• Title/Summary/Keyword: Helianthus annuus L.

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Simultaneous Quantitative Analysis of Major Constituent of Ethanol Extract from Leaves of Helianthus annuus L. (향일규 잎의 에탄올 추출물에 대한 주요 성분 동시 정량분석)

  • Kim, Eun-Nam;Jeon, Sang-Young;Jeong, Gil-Saeng
    • Korean Journal of Pharmacognosy
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    • v.52 no.2
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    • pp.112-117
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    • 2021
  • Helianthus annuus L. has been reported with various pharmacological activities such as antibacterial, antidiabetic, and antioxidant effects. According to recent studies, H. annuus L. is known to contain components such as phenolic compounds, flavonoids, alkaloids, sesquiterpenoids, and lignans. The seeds of H. annuus L. have been reported to contain chlorogenic acid and di-O-caffeoylquinic acid as major components. However, studies on the main components and content of leaves of H. annuus L. are still incomplete. Therefore, in this study, the contents of four major components of H. annuus L. were evaluated by simultaneous quantitative analysis with high performance liquid chromatography (HPLC)-diode array detector (DAD). The isolated four compounds Caffeoylquinic acid(CQA), 3,4-dicaffeoylquinic acid(3,4-DCQA), 3,5-dicaffeoylquinic acid(3,5-DCQA) and 4,5-dicaffeoylquinic acid(4,5-DCQA) were shown in a large linearity with a correlation coefficient (R2) of 0.99. In addition, as a result of intra-inter day analysis of four major compounds by the analysis method of this study, the accuracy of 88.46% or more and less than 112.85% and excellent precision of less than 3% were shown, the content analysis showed CQA (0.383±0.018 mg/g), 3,4-DCQA (0.282±0.017 mg/g), 3,5-DCQA (1.109±0.068 mg/g), and 4,5-DCQA (0.673±0.020 mg/g).

The origin of the word of sunflower (해바라기(향일규(向日葵), 향일화(向日花))의 어원(語源)에 대하여)

  • Kim, Jong-Dug;Koh, Byung-Hee
    • Korean Journal of Oriental Medicine
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    • v.7 no.1
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    • pp.55-66
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    • 2001
  • According to the customary, naming is done after the subject is in existence. But the name's Hae-ba-ra-gi(해바라기), 향일규(向日葵), 향일화(向日花))' has been used as an alias of Hibiscus manihot L.(닥풀) long before Helianthus annuus L.(sunflower) was brought in to Korea. And now the usage of the name has been conversed since them. Since the incorrect record of '葵藿' and '葵花'as '해바라기' in ${\ulcorner}$조선어사전(朝鮮語辭典)${\lrcorner}$(1920), the mistake has been carried on this must be corrected from now on. Incorrect record of hollyhock(蜀葵花) '해바라기' in ${\ulcorner}$몽유(蒙喩))${\lrcorner}$(1810) took a role in this incorrect trend.

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Molecular Cloning of a cDNA Encoding Ribulose-1,5-bisphosphate Carboxylase Small Subunit (rbcS) from Panax ginseng C. A. Meyer (고려인삼의 Ribulose-1,5-Bisphosphate Carboxylase Small Subunit(rbcS) 유전자의 분리 및 특성분석)

  • In Jun Gyo;Lee Bum Soo;Youn Jae-Ho;Son Hwa;Yi Tae Hoo;Yang Deok Chun
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.374-381
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    • 2005
  • A full-length cDNA encoding ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) has been isolated and its nucleotide sequence determined from root in ginseng plant (Panax ginseng). The rbcS cDNA of ginseng is 790 nucleotides long and has an open reading frame of 549 bp with deduced amino acid of 183 residues (pI 8.37), 20.5 kDa. The deduced amino acid sequence of rbcS matched to the previously reported rbcS protein genes and showed a high similarity with the $78\%$ identity with rbcS of Helianthus annuus (CAA68490). In the phylogenetic analysis based on the amino acid residues, the ginseng rbcS was clustered with H. annuus (CAA68490), C. morifolium (AA025119) and L. sativa (Q40250).

Rhizofiltration Process with Helianthus annuss L., Phaseolus vulgaris var., and Brassica juncea (L.) Czern. to Remediate Uranium Contaminated Groundwater (해바라기, 갓, 강낭콩을 이용한 수생법(Rhizofiltration)의 우라늄으로 오염된 지하수 정화 효율 규명)

