• Title/Summary/Keyword: Health of Structure

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Analysis of Microbial Diversity in Makgeolli Fermentation Using PCR-DGGE (PCR-DGGE를 이용한 막걸리발효에서 미생물 다양성 분석)

  • Kwon, Seung-Jik;Ahn, Tae-Young;Sohn, Jae-Hak
    • Journal of Life Science
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    • v.22 no.2
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    • pp.232-238
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    • 2012
  • Kumjungsansung-Makgeolli$^{(R)}$ is a traditional Korean rice wine that is fermented from traditional nuruk and rice. In this study, we performed the PCR-denaturing gradient gel electrophoresis (DGGE) analysis targeting the 16S and 28S rRNA genes to characterize bacterial and fungal diversity during Makgeolli fermentation. The predominant bacteria in the PCR-DGGE profile during Makgeolli fermentation were Lactobacillus spp. (Lactobacillus curvatus, L. kisonensis, L. plantarum, L. sakei, and L. gasseri), Pediococcus spp. (P. acidilactici, P. parvulus, P. agglomerans, and P. pentosaceus), Pantoea spp. (P. agglomerans and P. ananatis), and Citrobacter freundii; these were identified on the base of analysis of 16S rRNA gene sequences. The dominant bacterium during Makgeolli fermentation was L. curvatus. The predominant fungi in PCR-DGGE profile during Makgeolli fermentation were Pichia kudriavzevii, Saccharomyces cerevisiae, Asidia idahoensis, Kluyveromyces marxianus, Saccharomycopsis fibuligera, and Torulaspora delbrueckii, and these were identified on the basis of analysis of 28S rRNA gene sequences. The dominant fungal species during Makgeolli fermentation changed from P. kudriavzevii at 0-2 days incubation to S. cerevisiae at 3-6 days incubation. This study suggests that PCR-DGGE analysis could be a suitable tool for the understanding of microbial diversity and structure during Makgeolli fermentation.

TRAIL and Effect of Irradiation on Apoptosis of Cancer Cells (TRAIL과 방사선 조사가 암세포의 사멸에 미치는 효과)

  • Lee, Jaeseob;Jang, Seongjoo
    • Journal of the Korean Society of Radiology
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    • v.10 no.6
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    • pp.387-393
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    • 2016
  • Tumor using the efficient concomitant radiotherapy and chemotherapy to remove, prior to surgery and, either reduce the size of the tumor after surgery, or was can be made smaller, Or excised tumor, in a way to be removed, most conventional surgical method is surgical excision surgery therapy. And methods reduce or tumor size, or smaller, chemotherapy can kill tumor is administered selectively anticancer agent which increases the radioactive susceptible to tumor cells, sensitive to susceptibility to radiation are those which make it possible to respond to, either TRAIL methods of various biological cytostatic can deform the protein, by deforming the structure of the protein help to cell death it is known. In this paper, the HCT-116 cells thought to be a cancer cell to analyze the interaction of TRAIL and radiation. Experimental results, single use of TRAIL and radiation, results were compared with the control group, it was found to have no significant effect on each cell proliferation and apoptosis. Conversely treated with TRAIL, when treated in parallel radiation, it was possible to know that the HCT-116 cells significantly apoptosis occurs, The proportion of G1 ratio G0 also was found to have increased. TRAIL conclusion is increased apoptosis radiation defensive cells can know that increased radiosensitivity, also possible to alter the cell cycle, reduce cell proliferation ability stepwise it was possible. TRAIL is increased apoptosis, decreased cell proliferative capacity, it is considered to be possible to use as a radiation sensitizer.

Validity and Reliability Evaluation of Pregnancy Related Stress Scale (임부 스트레스 측정도구의 신뢰도 및 타당도 평가)

  • Lee, Hyejung;Seo, Minjeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.6
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    • pp.503-512
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    • 2017
  • The perceived stress of pregnant women is a potential contributor to adverse birth outcomes. Although the importance of the psychosocial well-being of pregnant women has been emphasized, there are fewreliable and valid instruments to measure the stress level of pregnant women in Korea. This study evaluated the psychometric properties of Ahn's pregnancy related stress scale (PSS) that was originally developed in 1984. Two hundred pregnant women completed the survey questionnaire, which was comprised of the PSS, depression scale, and demographic information. Principal component analysis and confirmatory factor analysis was used to test the construct validity. The concurrent validity was evaluated using the correlation with depression scores. Based on exploratory factor analysis and a consideration of conceptual meaning, a five-factor structure was extracted, explaining 57.25% of the variance: physical discomfort, fetus, parenting, spouse relationship, and housework. The goodness-of-fit indices showed an acceptable fit overall with the full model and acceptable internal consistency (Cronbach's alpha =.89). The concurrent validity was confirmed by a comparing with the depression score (r=.48, p <.001). The shortened PSS, as a valid and reliable scale, is recommended to be used to assess pregnancy-related stress and to develop stress managing interventions for pregnant women in clinical settings.

