The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of mixture extract on allergic contact dermatitis and anti-inflammatory

한방 복합추출물이 알레르기성 접촉피부염과 항염증에 미치는 영향

  • Jang, Young-Ah (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University)
  • 장영아 (대구한의대학교 화장품약리학과) ;
  • 이진태 (대구한의대학교 화장품약리학과)
  • Received : 2014.02.12
  • Accepted : 2014.03.24
  • Published : 2014.03.30
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Abstract

Objectives : The aim of this study was to evaluate whether herb mixture extract (HME) would affect allergic contact dermatitis induced by 1-chloro-2,4-dinitro-chlorobenzene (DNCB) in mice. For this, the level of blood IgE was identified. To evaluate anti-inflammatory effect of HME, we tested HMC-1 cells stimulated by PMA+A231867. Methods : In order to evaluate allergic contact dermatitis effects we observed HME on contact-hypersensitive skin of Balb/cmice induced by 0.5% DNCB and measured concentration of IgE in blood of Balb/c mice. In order to evaluate anti-inflammatory effect of cytokine expression we used the method of enzyme-linked immunosorbent assay. Results : We confirmed deep wounds, erosions, progress of keratinization and drop out of dead skin cells from Balb/c mice induced by 0.5% DNCB. We also observed a remarkable decrease in symptoms of atopic dermatitis in the group that received injection of 200mg/kg HME. In addition, in the measurement outcome for IgE concentration in blood, we confirmed that IgE concentration was increased by treatment with DNCB only, while it was markedly decreased by treatment with HME. We confirmed that cytokine expression decreased through enzyme-linked immunosorbent assay and pERK, pJNK, and pp38 also decreased through western blot test Conclusions : According to the above results, HME has some effect on alleviating symptoms of allergic contact dermatitis and has anti-inflammatory effects.