  • Yang, Min-June;Lee, Min-Hee
    • Journal of Soil and Groundwater Environment
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    • v.13 no.4
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    • pp.30-39
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    • 2008
  • The uranium removal efficiency of rhizofiltration was investigated in lab scale experiment. Three plants such as sunflower (Helianthus annuus L.), bean (Phaseolus vulgaris var.), and Indian mustard (Brassica juncea (L.) Czern.) were cultivated in artificially contaminated solution by uranium at 30 ${\mu}g$/L and 80 ${\mu}g$/L for 72 hours. The removal efficiencies of three cultivars were calculated from the ICP/MS analysis of uranium mass in solution and the plant. For Helianthus annuus L., more than 80% of initial uranium were removed from the solution and the uranium concentration of residual solution maintained lower than 10 ${\mu}g$/L. For Phaseolus vulgaris var. and Brassica juncea (L.) Czern., their uranium removal efficiencies ranged from 60 to 80%. Even the uranium concentration of solution was higher than 500 ${\mu}g$/L, these cultivars removed more than 70% of initial uranium by rhizofiltration, suggesting that the rhizofiltration has a great capability to remove uranium in the contaminated groundwater. The removal efficiency of rhizofiltration by using Brassica juncea (L.) Czern decreased from 83% to 42% with the increase of pH in solution. From the results of the analysis for the uranium accumulation in plants, 99% of uranium transferred into the plant from solution were accumulated in the root and only 1% of uranium existed in the shoot part (including leaves), suggesting that the cost and the time to treat massive grown plants after the rhizofiltration could be dramatically cut down because only their root parts needs to be treated. Finally, the genuine groundwater having high uranium concentration (81.4 ${\mu}g$/L), sampled from Daejoun area, was used in the experiment. The uranium removal efficiency of Helianthus annuus L. for the real groundwater was higher than 95%, investigating that the rhizofiltration is the very useful method to remediate uranium contaminated groundwater.

Development of High Throughput Screening Techniques Using Food-borne Library against Anti-asthma Agents (식품소재 라이브러리를 이용한 천식 완화용 물질의 초고속스크리닝 기법 개발)

  • Heo Jin-Chul;Park Ja-Young;Kwon Taeg Kyu;Chung Shin Kyo;Kim Sung-Uk;Lee Sang-Han
    • Food Science and Preservation
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    • v.12 no.3
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    • pp.267-274
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    • 2005
  • Oxidant stress is a well-known pivotal parameter for the degenerative immune diseases including asthma, atopic dermatitis, and rhinitis. In order to screen for anti-asthma agents effectively, we first established the infrastructure of high throughput screening(HTS) for anti-oxidant agents from agricultural products and/or oriental medicine library extracted with water, methanol, dimethyl sulfoxide, ethyl acetate and juice, Using the screening system, we found that Chaenomelis langenariae, Rhus javanica L., Camellia sinensis, Helianthus annuus and Angelica utilis Makino had strong anti-oxidant activity. Moreover, Helianthus annuus, Rehmannia glutinosa Libo and Angelica utilis Makino have protection activities by treatment of an oxidant hydrogen peroxide. Together, these results suggest that screened agents could be potential agents against asthma, although the in vivo studies should be clearly tested.

Analysis of Quality Change during Preservation by a Prototype Drink Containing a Helianthus annuus Seed Extract (해바라기씨 추출물 함유 음료의 저장기간 중 품질 특성 분석)

  • Lee, Jin-Man;Kang, Bok-Hee;Lee, Sang-Han
    • Food Science and Preservation
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    • v.16 no.2
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    • pp.299-303
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    • 2009
  • To explore the preservation parameters of a prototype anti-inflammatory drink containing a Helianthus annuus seed extract, we first examined sweetness, pH and color changes, and temperature effects during preservation. Over 25 days, pH rose slightly from 3.50 to 3.65. Small color changes in L, a, and b values occurred on prolonged storage. Temperature most affected storage properties, which differed considerably at $10^{\circ}C$ and $40^{\circ}C$. Microbiological safety tests showed that the prototype drink was safe; neither general bacterial contamination nor Escherichia coli growth occurred. We concluded that the prototype drink was biologically and microbiologically safe on preservation.

Phytoextraction of Heavy Metals Induced by Bioaugmentation of a Phosphate Solubilizing Bacterium

  • Arunakumara, K.K.I.U.;Walpola, Buddhi Charana;Song, Jun-Seob;Shin, Min-Jung;Lee, Chan-Jung;Yoon, Min-Ho
    • Korean Journal of Environmental Agriculture
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    • v.33 no.3
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    • pp.220-230
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    • 2014
  • BACKGROUND: Excessive metals in the soil have become one of the most significant environmental problems. Phytoremediation has received considerable attention as a method for restoring the contaminated soils. The microbes having remarkable metal tolerance and plant growth-promoting abilities could also play a significant role in remediation of metal-contaminated soils, because bioaugmentation with such microbes could promote phytoextraction of metals. Therefore, the present study was focused on evaluating the phytoextraction of heavy metals (Co, Pb and Zn) in Helianthus annuus (sunflower) induced by bioaugmentation of a phosphate solubilizing bacterium. METHODS AND RESULTS: A phosphate solubilizing bacterium was isolated from metal-contaminated soils based on the greater halo size (>3 mm) with solid NBRIP agar medium containing 10 g glucose, 5 g $Ca_3(PO_4)_2$, 5 g $MgCl_2{\cdot}6H_2O$, 0.25 g $MgSO_4.7H_2O$, 0.2 g KCl, 0.1 g $(NH_4)_2SO_4$ in 1 L distilled water. Isolated bacterial strain was assessed for their resistance to heavy metals; $CoCl_2.6H_2O$, $2PbCO_3.Pb(OH)_2$, and $ZnCl_2$ at various concentrations ranging from $100-400{\mu}g/mL$ (Co, Pb and Zn) using the agar dilution method. A pot experiment was conducted with aqueous solutions of different heavy metals (Co, Pb and Zn) to assess the effect of bacterial strain on growth and metal uptake by Helianthus annuus (sunflower). The impact of bacterial inoculation on the mobility of metals in soil was investigated under laboratory conditions with 50 mL scaled polypropylene centrifuge tubes. The metal contents in the filtrate of plant extracts were determined using an atomic absorption spectrophotometer (Perkinelmer, Aanalyst 800, USA). CONCLUSION: Inoculation with Enterobacter ludwigii PSB 28 resulted in increased shoot and root biomass and enhanced accumulation of Co, Pb and Zn in Helianthus annuus plants. The strain was found to be capable of promoting metal translocation from the roots to the shoots of H. annuus. Therefore, Enterobacter ludwigii PSB 28 could be identified as an effective promoter of phytoextraction of Co, Pb and Zn from metal-contaminated soils.

