• Fisheries and Aquatic Sciences
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• 제20권7호
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• pp.9.1-9.13
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• 2017

Background: Metal-responsive transcription factor-1 (MTF-1) is a key transcriptional regulator playing crucial roles in metal homeostasis and cellular adaptation to diverse oxidative stresses. In order to understand cellular pathways associated with metal regulation and stress responses in Pacific abalone (Haliotis discus hannai), this study was aimed to isolate the genetic determinant of abalone MTF-1 and to examine its expression characteristics under basal and experimentally stimulated conditions. Results: The abalone MTF-1 shared conserved features in zinc-finger DNA binding domain with its orthologs; however, it represented a non-conservative shape in presumed transactivation domain region with the lack of typical motifs for nuclear export signal (NES) and Cys-cluster. Abalone MTF-1 promoter exhibited various transcription factor binding motifs that would be potentially related with metal regulation, stress responses, and development. The highest messenger RNA (mRNA) expression level of MTF-1 was observed in the testes, and MTF-1 transcripts were detected during the entire period of embryonic and early ontogenic developments. Abalone MTF-1 was found to be Cd inducible and highly modulated by heat shock treatment. Conclusion: Abalone MTF-1 possesses a non-consensus structure of activation domains and represents distinct features for its activation mechanism in response to metal overload and heat stress. The activation mechanism of abalone MTF-1 might include both indirect zinc sensing and direct de novo synthesis of transcripts. Taken together, results from this study could be a useful basis for future researches on stress physiology of this abalone species, particularly with regard to heavy metal detoxification and thermal adaptation.

• 한국패류학회지
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• 제20권1호
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• pp.17-26
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• 2004

In present study, attempts were made to preserve abalone (Haliotis discus hannai) sperm in liquid form at low temperature, to evaluate the effect of various diluents in short-term storage on sperm, and cryopreservation procedures were optimized for the cryoprotectants and freezing rates, as well as the motility, survival rate, and the ultrastructural changes of sperm after short-term storage and cryopreservation were observed. The abalone sperm reached maximum motility until about 4 min after activation. The motility was constant for about 16 min, after which it dropped gradually, and about 50 min later all motility ceased. In Hanks' balanced salt solution (HBSS, 300 and 400 mOsmol/kg) and 150, 250 and 350 mOsmol/k artificial seawater (ASW), the sperm was immotile. After 100% ASW was added, motility of those sperm, which are in 300, 400 mOsmol/kg HBSS, 250, 350 mOsmol/kg ASW, could be again restored incompletely. Sperm motility can be maintained for 20 days of cold storage only in ASW of 850 and 1200 mOsmol/kg. A high motility index of 3.5-4.5 was observed for the first 8 days in 850 and 950 mOsmol/kg ASW. In other diluents sperm motility was constant less than 10 days, and the motility index was obviously lower than that of sperm in 850 and 1200 mOsmol/kg ASW. After 20 days of cold storage, survival rates of 10.2%-20.7% were obtained in ASW and 300 mOsmol/kg HBSS, and that in 400 HBSS (65.3%) was significantly higher than others. The constant period of sperm motility stored in 850 mOsmol/kg ASW was obviously longer than that in 1200 mOsmol/kg ASW after 6 days of storage. The sperm plunged into liquid nitrogen all died except that sperm using 15% glycerol as cryoprotectant restored 10.4% of motility. The highest motility index (3.4) was obtained with 5% glycerol and freezing procedure: $-50^{\circ}C$/min from $20^{\circ}C$ to $-80^{\circ}C$.

• 한국수산과학회지
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• 제36권6호
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• pp.591-595
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• 2003

Settlement of larvae, growth, and survival of juvenile abalone (Haliotis discus hannai) were examined after feding of 5 species of benthic diatoms (Cocconeis suctellum, Navicula sp Nitzschia longissima Bacillavia paxillifera, Licmophora flabellata). All of the benthic diatoms were isolated from natural populations on plastic plates for the abalone. The settlement of larvae, shell growth, and survival rate of juvenile abalone varied by diatoms species. The highest settlement rate of larvae was $43.1\%$ with the Cocconeis suctellum diet. Daliy growth rate reached a maximum at 64.1 ${\mu}m/day$ with the Navicula sp. diet and a minimum at 22.4 ${\mu}m/day$ with the Licmophora flabellata diet. Survival rate of the juvenile abalone was highest at $62.0\%$ with the Navicula sp., Cocconeis suctellum , and Nitzschia longissima diets. Survival rate of the Juvenile abalone was significantly higher than the control group with mixed diatom population diets (P<0.05). Therefore, diatom . species composition in diets can be a controling factor for the settlement, growth, and survival rates of Juvenile abalone.

