• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.151-161
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• 2004

Transgenic plants have been studied as delivery system for edible vaccine against various diseases. Edible plant vaccines have several potential advantages as follows: an inexpensive source of antigen, easy administration, reduced need for medical personnel, economical to mass produce and easy transport, heat-stable vaccine without refrigerator, generation of systemic and mucosal immunity and safe antigen without fetal animal-virus contaminants. The amount of recombinant antigens in transgenic plants ranged from 0.002 to 0.8％ in total soluble protein, depending on promoters for the expression of interested genes and plants to be used for transformation. Throughout the last decade, edible plant vaccine made notable progresses that protect from challenges against virus or bacteria. However edible plant vaccines have still problems that could be solved. First, the strong promoter or inducible promoter or strategy of protein targeting could be solved to improve the low expression of antigens in transgenic plants. Second, the transformation technique of target plant should be developed to be able to eat uncooked. Third, marker-free vector could be constructed to be more safety. In this review we describe advances of edible plant vaccines, focusing on the yields depending on plants/promoters employed and the results of animal/clinical trials, and consider further research for the development of a new plant-derived vaccine.

• Journal of Korean Society on Water Environment
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• v.36 no.1
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• pp.55-68
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• 2020

Because of the intensified environmental problems such as climate change and resource depletion, sewage treatment technology focused on energy management has recently attracted attention. The conversion of primary sludge from the primary sedimentation tank and excessive sludge from the secondary sedimentation tank into biogas is the key to energy-positive sewage treatment. In particular, the primary sedimentation tanks recover enriched biodegradable organic matter and anaerobic digestion process produces methane from the organic wastes for energy production. Such technologies for minimizing oxygen demand are leading the innovation regarding sewage treatment plants. However, sewage treatment facilities in Korea lack core technology and operational know-how. Actually, the energy potential of sewage is higher than sewage treatment energy consumption in the sewage treatment, but current processes are not adequately efficient in energy recovery. To improve this, it is possible to apply chemically enhanced primary treatment (CEPT), high-rate activated sludge (HRAS), and anaerobic membrane bioreactor (AnMBR) to the primary sedimentation tank. To maximize the methane production of sewage treatment plants, organic wastes such as food waste and livestock manure can be digested. Additionally, mechanical pretreatment, thermal hydrolysis, and chemical pretreatment would enhance the methane conversion of organic waste. Power generation systems based on internal combustion engines are susceptible to heat source losses, requiring breakthrough energy conversion systems such as fuel cells. To realize the energy positive sewage treatment plant, primary organic matter recovery from sewage, biogas pretreatment, and co-digestion should be optimized in the energy management system based on the knowledge-based operation.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.1
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• pp.43-49
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• 2013

The use of ocean thermal energy conversion (OTEC) to generate electricity is one of the methods proposed to utilize renewable energy and to protect the environment. In this study, simulations were performed to investigate the effect of weather conditions in the Ulsan region, Korea, on the efficiency of a solar-heating OTEC (SH-OTEC) system. This system utilizes solar thermal energy as the secondary heat source. Various working fluids were also simulated to select one that is suitable for this system. The results showed that R152A, R600, and R600A, in that order, were the most suitable working fluids. The effective area of the solar collector for a $20^{\circ}C$ increase in the collector outlet temperature fluctuated from 50 to $97m^2$ owing to the change in the monthly average solar gain. The annual average efficiency of the SH-OTEC increases to 6.23%, compared to that of a typical conventional OTEC, which is 2-4%.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.5 no.2
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• pp.77-85
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• 2000

Simulation of local external forcing and its response in the rotation table experiment has been investigated. Spatially-uniform external forcings have been applied in many experimental studies, however, based on the fact that the north-south distribution of the wind-stress curl and the existence of local maximum of the sea surface heat loss in the northern part of the East Sea, new method of combined effects of local forcings has been employed in separate experiments. Carefully designed local source or sink at the bottom of the cylindrical container can produce horizontal pressure gradient within the Ekman layer, and consequently the interior also attains the same pressure gradient that produces geostrophic interior circulation. In order to keep free surface during the local-surface cooling, a side-wall cooling method is suggested. For the various type of local forcing including the effects local cooling and the periodic change of local wind-stress curl, western-boundary flow in terms of its strength, position of separation from the boundary have been observed.

• Korean Journal of Microbiology
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• v.44 no.2
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• pp.116-121
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• 2008

In my search of microbial source of novel enzymes, a marine actinomycetes, A1460, producing haloperoxidase was isolated from macroalgae from south sea, Korea and studied for physiological and biochemical properties. The haloperoxidation reaction was followed by the bromination of phenol red in the presence of hydrogen peroxide and potassium bromide. The haloperoxidase was partially purified from the cell extract with $35\sim75%$ ammonium sulfate precipitation, High-Q anion exchange chromatography, gel filtration chromatography, hydroxyapetite chromatography and hydrophobic interaction chromatography to a yield of 42% and purification fold of 70. This enzyme showed relatively high heat stability without losing 50% of activity after 1 hr incubation at $60^{\circ}C$. The highest activity was found at $45^{\circ}C$, and the optimal pH was about pH 7, but higher stability was observed at pH 8. Azide and cyanide ion showed strong inhibition at less than 1 $\mu M$ level suggesting that the enzyme was Fe ion dependent haloperoxidase.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.34 no.1
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• pp.56-64
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• 2006

