Journal of the Korean Society of Radiology (한국방사선학회논문지)

The Korean Society of Radiology (한국방사선학회)
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TRAIL and Effect of Irradiation on Apoptosis of Cancer Cells

TRAIL과 방사선 조사가 암세포의 사멸에 미치는 효과

  • Lee, Jaeseob (Department of Radiologoical Technology, Gwangyang Health College) ;
  • Jang, Seongjoo (Dept. of Radiological Technology, Dongshin University)
  • Received : 2016.09.22
  • Accepted : 2016.10.30
  • Published : 2016.10.31
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Abstract

Tumor using the efficient concomitant radiotherapy and chemotherapy to remove, prior to surgery and, either reduce the size of the tumor after surgery, or was can be made smaller, Or excised tumor, in a way to be removed, most conventional surgical method is surgical excision surgery therapy. And methods reduce or tumor size, or smaller, chemotherapy can kill tumor is administered selectively anticancer agent which increases the radioactive susceptible to tumor cells, sensitive to susceptibility to radiation are those which make it possible to respond to, either TRAIL methods of various biological cytostatic can deform the protein, by deforming the structure of the protein help to cell death it is known. In this paper, the HCT-116 cells thought to be a cancer cell to analyze the interaction of TRAIL and radiation. Experimental results, single use of TRAIL and radiation, results were compared with the control group, it was found to have no significant effect on each cell proliferation and apoptosis. Conversely treated with TRAIL, when treated in parallel radiation, it was possible to know that the HCT-116 cells significantly apoptosis occurs, The proportion of G1 ratio G0 also was found to have increased. TRAIL conclusion is increased apoptosis radiation defensive cells can know that increased radiosensitivity, also possible to alter the cell cycle, reduce cell proliferation ability stepwise it was possible. TRAIL is increased apoptosis, decreased cell proliferative capacity, it is considered to be possible to use as a radiation sensitizer.

종양을 효율적으로 적출, 제거하기 위해서는 부수적으로 방사선 치료 및 항암화학요법을 이용하여 수술 전이나, 수술 후 종양의 크기를 줄이거나 작게 할 수는 있었으나, 종양을 적출하거나 제거하는 방법으로 외과적 절제수술요법이 가장 재래적인 수술 방법이다. 종양의 크기를 줄이거나 작게 하는 방법과, 종양을 사멸시킬 수 있는 항암화학요법은 방사성 감수성을 증가시키는 항암약제를 종양세포에 선택적으로 투여하여 방사선에 대한 감수성에 민감하게 반응 할 수 있도록 한 것이며, 다양한 생물학적인 세포증식억제 방법 중 TRAIL은 단백질을 변형 시킬 수 있으며 단백질 구조를 변형시켜 세포의 사멸에 일조를 하는 것으로 알려져 있다. 본 논문에서는 HCT-116세포를 암세포로 간주하여 TRAIL과 방사선과의 상호관계를 분석하였다. 실험결과 TRAIL과 방사선의 단독사용은 대조군과 비교해 본 결과 각각 세포증식과 세포자멸사에 유의적인 영향을 끼치지 않은 것으로 나타났다. 반대로 TRAIL로 처리하고, 방사선 조사를 병행해서 처리한 경우 HCT-116세포가 유의적으로 세포자멸사가 발생되었음을 알 수 있었고, G1대비 G0의 비율도 증가한 것으로 나타났다. 결론적으로 TRAIL은 방사선 방어적인 세포의 세포자멸사를 증가시켜 방사선 감수성을 증가시켰음을 알 수 있었으며, 또한 세포주기를 변화시켜 세포 증식 능력을 점진적으로 감소시킬 수 있었다. TRAIL은 세포자멸사를 증가시키고 세포증식 능력을 감소시켜 방사선 증감제로서 사용이 가능하다는 것으로 사료된다.

