• Title/Summary/Keyword: Hee-Uk Yang

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Aphicidal Activity of Starfish (Asterina pectinifera) Extracts against Green Peach Aphid (Myzus Persicae) (불가사리 추출물의 복숭아혹진딧물(Myzus Persicae) 살충활성)

  • Jang, Ja-Yeong;Yi, Sol;Jung, Iee-Young;Choi, Eun-Hyun;Jo, Uk-Hee;Seo, Jung-Mi;Yang, Si-Young;Kim, In-Seon
    • Korean Journal of Environmental Agriculture
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    • v.30 no.2
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    • pp.173-178
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    • 2011
  • BACKGROUND: Starfish is one of major sea invertebrates that have become a serious economic threat to aquacultural farms in Korea. Much effort has sacrificed to reduce the economic losses of the farms by predatory starfish, including developing and searching biological resources for medicinal and agricultural purposes. In the present study, we investigated aphicidal activity of the extracts from the starfish Asterina pectinifera against green peach aphid. METHODS AND RESULTS: Fresh starfishes were cut into small pieces, homogenized and soaked in methanol. The methanol extracts were centrifuged and the resulting supernatant was subjected to aphicidal activity assays and a series of silica gel column chromatography. More than 70% mortality of aphids were observed by the extracts at a concentration of 1,000 mg/L, exhibiting dose-dependent mortality. TOF-MS analyses detected polyhydroxysteroid as a main aphicidal compound from the starfish extracts. Transmission electronic microscopy could demonstrate that the extracts with polyhydroxysteroid caused aphids death by affecting their epicuticular membrane. CONCLUSION(s): This is the first report of aphicidal activity of the starfish Asterina pectinifera extracts against green peach aphid. Starfish biological resources may be used as a potential candidate for developing a new type natural insecticide.

Estimation of THI Index to Evaluate Thermal Stress of Piglets in Summer Season (하절기 자돈 고온 스트레스 평가를 위한 THI 지수 모의)

  • Ha, Taehwan;Kwon, Kyeong-seok;Lee, In-bok;Kim, Rack-woo;Yeo, Uk-hyeon;Lee, Sangyeon;Choi, Hee-chul;Kim, Jong-bok;Lee, Jun-yeob;Jeon, Jung-hwan;Woo, Saemee;Yang, Ka-young
    • Journal of The Korean Society of Agricultural Engineers
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    • v.60 no.4
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    • pp.113-122
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    • 2018
  • Thermal stress of pigs causes decreased feed consumption and weight gain rate, immunosuppression, reproductive disorders, and increased mortality. The concept of the temperature-humidity index (THI) has been widely used to evaluate the degree of thermal stress of pigs. However, use of this concept is strongly restricted for animals living in the enclosed facilities. In this study, Building Energy Simulation (BES) technique was used to realize the energy flow among outside weather conditions, building materials, and animals. Especially, mechanisms of sensible and latent heat generation from pigs according to surrounding air temperature and their weight were designed to accurately evaluate the THI values inside the pig house. The THI values computed by the BES model were compared to those calculated by method of the report (NIAS, 2016), the model of this study predicted the start date of heat stress about 9~76 days earlier compared to the NIAS model. Results of the BES model also showed higher frequencies of the THI above the THI threshold for pigs, indicating that conventional model has a possibility of underestimating the degree of heat stress of pigs.

Characterization of an Antimicrobial Substance-producing Pseudomonas sp. BCNU 2001 (항생물질을 생산하는 Pseudomonas sp. BCNU 2001 균주의 특성)

  • Yang, Uk-Hee;Choi, Hye-Jung;Ahn, Cheol-Soo;Jeong, Yong-Kee;Kim, Dong-Wan;Joo, Woo-Hong
    • Microbiology and Biotechnology Letters
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    • v.38 no.3
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    • pp.255-262
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    • 2010
  • Strain BCNU 2001 was isolated from soil samples collected from Tea-baek Mountain area. The biochemical characteristics and 16S ribosomal RNA gene sequences of the isolate revealed that the strain belonged to the Pseudomonas aeruginosa. The supernatants had an antimicrobial effect on various kind of bacteria and fungi. Especially BCNU 2001 was able to greatly inhibit the growth of Micrococcus luteus, Proteus mirabilis, Proteus vulgaris, and Aspergillus niger, and its inhibition zone was measured as 18.5 mm against Micrococcus luteus, 19.0mm against Proteus mirabilis, 17.0mm against Proteus vulgaris, and 13.5 mm against Aspergillus niger, respectively. Hexane and dichloromethane extracts of BCNU 2001 exhibited significant activity against bacteria, and dichloromethane and ethylacetate extracts showed significant activity against fungi. Pseudomonas strain BCNU 2001 was also determined to have antimicrobial peptide against various microorganisms including Gram positive bacteria, Gram negative bacteria and fungi. The obtained results may provide preliminary support for the usefulness of Pseudomonas strain BCNU 2001.

