• Title/Summary/Keyword: Heat Stable

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Antibacterial Activity of an Ethyl Acetate Extract of Pseudomonas sp. UJ-6 against Methicillin-Resistant Staphylococcus aureus

  • Lee, Dae-Sung;Eom, Sung-Hwan;Je, Jae-Young;Kim, Young-Mog;Lee, Myung-Suk;Kim, Young-Man
    • Fisheries and Aquatic Sciences
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    • v.16 no.2
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    • pp.79-84
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    • 2013
  • In an effort to discover an alternative antibiotic for treating infections with methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas sp. UJ-6, a marine bacterium that exhibited antibacterial activity against MRSA, was isolated. The culture broth and its ethyl acetate extract exhibited bactericidal activity against MRSA. The extract also exhibited antibacterial activity against gram-negative bacteria, which were not susceptible to vancomycin. The treatment of MRSA with the extract resulted in abnormal cell lysis. The extract retained >95% of its anti-MRSA activity after heat treatment for 15 min at $121^{\circ}C$. Thus, although most antibiotics are unstable under conditions of thermal stress, Pseudomonas sp. UJ-6 produces a heat-stable anti-MRSA substance. The results of this study strongly suggest that Pseudomonas sp. UJ-6 can be used to develop a novel, heat-stable, broad-spectrum antibiotic for the treatment of MRSA infections.

A Study on Comparison of Capacity between Wet.Dry Floor Heating Systems Using Geothermal Heat Pump (지열히트펌프를 이용한 습식.건식 바닥난방 성능평가 연구)

  • Lee, Byoung-Doo;Lee, Se-Jin;Lee, Dae-Woo;Oh, Sung-Hae;Nam, Woo-Dong
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.11a
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    • pp.567-570
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    • 2009
  • The present study conducted comparative evaluation of wet dry floor heating systems using geothermal heat pump. Circulation hot water from geothermal heat pump which is $10{\sim}15^{\circ}C$ lower than that from boiler was used. In order to access indoor temperature ($25^{\circ}C$) it took 74 minutes for dry type and 247 minutes for wet type. Average floor temperature was $23.89^{\circ}C$ for wet type and $32.66^{\circ}C$ for dry type. Energy saving rate gradually increased by 66% after 138 minutes. In the results, for floor heating system using low temperature circulation water, wet type was not enough to meet stable and comfortable radiant floor heating due to low floor temperature and access time to indoor set temperature. While dry type was practicable for stable floor heating due to fast rise of indoor set temperature and comfortable floor temperature.

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Effect of Cerium on the Microstructure and Room Temperature Tensile Properties of Mg-4Al-2Sn-1Si Alloys (Mg-4Al-2Sn-1Si 합금의 조직 및 상온 인장 특성에 미치는 Ce의 영향)

  • Kim, Jung-Hoon;Cho, Dae-Hyun;Park, Ik-Min
    • Journal of Korea Foundry Society
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    • v.32 no.6
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    • pp.289-295
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    • 2012
  • Mg-Al-Sn-Si system alloy, as a promising cheap heat-resistant Mg alloy for automobile engine part, has been investigated. Refinement of microstructure and precipitation of thermally stable secondary phases are important goal for the design of heat-resistant Mg alloy. In this study, the effect of Ce on the microstructure and room temperature mechanical properties of Mg-Al-Sn-Si alloy was investigated. High thermally stable $Mg_2Si$ phases in Mg-Al-Sn-Si alloy is very useful intermetallic compound. However, the $Mg_2Si$ phases often result in poor mechanical properties due to the coarse chinese type $Mg_2Si$ phases. The experimental specimens were fabricated by fluxless melting under $CO_2+SF_6$ atmosphere and poured into the permanent pre-heated at $200^{\circ}C$. It was told that Ce addition can modify $Mg_2Si$ phases and refine microstructure and improve the tensile strength, yield strength and elongation.