Keywords

References

  1. Byung Geol Jeong, Soon Young Kim, Kon Joo Lee and Jong Sik Kim, "Up-Regulation of NAG-1 and p21 Genes by Sulforaphane," Journal of Life Science,Vol. 22, No. 3, pp. 360-365, 2012. https://doi.org/10.5352/JLS.2012.22.3.360
  2. Jihae Lee, Kyung-Ja Leeongyeon Lee, "Clinical Factors Predicting the Pathologic Tumor Response after Preoperative Concurrent Chemoradiotherapy for Rectal Cancer," Journal of radiation oncology, Vol. 26, No.4, pp. 213-221, 2008.
  3. O'Connell MJ, Martenson JA, Wieand HS, et al. "Improving adjuvant therapy for rectal cancer by combining protracted-infusion fluorouracil with radiation therapy after curative surgery" N Engl J Med, Vol. 331, pp. 502-507, 1994. https://doi.org/10.1056/NEJM199408253310803
  4. Gastrointestinal Tumor Study Group. "Prolongation of the disease-free interval in surgically treated rectal carcinoma," N Engl J Med, Vol. 312, pp.165-192, 1985. https://doi.org/10.1056/NEJM198501173120307
  5. Krook JE, Moertel CG, Gunderson LL, et al. "Effective surgical adjuvant therapy for high-risk rectal carcinoma," N Engl J Med, Vol. 324, pp. 709-715, 1991. https://doi.org/10.1056/NEJM199103143241101
  6. Gastrointestinal Tumor Study Group. "Radiation therapy and fluoro uracial with or without semustine for the treatment of patients with surgical adjuvant aden ocarcinoma of the rectum," J Clin Oncol, Vol. 10, pp.549-557, 1992. https://doi.org/10.1200/JCO.1992.10.4.549
  7. Wang, S and S. W. El-Deiry "TRAIL and apoptosis induction by TNF-family death receptors" oncogene Vol. 22, pp. 862-863, 2001.
  8. French, L.E and J.Tschopp, "The TRAIL to selective tumor death," Nat. Med, Vol. 5, pp. 146-147, 1999. https://doi.org/10.1038/5505
  9. Srivastava, R.K. "TRAIL/Apo-2L: mechanisms and clinical applications in cancer," Neoplasia Vol. 3, pp. 535-546, 2001. https://doi.org/10.1038/sj.neo.7900203
  10. Anderson, D.M, E.Maraskovsky, W.L.Billingsley, W. C. Dougall, M.E. Tometsko, E.R.Roux, M.C. Teepe, R.F. DuBose, D.Cosman, L.Galibert, "A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function," Nature, Vol. 390, 175-179, 1997. https://doi.org/10.1038/36593
  11. Griffith, T.S. and D.H. Lynch, "TRAIL: a molecule with multiple receptors and control mechanisms" Curr. Opin. Immunol, Vol. 10, pp. 559-563, 1998. https://doi.org/10.1016/S0952-7915(98)80224-0
  12. Zhang, X.D., A. Franco, K. Myers, C.Gray, T.Nguyen and P.Hersey, "Relation of TNF-related apoptosis in ducing ligand (TRAIL) receptor and FLICE inhibitory protein expression to TRAIL induced apoptosis of melanoma," Cancer Res, Vol. 59, pp. 264-295, 1999.
  13. Dongyeon Lee, Seongjin Ko, Sesik Kang, Changsoo Kim, Donghyun Kim, Junghoon Kim, “Dose Evaluation of Childhood Leukemia in Total Body Irradiation,” Journal of Korean Society of Radiology, Vol. 7, No. 4, pp. 259-264, 2013. https://doi.org/10.7742/jksr.2013.7.4.259
  14. Karson S Putt, Grace W Chen, Jennifer M Pearson, Joseph S Sandhorst, Martin S Hoagland, Jung-Taek Kwon, Soon-Kyung Hwang, Hua Jin, Mona I Church well, Myung-Haing Cho, Daniel R Doerge, William G Helferich & Paul J Hergenrother, "Small-molecule activation of procaspase-3 to caspase-3 as a personalized anticancer strategy," Journal of Nature Chemical Biology, Vol. 2, pp. 543-550, 2006. https://doi.org/10.1038/nchembio814
  15. David Wallach, Mark Boldin, Eugene Varfolomeev, Rudi Beyaert, Peter Vandenabeele, Walter Fiers, “Cell death induction by receptors of the TNF family: towards a molecular understanding,” Journal of FEBS letters, Vol. 410, No. 1, pp. 96-106, 1997. https://doi.org/10.1016/S0014-5793(97)00553-X