• Korean Journal of Veterinary Service
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• v.32 no.1
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• pp.61-76
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• 2009

At the present study, it was aimed to explore the molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolates from pigs and cattle. A total of 138 Salmonella Typhimurium (S. Typhimurium) isolates were typed with phage, among them, 83.3% of S. Typhimurium tested could divide into a 10 phage types. Definitive type 193 (DT193) (25.4%) and DT195 (24.6%) were exhibited as the dominant types. DT104 and U302 were found from pigs and cattle. On the other hand, S. Enteritidis had 6 phage types, of them, phage type 21 (PT21) and PT11b were the popular types. In the plasmid profiles, 135 of S. Typhimurium isolates were exhibited 1 to 6 plasmid bands which molecular weight ranged from 90 to 2kb. 35 isolates (25.4%) harbored a 90kb plasmid which is thought to be the serotype specific virulence plasmid. Two of twenty five S. Enteritidis had common plasmids at 2 and 1.5kb. With multiplex polymerase chain reaction, virulence genes (invA and spvC) were detected from all Salmonella spp. from 167 of S. Typhimurium, S. Enteritidis and chloramphenicol resistant S. Schwarzengrund, but some drug resistant genes, such as PSE-1, cml/tetR and flo were not determined but other drug resistant genes, for example TEM and int were found. The detection rates of spvC, TEM and int gene was 35.3%, 29.3% and 72.5%, respectively. The TEM gene was highly popular in S. Typhimurium, which was detected from ampicillin and amoxicillin resistant strains as 95.9%. int gene was able to detect from all the isolates identified as multidrug resistsnt (MDR), particularly DT193 was thought as the most prevalent virulence and multidrug resistance isolate. The major plasmid profile and drug resistance pattern of DT193 were 90, 40, 10.5, 6.3, 3.0kb and ACCbDNaPSSuT, respectively. MDR was commonly found in other phage types, particularly DT104, U302 and DT203.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.9-16
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• 2012

This study was conducted to scientifically analyze on the trends of cultivation area and production, and to detect the suitable site for Morus alba cultivation in forest of Buan-gun for effective cultivation. During last 3 years, the cultivation area of M. alba has steadily been increased, and the mulberry production of Jeollabuk-do has been researched as the greatest in Korea. Especially Buan-gun has the largest cultivation area of M. alba. On the other hand, as the result of detecting the suitable site for M. alba cultivation by factors of altitude, aspect, slope, soil texture, and organism content using GIS, there was the 663 ha suitable site of the total of 16,608 ha. In this area, Byeonsan-myeon, Sangseo-myeon, and Jinseo-myeon were included with the largest area, therefore, it was considered that these were the favorable regions for the expansion of M. alba cultivation area. The result of this study would be expected to be used as the effective information when establishing the expansion plan for M. alba cultivation area focused on the forest of Buan-gun in the future.

• YAKHAK HOEJI
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• v.42 no.1
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• pp.25-30
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• 1998

This study was designed to compare the absorption fraction and extent of ketoprofen gels and a matrix typed ketoprofen plaster patch. 3g (90mg as ketoprofen) of the two gels whi ch has oleohydrogel or hydrogel as a base, respectively, and 3 pieces of plaster patches (90mg as ketoprofen) were, applied in the area of 210$cm^2$ on forearm in 12 volunteers by cross over design. Blood samples were collected serially up to 24 hours and the plasma concentrations of ketoprofen were analyzed by HPLC using flurbiprofen as an internal standard. The detection limit of the assay was 1ng/ml of ketoprofen in plasma. The pharmacokinetic parameters (e.g. $AUC_{24hr}$, $AUMC_{24hr}$, MRT, Fraction Absorbed) were calculated from the plasma concentrations time data of each volunteer. The oleo-hydrogel showed significantly higher absorption fraction and extent of ketoprofen than the current hydrogel. The mean plasma concentrations of the oleo-hydrogel were increased to 98.46${\pm}$23.15ng/ml by 6 hour after application, and increased futher to 100.61${\pm}$18.65ng/ml at 24 hour. On the other hand, those of the hydrogel were increased 17.61${\pm}$18.65ng/ml at 5 hour to 34.68${\pm}$9.65ng/ml at 24 hour gradually. Therefore the plasma concentrations of oleo-hydrogel at each measured time were 3~7 times greater than those of the hydrogel with statistical significance. The $AUC_{24hr}$ (1797.26${\pm}$52.09ng.h/ml) of the oleo-hydrogel was 3.5 times greater (P<0.05) than that (516.17${\pm}$104.52ng.h/ml) of the hydrogel. The plaster patches showed higher bioavailability ($AUC_{24hr}$ 2877.37${\pm}$578.27ng.h/ml) than the olea-hydrogel ($AUC_{24hr}$ 1797.26${\pm}$52.09ng.h/ml) without statistical significance. But the absorption fraction of the oleo-hydrogel was rather higher than that of the plaster patches during the first 6 hours after administration. These results suggest that newly developed ketoprofen gel which is used oleo-hydrogel as a base would show excellent skin permeation on topical application for the corresponding clinical indications and could be absorbed as well as plaster patches.

