Browse > Article
http://dx.doi.org/10.48022/mbl.2109.09005

Anti-Inflammatory Effects of Abalone (Haliotis discus hannai) Viscera via Inhibition of ROS Production in LPS-Stimulated RAW 264.7 Cells  

Shin, Tai-Sun (Division of Food and Nutrition, College of Human Ecology, Chonnam National University)
Choi, Kap Seong (Department of Food Science and Technology, College of Life Sciences and Natural Resources, Sunchon National University)
Chun, Jiyeon (Department of Food Science and Technology, College of Life Sciences and Natural Resources, Sunchon National University)
Kho, Kang-Hee (Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University)
Son, Seon Ah (Division of Food and Nutrition, College of Human Ecology, Chonnam National University)
Shim, Sun-Yup (Department of Food Science and Technology, College of Life Sciences and Natural Resources, Sunchon National University)
Publication Information
Microbiology and Biotechnology Letters / v.50, no.1, 2022 , pp. 22-30 More about this Journal
Abstract
Haliotis discus hannai called abalone, is the valuable marine mollusks and the by-products of abalone processing are viscera. Brownish abalone male viscera (AMV), which have not been reported as having anti-inflammatory effects, was extracted with acetone and fractionated by different six acetone/hexane ratios (0, 10, 20, 30, 40, and 100%) using a silica column via in vitro ABTS and DPPH radical and nitric oxide (NO) production assay-guided fractionation. Among the fractions, the acetone/hexane ratio 40%, A40 exhibited the most potent radical scavenging activities and inhibition of lipopolysaccharide (LPS)-induced NO production without cytotoxicity. A40 inhibited LPS-induced intracellular reactive oxygen species (ROS) production in a dose-dependent manner. Western blot analysis revealed that A40 down-regulated the activation of NF-κB, MAPK (ERK 1/2, p-38, and JNK), and inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2. Moreover, this fraction inhibited the generation of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. These results suggested that AMV containing A40 with anti-inflammatory and anti-oxidantive effects, is the effective therapeutic and functional material for treating inflammatory disorders.
Keywords
Haliotis discus hannai; abalone male viscera; ROS; NF-${\kappa}B$; MAPK; proinflammatory cytokines;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kresge N, Vacquier VD, Stout CD. 2001. Abalone lysin: the dissolving and evolving sperm protein. Bioessays 23: 95-103.   DOI
2 Urban MK. 2000. COX-2 specific inhibitors offer improved advantages over traditional NSAIDs. Orthopedics 23: S761-764.   DOI
3 Yangthong M, Hutadilok-Towatana N, Phromkunthong W. 2009. Antioxidant ac- tivities of four edible seaweeds from the southern coast of Thailand. Plant Foods Hum. Nutr. 64: 218-223.   DOI
4 Marks-Konczalik J, Chu SC, Moss J. 1998. Cytokine-mediated transcriptional induction of the human inducible nitric oxide synthase gene requires both activator protein 1 and nuclear factor κB-binding sites. J. Biol. Chem. 273: 22201-22208.   DOI
5 Ferrero LM, Nielsen OH, Andersen PS, Girardin SE. 2007. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1β generation. Clin. Exp. Immunol. 147: 227-235.   DOI
6 Duque AG, Descoteaux A. 2014. Macrophage cytokines: involvement in immunity and infectious diseases. Front. Immunol. 5: 491.   DOI
7 Torres M, Forman HJ. 2003. Redox signaling and the MAP kinase pathways. Biofactors 17: 287-296.   DOI
8 Zhang RJ, Shi Y, Zheng J, Mao XM, Liu ZY, Chen QX, et al. 2018. Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation. Int. J. Biol. Macromol. 106: 587-595.   DOI
9 Chen Z, Hagler J, Palombella VJ, Melandri F, Scherer D, Ballard D, et al. 1995. Signal-induced site-specific phosphorylation targets IκBα to the ubiquitinproteasome pathway. Genes Dev. 9: 1586-1597.   DOI
10 Schonthaler HB, Guinea-Viniegra J, Wagner EF. 2011. Targeting inflammation by modulating the Jun/AP-1 pathway. Ann. Rheumat. Dis. 70: i109-i112.   DOI