This paper presents the development and application of a conducting shape memory polyurethane (CSMPU) actuator. While conventional shape memory polyurethanes were activated by external heat source, conducting shape memory polyurethanes introduced in 2004 are activated by electric power. Conducting shape memory polyurethane actuators were manufactured by adding carbon nano tube to conventional shape memory polyurethane. The main problem of the CSMPU developed in 2004 was bad dispersion of carbon nano tubes. In this paper, we tried to find how to solve the dispersion problem, and with a lot of elaborative works, conducting shape memory polyurethane actuators which had better electrical characteristics were developed. Then the actuation performance of the conducting shape memory polyurethane actuators was also measured and assessed. Finally, the possibility of applications were examined through the installation to Micro Air Vehicle.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.38 no.8
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• pp.747-757
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• 2010

The ice accreted on the airfoil is one of the critical drivers that causes the degradation of aerodynamic performance as well as aircraft accidents. Hence, an efficient numerical code to predict the accreted ice shape is crucial for the successful design of de-icing and anti-icing devices. To this end, a numerical code has been developed for the prediction of glaze ice accretion shape on 2D airfoil. Constant Source-Doublet method is used for the purpose of computational efficiency and heat transfer in the icing process is accounted for by Messinger model. The computational results are thoroughly compared against available experiments and other computation codes such as LEWICE and TRAJICE. The direction and thickness of ice horn are shown to yield similar results compared to the experiments and other codes. In addition, the effects of various parameters - temperature, free-stream velocity, liquid water contents, and droplet diameter - on the ice shape are systematically analyzed through parametric studies.

• Journal of Conservation Science
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• v.32 no.1
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• pp.11-19
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• 2016

Seungja Chongtong is a small korean tubular arm that made by particular arm-making way imitating a chinese arm. This study organizes scientific analysis results with results of inscriptions reading of Seungja Chongtong in the Central museum of Kyunghee University. Three of Seungja Chongtongs are made with Cu-Sn-Pb ternary alloy which is not much differ in contents and the results of microstructure analysis of Seungja Chongtong 1 which could investigate a microstructure among the Chongtongs were found out that Seungja Chongtong is made by general casting, with no evidences of an additional heat-treatment and tempering. Furthermore, in results of lead isotope ratio analysis to find out a source of lead using during the production of Seungja Chongtong, It seems that Seungja Chongtong is made with a lead ore from northern Gyeongsangbuk-do Province and southern Gangwon-do Province called Korea southern zone 2 area.

• Journal of Energy Engineering
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• v.15 no.2 s.46
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• pp.107-117
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• 2006

The status of water splitting thermochemical cycle for hydrogen production was reviewed in this article. Mass production of hydrogen could be possible using the thermochemical process which is similar to the concept of conventional chemical reaction system if the high temperature heat source is available. The mediators (chemicals and reagents) should be used to split chemically stable water, and should be recycled in a closed cycle in order to be environmentally acceptable. Though there is no process to reach commercial stage, IS cycle, two-step cycles based on metallic oxide such as ZnO/Zn, $Fe_3O_4/FeO$ and the associated cycles are attracted due to their possibilities of application. Development of materials for high temperature and/or corrosive conditions during thermochemical process is still important topic in some thermochemical processes.

• Mass Spectrometry Letters
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• v.5 no.1
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• pp.24-29
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• 2014

An advanced and reliable high performance liquid chromatography (HPLC)/ultraviolet detector (UV)/ion-trap time-of-flight (IT-TOF) mass spectrometry was developed for the simultaneous quantification of 19 marker compounds in Bang-poong-tong-sung-san (BPTS), a traditional oriental prescription. Various parameters affecting HPLC separation and IT-TOF detection were investigated, and optimized conditions were identified. The separation was achieved on a Capcell PAK C18 column ($1.5mm{\times}250mm$, $5{\mu}m$ particle size) using a gradient elution of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.1 mL/min. The column temperature was maintained at $40^{\circ}C$ and the injection volume was $2{\mu}L$. IT-TOF system was equipped with an electrospray ion source (ESI) operating in positive or negative ion mode. The optimized electrospray ionization parameters were as follows: ion spray voltage, +4.5 kV (positive ion mode), or -3.5 kV (negative ion mode); drying gas ($N_2$), 1.5 L/min; heat block temperature, $200^{\circ}C$. Automatic $MS^n$ (n = 1~3) analyses were carried out to obtain structural information of analytes. Elemental compositions and their mass errors were calculated based on their accurate masses obtained from a formula predictor software. The marker compounds in BPTS were identified by comparisons between $MS^n$ spectra from standards and those from extracts. Moreover, the libraries of $MS^2$ and $MS^3$ spectra and accurate masses of parent and fragment ions for marker compounds were constructed. The developed method was successfully applied to the BPTS extracts and identified 17 out of 19 marker compounds in the BPTS extracts.