• 대한유전성대사질환학회지
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• 제13권1호
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• pp.48-53
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• 2013

Tyrosinemia I (fumarylacetoacetate hydrolase deficiency) is an autosomal recessive inborn error of tyrosine metabolism that produces liver failure in infancy or a more chronic course of liver disease with cirrhosis, often complicated by hepatocellular carcinoma in childhood or early adolescence. We studied a 37-year-old woman with tyrosinemia I whose severe liver disease in infancy and rickets during childhood were resolved with dietary therapy. From 14 years of age, she resumed unrestricted diet with the continued presence of the biochemical features of tyrosinemia, yet maintained normal liver function. In adult years, she accumulated only a small amount of succinylacetone. Despite this evolution to a mild biochemical and clinical phenotype, she eventually developed hepatocellular carcinoma. Her fumarylacetoacetate hydrolase genotype consists of a splice mutation, IVS6-1G>T, and a novel missense mutation, p.Q279R. Studies of resected liver revealed the absence of hydrolytic activity and immunological expression of fumarylacetoacetate hydrolase in tumour. In the non-tumoral areas, however, 53% of normal hydrolytic activity and immunologically present fumarylacetoacetate hydrolase were found. This case demonstrates the high risk of liver cancer in tyrosinemia I even in a seemingly favorable biological environment. In this study of tyrosinemia I, Case 2 with negative succinylacetone accumulation and the recovery of acute liver failure was compared with Case 1. Diet restriction and NTBC treatment are crucial to prevent hepatocellular carcinoma until liver transplant can take place and cure the condition. Further studies are needed to examine cases where liver cancer did not result despite clinical symptoms/signs of tyrosinemia type I.

• 환경생물
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• 제32권4호
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• pp.306-310
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• 2014

본 연구에서는 톳 양식에서 pH 및 염분농도의 조절에 의한, 환경친화적인 해적생물 구제 방법을 개발하고자 하였다. 이를 위하여 톳의 내성범위를 구하고, 친환경적인 해적생물 구제법을 확립하고자 하였다. 톳에는 생리적 영향을 미치지 않는 친환경적인 해적동물 구제법으로서, pH 4 이하 조건과 pH 10 이상의 조건, 염분농도 10 psu 이하의 조건 및 44 psu 조건에서 효과가 있는 것으로 나타났다.

• 한국건축시공학회지
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• 제13권1호
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• pp.75-83
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• 2013

The prevention of safety accidents can be achieved through Harvey's 3E's: Engineering, Enforcement, and Education. On construction sites, however, enforcement and engineering did not prove to be valid ways of reducing the number of safety accidents, as enforcement and engineering have failed to overcome and/or supplement the realistic limits regarding the relatively lower education levels of construction workers and the communication issues with foreign workers. On the other hand, safety education can enable the advancement of safety management by enhancing the educational contents and their methods of delivery. To improve the efficiency of safety education via educational contents, this research conducted a qualitative and quantitative analysis on the validity of the newly educational contents by overviewing possible accident types in different work phases. Of the accident types identified by the qualitative analysis, neither highly important nor insignificant accident types were found, yet all the suggested types are found to be required. In addition, this study did not find any significant differences in the occurrence probability of safety accidents related to the level of importance of each type of accident. The qualitative analysis in this research verified that the suggested accident types can be valid, educational material that can take into account future safety accidents.

• 척추신경추나의학회지
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• 제5권1호
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• pp.57-66
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• 2010

항통을 주소로 내원한 환자의 크론병에 대한 침구(鍼灸)치료로 복통 및 설사의 관해 상태가 되는 양호한 효과를 얻었기에 보고하는 바이다. 다만, 치료 기간이 짧아 증상의 변화의 추적이 필요한 상태이며, 다양한 임상적 연구를 위한 임상사례 데이터가 축적되어야 할 것으로 사료된다.