• 수산경영론집
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• 제46권1호
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• pp.1-13
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• 2015

This study is aimed comparative analysis for business performance of land-based ezo abalone (Haliotis discus hannai) seed farms by standardizing cost structures by region and farming size. The result of survey on average farming incomes by region showed that farming incomes in Haenam and Jindo regions were much higher than those in other regions. Followed by Wando region, incomes in other regions were analyzed to be relatively lower. It is shown that farming incomes became higher as farm size increased. Farming incomes per unit size which were modified from farming incomes by region were highest in Jindo region, followed by East sea region, Wando and Haenam regions. Incomes in Jeju and Yeosu regions were analyzed to be relatively lower. Results on farming incomes per unit size ($m^2$) showed that both farming incomes and profits became higher as size increased. It implies that a scale effectiveness might exist in case of land-based abalone culture system. Impacts of major factors on farming profits and returns on investment in abalone seed aquaculture are summarized as follow. First, only if the survival rate increased by 10% with improvement of component ratio, variable effects became largest. In variable effects of other factors, a variation in Jindo region was largest and on the other hand, a variation in Yeosu region was shown to be smallest.

• 한국패류학회지
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• 제32권4호
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• pp.249-254
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• 2016

북방전복을 대상으로 성별에 따른 성장형질의 변이를 이용한 선발육종의 가능성 여부를 확인하고자, 실제로 암수 1:1 수정을 통하여 10가계를 생산 관리하여 성별에 따른 성장형질의 표현형 측정과 유전모수를 추정하였다. 그 결과 성비율은 1:1로 일반적 전복류의 성비와 같은 결과를 나타내었으며, 성별에 따른 성장은 모든 성장형질에 있어 암컷이 수컷보다 유의적으로 큰 것으로 나타났다 (p < 0.05). 이러한 결과는 선발을 할 때 성별에 따른 성장차이를 이용하여 양식전복의 성장률 향상을 할 수 있는 가능성을 시사하고 있다.

• 환경생물
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• 제31권4호
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• pp.458-470
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• 2013

Haliotis discus, a useful abalone of herbivorous gastropod, shows feeding preference to marine algae depending upon their growth stage and recognition of taste. This study was carried out to investigate this abalone's algal preferences and the presence of feeding stimulants. In single-choice experiments the small (S) group generally preferred Ulva pertusa (Chlorophyta), whereas the medium (M) and large (L) group preferred both Laminaria japonica (Phaeophyta) and Undaria pinnatifida (Phaeophyta). In multi-choice experiments using 4 algal species of L. japonica, U. pertusa, U. pinnatifida and Ecklonia cava (Phaeophyta), the results were same as in the single-choice experiments; the S group preferred U. pertusa the most, while the M and L group preferred both U. pinnatifida and L. japonica. However E. cava was not preferred by any groups. In order to examine the presence of feeding stimulant, chemical compounds from algae used as feed were isolated and identified. The abalone responded to water soluble matters of L. japonica, U. pinnatifida and U. pertusa, but those of E. cava and Sargassum sagamianum (Phaeophyta) were not attractive to them. In feeding stimulant experiments using fat soluble matters, the S group preferred the fat soluble matter of U. pertusa the most, while the M group and the L group preferred those of U. pertusa and U. pinnatifida, and those of L. japonica, respectively. However the fat soluble matter of S. sagamianum was not attractive to the abalone. The results of feeding stimulant experiments were same as those of single-choice or multi-choice experiments, which showed that compound lipids in fat soluble matter might act as feeding-stimulant.

