The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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A Study on Change of Marker Compounds and Biological Activity in Chungsimyeonja-eum Decoction Depending on A Storage Temperature and Periods

보관 온도 및 기간에 따른 청심연자음 전탕액의 지표성분과 약리 활성 변화에 관한 연구

  • Yoo, Sae-Rom (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hyekyung (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Nari (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (K-herb Research Center, Korea Institute of Oriental Medicine)
  • 유새롬 (한국한의학연구원 K-herb연구단) ;
  • 하혜경 (한국한의학연구원 K-herb연구단) ;
  • 이나리 (한국한의학연구원 K-herb연구단) ;
  • 신현규 (한국한의학연구원 K-herb연구단) ;
  • 서창섭 (한국한의학연구원 K-herb연구단)
  • Received : 2017.04.10
  • Accepted : 2017.07.15
  • Published : 2017.07.30
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Abstract

Objectives : Chungsimyeonja-eum (CSYJE; Qingxinlianzi-yin in Chinese; Seishinrenshi-in in Japanese), a traditional herbal medicine, has been used for treating mouth dryness, hyperuresis. This study was designed to determine preservation period of CSYJE. We investigated the stability and biological activity of CSYJE depending on the preservation temperature and periods. Methods : CSYJE decoction was preserved for 0-6 or 12 months at room temperature (RT, $23{\pm}1^{\circ}C$) or refrigeration ($4^{\circ}C$). To evaluate the stability of CSYJE decoction, pH and dissolved solids content were measured. High-performance liquid chromatography (HPLC) analysis was performed to determine marker compounds-liquiritin apioside, liquiritin, baicalin, wogonoside, and glycyrrhizin-in CSYJE. To determine anti-inflammatory effect of CSYJE, prostaglandin $E_2$ ($PGE_2$) was measured in LPS-stimulated RAW 264.7 macrophages. Results : Both at RT and $4^{\circ}C$, pH was decreased depending on the preservation periods. There was no changes in dissolved solids content depending on the preservation temperature and periods. Both at RT and $4^{\circ}C$, the contents of liquiritin apioside and liquiritin were slightly increased at 1 month of storage. The level of baicalin was decreased time-dependently and the disappearance rate at RT is larger than at $4^{\circ}C$. CSYJE inhibited $PGE_2$ production in LPS-stimulated RAW 264.7 cells and maintained inhibitory effect by 12 months both at RT and $4^{\circ}C$. Conclusions : Based on the disappearance rate of the baicalin in CSYJE, the preservation period is recommended within 8 months for RT and 12 months for $4^{\circ}C$.

Keywords

References

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