Comparison of structure modeling with perceived service quality, reason of selection, satisfaction and loyalty of consumers by type of driving range (골프연습장 유형에 따른 서비스품질 및 선택이유와 고객만족, 충성도의 구조모형 비교)

  • Shin, Min-Chul;Park, Keun;Ka, Kyung-Hwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.6
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    • pp.3490-3498
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    • 2014
  • This study examined the relationship among service quality perceived by consumers according to the types of driving ranges(I: indoor and outdoor), market segmentation, the reason for choice of the driving range, customer satisfaction and loyalty and analyze their causal relationship. To reach this goal, this study conducted a questionnaire research for consumers of driving ranges by dividing it into the indoor and outdoor ranges. The quest-factor analysis(SPSSWIN Ver 13.0), the confirmatory factor analysis and the structural equation modeling method (AMOS 6.0) were used to analyze the data collected for this study. Based on the findings of this study, the following conclusions were drawn: The reason for the choice in both indoor driving and outdoor driving had no direct influence on the customer loyalty, but it had an indirect influence on the customer loyalty through customer satisfaction and service expectation. In addition, the service expectation had a direct influence on the customer loyalty in both indoor driving and outdoor driving. Therefore, the customer satisfaction in both indoor driving and outdoor driving serve as a significant mediating variable to reinforce the relationship between the service quality and customer loyalty.

A Study of Relationship among Leisure Supports, Flow and Wellness among Active Senior of Leisure Activity Participants (액티브 시니어 여가활동 참여자의 여가지지, 여가몰입 및 웰니스 간의 관계 연구)

  • Seol, Su-Hwang;Choi, Jong-Woon;Ahn, Byoung-Wook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.8
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    • pp.140-146
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    • 2020
  • The purpose of this study was to investigate the relationship among leisure supports, flow, and wellness of active senior leisure active participants. The subjects of the study were adults recruited from Seoul, Gyeonggi-do, and Jenla-do. For data processing, frequency, reliability, confirmatory factor, correlation, and structure equaling modeling analyses were conducted. The results were as follows. First, leisure supports of active seniors had an influence on leisure flow (p<.01). Second, leisure supports of active seniors had an influence on wellness (p<.01). Third, leisure flow of active seniors had an influence on wellness (p<.01). At this point in time as an aging society, active seniors are participating in leisure activities in a continuous pursuit of a healthy and happy old age by identifying the relationship between leisure support, leisure flow and wellness. This study suggests that our society should make an effort to participate in leisure and wellness activities.

Fabrication of Electrospun PAN/FA Nanocomposites and Their Adsorption Effects for Reducing Volatile Organic Compounds (전기방사에 의한 PAN/FA 나노 복합재의 제조 및 휘발성 유기 화합물에 대한 흡착효과)

  • Ge, Jun Cong;Wang, Zi Jian;Yoon, Sam Ki;Choi, Nag Jung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.6
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    • pp.702-708
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    • 2018
  • Volatile organic compounds (VOCs), as a significant air pollutant, is generated mainly from the burning of fossil fuels, building materials using painting, etc. The inhalation of a certain amount of VOCs can be deleterious to human health, e.g., headaches, nausea and vomiting. In addition, it can also cause memory loss and even increase the rate of leukemia. Therefore, as one of the methods for reducing VOCs in air, polyacrylonitrile/fly ash (PAN/FA) composite nanofibrous membranes were fabricated by electrospinning. To observe their VOCs adsorption capacity, the morphological structure of PAN/FA nanofibrous mats was investigated by field emission scanning electron microscopy (FE-SEM), and the VOCs (chloroform, benzene, toluene, and xylene) adsorption capacity of PAN/FA membranes were tested by gas chromatography/mass spectrometry (GC/MS). The results indicated that the PAN nanofiber containing 40 wt. % FA powder had the smallest fiber diameter of 283 nm; they also showed the highest VOCs adsorption capacity compared to other composite membranes.

Communication Data Format Design for LEO Satellite with Packet Utilization Standard (Packet Utilization 개념을 이용한 저궤도 위성의 데이터 통신 포맷 설계)

  • Lee, Na-Young;Lee, Jin-Ho;Suk, Byong-Suk
    • Journal of Satellite, Information and Communications
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    • v.3 no.2
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    • pp.13-17
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    • 2008
  • The conventional telemetry system of Korean low-earth orbiting satellites has certain limitations in accommodating various missions. As the payload becomes complex, it requires very complicated operational concepts in terms of telemetry. With the current design, the telemetry formats have to be rebuilt whenever new payloads or operation concepts are involved, and many constraints in operation shall be produced due to the lacks of its flexibility. As the capability and performance of a satellite have been improved, the communication structure of the satellite should be improved to gather more telemetry data. For the efficiency of data handling, it is necessary to change the grid based telemetry system in which the downlink interval and types for telemetry was limited. Comparing the fixed data map such as grid type, the packet based telemetry system can be operated as flexible and various types of packet can be designed such as the dump packet and the event packet. The sequence of the packets can be modified or newly defined to manage the massive satellite state of health data. In this paper, a new strategy for the telemetry development partially derived from PUS (Packet Utilization Standard) of European Space Agency, which provides enhanced features for the accommodation of payloads & operational requirements, is presented.