• Journal of the Korean Association of Geographic Information Studies
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• v.21 no.4
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• pp.26-49
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• 2018

The purpose of this study was to verify the reliability of the solar radiation model and discuss its applicability to the urban area of Seoul for summer heat stress mitigation. We extended the study area closer to the city scale and enhanced the spatial resolution sufficiently to determine pedestrian-level urban radiance. The domain was a $4km^2$ residential area with high-rise building sites. Radiance modelling (SOLWEIG) was performed with LiDAR (Light Detection and Ranging)-based detailed geomorphological land cover shape. The radiance model was evaluated using surface energy balance (SEB) observations. The model showed the highest accuracy on a clear day in summer. When the mean radiation temperature (MRT) was simulated, the highest value was for a low-rise building area and road surface with a low shadow effect. On the other hand, for high-rise buildings and vegetated areas, the effect of shadows was large and showed a relatively low value of mean radiation temperature. The method proposed in this study exhibits high reliability for the management of heat stress in urban areas at pedestrian height. It is applicable for many urban micro-climate management functions related to natural and artificial urban settings; for example, when a new urban infrastructure is planned.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.384-394
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• 2021

The aim of this study was to investigate the correlation between soil properties and marker compounds contents of Paeonia lactiflora. The methods of determining marker compounds were validated by measuring the linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy and recovery using UPLC analysis. P. lactiflora contained albiflorin at 0.04 ± 0.00 ~ 2.79 ± 0.21%, paeoniflorin at 1.98 ± 0.14 ~ 6.67 ± 0.84%. The root dry weight (RDW) of P. lactiflora was 0.06 ± 0.02 ~ 1.27 ± 0.28 kg. The soil properties analysis such as soil pH, electric conductivity (EC), organic matter (OM), total nitrogen (TN), available phosphate (Avail. P₂O₅), exchangeable cation and cation exchange capacity (CEC) were performed following standard analysis manual. The results of correlation analysis between soil properties and growth characteristics, available P₂O₅ was positively coreelated with the RDW of P. lactiflora. On the other hand, the RDW of P. lactiflora showed significantly negative correlation with contents of albiflorin and paeoniflorin. The results of this study was might be help to provide useful information on the establish of standard cultivation by the investigate correlation analysis between growth characteristics and marker compound contents of P. lactiflora.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.5
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• pp.81-88
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• 2021

Structural health monitoring (SHM) systems have attracted considerable interest owing to the frequent earthquakes over the last decade. Smart concrete is a technology that can analyze the state of structures based on their electro-mechanical behavior. On the other hand, most research on the self-sensing response of smart concrete generally investigated the electro-mechanical behavior of smart concrete under a static loading rate, even though the loading rate under an earthquake would be much faster than the static rate. Thus, this study evaluated the electro-mechanical behavior of smart ultra-high-performance concrete (S-UHPC) at three different loading rates (1, 4, and 8 mm/min) using a Universal Testing Machine (UTM). The stress-sensitive coefficient (SC) at the maximum compressive strength of S-UHPC was -0.140 %/MPa based on a loading rate of 1 mm/min but decreased by 42.8% and 72.7% as the loading rate was increased to 4 and 8 mm/min, respectively. Although the sensing capability of S-UHPC decreased with increased load speed due to the reduced deformation of conductive materials and increased microcrack, it was available for SHM systems for earthquake detection in structures.