Feasibility Study of High-Efficiency Ground Heat Exchanger using Double U-tube through a Real-Scale Experiment

  • Bae, Sangmu;Kim, Jaemin;Nam, Yujin
    • KIEAE Journal
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    • v.17 no.4
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    • pp.33-39
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    • 2017
  • Purpose: The use of renewable energy system is essential for building energy independence and saving energy consumption in the building sector. Among renewable energy technologies, ground source heat pump(GSHP) system is more energy-efficient and environmental-friendly than other heat source systems due to utilize stable ground heat source. However, the GSHP system requires a high initial installation cost and installation space in limited urban area, so it is difficult to have superiority in the market of heat source system. Therefore, it is necessary to develop the installation method of low-cost and improve system performance. This paper aims to evaluate the performance of double u-tube ground heat exchanger(GHX) and verify system feasibility through real-scale experiment. Method: In this study, the real-scale experiment of vertical closed-type GSHP system was conducted using double u-tube GHX and high-efficiency grout. Through the verification experiment, heat source temperature, heat exchange rate(HER) and seasonal performance factor(SPF) were measured according to the long-term operation. In addition, the feasibility analysis was conducted comparing to the single u-tube system. Result: In the results of experiment, average HER was 136.27 W/m and average SPF was 5.41. Furthermore, compared to the single u-tube, the installation cost of the developed system could be reduced about 70% in the same heating load condition.

An Experimental Study on the Heat Transfer Characteristics of a Grooved Heat Pipe for Solar Collector (그루브형 히트파이프를 갖는 이중진공관형 태양열 집열기의 열전달특성에 관한 실험적 연구)

  • Kim, Byoung-Gi;Chung, Kyung-Taek;Jang, Hwan-Young;Suh, Jeon-Se
    • Proceedings of the SAREK Conference
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    • 2006.06a
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    • pp.965-969
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    • 2006
  • This study investigated heat transfer characteristics between absorber and heat pipe used to extract heat from concentric evacuated tubular collectors. In order to experiment, T-type thermocouples are attached to a evaporator of heat pipe and absorber of inner tube. A wall temperature distribution of absorber and heat pipe were carried out by experimental method under actual various ir-radiance and outdoor conditions. As a result, As to increase an irradiance, a wall temperature of absorber and heat pipe is gradually increased. The heat pipe was required about 20min to obtain steady state operation after start up and operates stable during various irradiance conditions. And the collector efficiency was about $50{\sim}70%$ when a mass flow rate is about $1.3{\ell}/min$.

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A Study on the Heat Transfer Characteristics and Performance of the High Temperature Range Heater Plate Using Liquid-Vapor Phase Change Heat Transfer (기-액 상변화 열전달식 고온 히터 플레이트의 작동 특성과 성능에 관한 연구)

  • Kang, Hwan-Kook;Yim, Kwang-Bin
    • Journal of the Korean institute of surface engineering
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    • v.46 no.6
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    • pp.283-289
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    • 2013
  • The experimental study for the temperature uniformity on the wafer using liquid-vapor phase heat transfer mechanism is performed. For the experiment, the heater plate which is consist of stainless steel container, working fluid and electrical heater is designed, manufactured and tested at the range of 600 to $850^{\circ}C$. The results showed that the phase change type heater plate was much more uniform and stable temperature on the heater plate surface and wafer than the uniform heat flux type heater plate at the atmospheric condition. Also, the results of 300 mm outer diameter of heater plate showed that the same temperature uniformity compared with 230 mm.

Effects of natural convection on the melt/solid interface shape in the HEM process (열교환법 공정에서 고/액 계면의 형태에 미치는 자연대류의 영향)

  • 왕종회;김도현
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.1
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    • pp.41-46
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    • 1997
  • The change of flow field and the effects of convective heat transfer on the shape and location of melt/crystal interface has been studied during the crystal growth by the heat exchanger method. Although the thermal structure is stable in the crucible, the flow due to the natural convection driven by radial temperature gradient is significant, because the thermal stability is broken by the hemispherical melt/crystal interface shape. The maximum interface deflection with convection is smaller than without and the convective heat transfer should be considered to simulate the heat transfer process of heat exchanger method rigorously.

