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http://dx.doi.org/10.11614/KSL.2020.53.4.324

Expression of Heat Shock Protein 70 Gene and Body Color Changes in Non-biting Midge Larvae (Glyptotendipes tokunagai) Effected by O3 Treatment  

Kim, Won-Seok (Department of Ocean Integrated Science, Chonnam National University)
Choi, Bohyung (Fisheries Science Institute, Chonnam National University)
Kim, Moon-Kyung (Institute of Health & Environment, Seoul National University)
Chae, Seon Ha (K-water Research Institute, Korea Water Resources Corporation)
Kwak, Ihn-Sil (Department of Ocean Integrated Science, Chonnam National University)
Publication Information
Korean Journal of Ecology and Environment / v.53, no.4, 2020 , pp. 324-330 More about this Journal
Abstract
Ozone (O3) is a general disinfectant to remove micro-pollutants in water treatment system. Previous studies have reported effect of ozone to bacteria and pathogens removal, but its effect to the relatively large organisms has little known. In this study, we investigated potential effects of ozone toxicity to the non-bite midge larvae (Glyptotendipes tokunagai) with accumulate mortality, coloration change and expression of heat shock protein 70 (HSP70). The accumulate mortality rate of G. tokunagai increased in a dose-time dependent manner and the highest mortality rate was observed to 75% at 30 minute of exposure duration with 2.0 ppm of ozone concentration. Exposure to ozone was a factor increasing body color of the larvae. The tendency of HSP70 mRNA expression showed up-regulation in ozone exposure at 20 minute. After that time, the expression of HSP70 in exposed group decreased to a similar level of control group. Our results clearly showed that ozone toxicity affects physical and molecular activity of G. tokunagai, implying the potential hazardous of ozone in the aquatic ecosystem including macroinvertebrates.
Keywords
ozone; Glyptotendipes tokunagai; heat shock protein 70; body color; gene expression;
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