• 한국해양생명과학회지
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• 제5권1호
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• pp.17-24
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• 2020

참다슬기 아가미 조직으로부터 heat shock protein 70 유전자를 분리·동정하였다. 참다슬기 HSP70 cDNA의 open reading frame (ORF)는 1,917 bp로 639개의 아미노산을 암호화하여 분자량은 약 70 kDa으로 예측되었다. 생물정보학 배열분석에 의해 HSP 유전자 기능과 관여되어 있는 3가지 주요 signature motifs와 보존된 도메인을 확인하였다. 계통학적 분석을 통하여 참다슬기 HSP70 유전자는 왕우렁이 Pomacea canaliculate와 같은 클러스트에 포함된다는 사실을 확인하였다. 수온 및 염분 변화에 따라, 참다슬기 HSP70 mRNA 유전자 레벨은 유의적으로 증가하였으며(p < 0.05), 이는 외부자극요인을 파악할 있는 분자생물학적 마커로서 활용될 수 있을 것으로 사료된다.

• Yonsei Medical Journal
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• 제59권9호
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• pp.1041-1048
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• 2018

Purpose: Heat shock factor 1 (HSF1) is a key regulator of the heat shock response and plays an important role in various cancers. However, the role of HSF1 in gastric cancer is still unknown. The present study evaluated the function of HSF1 and related mechanisms in gastric cancer. Materials and Methods: The expression levels of HSF1 in normal and gastric cancer tissues were compared using cDNA microarray data from the NCBI Gene Expression Omnibus (GEO) dataset. The proliferation of gastric cancer cells was analyzed using the WST assay. Transwell migration and invasion assays were used to evaluate the migration and invasion abilities of gastric cancer cells. Protein levels of HSF1 were analyzed using immunohistochemical staining of tissue microarrays from patients with gastric cancer. Results: HSF1 expression was significantly higher in gastric cancer tissue than in normal tissue. Knockdown of HSF1 reduced the proliferation, migration, and invasion of gastric cancer cells, while HSF1 overexpression promoted proliferation, migration, and invasion of gastric cancer cells. Furthermore, HSF1 promoted the proliferation of gastric cancer cells in vivo. In Kaplan-Meier analysis, high levels of HSF1 were associated with poor prognosis for patients with gastric cancer (p=0.028). Conclusion: HSF1 may be closely associated with the proliferation and motility of gastric cancer cells and poor prognosis of patients with gastric cancer. Accordingly, HSF1 could serve as a prognostic marker for gastric cancer.

• Journal of Animal Science and Technology
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• 제64권2호
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• pp.330-342
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• 2022

We aimed to investigate candidate proteins related to long-term caloric restriction and feed efficiency in bovine longissimus dorsi muscle (LM). A total of 31 Korean native steers were randomly distributed to ad libitum (n = 16) or caloric restriction group (n = 15) to conduct two feeding trials for 13 mon. In the first trial (10-18 mon of age), steers were fed with 100% ad libitum (NE_g = 0.63 Mcal/kg) or caloric restriction (80% of the previous day's feed intake of ad libitum group). In the second trial (18-23 mon of age), the energy value of 100% ad libitum diet was 1.13 Mcal/kg NE_g and those in caloric restriction group diet was 0.72 Mcal/kg NE_g. At the endpoint of this experiment, in each group, 6 animals were selected with high (n = 3) or low feed efficiency (n = 3) to collect muscle tissue samples (6 animals/group). From muscle tissues of 23 mo of age, we excavated 9 and 12 differentially expressed (two-fold or more) proteins in a nutritional group and feed efficiency group using two-dimensional electrophoresis, respectively. Of these proteins, heat shock protein beta-6 was up-regulated in both the caloric restriction and the low feed efficiency group. In bovine embryonic fibroblasts, the mRNA expression of heat shock protein beta-6 increased after adipogenic differentiation, however, decreased after myogenic differentiation. Our data provide that heat shock protein beta-6 may be an adipogenic protein involved in the mechanism of caloric restriction and feed efficiency in the LM of the steer.