A Case Study on Phytoremediation in Polluted Stream by Heilianthus annuus (Heilianthus annuus에 의한 오염된 하천에서의 Phytoremediation에 관한 연구)

  • Choi, Moon-Sul
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.11 no.4
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    • pp.165-171
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    • 2006
  • This is the research to prepare a purification program for relatively less polluted stream by using phytoremediation. We calculated a treatment amount of nutrients followed by growth of Helianthus annuus (a kind of sunflower), setting up the plant reactor in the hothouse. Moreover, to investigate a field applicability, we could find increased contents of nitrogen, carbon and hydrogen in plants by setting up a H. annuus planted artificial floating island in an irrigation canal. When we changed the dissolved inorganic nitrogen(DIN) concentration of the influent from 28.5 to 199.2 mg/l and the dissolved inorganic phosphorus(DIP) concentration of the influent from 13.3 to 25.4 mg/l, growth disorder has not appeared though it is much higher than the criterion of water for irrigation. In this case, the removal rate of DIN was $81.7\sim98.6%$, and that of DIP was $81.9\sim98.4%$ in 3 days stay on average. It has appeared that the efficient hydraulic retention time(HRT) was 48 hours. The following contents of nitrogen, carbon and hydrogen of H. annuus appeared in the artificial floating island: nitrogen was $3.2\sim7.8%$ in the trunk and $3.0\sim6.3%$ in the root. Carbon was $40.1\sim57.7%$ in the trunk and $43.4\sim53.8%$ in the root.

Cesium Removal of the Rhizofiltration Using Sunflowers (Helianthus annuss L.) and Beans (Phaseolos vulgaris var.) (해바라기(Helianthus annuss L.)와 강낭콩(Phaseolos vulgaris var.)을 이용한 뿌리여과법(rhizofiltration)의 세슘 (cesiun) 제거)

  • Yang, Min-June;Lee, Min-Hee
    • Economic and Environmental Geology
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    • v.41 no.6
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    • pp.709-717
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    • 2008
  • Rhizofiltration for cesium uptake by sunflowers (Helianthus annuus L.) and beans (Phaseolus vulgaris var.) was investigated for groundwater contamination. The cesium removal by sunflowers was greater than 98% of the total cesium in solution, and the uptake by beans was also greater than 99% within 24 hours of the rhizofiltration, showing that the rhizofiltration has a great capability to remove cesium from the contaminated water system. Experiments at various pH of solution indicated that a solution of pH $5{\sim}9$ yielded very high cesium accumulation in two plants. From the results of the analysis for cesium accumulation in plant parts, about 80% of cesium transferred into the plant from solution was accumulated in the root part and less than 20% of cesium existed in the shoot part (including leaves). Results suggest that only the roots of the fully grown plant used for rhizofiltration should be disposed or post-treated and thus the cost and time to treat massive amounts of grown plants could be dramatically reduced when sunflower and bean are used in the real field. The results of SEM and EDS analyses indicated that the most of cesium were accumulated in the root surface as a ionic phase rather than a soil precipitation phase.

Effects of Different Planting on Plant Height, Number of Leaves, Flowering and Yield on Sunflower (Helianthus annuus. L.) (해바라기에 있어서 파종기 이동이 초장, 엽수, 개화기 및 수량에 미치는 영향)

  • Kwang-He Kang;Eun-Woong Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.2
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    • pp.98-103
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    • 1977
  • To layout the pattern in ecological and productive traits of sunflower (Helianthus annuus L.), two cultivars, Peredovic and Gunpo-local, were grown at 6 planting dates from May 15 to July 25 by 20 day intervals. Delaying of planting dates, Cv. Peredovic showed less variation in days to reach the head-visible stage than Cv. Gunpo-local. and two varieties are different in photosensitivity. Plant height and stem diameter and number of leaves of Cv. Gunpo-local were greater than those of Cv. Peredovic at the early planting, but the differences were decreased as planting were delayed. Cv. Peredovic yielded 158kg achences per 10are relatively from the May 5's planting. However delaying of planting dates after July 5, there was serious reduction in the yield.

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