Synthesis of Defective-Structure Li4Mn5O12 by Combustion Method and Its Application to Hybrid Capacitor (연소합성법에 의한 결함구조 Li4Mn5O12제조와 하이브리드 커패시터 적용)

  • Kim, Hun-Uk;Sun, Yang-Kook;Lee, Bum-Suk;Jin, Chang-Soo;Shin, Kyoung-Hee
    • Journal of the Korean Electrochemical Society
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    • v.13 no.2
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    • pp.103-109
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    • 2010
  • $Li_4Mn_5O_{12}$ was synthesized by combustion method using $LiNO_3$, $Li(CH_3COO){\cdot}2H_2O$ and $Mn(CH_3COO)_2{\cdot}4H_2O$. $Li_4Mn_5O_{12}$ was obtained over $400^{\circ}C$, however, the sample calcined at $400^{\circ}C$ for any time was mixed phases of $Li_4Mn_5O_{12}$ and $Mn_2O_3$. $Li_4Mn_5O_{12}$ calcined at $400^{\circ}C$ for 5 h had larger first discharge capacity (41.5mAh/g) at 1C-rate for 3.7~4.4V than other calcined samples. Moreover, applying to hybrid capacitor, it had good discharge capacity (24.74 mAh/g or 10.46 mAh/cc) at 100 mA/g for 1~2.5 V and higher energy density (39Wh/kg or 16.49Wh/cc) at same condition.

The effect on balance and gait when applying Kinesio Taping to the lower extremities of hemiplegic patients (편마비 환자의 양쪽 하지 테이핑 적용이 균형 및 보행에 미치는 영향)

  • Park, Chang-Hee;Bae, Hwa-Suk;Kim, Hye-Young;Park, Hyon-Sun;Park, Hye-Seon;Jeong, Byeong-Uk;Choi, Jin-Suk;Seo, Han-Keong;Park, Hye-Jin;Kim, Myung-Hoon;Kim, Sang-Yup;Jung, Dae-In;Kim, Chan-Kyu;Yang, Jin-Hong;Kim, Hyun-Jin
    • Journal of Korean Physical Therapy Science
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    • v.18 no.1
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    • pp.11-19
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    • 2011
  • Purpose: The purpose of this study is to understand the effect of balance and gait when simultaneous application of Kinesio Taping on the lower extremities is done to improve the pattern of hemiplegic patients asymmetric lower extremity. Methods: This study was performed with 7 patients who have hemiplegia symptoms due to stoke. The taping on the paralyzed side was done on tibialis anterior, gluteus medius, gluteus maximus and psoas major and the taping on the unparalyzed side was done on the calf muscle and quadriceps. Berg Balance Scale(BBS) and Time Up and Go Test(TUG) was used to assessment the balance and gait of the patient after the kinesio taping was done on the lower extremities. The results of the test was analyzed by Wilcoxon Signed Rank Test on SPSS(Ver. 10.1). Results: When the taping was done on the paralyzed side, there was a significant improvement of balance and gait in BBS and TUG. When the taping was done on the lower extremities, it showed significant improvement of balance and gait, compared to when the taping was only done on the paralyzed side, according to BBS and TUG. Conclusion: The result of this study shows that the simultaneous taping of the lower extremities of the hemiplegic patients improves balance and gait by improving the pattern of patients lower extremities.

