• Title/Summary/Keyword: Heat exposure

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A Study on the Plasma Biochemical Indices of Heat-Stressed Broilers

  • Lin, H.;Du, R.;Gu, X.H.;Li, F.C.;Zhang, Z.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.9
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    • pp.1210-1218
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    • 2000
  • Four experiments were conducted to evaluate the effect of temperature and humidity on biochemical indices of Arbor Acres broilers at different weeks of age. The alkaline phosphatase (AKP), acid phosphatase (ACP), lactic dehydrogenase (LD), creatine kinase (CK), plasma glucose (Glu), calcium (Ca), potassium (K), chloride (Cl), urea nitrogen (UN), uric acid (UA), plasma thyroxin (T4), triiodothyronine (T3) and insulin levels were determined in all the four experiments. In experiment 1, the plasma Glu, LD and CK levels were increased by heat exposure ($35{^{\circ}C}$ and 35, 60, or 85% RH, 2 h) and this effect was aggravated by longer exposure (24 h). No significant changes (p>0.05) were found in Ca concentration, activity of AKP and ACP. In experiment 2, temperature (10, 20, 30, $33{^{\circ}C}$) had significant effect on the levels of K, Cl, UN, UA levels and the activity of LD (p<0.01), but had no significant influence on the activity of CK (p>0.05). The UN, UK and LD levels were elevated by low temperature $(10{^{\circ}C})$ (p<0.01), Cl content was increased by high temperature ($(33{^{\circ}C})$ (p<0.01), and K level was decreased by high ($(33{^{\circ}C})$ or low $(10{^{\circ}C})$ temperature and increased by medium temperature $(30{^{\circ}C})$ (p<0.01). The humidity (35, 85% RH) only had significant effect on Cl concentration which was decreased by high humidity (p<0.01). In experiment 3, the result showed that only the LD and CK activity were significantly increased (p<0.01) by high temperature (7, 24, 28, $32{^{\circ}C}$) or high humidity (35, 85% RH). Temperature and humidity had no significant effect on K, Cl, UA, UN and Glu levels (p>0.05). In experiment 4 (24, 27, 30, $33{^{\circ}C}$; 30, 45, 60, 75, 90% RH), plasma T3 level was declined by high temperature $(33{^{\circ}C})$, and this phenomena disappeared in birds under high temperature and high humidity environment. T4 concentration in plasma was not affected by temperature (p>0.05), but was increased by high or low humidity (p<0.01). Neither temperature nor humidity had significant effect on plasma insulin concentration (p>0.05). The results of the four experiments suggested that broilers at different growth periods might have different thermal requirements and would response differently to heat exposure. The plasma biochemical indices themselves had big variation; the reaction of the indices to thermal exposure treatment differed with the age of broilers. The big variation of biochemical indices themselves might cover the response of indices to temperature and humidity treatments.

Gene Expression of Chironomus riparius Heat Shock Protein 70 and Developmental Retardation Exposure to Salinity (염분 노출에 따른 Chironomus riparius의 성장지연과 Heat shock protein 70 유전자 발현)

  • Kim, Won-Seok;Im, Byeong-Hyeon;Hong, Cheol;Choi, Seung-Won;Park, Kiyun;Kwak, Ihn-Sil
    • Korean Journal of Ecology and Environment
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    • v.50 no.3
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    • pp.305-313
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    • 2017
  • We investigate that the impact of freshwater organism exposed to the salinity environment by the frequent rainfall following climate change. To evaluate the stress response following salinity exposure, we assessed the survival rate, molting success rate, the developmental period and mouthpart deformities in Chironomus riparius. In addition, we measured the molecular responses of biomarker gene, gene expression of heat shock protein 70 (HSP70) in C. riparius exposed to salinity after 96 hour. The C. riparius survival rates were showed on time dependent manner and not observed survival organisms above 15 psu at day 4. The pupation and emergence of C. riparius were not seen above 15 psu, and the molting success rate was less than 20% at 10 psu. The developmental retardation of C. riparius was well observed in the pupation and emergence period and was delayed by 4 days at 10 psu compared to the control and 5 psu. The mouthpart deformities after salinity exposure at 96 or 72 hour were observed at 10 psu and 15 psu. The expression of C. riparius HSP70 level was significantly increased exposure to 5 psu and 10 psu. Thus, salinity has been caused to be various ecotoxicological and molecular stress responses on freshwater organisms similar to harmful substances such as EDCs and so on.

