• Journal of Sericultural and Entomological Science
• /
• v.29 no.1
• /
• pp.48-52
• /
• 1987

The fauna and the distribution of plant parasitic namatodes were investigated by smapling about five hundred grams of soil around roots of mulberry trees from 929 mulberry fields in Korea. Of fourteen genera detected on mulberry fields, Paratylenchus aciculus, P. projectus, P. morius, Helicotylenchus dihystera, Meloidogyne arenaria were found to be parasitic to mulberry. Among them Paratylenchus species were the most abundant in mulberry fields, but Tylenchorhynchus sp., Aphelenchus sp., Helicotylenchus sp., and Meloidogyne sp. were also observed in high frequency. Meloidogyne species were observed in high frequency in Jeonbuk, Gyeonggi and Jeonnam, Helicotylenchus sp., in Gyeongnam and Gyeonggi, Xiphinema sp., in Jeonbuk and Jeonnam, and Paratylenchus sp., in Gyeonggi, Gyeongbuk, Gyeongnam, and Jeonnam. The growth of mulberry was poor as the increase of density of Paratylenchus sp.

• Korean journal of applied entomology
• /
• v.21 no.3 s.52
• /
• pp.146-152
• /
• 1982

Survey on potato parasitic nematodes has been undertaken in order to find distribution of the nemic fauna. 41 soil samples were taken from potato fields in Gyeongbug, Gyeongnam and Gangweon provinces. Twenty four species belonging to 16 different genera were identified. Potato cyst nematode, Globodera rostochiensis was not found in the areas. Potato-rot nematode, Ditylenchus destructor and stem nematode, Ditylenchus dipsaci were found from several potato fields and population density high and showed damage to the crops. Root-lesion nematode, Pratylenchus minyus, Pratylenchus penetrans, Pratytenchus thornei and Pratylenchus vulnus were found and their population of these four species were high depending on the fields. Root-knot nematode, Meloidogyne hapla and Meloidogyne incognita were found. M. hapla was found only in Gangweon province and the population density was high. M. incognita was found at Milyang in Gyeongnam province. Spiral nematode, Heticotylenchus digonichus, Helicotylenchus dihystera, Helicotylenchus pseudorobustus, Rotylenchus orientalis and Rotylenchus pini were found. Aphelenchoides saprophilus, Criconemoides informis, Ditylenchus destructor, D. dipsaci, Helicotylenchus digonichus, H. dihystera, Hemicriconemoides intermedius, Meloidogyne hapla, Psilenchus hilarulus, Pratylenchus minyus, and Xiphinema americanum were first found from potato fields in Korea.

• Research in Plant Disease
• /
• v.20 no.2
• /
• pp.107-111
• /
• 2014

A greenhouse experiment was conducted to examine the effect of initial population density (Pi) of Helicotylenchus dihystera on six commercial tomato cultivars. Two-week-old tomato seedlings of six commercial cultivars were transplanted in d-10-cm clay pot and was inoculated with to give 0, 0.02, 0.2, and 2 nematodes/g soil. Plants were grown in a greenhouse for 60 days. Root and plant weights were unaffected but plant height declined only at inoculum level of 2 nematodes/g soil. At the highest initial population density (2 nematodes/g soil), plant height of tomato cv. Poseidon was reduced by 24%. Tomato cv. Hoyong produced the most nematodes with 7.0 nematodes/g soil and the least was tomato cv. Miniheuksu with 2.2 nematodes/g soil.

• Korean journal of applied entomology
• /
• v.42 no.2
• /
• pp.91-100
• /
• 2003

Helicotylenchus clarkei Sher, 1966 and Rotylenchus usitatus Van den Berg et Heyns, 1974 were newly found in Korea from medicinal plant collection field in Gyeongbuk Agricultural Technology Administration, Daegu, Korea. So far, 9 species of Rotylechus (R. usitatus) and 8 species of Helicotylenchus (H. belli, H. cavenessi, H. clarkei, H. digonicus, H. dihystera, H. erythrinae, H. paraplatyarus, H. pseudorobustus) are recorded in Korea.

• The Plant Pathology Journal
• /
• v.40 no.2
• /
• pp.171-191
• /
• 2024

Identification of Helicotylenchus species is very challenging due to phenotypic plasticity and existence of cryptic species complexes. Recently, the use of rDNA barcodes has proven to be useful for identification of Helicotylenchus. Molecular markers are a quick diagnostic tool and are crucial for discriminating related species and resolving cryptic species complexes within this speciose genus. However, DNA barcoding is not an error-free approach. The public databases appear to be marred by incorrect sequences, arising from sequencing errors, mislabeling, and misidentifications. Herein, we provide a comprehensive analysis of the newly obtained, and published DNA sequences of Helicotylenchus, revealing the potential faults in the available DNA barcodes. A total of 97 sequences (25 nearly full-length 18S-rRNA, 12 partial 28S-rRNA, 16 partial internal transcribed spacer [ITS]-rRNA, and 44 partial cytochrome c oxidase subunit I [COI] gene sequences) were newly obtained in the present study. Phylogenetic relationships between species are given as inferred from the analyses of 103 sequences of 18S-rRNA, 469 sequences of 28S-rRNA, 183 sequences of ITS-rRNA, and 63 sequences of COI. Remarks on suggested corrections of published accessions in GenBank database are given. Additionally, COI gene sequences of H. dihystera, H. asiaticus and the contentious H. microlobus are provided herein for the first time. Similar to rDNA gene analyses, the COI sequences support the genetic distinctness and validity of H. microlobus. DNA barcodes from type material are needed for resolving the taxonomic status of the unresolved taxonomic groups within the genus.