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Assessment of Leaching Potential for Pesticides Registered in Korea (국내등록농약의 용탈 가능성 평가)

  • Kim, Chan-Sub;Lee, Hee-Dong;Ihm, Yang-Bin;Kim, Jeong-Han;Im, Geon-Jae;Oh, Byung-Youl
    • The Korean Journal of Pesticide Science
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    • v.10 no.4
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    • pp.272-278
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    • 2006
  • Movement of pesticides applied for crop protection to the non-target environmental compartments has increasingly concerned in recent. A special review on groundwater leaching potential of pesticides registered in Korea was done by using the data submitted on half-life and adsorption of the pesticides in/on soil. Groundwater ubiquity score(GUS) of pesticides as a leaching potential was calculated by using domestic trial data on pesticide half-life in paddy or upland soils and Koc data bases of Oregon State University (OSU), British Pesticide Safety Directorate and Sweden. Of total 382 pesticides reviewed, domestic half-lives of 107 pesticides were for paddy soil and 297 pesticides for upland soil. And total 317 Koc values were collected 148 pesticides from OSU DB and 276 pesticides from UK/Sweden DB. Very highly leachable pesticides were 18 and highly leachable pesticides were 44 among 313 pesticides classified by GUS.

A Study of Marine Response system for the tar type waste oil (타르성 기름찌꺼기 해상방제 방안에 관한 연구)

  • Jang, Duck-Jong;Kim, Tae-Ho;Yang, Kyung-Uk;Na, Sun-Cheol;Nam, Kwang-Hee
    • Proceedings of KOSOMES biannual meeting
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    • 2008.05a
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    • pp.117-120
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    • 2008
  • As we can see at the oil spill occident on the coast of Taean, the viscosity of the spilled oil becomes thicker as time goes by. It becomes waste oil with the form of tar. It moves to other areas following a tide. When the temperature of the water goes up, the viscosity becomes lighter and forms oil film. It repeats the process spreading to and polluting extensive areas where the tide reaches. People have used hand nets to collect waste oil of tar at the sea. But it is very difficult for them to collect the tar type waste oil spread on large areas before it reaches to the beach. This paper tried to find a way to collect the tar type waste oil efficiently. It used absorption mat of boom type that uses the attachment characteristics of the tar type waste oil and floating waste recovery device of net type. It tested the possibility of using the devices at the oil spill accident on the beach of Taean. The result showed that net type recovery device was much more efficient in collecting the waste oil in short time than the hand net system of people. It confirmed that the net type recovery device could be used to collect tar type waste oil.

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Evaluation of thermal stress of poultry according to stocking densities using mumerical BES model (BES 수치모델을 이용한 사육 밀도별 가금류 고온 스트레스 평가)

  • Kwon, Kyeong-seok;Ha, Tahwan;Choi, Hee-chul;Kim, Jong-bok;Lee, Jun-yeob;Jeon, Jung-hwan;Yang, Ka-young;Kim, Rack-woo;Yeo, Uk-hyeon;Lee, Sang-yeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.1
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    • pp.456-463
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    • 2019
  • Micro climatic conditions within the livestock facility are affected by various factors such as ventilation, cooling, heating, insulation and latent and sensible heat generation from animals. In this study, numerical BES method was used to simulate energy flow inside the poultry house. Based on the BES method and THI concept, degree of thermal stress of poultry was evaluated according to the locations in South Korea. Comparison of THI values within the poultry house was also carried out according to the stocking densities to reflect recent animal-welfare issue. Significant decrease in thermal stress of poultry was observed when the stocking density of $30kg/m^2$ was applied in the change of the seasons(p<0.05) however, there was no statistically significant difference in summer season(p>0.05). It meant that installation of proper cooling system is urgently needed. For Iksan city of Jeollabuk-do province, total 252 hours of profit for thermal stress was found according to decrease in the stocking density.

