• 한국잠사곤충학회지
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• 제20권2호
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• pp.6-9
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• 1978

잠 103$\times$잠 104 와 그 역교, 잠107$\times$잠108 및 그 역교를 공시하여 6개 업자의 잠종비중별 부화율 및 실용부화율 시험에서 얻은 결과는 1. 난비중을 세분하여 부화율 실용부화율을 보면 경비중에 비하여 중비중 및 중비중의 난이 부화율 및 실용부화율이 높았다. 2. 일모체는 중모체에 비하여 경비중 및 중비중난에서 부화률이 높았고 실용부화율은 경비중난에서만 높았다. 3. 중모체는 비중별 실용부화율 및 부화율의 차이가 컸으며 이에 비하여 일모체는 부화율에는 차이가 없으나 실용부화율에서 비중별 차이를 인정할 수 있었다. 4. 제조업자별 중비중난의 부화율 및 실용부화율의 차이는 없으나 경비중, 중비중난에서는 현저한 차이를 나타내었다.

• 한국잠사곤충학회지
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• 제10권
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• pp.59-62
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• 1969

암최청을 요약하면 대략 다음과 같다. 1. 춘잠기에는 암최청이 소잠율을 높인다. 따라서 일제소잠이 가능하며, 그 비율은 80-90％를 상회하지만 소잠이 일일간 연기된다. 2. 추잠기의 암최청은 효과는 다소 있으나 암환경에서 이미 의잠이 부화하므로 추잠기 이야포의 결과를 가져오니 하추잠기에는 암최청을 하지 않는 것이 생리적이라고 고찰한다.

• 한국잠사곤충학회지
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• 제10권
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• pp.57-58
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• 1969

Artificial silkworm eggs must do washing the acid completely after soaking HCl. Author experimented following deacidification times: 30 minutes, 1 hour, 2 hours, 6 hours soaking and 12 hours soaking eggs in the fresh water after artificial hatching, and incubated room temperature. There was no difference 1 hour's with control but more than 1 hour deacidification influenced badly in hatching ratio. Especially above than 6 hours deacidification was not only delaying 1 day in hatching date but also decreasing in practical hatching ratio.

• 한국가축번식학회지
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• 제20권4호
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• pp.443-451
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• 1997

These studies were conducted to determine the sex of preimplantation Hanwoo embryos produced in vitro using polymerase chain reaction(PCR). Y chromosome specific and bovine speicific DNA primers were synthesized and tested for embryo sexing. Bovine IVF embryos were produced in TCM 199 and CR1aa medium, and classified by developmental stages on Day 7 to 9. The effects of developmental rates to bovine IVF blastocysts on sex ratio were also investigated using PCR methods. The results obtained in this study were as follows; 1. Developmental rates to blastocyst from IVM/IVF embryos in TCM 199 and CR1aa medium for 9 days were 23.5 and 30.2%, respectively, and there was significant difference between the media(P<0.05). 2. Male to female ratio of early, mid, expanded and hatching balstocyst produced on Day 7 were 0.7:1, 1.4:1, 2.2:1, and 2.5:1, respectively, and male embryos was significantly higher proportion in expanding and hatching blastocysts(P<0.01). 3. On Day 8, male to female ratio of early, mid, expanded and hatching blastocysts were 0.6:1, 1:1, 2.5:1, and 2.7:1, respectively. Both expanded and hatching blastocysts obtained a significantly higher proportion of males(P<0.01). 4. The male : female ratio of early, mid, expanded and hatching blastocyst produced on Day 9 was 0.6:1, 0.8:1, 1:1, and 2.2:1, respectively. Hatching blastocysts had a significantly higher ratio of males(P<0.01). The developmental rate of IVM/IVF embryos to blastocyst for 9 day culture was higher in CR1aa than that in TCM 199 medium. For the sex ratio by developmental stages of IVF embryos, male ratio was higher in expanded blastocyst but female in early blastocysts.