Dietary Selection of Fat by Heat-stressed Broiler Chickens

  • Zulkifli, I.;Htin, Nwe Nwe;Alimon, A.R.;Loh, T.C.;Hair-Bejo, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.2
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    • pp.245-251
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    • 2007
  • A total of 160 d-old male broiler chicks (Cobb) were brooded for three weeks and then maintained at $24{\pm}1^{\circ}C$. Commencing from d 21, chicks were assigned to one of four feeding regimens: (1) diet with 8% palm oil (PO), (2) diet with 8% soybean oil (SO), (3) diet without added fat (control), (4) a choice of PO, SO and control (CH). The diets were formulated to maintain a constant ratio of energy and protein. From d 28 to 41, all birds were exposed to $34{\pm}1^{\circ}C$. The PO, SO and CH birds had greater body weight than controls on d 42. The PO but not SO diet reduced mortality rate, body temperature and serum creatine kinase level of broiler chickens during heat exposure. Although the total intake of control, PO and SO diets was not significantly different during heat exposure, the CH birds had lower creatine kinase activity and mortality rate than those provided SO diet but not significantly different from the birds fed control and PO diets. The relative abdominal fat weight and breast intramuscular fat content percentage were significantly lower in the control birds than those of PO, SO and CH groups. There were no significant differences in both parameters among the three latter groups. These findings suggest that the uncertainty of how much dietary fat to put into diets for heat stressed broilers can be overcome by allowing them to select their own consumption.

A heat shock cognate 70 gene in the endoparasitoid, Pteromalus puparum, and its expression in relation to thermal stress

  • Wang, Huan;Dong, Sheng-Zhang;Li, Kai;Hu, Cui;Ye, Gong-Yin
    • BMB Reports
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    • v.41 no.5
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    • pp.388-393
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    • 2008
  • The Pphsc70 (heat shock cognate 70) gene was isolated from the endoparasitoid Pteromalus puparum and then characterized. The full-length cDNA was 2204 base pair (bp) and contained a single 1968 bp ORF that encoded a polypeptide of 656 amino acids with a predicted molecular mass of 71.28 kDa. Phylogenetic analysis based on Hsc70 amino acid sequences from fifteen insect species agreed with the present phylogeny. In addition, genomic DNA confirmed the presence of three introns located at the coding region as well as the 5'UTR. A significant elevation of Pphsc70 expression was observed following heat treatment, however, continued exposure to heat shock or recovery caused the expression of induced mRNA to gradually decline to levels that were significantly lower than those of control pupae (P < 0.05). In addition, a significant increase was observed in the emergence rate of pupae that were preheated at $40^{\circ}C$ and then exposed to $50^{\circ}C$ for 1 h when compared with the pupae that were not preheated, but instead directly exposed to $50^{\circ}C$. Taken together, these results revealed that exposure to gradually increasing temperatures can enhance an insects thermo-tolerance.

Sag Behavior of STACIR/AW 410SQmm Overhead Conductor in accordance with the Aging (STACIR/AW 410SQmm 가공송전선의 경년열화와 이도거동(III))

  • Kim Shang-Shu;Kim Byung-Geol;Sin Goo-Yong;Lee Dong-Il;Min Byung-Uk
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.3
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    • pp.280-286
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    • 2006
  • As a way to expand electric capacity in conductor with electric power demand, STACIR/AW (Super Thermal-resistant Aluminum-alloy Conductors Aluminum-clad Invar-Reinforced) conductor which has high electric current and heat resistance characteristics have been developed. STACIR/AW power line is mechanical composite wire composed of steel cores for dip control and aluminum conductors for sending electric current. Recently, to ensure stable operation and prediction of wire life span of STACIR/AW conductor, a heat property of STACIR/AW conductor have been investigated. In the present work, a change of essential property with long term-heat exposure of STACIR/AW conductor and its structure material, INVAR wire and Al conductor, have been investigated. INVAR/AW is approximately $3.2\;{\mu}m/m^{\circ}C$. thermal expansion coefficient of INVAR/AW wire increases with time of heat exposure. the thermal expansion coefficient of INVAR/AW is markedly influenced by heat and mechanical treatment. creep rate(0.242) of STACIR/AW $410\;mm^2$ conductor at room temperature is much higher than that(0.022) at $210\;^{\circ}C$ STACIR/AW $410\;mm^2$ conductor has minimum creep rate at operating temperature. To lower creep rate with increase temperature is more unique characteristics in STACIR/AW. It is expected that STACIR/AW turned its tension to INVAR/AW at the transition temperature. at room temperature, the tension apportionment of INVAR/AW in STACIR/AW is about $50\;\%$. but whole tension of STACIR/AW is placed on the INVAR/AW alone of core metal above transition temperature.

