• YAKHAK HOEJI
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• v.46 no.5
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• pp.352-357
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• 2002

The three crude drugs of the Kalopanax pictus (Araliaceae) roots (K), Pueraria thunbergiana (Leguminosae) flowers (P), and the Rhus verniciflua (Anacardiaceae) heartwood(R) used for anti-thirst drugs in Oriental herb medicine were extracted with MeOH, respectively, and the successive fractionation of the extract gave EtOAc extract. Certain amount ratios of the three extracts were also prepared to compare the antimutagenicity in Ames test. In N-methyl-N(-nitro-N-nitrosoguanidine (MNNG; 0.4 (g/plate)-induced test, the activities of complex mixture were observed between the highest antimutagenic activity of K extract and the lowest P extract. In aflatoxin (AFB$_1$)-induced test, the EtOAc complex (K : P : R=l : 1 : 3) labeled E-113 decreased the revertants of Salmonella typhimurium TA100 by 95％, which activity were highest among other extracts or complexes mixture used. Fractionation of organic solvent mostly increased the antimutagenicity. These trends were also observed in the antimutagenicity test of the mixture of each active component of kalopanaxsaponin A, tectorigenin and sulfuretin. These results supported that many kinds of anti-thirst herb medicine in the prescription could effectively prevent cancer disease.

• Journal of Life Science
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• v.32 no.7
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• pp.523-531
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• 2022

The purpose of this study was to evaluate the effect of antioxidant and anti-adipogenic activities in ethanol extracts from herb mixture (Ephedra sinica, Atractylodes lance, Gypsum fibrosum, and Theobroma cacao). DPPH, ABTS⁺ radical and xanthine oxidase scavenging activities were measured for antioxidant activity. Extracts of the herb mixture had 75.0, 100.8, and 79.5% scavenging activities at 1,000 ㎍/ml concentration, respectively. We investigated the inhibition of adipogenesis and adipocyte differentiation with an extract of an herb mixture in 3T3-L1 preadipocytes. An extract from the herb mixture at concentrations between 0 and 50 ㎍/ml did not affect 3T3-L1 cell viability. Treatment with herb mixture extracts of 25, 50, and 75 ㎍/ml in 3T3-L1 preadipocytes inhibited lipid accumulation in a dose-dependent manner. As a result of a Western blot experiment, it was shown that the herb mixture inhibited the differentiation transcription factors, PPARγ and C/EBPa, by 44.2 and 77.6%, respectively, at a concentration of 75 ㎍/ml in MDI-induced differentiated 3T3-L1 cells. As a result of RT-PCR, the gene expression of C/EBPa, SREBP-1c, and PPARγ was significantly inhibited by 43.4%, 59.6%, and 55.3%, respectively, at the concentration of 75 ㎍/ml of the herb mixture compared with the MDI-treated group. In addition, the expression of fatty acid synthase (FAS), a fatty acid synthesis regulator, was suppressed. These results can be applied to develop a functional food for anti-obesity with a herb mixture.

• Fashion & Textile Research Journal
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• v.21 no.1
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• pp.112-119
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• 2019

This study had a purpose of further investigating and developing the usage of the eco-friendly functional dye that has physiological and pharmacological functions. This study was implemented complex dyeing method which used both natural dyes such as Gardenia jasminoides J.Ellis colorant, which are the yellow dyes that have high brightness and low chroma, and Xanthium strumarium L. extract. The silk fabrics were used to dye in various methods. They were also used to investigate functionalities dyed fabrics by various treating method, such as their color fastness, antibacterial activities and deodorization, the following were obtained. The fabrics that were dyed with herbs had decreasing brightness as the herbs extract concentrations were increased and had subtly changing chroma. But, because their K/S values were under 0.5, they were not properly dyed. Color fastness differed according to the natural dyes and herbs' complex dyeing order. The Color fastness was good when the fabrics were dyed with the colorant first, and then with the herb. When the fabrics were dyed with mixture dyeing solution, the natural dye and the herb extract, the herb didn't affect the dyeabilities of fabrics as much. When the fabrics were dyed with the mixture of the colorant and the herb extract, the dyeabilities didn't have much changes. However, their color fastnesses were improved a little bit due to the complex dyeing condition. When the fabrics were dyed with the mixture of the colorant and the herbs extract, their antibacterial activities and deodorization were increased.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.57 no.4
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• pp.355-363
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• 2024