• Current Research on Agriculture and Life Sciences
• /
• v.1
• /
• pp.41-46
• /
• 1983

Nine species of plant parasitic nematodes, Aphelenchoides besseyi, Aphelenchus avenae, Criconemoides informis, Helicotylenchus dihystera, Meloidogyne arenaria, Meloidogyne hapla, Pratylenchus minyus and Tylenchus sp. Were found in peanut field in Korea. Chemicals used were; Telon C-17, Mocap 10G and Carbofuran 3G for control peanut parasitic nematodes. All chemicals reduced nematode populations but varied in effectiveness. Telon C-17 was especially effective against Meloidogyne hapla, the principal species on peanut and resulted in significant yield increased than the control. Plant height, number of branches and dry weight of peanut were increased over the nontreated control by chemical soil treatments.

• Korean journal of applied entomology
• /
• v.25 no.3 s.68
• /
• pp.175-182
• /
• 1986

A study was conducted to survey the fauna and the field density ofp lant parastic nematodes on important cash crops in Korea. Studies were carried out with sampling about five hundred grams of soil around roots of eighteen different kinds of crops from fifty five locations throughout the country. Nematodes were elutriated from over three thousand soil samples, and identified into sixteen different genera. Among them Ditylenchus species were found to be the most abundant in fields of garlic and onion, Helicoty-lenchus of sesame, chinese cabbage and mulberry, Meloidogyne of red-pepper, tomato, cucumber and peanut, Pratylenchus of mulberry and peach, Pratylenchus of apple, Chinese cabbage and radish, Trichodorus of potato, and Xiphinema of peach and potato. Field density of Meloidogyne species was increased with the longer continuous cultivation of red-pepper in the same field.

• The Plant Pathology Journal
• /
• v.21 no.4
• /
• pp.387-390
• /
• 2005

A survey was conducted during February-March 2003 to determine the occurrence and population density of plant-parasitic nematodes in strawberry (Fragaria ananassa) fields, cultivated under plastic houses in major strawberry growing regions of Gyeongbuk and Chonbuk provinces, Korea. The survey revealed presence of eleven species of plant-parasitic nematodes viz., Aphelenchoides fragariae, Criconemoides morgensis, Ditylenchus dipsaci, Helicotylenchus dihystera, Hirschmanniella imamuri, Meloidogyne arenaria, M. incognita, Pratylenchus penetrans, Psilenchus hilarulus, Tylenchorhynchus claytoni and Xiphinema sp. Frequency and density of each species were highly variable from field to field and within the field. Plant growth was not uniform in the surveyed fields under plastic houses; stunted growth, chlorotic leaves, small curled or crinkled leaves, deformed buds and flowers and wilted plants with fewer fruits were observed in patches.

• Korean Journal of Medicinal Crop Science
• /
• v.12 no.5
• /
• pp.355-359
• /
• 2004

A survey was conducted during $April{\sim}May$ 2004 to determine the occurrence and population density of plant-parasitic nematodes in ginseng (Panax ginseng C.A. Meyer) growing fields, in major ginseng growing regions of Chungbuk, Chungnam, Gyeongbuk and Kyongki provinces. The survey revealed presence of eleven species of plant-parasitic nematodes namely, Criconemoides morgensis, Ditylenchus destructor, Helicotylenchus dihystera, Meloidogyne incognita, M. hapla, Paratylencgus lepidus, Pratylenchus penetrans, Psilenchus hilarulus, Trichodorus similis, Tylenchorhynchus claytoni and Xiphinema americanum. Frequency and density of each species were highly variable. M. incognita and M. hapla were the predominant species, their infestation observed in 46.3 and 39.4% fields with an average density of $78{\sim}254\;and\;76{\sim}211$ nematodes per $300\;cm^3$ soil, respectively. Whereas, T. similis and X. americanum were rarely observed; only in 2.3 and 1.8% of surveyed fields and their density was $10{\sim}17\;and\;7{\sim}10$ individuals per $300\;cm^3$ soil, respectively. They are recorded herewith for the first time from ginseng fields of Korea. In nematode-infestated fields, stunted plant growth with chlorotic leaves, and wilted plants were observed in patches.

• Korean journal of applied entomology
• /
• v.15 no.3 s.28
• /
• pp.133-136
• /
• 1976

In order to survey the kinds and distribution of tobacco parasitic nematodes, 51 soil samples were. collected from 14 associations under the office of Monopoly of Kyungbuk and Kyungnam province. 1, The plant parasitic nematodes were found $96.1\%$ in the whole sampled. 2. The propotion of infested field of each nematode genera, Tylenchus shown as dominant by $82.3\%$ and next Aphelenchus $72.5\%$, Tylenchorhynchus $58.8\%$, Meloidogyne$50.9\%$, Criconemoides $25.4\%$, Pratylenchus, Hirschmanniella $23.5\%$ in order. 3. The density of genera per localities were as follows Pratylenchus is most abundant in Youngdeog as 43 population, Tylenchorhynchus shown in Jinbo, Tylenhus in Yeongil, Meloidogyne in Kimcheon, Xiphinema in Euisung. 4. Eighteen species belonging to 16 different genera of 11 families were identified, 12 of them are new for Korea as tobacco parasitic nematodes; Aglenchus costatus, Aphelenchus avenae, Criconemoides informis, Ditylenchus dipsaci, Helicotylenchus dihystera Hirschmanniella imamuri, Meloidogyne incognita, Psilenchus hilarulus, Rotylenchus pini, Tylenchorhynchus claytoni, Tylenchorhynchus nudus and Xiphinema americanum.