• 한국잠사곤충학회지
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• 11호
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• pp.55-57
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• 1970

1. 교잡종의 냉장침산잠종 재냉장 적기는 침산 후 48∼72 시간 즉 2∼3일이다. 2. 원종의 냉장침산잠종 재냉장적기는 침산후 72시간 즉 3일이다. 3, 교잡종이나 원종을 막논하고 냉장침산잠종의 재냉장적기는 일언으르써 침산후 72시이라고 판단되며 입고방법은 중간온도 보호의 영향력이 있다고 판단된다.

• 한국수산과학회지
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• 제54권6호
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• pp.948-953
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• 2021

To find the optimal eggs to larvae transforming temperature of walleye pollock Theragra chalcogramma, we investigated their hatching pattern, mortality, and larval deformities. Results showed that the hatching quality decreased as the water temperature increased and dropped sharply above 13℃. The highest hatching rate was achieved at 7℃, whereas the lowest was at 15℃. Dead eggs were not observed at 7℃; however, dead egg ratios were significantly high at 4.5℃ and above 13℃ (P<0.05). Mortality of larvae after hatching was not observed at 4.5℃, but was significantly high above 13℃. The DNA content of the larvae did not show any significant difference at all water temperatures; however, RNA content was the highest at 7℃ and was significantly low above 11℃. In addition, RNA/DNA ratio was the highest at 7℃. Larval deformities after hatching were low at 7-11℃ and higher at higher or lower water temperature. Therefore, the hatching index and larval health index of Walleye pollock eggs in this study show that 7℃ to 11℃ seems to be the optimal transforming water temperature range.

• Clinical and Experimental Reproductive Medicine
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• 제41권2호
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• pp.68-74
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• 2014

Objective: In search of an ideal method of assisted hatching (AH), we compared the effects of conventional micropipette-AH and laser-AH on the blastocyst formation rate (BFR) and blastocyst cell numbers. Methods: Four-to five-week-old ICR female mice were paired with male mice after superovulation using Pregnant mare's serum gonadotropin (PMSG) and hCG. The two-cell embryos were flushed from the oviducts of female mice. The retrieved two-cell embryos underwent one of five AH procedures: single mechanical assisted hatching (sMAH); cross mechanical assisted hatching (cMAH); single laser assisted hatching (sLAH); quarter laser assisted hatching (qLAH); and quarter laser zona thinning assisted hatching (qLZT-AH). After 72 hours incubation, double immunofluorescence staining was performed. Results: Following a 72 hours incubation, a higher hatching BFR was observed in the control, sMAH, cMAH, and sLAH groups, compared to those in the qLAH and qLZT-AH groups (p<0.05). The hatched BFR was significantly higher in the qLAH and qLZT-AH groups than in the others (p<0.05 for each group). The inner cell mass (ICM) was higher in the control and sMAH group (p<0.05). The trophectoderm cell number was higher in the cMAH and qLAH groups (p<0.05). Conclusion: Our results showed that the hatched BFR was higher in groups exposed the the qLAH and qLZT-AH methods compared to groups exposed to other AH methods. In the qLAH group, although the total cell number was significantly higher than in controls, the ICM ratio was significantly lower in than controls.

• 한국잠사곤충학회지
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• 제14권2호
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• pp.113-123
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• 1972