• 한국수산과학회지
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• 제32권4호
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• pp.391-394
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• 1999

참전복용 배합사료에 비타민 및 미네랄 혼합물의 첨가 효과를 조사하기 위해 실용적인 배합비에 비타민 혼합물 ($0\~2\%$)과 미네랄 혼합물 ($0\~6\%$)을 각각 달리 첨가한 사료로 평균체중 100mg의 참전복 치패를 3반복으로 4개월간 사육실험한 결과, 최종 평균 체중, 증체율, 가식부 중량, 체중에 대한 가식부 중량 비, 각장, 각폭 및 각장에 대한 체중 비의 값들은 사료의 비타민 및 미네랄에 영향을 받지 않았다 (P>0.05). 또한, 생존율과 가식부의 일반성분은 실험구간에 유의적은 차이를 보이는 실험구가 있기는 하였으나, 사료의 비타민이나 미네랄의 첨가수준에 따른 차이는 아니었다. 이상의 결과로부터 본 실험의 사료 배합비에는 비타민 혼합물과 미네랄 혼합물은 별도로 보충하지 않아도 될 것으로 판단되며, 그 만큼 사료단가를 절감시킬 수 있을 것으로 기대된다.

• Fisheries and Aquatic Sciences
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• 제24권3호
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• pp.129-137
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• 2021

Dietary protein requirements of abalone (Haliotis discus) depending on abalone size were determined and compared. One thousand and fifty small abalone (initial weight of 2.7 g) and five hundred forty large one (initial weight of 16.0 g) were distributed into 15 and 18 containers in Trial 1 and 2, respectively. Five and six experimental diets containing crude protein level from 20% to 40% and 20% to 45% with 5% increment of protein level for the small and large abalone were prepared and referred to as the CP20, CP25, CP30, CP35, CP40, and CP45 diets, respectively. The experimental diets were fed to abalone for 16 weeks in Trials 1 and 2. Specific growth rate (SGR) of the small abalone fed the CP20 diet was lower compared to that of abalone fed all other diets in Trial 1. Growth performance (weight gain and SGR) of the large abalone fed the CP30, CP35, and CP40 diets were greater than that of abalone fed the CP20, CP25, and CP45 diets in Trial 2. Dietary protein requirements were estimated to be 33.0% and 33.5% for the small and large abalone based on the 2^nd order polynomial analysis, respectively. Dietary protein requirements for the small abalone grown from 2.7 g to 7.4 g and the large one grown from 16 g to 21 g were estimated to be 33.0% and 33.5%, respectively. Size differences in abalone did not affect dietary protein requirement under this experimental conditions.

• 한국미생물·생명공학회지
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• 제50권1호
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• pp.22-30
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• 2022

Haliotis discus hannai called abalone, is the valuable marine mollusks and the by-products of abalone processing are viscera. Brownish abalone male viscera (AMV), which have not been reported as having anti-inflammatory effects, was extracted with acetone and fractionated by different six acetone/hexane ratios (0, 10, 20, 30, 40, and 100%) using a silica column via in vitro ABTS and DPPH radical and nitric oxide (NO) production assay-guided fractionation. Among the fractions, the acetone/hexane ratio 40%, A40 exhibited the most potent radical scavenging activities and inhibition of lipopolysaccharide (LPS)-induced NO production without cytotoxicity. A40 inhibited LPS-induced intracellular reactive oxygen species (ROS) production in a dose-dependent manner. Western blot analysis revealed that A40 down-regulated the activation of NF-κB, MAPK (ERK 1/2, p-38, and JNK), and inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. Moreover, this fraction inhibited the generation of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. These results suggested that AMV containing A40 with anti-inflammatory and anti-oxidantive effects, is the effective therapeutic and functional material for treating inflammatory disorders.

• 한국수산과학회지
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• 제56권4호
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• pp.541-548
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• 2023

Behavior serves as an important indicator of the effects of environmental stressors on organisms. In this study, one-year-old abalone Haliotis discus hannai (15.1±0.8 mm) were used and each 0.34 m2 tank was stocked with 100, 150, 200, 250, and 300 juvenile abalone. After 328 days, we found that the low stocking density treatment showed significantly higher growth, whereas the high stocking density treatment showed significantly lower growth. In addition, abalone showed a lower rate of movement, distance moved, and velocity at higher stocking densities, but only velocity differed significantly. In contrast, In the Supplied the feed tanks significant difference between the moving distance and velocity. When the two groups of different sizes were mixed in tank, the rate of movement, distance moned, and velocity showed significant differences between differently-sized indivisuals. Our study indicates that breeding abalone at high densities has a negative effect on their growth and survival, which is thought to primarily be caused by spatial competition, and secondarily by differences in body size, ultimately affecting growth and productivity.