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Orthokinetic Stability of $\beta$-Lactoglubulin-Stabilized Emulsions : Effects of Protein Heat Treatment and Surfactant Addition

  • Hong, Soon-Taek
    • Preventive Nutrition and Food Science
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    • v.3 no.2
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    • pp.133-142
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    • 1998
  • Effects of protein heat treatment and surfactant additionoo the orthokindetic stability of $\beta$-lactoglobulin-stabilized emulsions have been investigated under turbulent flow conditions. In studies on protein-stabilized emulsions, samples which had been subjected to heat treatment(i.e. the protein solution orthe emulsion) have been found to be more prone to orthokinetic coalescene than the untreated ones. The emulsions stabilized with protein heated above the denaturation temperature(i.e. 7$0^{\circ}C$) showed the bigger initial average droplet size, which resulted in an increased orthokinetic coalescenece rate. The storage of the protein-stabilized emulsion at high temperature prior to the shearing experiment also made the emulsion less stable in the shear field. Interestingly. the addition of DATEM has been found to produce a substantial increase in orthokinetic stability of the heat-denatured protein-stabilized emulsion system, although Tween 20 is the opposite case.

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Oligomeric Characterization of GroESLx Chaperonin from Symbiotic X-Bacteric in Amoeba proteus

  • Jung, Gwang-Hyun;Ahn, Tae-In
    • Animal cells and systems
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    • v.6 no.3
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    • pp.227-232
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    • 2002
  • GroESLx proteins of symbiotic X-bacteria were overproduced in Escherichia coli and their structural characteristics were assayed after simple purification. The GroESx and GroELx were heat-stable at 8$0^{\circ}C$ and 5$0^{\circ}C$, respectively. After heat-treatment, GroESx was purified by DEAE Sephadex A-50 chromatography and GroELx was purified by step- and linear sucrose density gradient ultracentrifugation. Molecular masses of GroESx and GroELx were 50-80 kDa and 800 kDa, respectively, as estimated by sucrose density gradient ultracentrifugation. In chemical cross-linking analysis, subunits of GroESx were mostly cross-linked by incubation for 3 h in 0.4% glutaralde-hyde and GroESx was found to be composed of homo-heptamer subunits. Those of GroELx were cross-linked within 10 min in 0.3% glutaraldehyde and GroELx was in two stacks of homo-heptamer subunits. On the other hand, GroESx and GroELx proteins in a solution could not be cross-linked even after incubation for 3 h in 0.5% glutaraldehyde. GroELx was stable at 4-37$^{\circ}C$. In the presence of both GroESx and ATP, GroELx$_{14}$ was stable at 37$^{\circ}C$ but not at 4$^{\circ}C$ or 24$^{\circ}C$. Thus, we confirmed the oligomeric properties of GroESx$_{7}$ and GroELx$_{14}$ and their stability to heat and in the interaction with GroESx.x.

Turbulent Dispersion Behavior of a Jet issued into Thermally Stratified Cross Flows (II) (열적으로 성충화된 횡단류에 분류된 제트의 난류확산 거동 (II))

  • Kim, Sang Ki;Kim, Kyung Chun
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.23 no.11
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    • pp.1434-1443
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    • 1999
  • The turbulent fluctuations of temperature and two components of velocity have been measured with hot- and cold-wires in the Thermally Stratified Wind Tunnel(TSWT). Using the fin-tube heat exchanger type heaters and the neural network control algorithm, both stable ($dT/dz=109.4^{\circ}C$) and unstable ($dT/dz=-49.1^{\circ}C$) stratifications were realized. An ambient air jet was issued normally into the cross flow($U_{\infty}=1.0 m/s$) from a round nozzle(d = 6 mm) flushed at the bottom waII of the wind tunnel with the velocity ratio of $5.8(U_{jet}/U_{\infty})$. The characteristics of turbulent dispersion in the cross flow jet are found to change drastically depending on the thermal stratification. Especially, in the unstable condition, the vertical velocity fluctuation increases very rapidly at downstream of jet. The fluctuation velocity spectra and velocity-temperature cospectra along the jet centerline were obtained and compared. In the case of stable stratification, the heat flux cospectra changes Its sign from a certain point at the far field because of the restratification phenomenon. It is inferred that the main reason in the difference between the vertical heat fluxes is caused by the different length scales of the large eddy motions. The turbulent kinetic energy and scalar dissipation rates were estimated using partially non-isotropic and isotropic turbulent approximation. In the unstable case, the turbulent energy dissipation decreases more rapidly with the downstream distance than in the stable case.