Browse > Article
http://dx.doi.org/10.7847/jfp.2022.35.1.093

Residue level and pharmacokinetics of trichlorfon in the Japanese eel (Anguilla japonica) after bath treatment  

Jo, Hyun Ho (Department of Aquatic Life Medicine, Pukyong National University)
Chung, Joon Ki (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.35, no.1, 2022 , pp. 93-102 More about this Journal
Abstract
This study performed a trichlorfon (TCF) residue and pharmacokinetic analysis with Japanese eels, Anguilla japonica, to obtain baseline data to establish the maximum residue level (MRL) of TCF in A. japonica. After dipping A. japonica in 30 ppm and 150 ppm of TCF at 28℃ and 18℃, drug residue in the body was analyzed with LC-MS/MS, and these results were further analyzed with the PK solver program to obtain the pharmacokinetic parameters of TCF in the serum, muscles, and liver. The maximum concentrations (Cmax) in the serum, muscles, and liver were 25.87-357.42, 129.91-1043.73, and 40.47-375.20, respectively, and the time to maximum concentration (Tmax) was 0.13-1.32h, 1.17-3.34h, and 0.14-5.40h, respectively. The terminal elimination half-life (T1/2) was 2.13-3.92h, 5.30-10.35h, and 0.65-13.81h, respectively. In the 30 mg/L concentration group, TCF was not detected in the serum of eels 96 hours after bathing, and was below the detection limit after 336 hours in muscle and liver. On the other hand, in the 150 mg/L concentration group, TCF was not detected in the serum of eels 336 hours after bathing, but was detected in muscle and liver at 336 hours. In conclusion, the results of this study would be useful in establishing the MRL of TCF in farmed A. japonica.
Keywords
Anguilla japonica; eel; maximum residue level; pharmacokinetic; trichlorfon;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zhang, Q. & Li, X. Pharmacokinetics and residue elimination of oxytetracycline in grass carp, Ctenopharyngodon idellus, Aquaculture 272, 140-145, 2007.   DOI
2 Brandal, P. O., Eg~d~us, E. Treatment of salmon llce (Lepeophtheirus salmonis Krayer, 1838) wlth Neguvon® - description of method and equipment. Aquaculture 18, no. 2: 183-188, 1979.   DOI
3 Yang, F., Yang, F., Wang, G., Kong, T., Wang, H., & Zhang, C. Effects of water temperature on tissue depletion of florfenicol and its metabolite florfenicol amine in crucian carp (Carassius auratus gibelio) following multiple oral doses. Aquaculture, 515, 734542, 2020.   DOI
4 Zhang, Yong, Meirong Huo, Jianping Zhou, and Shaofei Xie. Pksolver: An Add-in Program for Pharmacokinetic and Pharmacodynamic Data Analysis in Microsoft Excel. Computer methods and programs in biomedicine 99, no. 3. 306-14, 2010.   DOI
5 Woo, S. J., Lee, H. H., & Chung, J. K. Simultaneous determination of trichlorfon and dichlorvos residues in olive flounder (Paralichthys olivaceus) by liquid chromatography- mass spectrometry: validation and application to pharmacokinetics. RRJPPS, 5, 85-95, 2016.
6 Guimaraes, A.T.B., Silva de Assis, H.C. and Boeger, W. The effect of trichlorfon on acetycholinesterase activity and histopathology of cultivated fish Oreochromis niloticus. Ecotoxicol. Environ. Saf., 68, 57-62, 2007.   DOI
7 M. D. Ferrando, E. Sancho, and E. Andreu-Moliner, "Comparative acute toxicities of selected pesticides to Anguilla anguilla," Journal of Environmental Science and Health. Part B, vol. 26, no. 5-6, pp. 491-498, 1991.   DOI
8 Son, K.T., Jo, M.R., Oh, E.G., Mok, J.S., Kwon, J.Y., Lee, T.S., Song, K.C., Kim, P.H. and Kwon, H.J. Residues of ampicillin and amoxicillin in olive flounder Paralichthys olivaceus following oral administration, Kor J Fish Aquat Sci, 44(5): 464-469, 2011.   DOI
9 Treves-Brown, K.M. Tetracyclines. In Applied Fish Pharmacology, pp. 64 82. Kluwer Academic Publishers, Boston, 2000.
10 WHO. Antimicrobial Use in Aquaculture and Antimicrobial Resistance. World Health Organization.
11 Yonar, M Enis, Serpil Mise Yonar, Aysegul Pala, Sibel Silici, and Naim Saglam. Trichlorfon-Induced Haematological and Biochemical Changes in Cyprinus Carpio: Ameliorative Effect of Propolis. Diseases of aquatic organisms 114, no. 3: 209-16, 2015.   DOI
12 Woo, S. J. Drug metabolism system associated with the exposure of benzo[a]pyrene and trichlorfon in rockfish (Sebastes schlegelii). dissertation, Pukyong National University Graduate School, 2019.
13 Xu, N., Li, M., Fu, Y., Zhang, X., Dong, J., Liu, Y., Zhou, S., Ai, X., & Lin, Z. Effect of temperature on plasma and tissue kinetics of doxycycline in grass carp (Ctenopharyngodon idella) after oral ad-ministration. Aquaculture, 511, 734204, 2019.   DOI