• 한국해양바이오학회지
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• 제8권1호
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• pp.30-38
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• 2016

The objective of this study was to investigate the useful components and potential antioxidant activities of 30 marine algae, collected from Jeju Island in Korea. The contents of bioacitve substance of extracts from marine algae, such total polyphenol and carbohydrate, were determined. The extract of Distromium decumbens had the highest amount of total polyphenol content (21.27%), and that of Gracilaria incurvata Okamura had the highest amount of total carbohydrate content (10.18%). The antioxidant activities of extracts obtained from algae were tested through the evaluation of DPPH radical and hydroxyl radical scavenging activity. The extracts of Distromium decumbens, Sargassum hemiphyllum (Turner) C.Agardh, Sargassum serratifolium (C. Agardh) C. Agardh and Acrosorium yendoi Yamada were found to have more than 80% DPPH radical scavenging activity and that of Dictyota okamurae (Dawson) $H{\ddot{o}}tning$, Schnetter, et Prud'homme van Reine, Myagropsis myagroides (Martens ex Turner) Fensholt, Sargassum serratifolium (C. Agardh) C. Agardh and Cladophora wrightiana Harvey showed more than 50% hydroxyl radical scavenging activity. These results suggest that algae collected from Jeju Island would be good raw materials for antioxidant.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.999-1010
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• 2016

Ganoderma lucidum BCCM 31549 has a long established role for its therapeutic activities. In this context, much interest has focused on the possible functions of the (1,3)-β-D-glucan (G) produced by these cultures in a stirred-tank bioreactor and extracted from their underutilized mycelium. In the existing study, we report on the systematic production of G, and its sulfated derivative (GS). The aim of this study was to investigate G and its GS from G. lucidum in terms of their antibacterial properties and cytotoxicity spectrum against human prostate cells (PN2TA) and human caucasian histiocytic lymphoma cells (U937). ¹H NMR for both G and GS compounds showed β-glycosidic linkages and structural similarities when compared with two standards (laminarin and fucoidan). The existence of characteristic absorptions at 1,170 and 867 cm^-1 in the FTIR (Fourier Transform Infrared Spectroscopy) for GS demonstrated the successful sulfation of G. Only GS exhibited antimicrobial activity against a varied range of test bacteria of relevance to foodstuffs and human health. Moreover, both G and GS did not show any cytotoxic effects on PN2TA cells, thus helping demonstrate the safety of these polymers. Moreover, GS showed 40% antiproliferation against cancerous U937 cells at the low concentration (60 μg/ ml) applied in this study compared with G (10%). Together, this demonstrates that sulfation clearly improved the solubility and therapeutic activities of G. The water-soluble GS demonstrates the potential multifunctional effects of these materials in foodstuffs.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2010년도 한국우주과학회보 제19권1호
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• pp.37.5-38
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• 2010

경희대학교 우주탐사학과에서는 우주공간 탐사를 위해 Trio(TRiplet Ionospheric Observatory)-CINEMA(Cubesat for Ions, Neutrals, Electrons and MAgnetic fields)로 명명된 초소형 위성을 개발하고 있다. 과학임무는 지구 저궤도에서 고에너지 입자를 관측하는 것이며, 이를 위해 고에너지 (2~300keV) 입자 검출기와 자기장 측정기가 탑재된다. 저에너지 입자 검출기 시스템인 STEIN(SupraThermal Electrons, Ions, Neutrals)은 $1\times4$ Array의 개선된 실리콘 검출기와 이온, 전자, 중성입자를 분리할 수 있는 정전장 편향기, 그리고 신호를 처리하는 전자회로로 구성되어있다. 설계된 전자회로는 매우 작은 검출기 기판, 아날로그 기판과 디지털 기판으로 이루어져 있고, 475mW 이하의 저 전력으로 동작한다. 또한 2~100keV의 에너지를 1keV이하의 해상도로 30,000event/sec/pixel 까지 관측 할 수 있도록 회로를 설계하였다. 센서로 들어온 입자로 인해 발생한 펄스의 신호는 4개의 아날로그 회로가 담당하게 되는데, Folded cascode amplifier를 배치하여 증폭률을 높인 Charge sensitive amplifier를 통해 신호를 증폭하고, $2{\mu}s$ unipolar gaussian shaping amplifier를 통해 읽기 쉽게 처리된 신호를 상한파고선별기와 하한파고 선별기를 통해 유효 값 여부를 판단하고, 피크 검출기를 통해 피크의 타이밍을 측정한 뒤 신호를 아날로그-디지털 변환 회로를 통하여 8bit의 값으로 나타내어, 입자들의 Spectrum을 측정하게 된다. 크기와 소비전력이 적음에도 검출성능이 우수하기 때문에 이 시스템은 향후 우주탐사 시스템에 있어 매우 중요한 역할을 수행 할 것으로 생각한다.