Although aquaculture shrimp production has increased, disease mortality has also increased. Acute hepatopancreatic necrosis disease (AHPND) is an emerging disease that significantly reduces production. In this study, we have investigated the potential of medicinal herbs in enhancing the immune system of shrimp. A mixture of medicinal herb extracts (Han In-jin, Artemisia iwayomogi kitamura and Cham Dang-gwi, Angelicae gigas Nakai) was absorbed into pellets and fed to whiteleg shrimp Litopenaeus vannamei for 12 weeks. The weight gain, immune activity, and disease resistance effects of the shirmp were examined. The growth performance of shrimp fed the mixture of medicinal herbal extracts was higher than that of the control group. The prophenoloxidase (proPO) activity was found to be significantly higher at 8 weeks in the 0.1% medicinal herb extract fed group. The cumulative mortality when artificially infected with AHPND pathogens commonly decreased in the 8 weeks fed group when compared with the control group. Furthermore, the RPS (relative percentage survival) was improved. This study confirmed that a mixture of medicinal herbal extracts has a positive effect on growth performance, innate immunity and disease resistance in shrimp. The optimum concentration of the medicinal herb extract for shrimp feed was 0.1% .

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.1
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• pp.103-114
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• 2006

Herbal mixture water extract of (Chrysanthemum morifolium, Portulaca oleracea, Sanguisorba officinalis, Sophora flavescens, Phellodendron amurense, Cnidium monnieri) which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis, and Propionibacterium acne. The growth of Stapylococcus epidermis, and Propionibacterium acne was inhibited completely by addition of 1.0% of the extract. Also in the present study we examined the mixture extract on compound 48/80 induced allergy and LPS induced cyclooxygenase-2(COX-2) gene expression in RAW 264.7 macrophage. The results indicated the ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 28-60%, and 48-72% , respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW 264.7 cells. The LPS-induced cytokine gene expression including IL-$1{\beta}$, TNF-$\alpha$, and IL-6 were dose-dependently suppressed by the mixture extract. Based on these results, it is concluded that the herbal mixture water extract can be applied to the acne and skin diseases therapy.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.127-127
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• 2018

Probiotics are microorganisms that have beneficial effects on the health of the host. The health promoting effect by probiotics influences suppressing harmful bacteria, prevention of constipation, blood cholesterol reduction and regulation of blood pressure. Prebiotics are used to promote the growth or activity of microorganisms. Synbiotics, which are a mixture of probiotics and prebiotics, synergize in the intestines by complementing each other. Synbiotics not only improves the viability of the probiotics while passing through the gastrointestinal tract, maintain intestinal homeostasis, but also regulate balance of harmful and useful bacterial growth. Glycine max Merr (GMM) has been widely used in Asian countries to treat cancer, obesity, oxidative stress and imbalanced immune diseases. In addition, it has been reported that dietary fiber-rich grains promote bowel movements and prevent constipation. In this study, we investigated the viability of LactobacillIus buchneri (L.buchneri) strains, known as lactic acid bacteria under conditions of gastric fluid and intestinal fluid to determine the suitability of L.buchneri as probiotics. The adhesion ability of L.buchneri to caco-2 cells was also confirmed. The present studies showed that GMM extract promoted the growth and activity of L.buchneri strains as prebiotics. Also, this results suggested that the mixture of L.buchneri and GMM extract can helps maintain intestinal health and healthy body as synbiotics and health functional food material.

• Korean Journal of Medicinal Crop Science
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• v.16 no.4
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• pp.225-230
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• 2008

Diabetes mellitus is the fifth leading cause of death among Koreans. Control of hyperglycemia and dyslipidemia is strongly correlated with decrease in risks for cardiovascular diseases, the most common and fatal diabetic complication. The effects of chronic feeding of a mixture of Chinese herbs on blood lipid profile were measured in an animal model of type 2 diabetes mellitus, db/db mice (C57BL/Ks). The Chinese herb mixture was composed of Panax ginseng C. A. Meyer,Astragalus membranaceus, Glycyrrhiza uralensis, Lycium chinense, Morus, Pueraria thunbergiana, Prunella vulgaris var. lilacina, Acanthopanax sessiliflorus, Schizandra chinensis, Scutellaria baicalensis, Dioscorea batatas, Polygonatum doratumvar. pluriflorum, Paeonia lactiflora, and Rehmannia glutinosa in a ratio of 1 : 0.7 : 0.4 : 0.7 :0.4 : 0.7 : 1.1 : 0.9 : 0.4 : 0.4 : 0.7 :0.7 : 0.9 : 0.9. Methanol extract of the Chinese herb mixture was tested for the inhibitory activity against yeast ${\alpha}$-glucosidase in vitro. The Chinese herb mixture extract inhibited ${\alpha}$-glucosidase by 25.2% at the concentration of 0.5mg/mL. Four weekold male db/db mice (n = 14) were fed AIN-93G semipurified diet or diet containing 10% powder of the Chinese herb mixture for 6 weeks after 1 week of adaptation period. Body weight (39.5 ${\pm}$ 1.6 g) and food intake (4.3 ${\pm}$ 0.6 g/day) of the Chinese herb group were not significantly different from those of the control group (40.4 ${\pm}$ 2.6 g and 4.5 ${\pm}$ 0.6 g/day). Consumption of Chinese herb mixture significantly decreased plasma glucose level (442.5 ${\pm}$ 36.0mg/dL) compared with the control group (489.8 ${\pm}$ 34.6 mg/dL, p < 0.05). Plasma cholesterol level (159.2 ${\pm}$ 18.4 mg/dL) of the Chinese herb group was significantly lower than that of the control group (185.4 ${\pm}$ 13.7 mg/dL, p < 0.05). Blood glycated hemoglobin (6.3 ${\pm}$ 0.8%) and plasma triglyceride levels (99.4 ${\pm}$ 15.0mg/dL) of the Chinese herb group were not significantly different from those of the control group (6.7 ${\pm}$ 0.7% and 108.8 ${\pm}$ 11.0mg/dL). Thus, the Chinese herb mixture could be useful in the treatment of diabetes and cardiovascular complications of diabetes.

