• Genomics & Informatics
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• v.14 no.4
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• pp.216-221
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• 2016

Osteoporotic fractures (OFs) are critical hard outcomes of osteoporosis and are characterized by decreased bone strength induced by low bone density and microarchitectural deterioration in bone tissue. Most OFs cause acute pain, hospitalization, immobilization, and slow recovery in patients and are associated with increased mortality. A variety of genetic studies have suggested associations of genetic variants with the risk of OF. Genome-wide association studies have reported various single-nucleotide polymorphisms and copy number variations (CNVs) in European and Asian populations. To identify CNV regions associated with OF risk, we conducted a genome-wide CNV study in a Korean population. We performed logistic regression analyses in 1,537 Korean subjects (299 OF cases and 1,238 healthy controls) and identified a total of 8 CNV regions significantly associated with OF (p < 0.05). Then, one CNV region located on chromosome 20q13.12 was selected for experimental validation. The selected CNV region was experimentally validated by quantitative polymerase chain reaction. The CNV region of chromosome 20q13.12 is positioned upstream of a family of long non-coding RNAs, LINC01260. Our findings could provide new information on the genetic factors associated with the risk of OF.

• Food Science and Industry
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• v.43 no.2
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• pp.105-114
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• 2010

• Health Policy and Management
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• v.29 no.4
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• pp.422-430
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• 2019

Background: This study aimed to demonstrate the association between the education level of parents and adolescent depression feeling. Methods: We used the chi-square test and the multiple logistic regression model to analyze the data of the "Korean Youth Health Behavior Survey in 2018" (n=43,220) conducted by the Korea Centers for Disease Control and Prevention. Results: As a result of the analysis, a group whose parents' education level is 'above the university graduation (including the graduation of the college)' had statistically significant experience of depression than a group whose parents' education level is 'high school graduation.' Conclusion: When preparing a policy on adolescent depression feeling, we need to consider the association between the education level of parents and adolescent depression feeling. Especially, a higher level of education of parents needs attention and management to foster positive mental health rather than excessive academic pressure, especially in the parent-child relationship of the same gender.

• Genomics & Informatics
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• v.9 no.3
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• pp.127-133
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• 2011

The Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) is one of the major genomic resources for human genetics and immunological studies. Use of LCLs is currently extended to pharmacogenetic studies to investigate variations in human gene expression as well as drug responses between individuals. We evaluated four common internal controls for gene expression analysis of selected hematopoietic transcriptional regulatory genes between B cells and LCLs. In this study, the expression pattern analyses showed that TBP (TATA box-binding protein) is a suitable internal control for normalization, whereas GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is not a good internal control for gene expression analyses of hematopoiesis-related genes between B cells and LCLs at different subculture passages. Using the TBP normalizer, we found significant gene expression changes in selected hematopoietic transcriptional regulatory genes (downregulation of RUNX1, RUNX3, CBFB, TLE1, and NOTCH2 ; upregulation of MSC and PLAGL2) between B cells and LCLs at different passage numbers. These results suggest that these hematopoietic transcriptional regulatory genes are potential cellular targets of EBV infection, contributing to EBV-mediated B-cell transformation and LCL immortalization.

• Journal of Veterinary Science
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• v.21 no.3
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• pp.41.1-41.12
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• 2020

Background: The poultry red mite, Dermanyssus gallinae, is a serious problem in the laying hen industry worldwide. Currently, the foremost control method for D. gallinae is the implementation of integrated pest management, the effective application of which necessitates a precise monitoring method. Objectives: The aim of the study was to propose an accurate monitoring method with a reliable protocol for caged-layer poultry farms, and to suggest an objective classification for assessing D. gallinae infestation on caged-layer poultry farms according to the number of mites collected using the developed monitoring method. Methods: We compared the numbers of mites collected from corrugated cardboard traps, regarding with length of sampling periods, sampling sites on cage, and sampling positions in farm buildings. The study also compared the mean numbers of mites collected by the developed method with the infestation levels using by the conventional monitoring methods in 37 caged-layer farm buildings. Results: The statistical validation provided the suitable monitoring method that the traps were installed for 2 days on feed boxes at 27 sampling points which included three vertical levels across nine equally divided zones of farms. Using this monitoring method, the D. gallinae infestation level can be assessed objectively on caged-layer poultry farms. Moreover, the method is more sensitive than the conventional method in detecting very small populations of mites. Conclusions: This method can be used to identify the initial stages of D. gallinae infestation in the caged-layer poultry farms, and therefore, will contribute to establishment of effective control strategies for this mite.

