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Molecular Cloning of a cDNA Encoding Ribulose-1,5-bisphosphate Carboxylase Small Subunit (rbcS) from Panax ginseng C. A. Meyer  

In Jun Gyo (Research Institute of Biotechnology, BioPia Co., Ltd.)
Lee Bum Soo (Research Institute of Biotechnology, BioPia Co., Ltd.)
Youn Jae-Ho (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
Son Hwa (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
Yi Tae Hoo (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
Yang Deok Chun (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
Publication Information
Korean Journal of Plant Resources / v.18, no.3, 2005 , pp. 374-381 More about this Journal
Abstract
A full-length cDNA encoding ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) has been isolated and its nucleotide sequence determined from root in ginseng plant (Panax ginseng). The rbcS cDNA of ginseng is 790 nucleotides long and has an open reading frame of 549 bp with deduced amino acid of 183 residues (pI 8.37), 20.5 kDa. The deduced amino acid sequence of rbcS matched to the previously reported rbcS protein genes and showed a high similarity with the $78\%$ identity with rbcS of Helianthus annuus (CAA68490). In the phylogenetic analysis based on the amino acid residues, the ginseng rbcS was clustered with H. annuus (CAA68490), C. morifolium (AA025119) and L. sativa (Q40250).
Keywords
Panax ginseng; rbcC; ribulose-1,5-bisphosphate carboxylase; root;
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