11 Bogdan C, Rollinghoff M, Diefenbach A. 2000. Reactive oxygen and nitrogen intermediates in innate and specific immunity. Curr. Opin. Immunol. 12: 64-76.   DOI
12 Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. 2018. Inflammatory response and inflammation-associated diseases in organs. Oncotarget 9: 7204-7218.   DOI
13 Zhang Q, Zhu B, Li Y. 2017. Resolution of cancer-promoting inflammation: A new approach for anticancer therapy. Front. Immunol. 8: 71.   DOI
14 Guha M, Mackman N. 2001. LPS induction of gene expression in human monocytes. Cell. Signal. 13: 85-94.   DOI
15 Medzhitov R, Horng T. 2009. Transcriptional control of the inflammatory response. Nat. Rev. Immunol. 9: 692-703.   DOI
16 Kaur G, Sharma A, Bhatnagar A. 2021. Role of oxidative stress in pathophysiology of rheumatoid arthritis: insights into NRF2-KEAP1 signalling. Autoimmunity 54: 385-397.   DOI
17 Andus T, Geiger T, Hirano T, Kishimoto T, Heinrich PC. 1988. Action of recombinant human interleukin 6, interleukin 1β and tumor necrosis factor α on the mRNA induction of acute-phase proteins. Eur. J. Immunol. 18: 739-746.   DOI
18 Guo S, Wang J, He C, Wei H, Ma Y, Xiong H. 2020. Preparation and antioxidant activities of polysaccharides obtained from abalone viscera by combination of enzymolysis and multiple separation methods. J. Food Sci. 85: 4260-4270.   DOI
19 Zhou DA, Ma DD, Zhao J, Wan XL, Tong L, Song S, et al. 2016. Simultaneous recovery of protein and polysaccharide from abalone (Haliotis discus hannai Ino) gonad using enzymatic hydrolysis method. J. Food Process. Preserv. 40: 119-130.   DOI
20 Gong F, Chen MF, Chen J, Li C, Zhou C, Hong P, et al. 2019. Boiled abalone byproduct peptide exhibits anti-tumor activity in HT1080 Cells and HUVECs by suppressing the metastasis and angiogenesis in Vitro. J. Agric. Food Chem. 67: 8855-8867.   DOI
21 Kwon MJ, Kim B, Lee YS, Kim TY. 2012. Role of superoxide dismutase 3 in skin infammation. J. Dermatol. Sci. 67: 81-87.   DOI
22 Molofsky AB, Savage AK, Locksley RM. 2015. Interleukin-33 in tissue homeostasis, injury, and inflammation. Immunity 42: 1005-1019.   DOI
23 Murakami A, Ohigashi H. 2007. Targeting NOX, INOS and COX-2 in inflammatory cells: chemoprevention using food phytochemicals. Int. J. Cancer 121: 2357-2363.   DOI
24 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.   DOI
25 Liu T, Zhang L, Joo D, Sun SC. 2017. NF-κB signaling in inflammation. Signal Transduct. Target Ther. 2: e17023.
26 Suleria HAR, Masci PP, Gobe GC, Osborne SA. 2017. Therapeutic potential of abalone and status of bioactive molecules: A comprehensive review. Crit. Rev. Food Sci. Nutr. 57: 1742-1748.   DOI
27 Proestos C, Lytoudi K, Mavromelanidou O, Zoumpoulakis P, Sinanoglou V. 2013. Antioxidant capacity of selected plant extracts and their essential oils. Antioxidants 2: 11-22.   DOI
28 Raghav SK, Gupta B, Shrivastava A, Das HR. 2007. Inhibition of lipopolysaccharide-inducible nitric oxide synthase and IL-1β through suppression of NF-κB activation by 3-(1'-1'-dimethylallyl)-6-hydroxy-7-methoxy-coumarin isolated from Ruta graveolens L. Eur. J. Pharmacol. 560: 69-80.   DOI
29 Bjelakovic G, Nikolova D, Gluud C. 2014. Antioxidant supplements and mortality. Curr. Opin. Clin. Nutr. Metab. Care 17: 40-44.
30 Cobb MH, Goldsmith EJ. 1995. How MAP kinase are regulated. J. Biol. Chem. 270: 14843-14846.   DOI
31 Dinarello CA. 2009. Immunological and inflammatory functions of the interleukin-1 family. Annu. Rev. Immunol. 27: 519-550.   DOI
32 Finkel T. 2011. Signal transduction by reactive oxygen species. J. Cell Biol. 194: 7-15.   DOI
33 Sun LD, Wang F, Dai F, Wang YH, Lin D, Zhou B. 2015. Development and mechanism investigation of a new piperlongumine derivative as a potent anti-inflammatory agent. Biochem. Pharmacol. 95: 156-169.   DOI
34 Marin DP, Bolin AP, Campoio TR, Guerra BA, Otton R. 2013. Oxidative stress and antioxidant status response of handball athletes: Implications for sport training monitoring. Int. Immunopharmacol. 17: 462-470.   DOI
35 Baggiolini M, Clark-Lewis I. 1992. Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett. 307: 97-101.   DOI