• Asian Pacific Journal of Cancer Prevention
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• 제15권16호
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• pp.6983-6987
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• 2014

Background: Modified toluidine blue staining (MTBs) is a simple, inexpensive and time saving method to detect H. pylori in gastric biopsy specimens. As a metachromatic stain, it simultaneously highlights intestinal metaplasia, a gastric cancer precancerous lesion. The aim of this study was to assess the reliability of MTBs compared with hematoxylin-eosin (H&E) for H. pylori detection using immunoperoxidase staining as the gold standard. This technique would be beneficial for a routine diagnosis and confirmation of H. pylori eradication in developing countries where endoscopic-based approaches are dominant. Materials and Methods: Esophagogastroduodenoscopy with triple site gastric biopsies was undertaken in 207 dyspeptic patients at Thammasat University Hospital, Thailand between 1997 and 1999. H&E, MTBs and immunoperoxidase staining were applied to each specimen. The presence or absence of H. pylori with each stain was interpreted separately and the sensitivity, specificity, positive and negative predictive values of H&E and MTBs were calculated. Results: A total of 282 specimens from 207 patients were evaluated. Using immunoperoxidase staining, organisms were positive in 117 specimens (41%). MTBs proved almost equally sensitive as immunoperoxidase (99%) and significantly more sensitive than H&E (85%). It has comparable specificity (96% vs 96%), PPV (95% vs 94%), and NPV (99% vs 90%) to H&E, using immunoperoxidase staining as gold standard. MTBs compared with immunoperoxidase staining, is cheaper (2 USD vs 12 USD) and faster (20 min vs 16 hrs) compared to immunoperoxidase staining. Conclusions: MTBs is effective, economical and easy to use in daily practice for the detection of H. pylori in gastric biopsy specimens. In addition to saving time in evaluating H. pylori associated gastritis, with a high sensitivity and ability to demonstrate intestinal metaplasia, the technique may have a role in confirmation of H. pylori eradication for gastric cancer prevention in a developing country setting.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 춘계학술발표대회
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• pp.54.2-54.2
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• 2011

DTBP (dimethyl 3,3`-dithiobispropionimidate) was applied to collagen and gelatin coating on BCP granules and a crosslinking agent. The DTBP crosslinking was done for decreasing the solubility of the coating and hence increasing the stability. The nanostructure of collagen and gelatin coating surfaces were observed by SEM technique. Based on the DSC thermograms and FT-IR spectrums, the crosslinkings were confirmed between collagen molecules and gelatin molecules. The compressive strength was measured before crosslinking and after that. In-vitro study was carried out by measuring cell viability and observing cell morphology after DTBP crosslinking. Moreover, the proliferation ability of MG-63 osteoblast-like cells on the crosslinked BCP granules was evaluated by Western blot assay. The BCP granules were implanted into rabbit femur for 4 weeks and 12 weeks. The bone tissue formation was analyzed with micro-computed tomography (micro-CT) and histological analysis was also carried out by hematoxylin and eosin (H&E) staining for visualization of cells.

• Food Science and Biotechnology
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• 제16권4호
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• pp.605-609
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• 2007

The effects of lactic acid fermented yam yogurt (Yam/YG) on colon mucosal tissue were investigated in a loperamide-induced constipation rat models. Sprague-Dawley rats were fed for 6 weeks with 3 types of diets (normal, supplemented with lactic acid bacteria, and supplemented with Yam/YG), and were then administered loperamide intraperitoneally twice daily for 5 days. Administration of loperamide decreased fecal excretion and the moisture content of feces with increasing of numbers of pellets in the colon. On the histopathologic findings from hematoxylin and eosin (H& E) and alcian blue stainings, supplementation with Yam/YG resulted in the recovery of depleted goblet cells and mucin, and increased the numbers of Ki-67 positive cells, indicating restoration of colonic mucosa through cell proliferation and crypt regeneration against damages observed in crypt epithelial cells of loperamide-induced rats. These results indicate that Yam/YG improves evacuation and mucus production in the gastrointestinal tracts of constipated-induced rats.

• 시스템엔지니어링학술지
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• 제17권2호
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• pp.91-97
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• 2021

User interface for cancer classification system is a software application with clinician's friendly tools and functions to diagnose cancer from pathology images. Pathology evolved from manual diagnosis to computer-aided diagnosis with the help of Artificial Intelligence tools and algorithms. In this paper, we explained each block of the project life cycle for the implementation of automated breast cancer classification software using AI and machine learning algorithms to classify normal and invasive breast histology images. The system was designed to help the pathologists in an automatic and efficient diagnosis of breast cancer. To design the classification model, Hematoxylin and Eosin (H&E) stained breast histology images were obtained from the ICIAR Breast Cancer challenge. These images are stain normalized to minimize the error that can occur during model training due to pathological stains. The normalized dataset was fed into the ResNet-34 for the classification of normal and invasive breast cancer images. ResNet-34 gave 94% accuracy, 93% F Score, 95% of model Recall, and 91% precision.

