• 한국동물위생학회지
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• 제32권4호
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• pp.369-375
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• 2009

This study was attempted to survey the prevalence of heartworm infection among 253 dogs (male 167, female 86) on the 16 breeding farms in southern Gyeongnam area in May to December 2007. The results obtained from this experiment were summarized as follows; Fifty seven (22.5%) of the 253 examined dogs were heartworms-antigen positive, while twenty five dogs were Microfilaria positive by the modified Knott's method. The regional infection rates were of 25% in Tongyeong and 21% in Goseong, and on the breeding environment aspects, infection rates were lower in rural area than urban area farms. The infection rates were higher in female (26/86, 30.2%) than male dog (31/167, 18.6%). The infection rates by breeding size of farms were 31% (11/35) at <20 head, 26%(35/135) at 20~50 head and 13% (11/82) at >50 head. The infection rates of heartworm in examined dogs at the age of <2, 2~4 and >5 were 10.7% (8/75), 26% (40/154) and 37.5% (9/24), respectively. In the microfillaria-infected 25 dogs, identified Dirofilaria immitis of 21 (84%) using PCR. The dogs infected with heartworm showed the increased number of eosinophil.

• 한국동물위생학회지
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• 제22권1호
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• pp.25-35
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• 1999

The prevalence of canine heartworm(Dirofilaria immitis) infection in 150 mixed-breed dogs(male : 54, female : 96) from February to December 1997 was investigated by using antigen test kit(ICT $GOLD^{TM}$ HW, Synbiotics, USA) based on immunochromatographic assay in Inchon city. Also, gross and histopathological findings of an antigen positive dog were carried out. The results were summarized as follows ; 1. Four dogs were positive from 150 tested dogs(2.7%). They were all more than 2 years old and infection rates in male and outdoor dogs was higher than those in female and indoor, respectively. Species of infected dogs were Pug(2) , German Sheperd(1) and Great-dane (1). 2. Regional infection rates were closely related with housing system in the city. 3. Pathological findings of antigen-positive dog was excessive enlargement, congestion and hemorrhage of lung and D immitis in heart and histologically hemosiderin, hypertrophy of pulmonary alveoli wall and irregular hypertrophy of pulmonary artery inner wall. Microfilaria was observed in pulmonary artery and arteriole, ventricle and splenic artery.

• 한국임상수의학회지
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• 제18권3호
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• pp.297-303
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• 2001

A female pug was presented with severe anemia, dyspnea and hemoglobinuria. By B- and M-mode echocardiography, the large number of heartworm were founded in the right ventricle, right atrium and pulmonary artery. The heartworm mass was moved from the right ventricle to the right atrium with the motion of the tricuspid value. Paradoxic septum motion were visible in M-mode of left ventrcular outflow tract and chordae tendineae level short-axis view. Flattened interventricular septum was find in B-mode of papillary muscle level short-axis view. In autopsy, 81 adult heartworms and 7 filarial debris were found. Most of adult heartworm were found in the right ventricle and pulmonary artery. 3 of them were found in the venaca and right atrium, respectively.

• 한국임상수의학회지
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• 제18권1호
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• pp.78-81
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• 2001

A 12-year old female Pointer was referred to the Veterinary Teaching Hospital, College of Veterinary Medicine, Kyungpook National University for evaluation of emaciation. The owner had noted a dysuria and dyschesia 2 months before admitting to the hospital. The patient showed anorexia, depression and mild anemia. Radiographic findings indicated the mass below the urinary bladder, which pushed the intestine up to the spine. The patient was necropsied and grossly, two large masses were found on the heart and cranial thorax. Congestive nodules of 0.5-1.5 cm in diameter were scattered on both sides of the lungs. The left ventricular wall was hypertrophied and adult heartworms were found in right atrium. A large mass, filled with blood, 9-10 cm in diameter, originated from the rectum, compressed urethra. The gall bladder was filled with bile juice and the spleen was mild atrophied. The cortex of the kidney and the intestine were congested. On microscopic observation, there were neoplastic cells forming irregular vascular channels in the lung, kidney and heart. The vascular channels contain variable amount of blood. In the lung, the cells were almost elongated and the nuclei of neoplastic cells were round or ovoid and hyperchromatized. Based on the observation, the case was diagnosed as a hemangiosarcoma.