Keywords

References

  1. Cooper KD. Atopic dermatitis: Recent trends in pathogenesis and therapy. J Invest Dermatol. 1994 ; 102(1) : 128-38. https://doi.org/10.1111/1523-1747.ep12371746
  2. Leung DY, Boguniwicz M, Howll MD, Nomura I, Hamid QA. New insights into atopic drmatitis. J Clin Invest. 2004 ; 113 : 651-7. https://doi.org/10.1172/JCI21060
  3. Park CI. Study on the development of cosmtic emulsion cream for patients with atopic dermatitis using Scutellaria Baicalensis. Kor J Herbology. 2006 ; 21(2) : 47-53.
  4. Kang SY, Hue SH, Kim SI. Immunoologic aspects of hypersnsitivity diseas in Korea. Seoul J Medicine. 1978 ; 19 : 45-53.
  5. Jiang J, Yamaguchi T, Funakushi N, Kuhara T, Fan PS, Ueki R, Suto H, Kase Y, Ikeda S, Ogawa H. Oral Administration of Yokukansan Inhibits th Development of Atopic Dermatitis-like Lesions in Isolated NC/Nga Mice. J Dermatol Sci. 2009 ; 56(1) : 37-42. https://doi.org/10.1016/j.jdermsci.2009.07.003
  6. Koblenzer CS. Itching and the Atopic Skin. J Allerrgy Clin Immun. 1999 ; 104 : 109-13. https://doi.org/10.1016/S0091-6749(99)70052-7
  7. Mihara K, Kuratani K, Matsui T, Nakamura M, Yokota K. Vital Role of the Itch-scratch Respons in Development of Spontaneous Dermatitis in NC/Nga Mice. Br J Dermatol. 2004 ; 151(2) : 335-45. https://doi.org/10.1111/j.1365-2133.2004.06036.x
  8. Kim YH, Park YS. Effect of Scutellaria baicalensis Water Extract on AntioxidativeActivity and Epidermal Thickness in DNCB-.induced Allergic Contact Dermatitis Animal Model. J Korean Soc Food Sci Nutr. 2006 ; 35(5) : 543-8. https://doi.org/10.3746/jkfn.2006.35.5.543
  9. Galli SJ, Gordon JR, Wershil BK. Cytokine production by mast cells and basophils. Curr Opin Immunol. 1991 ; 3 : 865-72. https://doi.org/10.1016/S0952-7915(05)80005-6
  10. Galli SJ, Tsai M, Piliponsky AM. The development of allergic inflammation. Nature. 2008 ; 454 : 445-54. https://doi.org/10.1038/nature07204
  11. Azzolina A, Bongiovanni A, Lampiasi N. SubstanceP induces TNF-${\alpha}$ and IL-6 production through $NF{\kappa}B$ in peritoneal mast cells. Biochim BiophysActa. 2003 ; 1643(1-3) : 75-83.
  12. Bokemeyer D, Sorokin A, Dunn MJ. Multiple intracellular MAP kinase signaling cascades. Kidney Int. 1996 ; 49 : 1187-98. https://doi.org/10.1038/ki.1996.172
  13. Torres C, Francis MK, Lorenzini A, Tresini M, Cristofalo VJ. Metabolic stabilization of MAP kinase phosphatase-2 in senescence of human fibroblasts. Exp Cell Res. 2003 ; 290(2) : 195-206. https://doi.org/10.1016/S0014-4827(03)00309-4
  14. Tasaka K. Anti-allrgic drugs. Drugs Today. 1986 ; 22 : 101-133.
  15. Kim SN, Lee CM, Kim YC. Comparison in Antioxidant Activities of Water Extracts of Green Tea, White Tea and Black Tea. J Invest Cosmetol. 2012 ; 8(3) : 165-70. https://doi.org/10.15810/jic.2012.8.3.002
  16. Kim IH, Kim JH, Kim JW, Kim TH, No JS, Do JA, Hwang WK. New Pharmaceutical Botany. Seoul : Hak Chang Sa. 1998 : 281.
  17. Hirota A, Taki S, Yano M, Abe N. 1,1-Diphenyl-2-picrl-hydrazyl radical-scavnging compounds from soyban miso and antiproliferative activity of isoflavones from toward the cancer cell lines. Biosci Biotechnol Biochem. 2000 ; 64 : 1038-40. https://doi.org/10.1271/bbb.64.1038
  18. Sung KC. A study on the pharmaceutical characteristics & analysis of glycyrrhizin extract. J Kor Oil Chem Soc. 2006 ; 23(3) : 212-5.
  19. Kim TJ. Korea resources plants. Seoul : Seoul National University Pub. 1991 : 262.
  20. An DK. A Pictorial Book of the Korean Flora. Seoul : Kyohak Publishing. 2000 : 1239.
  21. Yamahara J. Behavioral pharmacology of berberine-type alkaloids. (1) Central depressive action of Coptidis rhizoma and its constituents. Nihon Yakurigaku Zasshi. 1976 ; 72(7) : 899-908. https://doi.org/10.1254/fpj.72.899
  22. A Yagi, T Kanbara, N Morinobu. The effect of tyrosinase inhibition for aloe. Planta Medica. 1986 ; 3981 : 517-9.
  23. Kim HY. Studies on anti-inflammatory of Chestnut inner shells. Graduate School Daegu Haany University. 2012 : 22-34.
  24. Kim HH, Kim DH. The effect of yunkyopaedo-sangamibang on allergic contact dermatitis. J Inst Oriental Med Semyung Univ. 2001 ; 3 : 67-80.
  25. Kwon, OS, Kim JT, Park IS, Ahn SH, Lee HP, Kim HH, Gang YH. The effect of yunkyopaedo-sangamibang allergic contact dermatitis. J Inst Oriental Med Semyung Univ. 1999 ; 8 : 77-91.
  26. Ishizaka K. Regulation of IgE synthesis. Ann Rev Immunol. 1984 ; 2 : 159-82. https://doi.org/10.1146/annurev.iy.02.040184.001111
  27. Sator PG, Schmidt JB, Honigsmann H. Comparison of epidermal hydration and skin surface lipids in heathy individuals and in patients with atopic dermatitis. J Am Acad Dermatol. 2003 ; 48 : 352-8. https://doi.org/10.1067/mjd.2003.105
  28. Kimber I, Basketter DA, Grberick GF, Dearman RJ. Allergic contact dermatitis. Int Immunopharmacol. 2002 ; 2 : 201-11. https://doi.org/10.1016/S1567-5769(01)00173-4
  29. Matsuda H, Watanabe N, Geba GP, Sperl J, Tsudzuki M, Hiroi J, Matsumoto M, Ushio H, Saito S, Askenase PW, Ra C. Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Ng mice. Int Immunol. 1997 ; 9 : 461-6. https://doi.org/10.1093/intimm/9.3.461
  30. Eun JS, Lee DH, Jeon YK, Kwon YA, Kwon J. Effect of Kamichungdieum on immune reaction. Kor J Orient Physiol Pathol. 2003 ; 18 : 1391-6.
  31. Averinou G, Goules AV, Stavropoulos PG. Atopic dermatitis: new immunoglogic aspcts. Int J Dermatol. 2008 ; 47 : 219-24. https://doi.org/10.1111/j.1365-4632.2008.03471.x
  32. Standr S, Steinhoff M, Schmelz M, Weisshaar E, Metze D, Luger T. Neurophysiology of Pruritus: Cutaneous Elicitation of Itch. Arch Dermatol. 2003 ; 139(11) : 1463-70.
  33. Bradding P, Robrts JA, Brittn KM, Montefort S, Djukanovic R, Mueller R, Heusser CH, Howarth PH, Holgate ST. Interleukin-4,-5, and-6 and tumor nerosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines. Am J Respir Cell Mol Biol. 1994 ; 10 : 471-80. https://doi.org/10.1165/ajrcmb.10.5.8179909
  34. Gordon JR, Burd PR, Galli SJ. Mast cells as a source multifunctional cytokines. Immunol Today. 1990 ; 11 : 458-64. https://doi.org/10.1016/0167-5699(90)90176-A
  35. Kim SJ, Lee EJ, Song YS, Jeong HJ, Le KM, Kim HR, Chae HJ, Shin TY, Kim YK, Hong SH, Kim HM. Cheongyol saseuptang inhibits production of TNF-alpha, IL-6 and IL-8 as well as NF-kappa B activation in human mast cells. J Ethnopharmacol. 2005 ; 97 : 83-8. https://doi.org/10.1016/j.jep.2004.10.018
  36. Kim HM, Park YA, Lee EJ, Shin TY. Inhibition of immediate-type allergic reation by Rosa davurica Pall. in a murine model. J Ethnophamacol. 1999 ; 67(1) : 53-60. https://doi.org/10.1016/S0378-8741(99)00013-6