• 대한융합한의학회지
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• 제5권2호
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• pp.17-22
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• 2023

Objectives: This study aimed to investigate the anti-atopic properties of Glechoma hederacea var. longituba extracts (GHE) in murine atopic dermatitis model. Methods: BALB/c mouse ear stimulated with oxazolone (OX) for 4 weeks, then 1% GHE was topically applied every two days for 3 weeks to mouse. Ear thickness was measured by a digital thickness gauge. The ears tissues were collected and subjected to hematoxylin-eosin (H&E) and toluidine blue (TB) staining. Results: Treatment with GHE successfully alleviated the symptoms of atopic dermatitis, such as erythema, horny substance, and swelling. The infiltration of lymphocytes and mast cells were significantly reduced following GHE treatment. Conclusion: Taken together, our results showed that GHE possessed the potential to be a novel immunomodulatory drug against atopic dermatitis.

• 환경생물
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• 제17권3호
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• pp.263-270
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• 1999

생쥐에 1 Gray(Gy)와 3 Gy의 방사선을 조사한 후 시간에 따라 골격근, 심장, 신장, 간 및 정소조직의 형태적 변화를 확인하였다. H-B(hematoxylin-eosin)염색 결과 apoptotic body는 다른 조직에 비해 간조직에서 쉽게 관찰되었고, 조사량에 비례하였으며 대부분의 조직에서 조사 후 1일에, 정소조직에서는 7일에 많이 관찰되었다. TUNEL (terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling)염색에 의한 결과도 동일하게 나타났다. 방사선 조사 후 단백질량은 신장을 제외한 나머지 조직에서 감소되었으며, 3 Gy를 조사한 경우 1 Gy보다 모든 조직에서 초기 2시간까지 감소되었으나 7일에는 모두 증가되었다. 젖산탈수소효소(EC 1.1.1.27, Lactate dehydrogenase: LDH) 활성은 모든 조직에서 증가되었고 1 Gy를 조사한 경우 초기에 더욱 증가되었으며 최고 활성은 3 Gy보다 빠른 시기에 나타났다. 골격근, 심장, 신장 및 정소조직은 일반적으로 $A_4$동위효소 활성이 감소되고 골격근과 심장은 $B_4$와 $A_2$$B_2$동위효소 활성이 증가되었으며 신장은 이질사량체의 활성이 증가되었다. 간은 $A_4$ 동위효소 활성이 높았고 정소는 C동위효소의 활성이 높았다. 따라서 LDH는 방사선 조사시 간조직을 제외한 나머지 조직에서 lactate oxidase로서 작용하여 호기적 대사가 우세한 것으로 사료된다.

• 대한의생명과학회지
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• 제17권3호
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• pp.197-202
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• 2011

Mitosis count is one of the most helpful morphologic features for distinguishing pulmonary typical carcinoid (TC) from atypical carcinoid (AC). However, identifying areas of highest mitotic activity is tedious and time-consuming, and mitosis count may vary substantially among pathologists. Anti-phosphohistone H3 (PHH3) is an antibody that specifically detects histone H3 only when phosphorylated at serine 10 or serine 28, an event that is concurrent with mitotic chromatin condensation and not observed during apoptosis. In this study, immunohistochemical staining for PHH3 was performed to determine whether PHH3 was a reliable and objective mitosis-specific marker for pulmonary carcinoid tumors. Seventeen cases of surgically resected pulmonary carcinoid tumors (12 TCs and 5 ACs) were obtained and classified according to the 2004 World Health Organization classification. Mitotic counts determined by PHH3 correlated to ones determined by hematoxylin and eosin (H&E) staining; however, PHH3 mitotic counts (mean mitotic counts: 1 in TCs and 3.2 in ACs) were slightly higher than H&E mitotic counts (mean mitotic counts: 0.25 in TCs and 1.8 in ACs). The mitotic counts determined by experienced observer were more correlated to those determined by inexperienced observer with the PHH3-based method (R=0.968, P<0.001) rather than H&E staining (R=0.658, P<0.001). These results suggest that the PHH3 mitotic counting method was more sensitive and simple for detecting mitoses compared to traditional H&E staining. Therefore, PHH3 immunohistochemistry may contribute to more accurate and reproducible diagnosis of pulmonary carcinoid tumors and may be a valuable aid for administrating appropriate clinical treatment.