• 한국임상수의학회지
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• 제30권1호
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• pp.36-40
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• 2013

Caval syndrome은 감염된 다수의 심장사상충이 우심방, 우심실을 포함한 우심계와 대정맥으로 이동하여 삼첨판의 움직임을 물리적으로 방해하고 폐동맥으로 가는 혈류의 장애를 유발하여 생명을 위협하는 질환이다. 이 질환의 치료를 위해 외과적인 사상충의 제거가 필요하다. 본 증례는 modified extraction brushes를 이용한 새로운 방법의 심장사상충 제거 방법에 대한 보고이다. 본 연구에서 새롭게 만들어진 사상충 제거 카테터를 사용하여 caval syndrome에 감염된 두 마리의 개를 성공적으로 치료하였다. 비록 새로운 시술법의 적용이 단지 두 증례에 불과하지만, 본 시술법은 심장 사상충 치료를 위한 외과적 제거방법 중 하나로 선택할 수 있다.

• 한국임상수의학회지
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• 제31권5호
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• pp.412-416
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• 2014

7년령 수컷 핏불테리어에서 두드러진 복부팽창과 심각한 운동 불내성으로 내원했다. 진단검사에서 우심의 확장과 확연한 폐동맥의 확장, 간질성 폐침윤과 우심방과 우심실에 많은 수의 사상충이 발견 되었다. 심초음파의 컬러와 연속 도플러에서 역시 심각한 폐성 고혈압을 동반한 삼첨판과 폐동맥의 역류가 나타났다. 104마리의 심장사상충이 루프 스네어(Snare, Boston Scientific, USA)를 이용한 심장중재술을 통해 제거되었다. 사상충의 제거 후 임상컨디션은 눈에 뛰게 회복되었다. 본 증례는 한국에서 첫 번째로 시행된 루프를 이용한 사상충 제거 케이스이다.

• Parasites, Hosts and Diseases
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• 제34권4호
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• pp.225-232
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• 1996

전국 5개 지역에서 사육되고 있는 German shepherd종 개 127두(암컷 56. 수컷 71두)를 대상으로 필라리아자충검사법(modified Knott's test)과 성충항원검사법($DiroCHEK^{\circledR}$, Synbiotics. USA)을 이용하여 1994년 10월부터 1995년 8월 사이에 개심장사상충 감염률을 조사하였다. 필라리아자충 검사법에 의한 감염률은 10.2%(13/127)였으나 항원검사법에 의해서는 28.3%(36/127)를 보여 항원 양성견 36두 중 말초혈액에서 필라리아자충이 검출된 경우는 33.3%에 불과하였다 필라리아자충이 검출된 개들은 모두 항원양성반응을 보였다. 항원양성반응 을 보였으나 필라리아자충이 검출되지 않은 은폐감염견들 중 3두를 부검한 결과 심장과 폐동맥에 4-15마리의 개심장사상충 성충이 존재하여 항원검사법이 보다 정확한 검사법임을 시사하였다. 항 원검사법에 의한 지역별 감염률에 있어서는 강원도 횡성지역의 개들이 가장 높았으며(84.4%). 예 천과 중원지역은 20.0%와 14.3%로 각각 조사되었고 김해와 광주지역의 개들에서는 검출되지 알았다. 감염견들의 연령은 1-3세, 4-6세, 그리고 7-11세에서 6.3%. 21.4% 및 56.4%로 각각 조 사되어 연령이 증가함에 따라 감염률도 증가하였다 이 연구의 결과 국내 개심장사상충의 감염률은 필라리아자충검사법에 의한 과거 조사(3.1~23.0%)에 비해 성충항원검사법에 의한 것이 보다 높을 것으로 추정 되었다.

• Parasites, Hosts and Diseases
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• 제52권1호
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• pp.21-26
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• 2014

Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.