• Advances in aircraft and spacecraft science
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• 제2권1호
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• pp.45-56
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• 2015

The attitude aerodynamic control is an important subject in the design of an aerospace plane. Usually, at high altitudes, this control is fulfilled by thrusters so that the implementation of an aerodynamic control of the vehicle has the advantage of reducing the amount of thrusters fuel to be loaded on board. In the present paper, the efficiency of a wing-flap has been evaluated considering a NACA 0010 airfoil with a trailing edge flap of length equal to 35% of the chord. Computational tests have been carried out in hypersonic, rarefied flow by a direct simulation Monte Carlo code at the altitudes of 65 and 85 km, in the range of angle of attack 0-40 deg. and with flap deflection equal to 0, 15 and 30 deg.. Effects of the flap deflection have been quantified by the variations of the aerodynamic force and of the longitudinal moment. The shock wave-boundary layer interaction and the shock wave-shock wave interaction have been also considered. A possible interaction of the leading edge shock wave and of the shock wave arising from the vertex of the convex corner, produced on the lower surface of the airfoil when the flap is deflected, generates a shock wave whose intensity is stronger than those of the two interacting shock waves. This produces a consistent increment of pressure and heat flux on the lower surface of the flap, where a thermal protection system is required.

• International Journal of Aeronautical and Space Sciences
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• 제10권1호
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• pp.1-14
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• 2009

When the stagnation temperature increases, the specific heat does not remain constant and start to vary with this temperature. The gas is perfect, it's state equation remains always valid, except, it was called by gas calorically imperfect or gas at high temperatures. The purpose of this work is to develop a mathematical model for a normal shock wave normal at high temperature when the stagnation temperature is taken into account, less than the dissociation of the molecules as a generalisation model of perfect for constant heat specific. A study on the error given by the perfect gas model compared to our model is presented in order to find a limit of application of the perfect gas model. The application is for air.

• Journal of Mechanical Science and Technology
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• 제14권3호
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• pp.298-305
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• 2000

The functionally graded material (FGM) is the new concept for a heat resisting material. FGM consists of ceramics on one side and metal on the other. A composition and microstructure of an intermediate layer change continuously from ceramics to metal at the micron level. This study is carried out to analyze the thermal shock characteristics of functionally graded PSZ/ metal composites. Heat-resistant property was evaluated by gas burner heating test using $C_2H_2/O_2$ combustion flame. The ceramic surface was heated with burner flame and the bottom surface cooled with water flow. Also, the composition profile and the thickness of the graded layer were varied to study the thermo mechanical response. Furthermore, this study carried out the thermal stress analysis to investigate the thermal characteristics by the finite element method. Acoustic emission (AE) monitoring was performed to detect the microfracture process in a thermal shock test.

• 생명과학회지
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• 제18권4호
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• pp.522-529
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• 2008

High-temperature requirement A2(HtrA2)는 대장균에서 42도 노출 시 세포 보호 기능을 하는 단백질인 HtrA의 human homologue로 동정되었다 현재까지 human HtrA2는 미토콘드리아에 존재하는 serine protaese로 세포사멸 기능에 관여하는 것으로 알려져 있으나, 그 생리적 기능 및 mammalian 세포 내에서 heat shock에 대한 보호기능에 대해서 명확히 알려진 바가 없다. 최근 HtrA2 유전자가 결실된 mouse embryonic fibroblast (MEF)가 보고되어 세포 내 HtrA2의 기능 연구가 가능해 졌으나, 이 세포에 대한 정보가 많은 부분 밝혀져 있지 않다. 생리기능연구를 위해서는 자체의 특성들에 대한 조사가 선행되어야 차후 기능연구가 가능할 것이다. 본 연구는 $HtrA2^{+/+}$, $HtrA2^{-/-}$ MEF 세포주를 확보하고, 두 세포주의 성장속도, 세포 형태 및, heat shock에 의한 세포사멸 정도를 측정하였다. 우선 $HtrA2^{+/+}$, $HtrA2^{-/-}$ MEF 세포주에서 HtrA2의 발현 유무를 PCR과 IB로 확인하였고, fractionation을 통해 $HtrA2^{+/+}$ 세포주에서만 HtrA2가 미토콘드리아에 위치함을 확인하였다. 두 세포에서 형태학적인 차이가 있음을 Coomassie staining으로 확인하였고, 성장속도 또한 $HtrA2^{-/-}$ 세포주가 1.4배 빠름을 확인하였다. 현재까지 보고되지 않은 HtrA2의 고온에 대한 반응연구를 위해 본 연구에서는 heat shock 자극에서 세포사멸을 측정하여, 기존에 알려진 세포사멸자극에서와 동일하게 heat shock에 의해서도 세포사멸이 야기됨을 확인하였다. $HtrA2^{+/+}$와 $HtrA2^{-/-}$ MEF 세포주를 이용한 연구에 있어, HtrA2 유무에 따른 세포의 생리학적 특징을 제공하였고, 향후 heat shock에 의한 세포사멸에서의 HtrA2 기능연구를 위한 중요한 기본 정보를 제공함으로써 HtrA2의 기능을 심도있게 연구하는데 사용할 수 있는 좋은 자료가 될 것이다.

