• Title/Summary/Keyword: Haliotis

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Phylogenetic Study of Genus Haliotis in Korea by Cytochrome c Oxidase Subunit 1 and RAPD Analysis (Cytochrome c oxidase subunit 1과 RAPD 분석에 의한 한국 전복속의 계통 연구)

  • Seo, Yong Bae;Kang, Sung Chul;Choi, Seong Seok;Lee, Jong Kyu;Jeong, Tae Hyug;Lim, Han Kyu;Kim, Gun-Do
    • Journal of Life Science
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    • v.26 no.4
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    • pp.406-413
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    • 2016
  • Abalones are gastropod mollusks belonging to the genus Haliotis. Pacific abalones are regarded as a very important marine gastropod mollusk in Korea, Japan, China, and also in food industries around the world. In Korea, 6 species of abalone have been reported to occur along the coasts: Haliotis discus hannai, Haliotis discus discus, Haliotis madaka, Haliotis gigantea, Haliotis diversicolor supertexta, and Haliotis diversicolor diversicolor. This study was performed to discriminate the genetic variances by the partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) genes and random amplified polymorphic DNA (RAPD) analysis against four species of Pacific abalone (H. discus hannai, H. discus, H. madaka, H. gigantea). COI gene is reasonably well conserved and has been sequenced in various invertebrate taxa. The RAPD analysis technique is a relatively simple and low cost method that allows differentiation of taxa without the need to know their genomes. In this study, we investigated the genetic diversity, phylogenetic relationships within each species. The COI and RAPD analysis were able to distinguish between H. gigantea and the other three species. However, these analysis methods were inadequate to distinguish between H. discus and H. madaka. These results are believed to be able to provide a basis data for future hybrid breeding research by defining the genetically closely related four species of abalone, which is to develop new hybrid abalone for export using hybrid breeding.

전복 드링크 제조를 위한 양식전복 열성패의 원료학적 성분특성

  • 오광수;김영아;김진수;강수태
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.164-165
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    • 2001
  • 현재 우리나라에 서식하는 전복은 말전복(Haliotis gigantea), 까막전복(Haliotis discus), 참전복(Haliotis discus hannai), 시볼트전복(Haliotis sieboldii), 오분자기(Haliotis diversicolor superfexta), 둥근전복(Haliotis diversicolor diversicolor) 등 6종이 있고, 이 중 참전복은 가장 맛이 좋아 주로 날 것으로 또는 건제품 및 죽의 형태로 소비되는 고가의 중요한 연안 수산물이다. 특히, 전복은 식품학적 측면으로 볼 때, 오래 전부터 우리나라, 중국 및 일본 등지에서 상당히 귀하게 여기는 기능성 수산물이어서 시장 가치가 매우 높다고 할 수 있다. (중략)

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Molecular and Physiological Aspects of Breeding Program for Development of Hybrids between Abalones Distributed in the Coast of Korea (한국산 전복을 이용한 교잡종 개발)

  • Lee, Jong Kyu;Seo, Yong Bae;Kim, Gun-Do;Lim, Han Kyu
    • Journal of Life Science
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    • v.26 no.10
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    • pp.1218-1223
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    • 2016
  • Abalones are gastropod mollusks belonging to the genus Haliotis. Fishery products are continuously declining worldwide while abalone production from aquatic farms has considerably increased. Although abalones are regarded as very important marine resources and considered to have big potential in sea-food farming industry around world, the slow growth rate of Pacific abalones is considered to be one of the most serious problems. For the genetic improvements in cultured abalone, advances in various breeding techniques for abalone have been reached through the introduction of selection, crossbreeding, hybridization, and polyploidy in several commercially important abalone species. Six species of abalone have been reported to be distributed along the coasts of Korea: Haliotis discus hannai, Haliotis discus discus, Haliotis madaka, Haliotis gigantea, Haliotis diversicolor diversicolor, and Haliotis diversicolor supertexta. The hybridization between these abalones may be one of the advanced technologies, and the preliminary experiments of interspecific hybrids between abalone species distributed in northern pacific areas including Korea, China, and Japan have been conducted. In this study, we reviewed the phylogenetic relationship of northern pacific abalone species which have the potential traits for aquaculture in Korea and their identifications. We also examined the development of molecular markers and some other aspects of the genetic approaches for successful development of hybrids.