춘추잠기를 통하여 잠기별로 성적이 좋은 항목과 여타항목 성적간의 관계를 보면 일정한 경향을 발견할 수가 없으므로 종합적인 고찰을 하기가 지극히 곤란하나 대략 다음과 같이 요약할 수 있겠다.(Table Omitted) (1) 월년잠종 냉장입고시의 배자 발육계제와 부화율 및 실용부화율간 에는 어떠한 경향을 발견할 수가 없다. 그러나 부화율이 좋지 못한 잠종은 부화율이 낮을 뿐만 아니라 상견수견량과 화용율도 낮다. 실용부화율이 좋지 못한 잠종은 부화율이 약간 낮은 경향이 있으나 상견수견량과 부화율은 평균치 이상이거나 평균치에 근사하다. 따라서 실용부화율은 누에의 건강과는 관계가 적다고 할 수 있겠다. (2) 오교율은 상견수견량과 화용율에 영향을 미치리라고 생각되며 오교율이 높아지면 원잠이나 원잠견의 혼입률이 높아지는 것이다. 그리고 오교율은 잠견제조에 속하는 문제이며 양잠의 양적 및 질적 생산에 영향을 미치는 요인이라고 하겠으나 여건에 따라서는 원종과 교잡종간에 현격한 차가 나타나지 않는 것이라고 생각된다. 또한 월년잠종과 불월년잠종간의 평균오교율차는 교잡원종의 순도에 기인한 것이라 생각된다. (3) 상견수견량이 적은 잠종은 화용율이 낮은 경향이 있으니 상견수견량과 화용율간에다 관계가 있다고 생각된다. (4) 견층비율이 낮은 잠종은 화용율이 낮아지는 경향이 있다고 하겠다. 따라서 견층비율은 잠품종적인 문제 이외에 잠작과도 유관하다고 생각된다.

• 한국발생생물학회지:발생과생식
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• 제17권3호
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• pp.247-255
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• 2013

The gonadsomatic index (GSI) of mottled skate was the highest in April, GSI and HSI showed a reverse phase for its reproductive cycle. The fish had one pair of egg capsules, having 1 to 7 fertilized eggs, and spawned all the year round. When surveying the reproductive characteristics of females over 63 cm in disc width, we found the spawning peak was between April to June, and the appearance ratio of egg capsules was the highest in May (32.1%). The eggs were hatched at $8^{\circ}C$, $13^{\circ}C$, $18^{\circ}C$, water temperature (12.8 to $24.2^{\circ}C$), and the best hatching temperature was $18^{\circ}C$. The number of fish hatched was 4 to 5 fish/egg capsules, and the hatching rate was 100%. The sex ratios of hatching larvae were 45.5% female and 54.5% male. Therefore this study will provide fundamental data and information for artificial reproduction of the mottled skate.

• 한국양식학회지
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• 제21권3호
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• pp.176-180
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• 2008

본 연구는 인위적으로 배란시킨 뱀장어 Anguilla japonica 배란란의 과숙특징을 생화학적으로 밝히고, 과숙을 방지하기 위한 하나의 방법을 제시하였다. 사용된 뱀장어 성숙란의 수정률과 부화율, 부화 후 3, 10일 후 생존율과의 관계는 Y=0.7954X+32.448($R^2=0.8176$, P=6.35E-7), Y=1.0241X-26.401($R^2=0.8121$, P=5.95E-27) 및 Y=0.9314X-24.814($R^2=0.7619$, P=7.33E-14)였다. DNA 함량은 부화율의 감소와 함께 20% 이하의 부화율을 보이는 배란란에서 유의적으로 가장 낮은 0.653 pg/ug protein을 보였다(P<0.05). 그리고 이들의 비는 부화율이 20% 이하의 난에서 1.058로 유의적으로 가장 높은 비를 보였다(P<0.05). 또한 부화율에 따른 total alkaline protease와 ACPase 활성은 모든 부화율 범위에서 유의적인 차이를 보이지 않았다(P>0.05). 단백질 함량은 부화율 20% 이하 배란란에서 유의적으로 가장 높은 186.16 ug/mg eggs의 함량을 보였다(P<0.05). 따라서 뱀장어의 과숙란은 지속적인 단백질의 공급으로 정상란의 과숙화를 촉진하여 부화율이 저하된다. 결국 세포는 과숙으로 인해서 죽었기 때문에, 간에서 생성된 난황단백질의 공급을 차단하거나 공급속도를 늦추어서 복강 내 배란 후 과숙을 방지해야 할 것으로 판단된다.