• Natural Product Sciences
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• v.21 no.2
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• pp.98-103
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• 2015

Pueraiae Radix (PR), Pueratia Folium (PF) and Sorbus commixta (SC) mixture, namely GS-SP (PR (1)/PF (2)/SC (0.5): v/v/v) was developed as hangover-relieving elixir and its effects on alcoholic metabolism have been investigated. The enzymatic activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) of GS-SP was shown higher than those of single treatment with PR, PL, SC, and the positive control group (YM-808). The survival rate of mouse liver cell line NCTC clone 1469 in the presence of acetaldehyde was 30.6, 22.2, and 8.7% at the GS-SP dosage level of 50, 100, and 200 μg/mL respectively. Different concentrations of 50, 100 and 200 mg/kg of GS-SP showed efficient activity for ADH and ALDH than YM-808 in rat fed with 25% ethanol. The levels of blood alcohol and acetaldehyde after oral administration of 200 mg/kg of GS-SP showed efficient activity of 11.7% and 37% than those of YM-808. These results have been supported to the potential for GS-SP to serve as an excellent potential in providing hangover relief and liver protection.

• The Korea Journal of Herbology
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• v.29 no.2
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• pp.55-60
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• 2014

Objectives : The aim of this study was to evaluate whether herb mixture extract (HME) would affect allergic contact dermatitis induced by 1-chloro-2,4-dinitro-chlorobenzene (DNCB) in mice. For this, the level of blood IgE was identified. To evaluate anti-inflammatory effect of HME, we tested HMC-1 cells stimulated by PMA+A231867. Methods : In order to evaluate allergic contact dermatitis effects we observed HME on contact-hypersensitive skin of Balb/cmice induced by 0.5% DNCB and measured concentration of IgE in blood of Balb/c mice. In order to evaluate anti-inflammatory effect of cytokine expression we used the method of enzyme-linked immunosorbent assay. Results : We confirmed deep wounds, erosions, progress of keratinization and drop out of dead skin cells from Balb/c mice induced by 0.5% DNCB. We also observed a remarkable decrease in symptoms of atopic dermatitis in the group that received injection of 200mg/kg HME. In addition, in the measurement outcome for IgE concentration in blood, we confirmed that IgE concentration was increased by treatment with DNCB only, while it was markedly decreased by treatment with HME. We confirmed that cytokine expression decreased through enzyme-linked immunosorbent assay and pERK, pJNK, and pp38 also decreased through western blot test Conclusions : According to the above results, HME has some effect on alleviating symptoms of allergic contact dermatitis and has anti-inflammatory effects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.6
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• pp.844-850
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• 2013

In this study, the optimal extraction conditions for three medicinal herbs as functional sources against inflammatory and arthritic diseases were developed. Traditional medicinal herbs were screened for their inhibition of hyaluronidase (HAse) activity and nitric oxide (NO) synthesis. For the screening of anti-inflammatory properties, ethanolic extracts of 53 species of traditional medicinal herb were examined. We confirmed that Astragalus membranaceus (A.R.), Schisandra chinensis (S.F.), and Platycodon grandiflorum (P.G.) inhibit NO production. For extraction from all three herbs simultaneously, an ethanol concentration of 95%, a 1:2:1 mixture ratio, and at 50 rpm mixing speed, for over 12 h and at $30^{\circ}C$ was the best condition for optimal extract yield and NO inhibition effects. HAse inhibition from the three herb extraction was three fold higher than single samples. The ethanol extracts were fractionated with various solvents (n-hexane, chloroform, ethyl acetate, n-butanol, and water). The ethyl acetate-soluble fraction of the herb mixture showed the highest extract yield (13%) and NO inhibition effects (73%). In conclusion, this study provides experimental evidence that a mixture of P.G., A.R., and S.F. could be used as a source of antioxidant ingredients in the food industry.