• Archives of Plastic Surgery
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• 제45권1호
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• pp.45-50
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• 2018

Background Free muscle flaps are a mainstay for reconstruction of distal third leg wounds and for large lower extremity wounds with exposed bone. However a major problem is the significant postoperative flap swelling, which may take months to resolve. We studied the efficacy and safety of immediate application of a vacuum assisted closure (VAC) dressing after a free muscle flap to the lower extremity. Methods Over a 19 months period, all consecutive free muscle flaps for lower extremity reconstruction at a Level I trauma center were evaluated prospectively for postoperative flap thickness, complications and flap survival. Immediate application of a VAC dressing was performed in 9 patients, while the flap was left exposed for monitoring in 8 patients. Results There was no statistically significant difference in flap survival between both cohorts. Mean flap thickness at postoperative day 5 for the VAC group was $6.4{\pm}6.4mm$, while flap thickness for the exposed flap group was $29.6{\pm}13.5mm$. Flap thickness was significantly decreased at postoperative day 5 for the VAC dressing group. Conclusions Immediate application of VAC dressing following free muscle flaps to the lower extremity does not compromise flap survival or outcomes and results in decreased flap thickness and a better aesthetic outcome.

• Archives of Plastic Surgery
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• 제49권6호
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• pp.773-781
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• 2022

Anatomies of the vascular and lymphatic systems have been vital research topics in reconstructive surgery. Harvey was a pioneer who provided the earliest descriptions of the cutaneous vasculature in the 17th century. The concept of vascular territories of the skin was first described by Manchot. The radiographic injection method in cadavers was developed by Salman, who defined more than 80 vascular territories. The arterial system has been thoroughly investigated with the development of regional and free flaps. The concept of axial and random pattern flaps was introduced by McGregor and Morgan. Manchot's vascular territories were refined by Taylor and Palmer as the angiosome concept. Detailed information about the venous circulation is essential for reconstructive surgeries. The concept of intrinsic and extrinsic venocutaneous vascular systems was introduced by Nakajima and led to the development of the venoadipofascial flap. The importance of venous augmentation in flap survival was emphasized by Chang. The lymphatic system was discovered much later than the arterial and venous systems. Aselli was credited for discovering the lacteal vessels in the 17th century; mercury was popularly used as a contrast agent to distinguish lymphatic vessels for the next three centuries. A radiographic method in cadavers was developed by Suami. Lymphatic imaging devices are constantly upgrading, and photoacoustic imaging was recently introduced for three-dimensional visualization of architecture of superficial layers of the lymphatic and venous systems.

• 한국어병학회지
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• 제37권1호
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• pp.133-138
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• 2024

This study is a report on combinational effects between four sesquiterpene lactones (SLs) from bay laurel (Laurus nobilis) leaves, and oxytetracycline (OTC) or amoxicillin (AMX) against four fish pathogenic bacteria such as Vibrio anguillarum, V. harveyi, Edwardsiella tarda, and Streptococcus iniae. Individually, four SLs exerted little antibacterial activity against fish pathogenic bacteria. However, when combined with OTC or AMX, they showed synergistic interaction against pathogenic bacteria. Especailly, zaluzanin C (1) reduced the MIC of OTC (or AMX) eight-fold. Our results showed that combinations of SLs with antibiotics (ABTs) are more effective than ABTs alone to control pathogenic bacteria. The highest synergistic effect was observed when zaluzanin C (1) was combined with OTC or AMX against V. harvey or S. iniae, displaying significant reductions of MICs up to 8-fold (0.125 to 0.015 ㎍/mL and 0.0078 to 0.0009 ㎍/mL). In addition, zaluzanin C (1) improved the antibiotic potency of OTC against OTC resistant V. harveyi (250 ㎍/mL to 62.5 ㎍/mL). Synergism between ABTs and phytochemical such as SLs could be a therapeutically helpful concept to improve the efficacy of ABTs and prevent antibiotic resistance. These results suggest that SLs can be used as an alternative to reduce antibiotic resistance in aquaculture.