• Journal of fish pathology
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• v.35 no.1
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• pp.93-102
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• 2022

This study performed a trichlorfon (TCF) residue and pharmacokinetic analysis with Japanese eels, Anguilla japonica, to obtain baseline data to establish the maximum residue level (MRL) of TCF in A. japonica. After dipping A. japonica in 30 ppm and 150 ppm of TCF at 28℃ and 18℃, drug residue in the body was analyzed with LC-MS/MS, and these results were further analyzed with the PK solver program to obtain the pharmacokinetic parameters of TCF in the serum, muscles, and liver. The maximum concentrations (C_max) in the serum, muscles, and liver were 25.87-357.42, 129.91-1043.73, and 40.47-375.20, respectively, and the time to maximum concentration (T_max) was 0.13-1.32h, 1.17-3.34h, and 0.14-5.40h, respectively. The terminal elimination half-life (T_1/2) was 2.13-3.92h, 5.30-10.35h, and 0.65-13.81h, respectively. In the 30 mg/L concentration group, TCF was not detected in the serum of eels 96 hours after bathing, and was below the detection limit after 336 hours in muscle and liver. On the other hand, in the 150 mg/L concentration group, TCF was not detected in the serum of eels 336 hours after bathing, but was detected in muscle and liver at 336 hours. In conclusion, the results of this study would be useful in establishing the MRL of TCF in farmed A. japonica.

• Journal of the Korean Geotechnical Society
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• v.23 no.4
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• pp.15-24
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• 2007

Two kinds of temperature monitoring technology have been introduced in this study, which can measure coincidently temperatures at many points along a single length of cable. One is to use a thermal sensor cable comprizing of addressable thermal sensors. The other is to use an optic fiber sensor with Distributed Temperature Sensing (DTS) system. The differences between two technologies can be summarized as follows: A thermal sensor cable has a concept of "point sensing" that can measure temperature only at a predefined position. The accuracy and resolution of temperature measurement are up to the capability of the individual thermal sensor. On the other hand, an optic fiber sensor has a concept of "distributed sensing" because temperature is measured practically at all points along the fiber optic cable by analysing the intensity of Raman back-scattering when a laser pulse travels along the fiber. Thus, the temperature resolution depends on the measuring distance, measuring time and spatial resolution. The purpose of this study is to investigate the applicability of two different temperature monitoring techniques in technical and economical sense. To this end, diverse experiments with two techniques were performed and two techniques are applied under the same condition. Considering the results, the thermal sensor cable will be well applicable to the assessment of groundwater flow, geothermal distribution and grouting efficiency within about loom distance, and the optic fiber sensor will be suitable for long distance such as pipe line inspection, tunnel fire detection and power line monitoring etc.

• Journal of Naturopathy
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• v.7 no.1
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• pp.20-25
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• 2018

The purpose of this study was investigated to observe the relatively low amino acid mutations in six different strains of ATCC or KCTC Salmonella strains (four species and two isolates). The mutations in the strains were to be detected and compared with the genes csgA and csgB corresponding to the Thin aggregative fimbriae. Amino acid mutations in the strains were observed from Ser20→ Gly(AGT→GGT) in the csgA gene, Asp25→Ala(GAT→GCT) and Lys66→Thr(AAA→ACA) in the csgB gene. Among the six strains, the two most common amino acid variations were observed in S. typhimurium - TH strains. On the other hand, no mutation of nucleotide sequence was observed in the strains of S. typhimurium ATCC 13311 and S. typhimurium KCTC 1925. In conclusion the genes csgA and csgB in the strains may be useful for the evaluation and detection of amino acid mutation.

• Korean Journal of Remote Sensing
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• v.39 no.6_1
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• pp.1225-1234
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• 2023

The intensity of crop water stress caused by moisture deficit is affected by growth and heat conditions. For more accurate detection of crop water stress state using remote sensing techniques, it is necessary to select vegetation indices sensitive to crop response and to understand their changes considering not only soil moisture deficit but also heat conditions. In this study, we measured the MERIS terrestrial chlorophyll index (MTCI) and chlorophyll/carotenoid index (CCI) under drought and heat wave conditions. The MTCI, sensitive to chlorophyll concentration, sensitively decreased on non-irrigation conditions and the degree was larger with heat waves. On the other hand, the CCI, correlated with photosynthesis efficiency, showed less sensitivity to water deficit but had decreased significantly with heat waves. After re-irrigation, the MTCI was increased than before damage and CCI became more sensitive to heat stress. These results are expected to contribute to evaluating the intensity of crop water stress through remote sensing techniques.