. We studied cases of Ling-Gui-Gan-Zao-Tang prescribed patients to evaluate the clinical efficacy in musculoskeletal system disorders Method : We devised medical approach of Sanghan-Geumgwe in musculoskeletal disorders as follows. First, we chose ryeon-je(攣劑) and soo-je(水劑) herb medicine, commonly used in musculoskeletal disorders. In the selected herb group, we designated ryeon-je(攣劑) to be first key herbs, Soo-Je(水劑) as the second key herb, and other herb groups as third key herb. In this sequential selection and exclusion process, herbs were chosen based upon yak-neung-hyo-seon (藥能效選). Combination of those selected herbs drew pre-prescription group, finally prescription were made by the prescription criteria. Results : Based on the medical approach of decoctions of Sanghan-Geumgwe, we chose Ling-Gui-Gan-Zao-Tang to treat many kinds of musculoskeletal system disorders. And we achieved higher results on treatment for musculoskeletal system disorders. Conclusions : The medical approch using the decoctions of Sanghan-Geumgwe is very useful in choosing accurate prescriptions for patients with musculoskeletal system disorders in clinic.

• Korean Journal of Food Science and Technology
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• v.40 no.6
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• pp.649-655
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• 2008

In this study, the characteristics of the lactic fermentation of gamju manufactured using a medicinal herb decoction were assessed. A bacterial strain, LAB19, which is used for the induction of lactic fermentation into gamju, was isolated from dried persimmon and identified as Leuconostoc mesenteroides on the basis of morphological, physiological, and chemotaxonomical features, and 16S rRNA sequencing analysis. After 60 hours of lactic fermentation with Leuconostoc mesenteroides LAB19 at $25^{\circ}C$, the gamju was determined to contain 141.3 g/L of reducing sugar, 5.33 g/L of acids, and 1.19 g/L of soluble phenolics. Approximately 90% of reducing sugar and 58% of acids were maltose and lactic acid, respectively. Free radical scavenging activities were retained at levels between 76.6 to 75.7% during the lactic fermentation of gamju.

• Journal of Society of Preventive Korean Medicine
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• v.4 no.2
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• pp.242-257
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• 2000

This study was to investigate the metal accumulation from Ohjeoksan-Decoction to rat brain(Sprague Dawley). 1. In Control group, each metal concentration was within 26.65mg/kg. and in each experimental groups, was within 28.39mg/kg. And there were no significant metal concentration between control and experimental groups. Exceptionally, Cr and Ni level of control group was lower than experimental groups significantly. (p<0.05) and Cr and Ni level of experimental I group was lower than other experimental groups too. (p<0.05). But, Pb level of control group was higher than experimental groups significantly (p<0.05) Pb level of experimental I group was higher than other experimental groups(p<0.01) 2. In non-hazardous, hazardous and total metal concentration, there was no increase tendency in brain according to the high dose of OD(Ohjeoksan-Decoction) intake. Reversely, in experimental groups, hazardous metal concentration was decrease by high OD intake. (P<0.05) 3. Correlation among each metal in brain was various in each groups Briefly under the intake of Ohjeoksan-Decoction, that if very busy herb prescription , this study was defined within safety in brain metal level by P.O. during 10 days. But, there should be a more research about Pb increase by high dose OD intake

• The Journal of Korean Obstetrics and Gynecology
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• v.28 no.2
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• pp.76-90
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• 2015

Objectives : This study were invesitigated papers published in international journals to assess the effect of Korean Medicine treatments for menopausal depression. Through this review, we aimed to research more effective ways of menopausal depression treatment. Methods : The database of this review was created through searching PubMed from Jan, 2005 to Feb, 2015 : using terms of ‘menopausal depression’ and ‘post menopausal depression’ with ‘acupuncture’, ‘alternative medicine’, ‘CAM’ and ‘herbal medicine’. Results : 119 studies were analyzed but some of them were excluded due to lack of relevance or noncomformity of subjects. Finally, 16 studies were included in this review. Conclusions : In 16 studies, the acupuncture and herb decoction had significant interventions to improve menopausal depression. Therefore Acupuncture and herbal medicine may be effective treatment method of menopausal depression.

• Korean Journal of Pharmacognosy
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• v.47 no.1
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• pp.62-72
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• 2016

Banhasasim-tang is a well-known traditional Korean herbal formula and has been used clinically for the treatment of gastric disease, including acute and chronic gastritis, diarrhea and gastric ulcers in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer method was developed for the quantitative determination of the 13 marker constituents, homogentisic acid (1), 3,4-dihydroxybenzaldehyde (2), spinosin (3), liquiritin (4), baicalin (5), ginsenoside Rg1 (6), liquiritigenin (7), wogonoside (8), ginsenoside Rb1 (9), baicalein (10), glycyrrhizin (11), wogonin (12), and 6-gingerol (13) in Banhasasim-tang decoction. Separation of the compounds 1-13 was using an UPLC BEH $C_{18}$ ($100{\times}2.1mm$, $1.7{\mu}m$) column and column oven temperature was maintained at $45^{\circ}C$. The mobile phase consisted of 0.1% (v/v) formic acid in water (A) and acetonitrile (B) by gradient elution. The injection volume and flow rate were $2.0{\mu}L$ and 0.3 mL/min, respectively. Calibration curves of the compounds 1-13 were showed with $r^2$ values ${\geq}0.9908$. The limit of detection and limit of quantification values of the compounds 1-13 were 0.04-1.11 ng/mL and 0.13-3.33 ng/mL, respectively. Among the these compounds, the compounds 1-3 were not detected, while the compounds 4-13 were detected in the ranges of $3.20-107,062.98{\mu}g/g$ in Banhasasim-tang sample.

• YAKHAK HOEJI
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• v.60 no.2
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• pp.64-72
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• 2016

Jakyakgamcho-tang is a well-known traditional herbal medicine and has been used for the treatment of mainly pains in oriental medicine. In this study, analytical method for the quantitative determination of the eleven marker components, gallic acid (1), oxypaeoniflorin (2), paeoniflorin (3), albiflorin (4), liquiritin (5), isoliquiritin (6), ononin (7), liquiritigenin (8), benzoylpaeoniflorin (9), paeonol (10), and glycyrrhizin (11) in Jakyakgamcho-tang decoction was performed using an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer. The analytical column for separation of the compounds 1~11 was used an UPLC BEH $C_{18}$ ($100{\times}2.1mm$, $1.7{\mu}m$) column and column oven temperature was maintained at $45^{\circ}C$. The mobile phase consisted of 0.1% (v/v) aqueous formic acid (A) and acetonitrile (B) by gradient elution. The flow rate was 0.3 ml/min and injection volume was $2.0{\mu}l$. Correlation coefficient in the calibration curves of the compounds 1~11 were showed a good linearity with more than 0.99. The limit of detection and limit of quantification values of the compounds 1~13 were detected in the ranges 0.06~18.43 ng/ml and 0.18~58.29 ng/ml, respectively. Among the compounds 1~11, the compounds 10 were not detected in this sample, while the ten compounds, 1~9 and 11, were detected $44.05{\sim}19,289.05{\mu}g/g$ in Jakyakgamcho-tang extract.