Validation of QF-PCR for Rapid Prenatal Diagnosis of Common Chromosomal Aneuploidies in Korea

  • Han, Sung-Hee;Ryu, Jae-Song;An, Jeong-Wook;Park, Ok-Kyoung;Yoon, Hye-Ryoung;Yang, Young-Ho;Lee, Kyoung-Ryul
    • Journal of Genetic Medicine
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    • v.7 no.1
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    • pp.59-66
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    • 2010
  • Purpose: Quantitative fluorescent polymerase chain reaction (QF-PCR) allows for the rapid prenatal diagnosis of common aneuploidies. The main advantages of this assay are its low cost, speed, and automation, allowing for large-scale application. However, despite these advantages, it is not a routine method for prenatal aneuploidy screening in Korea. Our objective in the present study was to validate the performance of QF-PCR using short tandem repeat (STR) markers in a Korean population as a means for rapid prenatal diagnosis. Material and Methods: A QF-PCR assay using an Elucigene kit (Gen-Probe, Abingdon, UK), containing 20 STR markers located on chromosomes 13, 18, 21, X and Y, was performed on 847 amniotic fluid (AF) samples for prenatal aneuploidy screening referred for prenatal aneuploidy screening from 2007 to 2009. The results were then compared to those obtained using conventional cytogenetic analysis. To evaluate the informativity of STR markers, the heterozygosity index of each marker was determined in all the samples. Results: Three autosomes (13, 18, and 21) and X and Y chromosome aneuploidies were detected in 19 cases (2.2%, 19/847) after QF-PCR analysis of the 847 AF samples. Their results are identical to those of conventional cytogenetic analysis, with 100% positive predictive value. However, after cytogenetic analysis, 7 cases (0.8%, 7/847) were found to have 5 balanced and 2 unbalanced chromosomal abnormalities that were not detected by QF-PCR. The STR markers had a slightly low heterozygosity index (average: 0.76) compared to those reported in Caucasians (average: 0.80). Submicroscopic duplication of D13S634 marker, which might be a unique finding in Koreans, was detected in 1.4% (12/847) of the samples in the present study. Conclusion: A QF-PCR assay for prenatal aneuploidy screening was validated in our institution and proved to be efficient and reliable. However, we suggest that each laboratory must perform an independent validation test for each STR marker in order to develop interpretation guidelines of the results and must integrate QF-PCR into the routine cytogenetic laboratory workflow.

Analysis of Diagnostic Performance of CT and EUS for Clinical TN Staging of Gastric Cancer (위암의 임상적 병기 설정을 위한 전산화단층촬영 및 초음파 내시경의 진단력 평가)

  • Shin, Ru-Mi;Lee, Ju-Hee;Lee, Moon-Soo;Park, Do-Joong;Kim, Hyung-Ho;Yang, Han-Kwang;Lee, Kuhn-Uk
    • Journal of Gastric Cancer
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    • v.9 no.4
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    • pp.177-185
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    • 2009
  • Purpose: Preoperative clinical staging of gastric cancer is very important for determining the treatment plans and predicting the prognosis. The previous reports regarding the accuracy of computed tomography or endoscopic ultrasound for the preoperative staging of gastric cancer have shown various outcomes. We analyzed the diagnostic performance of CT and EUS, which are important staging tools for the staging of TN gastric cancer. Materials and Methods: We retrospectively analyzed 1,174 patients who underwent gastrectomy for gastric cancer at Seoul National University Bundang Hostpital from May, 2003 to December, 2007. We derived the Kappa value to examine the agreement of the preoperative staging obtained from CT and EUS with the pathological staging. Results: The mean age of the 1,174 patients was $59.31{\pm}11.98$ years. Six hundred thirty seven patients had early gastric cancer and 536 had advanced gastric cancer. The diagnostic performance between CT and EUS for the T staging showed no significant difference between CT and EUS for the kappa values. The kappa values showed moderate agreement at 0.4039 (P=0.021) and 0.4201 (P=0.026), respectively. This suggests that there is no difference between the two examinations for the overall T staging. Analysis of the discrimination of mucosal and submucosal lesions with EUS showed an accuracy of 58.92% and a Kappa value of 0.206 (P<0.001), suggesting fair agreement and a lower diagnostic performance than expected. To differentiate lesions with stages higher than or equal to T2 or T3 from the lesion with stages lower than T2 or T3, respectively, adoption of the higher stage from the CT staging or the EUS staging showed a larger AUC of 0.84 than that from either stage alone. The CT-derived node stage had the higher diagnostic performance (68.55%) than that of the EUS-derived node stage (60.82%) for the node staging. Conclusion: The CT-derived stage and EUS-derived stage showed comparable results for determining the T stage of gastric cancer. Yet the higher stage of the two stages from CT and EUS most accurately discriminated between those lesions with stages higher than T2 and those lesions with stages lower than T2.

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