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia

  • Amir Raoofi;Omid Gholami;Hossein Mokhtari;Fatemeh Bagheri;Auob Rustamzadeh;Davood Nasiry;Alireza Ghaemi
    • Clinical and Experimental Reproductive Medicine
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    • v.51 no.1
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    • pp.28-41
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    • 2024
  • Objective: Chronic scrotal hyperthermia (SHT) can lead to serious disorders of the male reproductive system, with oxidative stress playing a key role in the onset of these dysfunctions. Thus, we evaluated the impact of caffeine, a potent antioxidant, on cellular and tissue disorders in mice with chronic SHT. Methods: In this experimental study, 56 adult male NMRI mice were allocated into seven equal groups. Apart from the non-treated control group, all were exposed to heat stress. Two groups, termed "preventive" and "curative," were orally administered caffeine. The preventive mice began receiving caffeine immediately prior to heat exposure, while for the curative group, a caffeine regimen was initiated 15 consecutive days following cessation of heat exposure. Each treated group was subdivided based on pairing with a positive control (Pre/ curative [Cur]+PC) or a vehicle (Pre/Cur+vehicle). Upon conclusion of the study, we assessed sperm characteristics, testosterone levels, stereological parameters, apoptosis, antioxidant and oxidant levels, and molecular markers. Results: Sperm parameters, testosterone levels, stereological parameters, biochemical factors (excluding malondialdehyde [MDA]), and c-kit gene expression were significantly elevated in the preventive and curative groups, especially the former, relative to the other groups. Conversely, expression levels of the heat shock protein 72 (HSP72) and nuclear factor kappa beta (NF-κβ) genes, MDA levels, and apoptotic cell density were markedly lower in both caffeine-treated groups relative to the other groups, with more pronounced differences observed in the preventive group. Conclusion: Overall, caffeine attenuated cellular and molecular abnormalities induced by heat stress in the testis, particularly in the mice treated under the preventive condition.

Fabrication of Carbon Nanofiber/Cu Composite Powder by Electroless Plating and Microstructural Evolution during Thermal Exposure (무전해 도금에 의한 탄소나노섬유/Cu 복합 분말 제조 및 열적 안정성)

  • Kim In-soo;Lee Sang-Kwan
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2004.10a
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    • pp.39-42
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    • 2004
  • Carbon nanofiber/Cu composite powder has been fabricated by electroless plating process. Microstructural evolution of the composite powder after heat treatment under vacuum, hydrogen and air environment was investigated. A dispersed carbon nanofiber coated by copper was produced at the as-plated condition. Carbon nanofiber is coated uniformly and densely with the plate shaped copper particles. The copper plates on the carbon nanofiber aggregate during the thermal exposure at elevated temperature in vacuum and hydrogen in order to reduce surface energy. The thermal exposure of the composite powder in air at $400^{\circ}C$ for 3 hours leads to the spherodization of the composite powder owing to oxidation of copper.

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A Study on the Diffusion Behaviors in Weld Interface of Cr-Mo Steel/Austenitic Stainless Steel (Cr-Mo강/오스테나이트계 스테인리스강 용접재의 용접계면에서의 확산거동에 관한 연구)

  • 김동배;이상율;이종훈;이상용;양성철
    • Journal of Welding and Joining
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    • v.17 no.4
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    • pp.46-52
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    • 1999
  • Some of the pressurized reactor pressure vessels used in many chemical plants are made of low alloy carbon steel plates internally clad with an austenitic stainless steel for improved anti-corrosion properties. In this study, metallurgic structure of the weld interface of A 387 Grade12Class1 low alloy carbon steel claded with A182-F321 austenitic stainless steel after thermal exposure simulation heat treatment was investigated to display a characteristic behavior of dissimilar metal weld interface with thermal exposure during service at high temperature and pressure. EPMA, STEM, vickers-hardness test were performed and the results were correlated with the microstructure. To estimate the depth of the carburized/decarburized bands quantitatively, a model for carbon diffusion was proposed. The validity of the proposed theoretical relationships was confirmed by the directly measured data from the welded parts failed during service.

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Expression of Heat Shock Protein 70 Gene and Body Color Changes in Non-biting Midge Larvae (Glyptotendipes tokunagai) Effected by O3 Treatment (오존(O3) 노출에 의한 조각깔따구(Glyptotendipes tokunagai)의 체색 변화 및 heat shock protein 70 발현 변화)

  • Kim, Won-Seok;Choi, Bohyung;Kim, Moon-Kyung;Chae, Seon Ha;Kwak, Ihn-Sil
    • Korean Journal of Ecology and Environment
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    • v.53 no.4
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    • pp.324-330
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    • 2020
  • Ozone (O3) is a general disinfectant to remove micro-pollutants in water treatment system. Previous studies have reported effect of ozone to bacteria and pathogens removal, but its effect to the relatively large organisms has little known. In this study, we investigated potential effects of ozone toxicity to the non-bite midge larvae (Glyptotendipes tokunagai) with accumulate mortality, coloration change and expression of heat shock protein 70 (HSP70). The accumulate mortality rate of G. tokunagai increased in a dose-time dependent manner and the highest mortality rate was observed to 75% at 30 minute of exposure duration with 2.0 ppm of ozone concentration. Exposure to ozone was a factor increasing body color of the larvae. The tendency of HSP70 mRNA expression showed up-regulation in ozone exposure at 20 minute. After that time, the expression of HSP70 in exposed group decreased to a similar level of control group. Our results clearly showed that ozone toxicity affects physical and molecular activity of G. tokunagai, implying the potential hazardous of ozone in the aquatic ecosystem including macroinvertebrates.