• Health Policy and Management
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• v.26 no.4
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• pp.271-278
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• 2016

Background: This study purposed to analyze the relationship between regional obesity rates and regional variables. Methods: Data was collected from the Korean Statistical Information Service (KOSIS) and Community Health Survey in 2012. The units of analysis were administrative districts such as city, county, and district. The dependent variable was the age-sex adjusted regional obesity rates. The independent variables were selected to represent four aspects of regions: health behaviour factor, psychological factor, socio-economic factor, and physical environment factor. Along with the traditional ordinary least square (OLS) regression analysis model, this study applied geographically weighted regression (GWR) analysis to calculate the regression coefficients for each region. Results: The OLS results showed that there were significant differences in regional obesity rates in high-risk drinking, walking, depression, and financial independence. The GWR results showed that the size of regression coefficients in independent variables was differed by regions. Conclusion: Our results can help in providing useful information for health policy makers. Regional characteristics should be considered when allocating health resources and developing health-related programs.

• Journal of Animal Science and Technology
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• v.66 no.5
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• pp.1021-1033
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• 2024

Exosomes have been extensively studied as disease biomarker in humans, given their role in transporting bioactive molecules. However, despite the great potential of exosomes as noninvasive diagnostic markers and therapeutic nanocarriers for bovine diseases, few studies have been conducted on bovine exosome. Thus, this study aimed to quantitatively and qualitatively compare three isolation methods to identify a suitable method for bovine serum. Exosomes were isolated using ultracentrifugation alone (UC), a combination of ultracentrifugation and size exclusion chromatography (US), or membrane affinity-based exoEasy kit (EE). Isolated particles were evaluated using a range of complementary techniques. Transmission electron microscopy showed that all three isolation methods resulted in particles with a cup-shaped morphology. The particle concentration measured by nanoparticle trafficking analyzer of US was lower compared to those of UC and EE method. As a result of immunoblotting, exosome markers including TSG101, CD81, and HSP70 were detected in US particles, while in UC and EE, only TSG101 expression was confirmed. Particles isolated from UC and EE showed a contamination with the blood protein albumin, whereas particles from US did not show albumin contamination. In addition, to evaluate the possibility of using exosomes as biomarkers, the profiles of the small RNA in the exosomes were compared using the bioanalyzer 2100. As a result, in the EE method, the band of small RNA (25-200 nt) was most prominent, and in the US methods, a distinct band was observed in the small RNA range. Collectively, the purity of exosomes without non-exosomal contamination was highest in the US method. However, for the detection of small RNA, the EE method was found to be the most suitable. Therefore, the results suggest that the optimal isolation method varies depending on the specific purpose of exosome isolation.

• The Korean Journal of Community Living Science
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• v.23 no.4
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• pp.441-446
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• 2012

The aim of this study was to evaluate the effect of application of the contents of Korean Information Center of Agricultura Safety and Health (KICASH) with 80 subjects(male: 60, female: 20) from different provinces nationwide in 2011. Subjects were classified according to their sex and familiarity of computer and then categorized into 3 groups with poor, medium and good by self-evaluation. The test for the effectiveness and satisfaction about KICASH was conducted with likert scale from very bad (1point) to very good (5point). Subjects generally tended to give 4 points to contents. And they gave support to KICASH in that they showed about 4 point with intention of application of KICASH for safety and health irrespective of familiarity of computer. However, as they received the information passively, it will be needed to develop more interesting and various contents which they could get more helpful information for their health and safety actively using two-way information communication system in future. Therefore the study can helps improve farmer's health and safety through developing advanced health and safety information system.

• The Korean Journal of Health Service Management
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• v.7 no.4
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• pp.179-190
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• 2013

The main purpose of this study was to investigate the effect of the factors which cause the patients to choose hospital from the view of Christian missionary hospital. The subjects were 194 inpatients and 202 outpatients of the K Medical Center in Busan. The research had been conducted from 20 June to 31 July in 2013. The major results demonstrated that the medical and social services image factors strongly affected to the satisfaction and selectivity of patients. Social role image of religious medical institution should be considered important and it seems beneficent in CRM(Customer Relationship Management) based on this results.

• The Korean Journal of Health Service Management
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• v.5 no.3
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• pp.89-100
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• 2011

The present study aims to determine the grade and factors of the effects that influence the stress related to job finding of the university students majoring public health administration. The data was collected for 4days from June 7, 2011. Among a total of 360 cases of the questionaries, only 324 cases were used. To data were analyzed by frequency, t-test, ANOVA, correlation and regression using PASW statistics 18.0. From the analyses, it was found that school environment and study-related stress were some of major causes for stress in job finding. There was statistically significant difference in the sub-areas of job finding-related stress by gender, age, type of school, school grade, whether or not the graduating class and satisfaction with the major selected. There was statistically significant difference among the sub-areas of job finding-related stress such as academic achievements, characters, and school and family environments and whether in graduating class of collegians and their satisfaction with the major they chose turned out to exercise statistically significant impact upon the stress of the students majoring public health administration.