• International Journal of Aeronautical and Space Sciences
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• 제18권3호
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• pp.425-435
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• 2017

In this work, an overview of flow diagnosis in a shock tunnel is made by means of using established techniques that are easy to setup, economical to arrange, and simple to measure. One flow condition was considered having Mach number of 6 at the nozzle-exit, regarded as freestream. Measured aerothermodynamic data such as shock wave speed, wall static and total pressures, surface heat flux, and shock stand-off distance ahead of test model showed good agreement with calculation. This study shows an overall procedure of flow diagnosis in a shock tunnel in a single manuscript. Outcomes are thought to be useful in the field of education and also in a preliminary stage of high-speed vehicle design and tests, that need to be performed within a short time with decent accuracy.

• 대한화학회지
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• 제42권4호
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• pp.398-410
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• 1998

Boltzmann 방정식의 비선형 해법으로서 cumulant 모멘트 방법을 연구하였으며, Maxwell 분자모형 단원자분자 기체계의 정상충격파 문제에 대하여 적용하였다. 모멘트 방정식의 해는 Maxwell-Ikenberry-Truesdell(MIT) 반복법을 사용하였다. 원래의 MIT 반복법은 초기값을 평형분포함수로부터 구하지만, 본 연구에서는 반복계산의 초기값을 Mott-Smith의 두방식(bimodal)함수로부터 구하였다. 모멘트 계산은 2차 반복단계까지 수행하였으며, 강한 충격파에 대한 밀도, 온도, stress, heat flux 등의 윤곽과 충격파의 두께, 그리고 마하수 1.4 미만의 약한 충격파의 두께를 계산하였다. 1차 반복계산에서 충격파 윤곽에 대한 간단한 형태의 해석적 표현을 얻었으며, 이로부터 도출한 약한 충격파 두께에 대한 극한법칙은 Navier-Stokes 이론과 정확히 일치한다. 2차 반복계산에 의한 결과는 강한 충격파의 윤곽곡선 및 충격파 두께가 Monte Carlo 문헌값과 정량적으로 일치함을 보인다.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.965-970
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• 2005

In our previous study [14], we found that heat-shock exposure did not stimulate the neutral trehalase activity in Sacchromyces cerevisiae KNU5377, but did in ATCC24858. Consequently, the trehalose content in KNU5377 became 2.6 times higher than that in ATCC24858. Because trehalose has been shown to stabilize the structure and function of some macromolecules, the present work was focused to elucidate the relationship between trehalose content of these strains and thermal stabilities of whole cells, through differential scanning calorimetry (DSC), and to predict critical targets calculated from the hyperthermic cell killing rates. These analyses showed that the prominent DSC transition of both strains gave identical $T_m$ (transition temperature) values in exponentially growing cells, and that the $T_m$ values of critical targets was about $3^{\circ}C$ higher in KNU5377 than in ATCC24858. Both heat-shocked KNU5377 and ATCC24858 cells displayed similar shifts in their DSC transition profiles. On the other hand, the $T_m$ value of the critical target of KNU5377 was decreased by $2.1^{\circ}C$, which was still higher than ATCC24858 showing no changes. In view of these results, the intrinsic thermotolerance of KNU5377 did not appear to result from the stability of entire cellular components, but rather possibly from that of particular macromolecules, including critical targets, even though it should be investigated in more details. Although the trehalose levels in heat-shocked cells are significantly different, as described in our previous study [14], the overall pattern of thermal stabilities and their predicted critical targets in two heat-shocked strains seemed to be identical. These data suggest that the trehalose levels examined before and after heat shock of exponentially growing cells are not closely correlated with the stabilities of whole cells and/or critical targets in both yeast strains.