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Effective Range Evaluation of Wireless Monitoring System for Monopile (모노파일용 원거리 무선 모니터링 시스템의 유효거리 평가)

  • Park, Kiwon;Lee, Jong-Sub;Choi, Changho;Byun, Yong-Hoon
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.6
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    • pp.91-100
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    • 2012
  • Wireless monitoring system for the structural health evaluation has a limit to the reliability of measured response. The objective of this study is to evaluate an effective measurement range of the wireless monitoring system on the analyzed data. For the wireless monitoring system, Bluetooth and Wi-Fi are applied to datalogger-receiver and receiver-personal computer, respectively. For the model of the monopile structure response, a laboratory-scale monopile is manufactured with Mono Cast Nylon and a lateral loading is applied by hammer impacting. Strain gauges attached on the model monopile are connected with the datalogger. The distances of datalogger-receiver and receiver-personal computer are changed for the evaluation of the measurement range. Experimental results show that the receiving rates of the response remain almost constant within limited distance, while the receiving rates dramatically decrease out of effective range. In addition, the receiving rates affect on the measured natural frequencies of the model monopile. This study suggests that the effective range evaluation of the wireless monitoring system may be used for the determination of a monitoring distance to the monopile installed in the offshore wind farm.

Microstructure of Spider Booklung as Bio-indicator for Monitoring Environmental Asbestos Exposure (거미 서폐의 미세구조와 석면노출 환경의 모니터링을 위한 생물지표적 유용성 평가에 관한 연구)

  • Kim, Chang-Mo;Moon, Myung-Jin
    • Korean Journal of Environmental Biology
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    • v.32 no.1
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    • pp.49-57
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    • 2014
  • Orb-web spiders can be considered as an effective bio-indicative animal to monitor the ecological air pollution of certain habitat since they have limited shifting ability during their life spans. In this study we revealed the fine structural characteristics of booklung in the orb-web spider Nephila clavata, and its bio-indicative significance in the monitoring environmental asbestos exposure. The high resolution scanning electron microscopy results suggest that the cuticular spikes near the atrium could be act as an effective filtering structure for dust materials. Furthermore, inner cuticular spikes which protruded across the air sacs, have the functions not only sustaining appropriate respiratory volume of each air sac but also help to enhance respiratory capacity by maximizing the gross surface area for gas exchange. Interestingly, in spite of short exposure, the asbestose fibrils were effectively captured on the surface of the atrial cuticular spikes. Furthermore, histologic damages were observed due to penetration of asbestos fibrils through air sacs and fixed on the hemolymph space. In addition, although accumulated mass of hemocytes were observed near the fibrils, there were no asbestos bodies or coagulated hemocytes were found on the surface of the fibrils, This could be mainly due to the short exposure period towards asbestos. Briefly, these results indicate the spider's booklung could be valuable tool in the detection ecological air pollutants.

Development and Validation of Perceived Stigma of Delirium Scale (섬망 환자의 지각된 낙인 척도 개발 및 타당성 검증)

  • Kim, Seon-Young;Kim, Sung-Wan;Kim, Jae-Min;Shin, Il-Seon;Yoon, Jin-Sang
    • Korean Journal of Psychosomatic Medicine
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    • v.23 no.2
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    • pp.121-128
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    • 2015
  • Objectives : This study developed and validated the Perceived Stigma of Delirium Scale(PSDS), which is designed to measure perceived stigma associated with delirium in patients suffering from that disorder. Methods : Based on a literature review of scales assessing stigma, a preliminary scale comprising seven items was developed. After recovering from delirium, 128 patients completed the PSDS and the Distress Thermometer (DT). Factor analysis was used to examine construct validity, and internal consistency and test-retest reliability were examined to ensure reliability. Concurrent validity was assessed using the correlation between the total scores on the PSDS and the DT. Results : Factor analysis yielded a single-factor structure from the seven candidate items. One item was excluded due to low factor loading. The internal consistency was computed and Cronbach's ${\alpha}$ was 0.85 for the total score. The overall test-retest reliability was 0.71, with items ranging from 0.58 to 0.83. The total score on the PSDS was significantly correlated with the DT score. Conclusions : The PSDS may be a reliable, valid instrument for evaluating perceived stigma in patients who have recovered from delirium. Further study of the perceived stigma by delirium patients is required to assess the implications of the PSDS for clinical practice and research.