• 대한세포병리학회지
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• 제17권1호
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• pp.32-37
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• 2006

The objective of this study was to evaluate the cytomorphologic features of histologically confirmed clear cell carcinoma of the ovary and to evaluate the applicability of scrape or fine-needle aspiration cytology in making an intraoperative diagnosis. We reviewed scrape or fine-needle aspiration cytology findings in tissues taken from 6 patients with clear cell carcinoma of the ovary. The cytologic diagnosis was based primarily on findings in alcohol-fixed, hematoxylin-eosin (H-E) stained smears. The formation of material resembling a basement membrane was a characteristic finding in these smears. This extracellular hyaline material was stained light pink with H-E and was frequently found within tumor cell clusters as well as in the background material. Multinucleated giant cells were found occasionally. Each tumor cell had an abundant, clear, or granular cytoplasm with a distinct cellular membrane. Scrape cytology is a simple and rapid supportive method and could be helpful in diagnosing clear cell carcinoma of the ovary, especially when marked artifacts appear in the frozen section.

• Natural Product Sciences
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• 제25권3호
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• pp.261-267
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• 2019

The rhizomes of Dioscorea japonica Thunb. are widely consumed as food and also used to treat diabetes and polyuria in Korea. This study was undertaken to study the anti-atopic dermatitis effects of a 95% ethanolic extract (DJE) of D. japonica in an oxazolone-stimulated murine model of atopic dermatitis (AD). The therapeutic effects of DJE on AD-like skin lesions were assessed on both ears. DJE (1%) or dexamethasone (0.5%; the positive control) were applied to skin lesions for three weeks. Serum levels of IgE and IL-4 were assessed by ELISA (enzyme-linked immunosorbent assay). Histopathological examinations were performed by hematoxylin and eosin (H&E) and toluidine blue staining and revealed DJE significantly reduced dermal thickness and inflammatory cell infiltration when applied to oxazolone-treated ear skin. DJE-treated AD mice also showed lower serum levels of IgE and IL-4 than oxazolone-stimulated controls. Our findings demonstrate DJE might be a useful safe, topical agent for the treatment of atopic diseases.

• 대한지역사회작업치료학회지
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• 제8권2호
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• pp.29-37
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• 2018

목적 : 수면장애는 노인에게 있어 다양한 이유로 빈발하고 있다. 이러한 노인의 수면장애에서 운동 강도에 따른 지속적인 운동습관이 수면장애의 치료적 효과를 줄 수 있는지를 밝히기 위해서 뇌파와 멜라토닌의 농도를 이용한 과학적인 방법으로 접근하는 것이 본 연구의 목적이다. 연구방법 : 일부지역의 노인시설에 거주하는 65세 이상의 노인 30명을 대상으로 하였다. 수면장애의 판단은 수면시간 6시간 이하의 경우(Kozier et al, 2004)와 PSQI(Pittsburgh Sleep Quality Index)의 점수를 동시에 만족하는 경우로 정하였다. 운동유형별 적용은 저강도의 걷기운동, 중강도의 유산소 운동, 고강도의 저항성 근력운동을 적용하였으며 측정도구는 뇌파에서 파형을 체크하기 위하여 QEEG 8-System(LAXTHA Inc. KOREA) 기기와 수면의 질을 검사하기 위해서 수면다원검사에 사용하는 Polysomnograpy (Compumedics, Australia) 기기를 사용하였다. 그리고 TTC(triphenyltetrazolium chloride) 염색과 H & E(Hematoxylin & Eosin) 염색을 통해 조직학적 양상을 관찰하였다. 결과 : 중강도의 유산소 운동 후에 멜라토닌의 농도와 뇌파로 측정한 수면지수에는 긍정적인 유의미한 변화가 있었다. 중강도의 유산소 운동에서는 SWS가 저강도, 고강도 운동보다 유의한 효과가 있었고 SWS의 관찰지표인 델타파의 유의한 변화가 있었다. 결론 : 인체의 일주기성 리듬의 생화학적, 행동학적 기능에 변화를 가져오는 노인에게서 수면장애에 대한 지속적인 운동은 운동의 유형에 따라 효과가 다름을 알 수 있었고 특히 유산소 운동이 다른 운동보다 노인 수면장애에 효과가 크다는 것을 멜라토닌의 농도와 뇌파를 통하여 알 수 있었다. 노인에게 맞춤형 운동을 제공하여 노인들의 삶의 질에 유용하고 건강한 말년을 제공할 수 있을 것이다.