Construction of web-based Database for Haliotis SNP (웹기반 전복류 (Haliotis) SNP 데이터베이스 구축)

  • Jeong, Ji-Eun;Lee, Jae-Bong;Kang, Se-Won;Baek, Moon-Ki;Han, Yeon-Soo;Choi, Tae-Jin;Kang, Jung-Ha;Lee, Yong-Seok
    • The Korean Journal of Malacology
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    • v.26 no.2
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    • pp.185-188
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    • 2010
  • The Web-based the genus Haliotis SNP database was constructed on the basis of Intel Server Platform ZSS130 dual Xeon 3.2 GHz cpu and Linux-based (Cent OS) operating system. Haliotis related sequences (2,830 nucleotide sequences, 9,102 EST sequences) were downloaded through NCBI taxonomy browser. In order to eliminate vector sequences, we conducted vector masking step using cross match software with vector sequence database. In addition, poly-A tails were removed using Trimmest software from EMBOSS package. The processed sequences were clustered and assembled by TGICL package (TIGR tools) equipped with CAP3 software. A web-based interface (Haliotis SNP Database, http://www.haliotis.or.kr) was developed to enable optimal use of the clustered assemblies. The Clustering Res. menu shows the contig sequences from the clustering, the alignment results and sequences from each cluster. And also we can compare any sequences with Haliotis related sequences in BLAST menu. The search menu is equipped with its own search engine so that it is possible to search all of the information in the database using the name of a gene, accession number and/or species name. Taken together, the Web-based SNP database for Haliotis will be valuable to develop SNPs of Haliotis in the future.

Machine Learning SNP for Classification of Korean Abalone Species (Genus Haliotis) (전복류(Genus Haliotis)의 분류를 위한 단일염기변이 기반 기계학습분석)

  • Noh, Eun Soo;Kim, Ju-Won;Kim, Dong-Gyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.4
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    • pp.489-497
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    • 2021
  • Climate change is affecting the evolutionary trajectories of individual species and ecological communities, partly through the creation of new species groups. As population shift geographically and temporally as a result of climate change, reproductive interactions between previously isolated species are inevitable and it could potentially lead to invasion, speciation, or even extinction. Four species of abalone, genus Haliotis are present along the Korean coastline and these species are important for commercial and fisheries resources management. In this study, genetic markers for fisheries resources management were discovered based on genomic information, as part of the management of endemic species in response to climate change. Two thousand one hundred and sixty one single nucleotide polymorphisms (SNPs) were discovered using genotyping-by-sequencing (GBS) method. Forty-one SNPs were selected based on their features for species classification. Machine learning analysis using these SNPs makes it possible to differentiate four Haliotis species and hybrids. In conclusion, the proposed machine learning method has potentials for species classification of the genus Haliotis. Our results will provide valuable data for biodiversity conservation and management of abalone population in Korea.

Red Pigment Producing Serratia marcescens Isolated from Abalone (Haliotis discus) (전복(Haliotis discus)에서 분리한 Serratia marcescens가 생산하는 적색 색소의 항균활성)

  • Shin, YuJin;Kang, Chang-Ho;So, Jae-Seong
    • KSBB Journal
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    • v.31 no.4
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    • pp.214-218
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    • 2016
  • Serratia marcescens characterized by the ability to produce red pigments inhabits various ecological niches. A strain Serratia marcescens PYU was isolated from abalone (Haliotis discus) collected at the West Sea in Korea. The isolated strain was gram-negative, motile, rods like coccus, oxidase-negative, and catalase-positive; and formed red pigment. S. marcescens PYU was grown in the presence of 0~10% (w/v) NaCl, at pH 4~9, and at $10{\sim}40^{\circ}C$. The strain PYU produced red pigment, and the extracted pigment showed antibacterial activity against Streptococcus iniae and Lactococcus garviae which has been known as an important fish pathogens. Further studies are underway to elucidate the direct relationship between the red pigment and antibacterial activity.

Feces Production Rate of Abalone (Haliotis discus hannai) fed Undavia pinnatifida (미역 (Undaria pinnatifida)을 섭취 한 참전복(Haliotis discus hannai)의 분 배출률)

  • Park, Jeong-Hwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.6
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    • pp.353-358
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    • 2005
  • The present study investigated the feces production rate and fecal nitrogen production ratio of abalone, Haliotis discus hannai fed seaweed, Undaria pinnatifida. The abalone, with shell lengths of 3 cm, 5 cm, and 7 cm, were tested at temperatures of $12^{\circ}C$, $16^{\circ}C$, and $20^{\circ}C$ in a semi-recirculating aquaculture system. Under the given experimental conditions, the weight specific feces production rate (FPw) of the abalone increased with the decrease of shell length and the increase of temperature. The relationship of the FPw was. $FPw=-3.092+206.573/T - 1916.8/T^2+0.141L -0.037L^2(r^2=0.9412)$. In addition the abalone produced 17.8-22.5% of fecal material against ingested seaweed, Undaria pinnatifida, and $18.4-22.4\%$ of nitrogen ingested total nitrogen.

Influence of elevated temperatures on the physiological response of hemolymph from two species of abalone, Haliotis gigantea and Haliotis discus discus (Reeve, 1846) (수온 증가에 따른 말전복, Haliotis gigantea과 둥근전복, Haliotis discus discus (Reeve, 1846) hemolymph의 생리학적 변화)

  • Min, Eun-Young;Kim, Shin-Hu;Hwang, In-Ki;Kim, Kyeong-Wook;Park, Bo-Mi;Lee, Jung Sick;Kang, Ju-Chan
    • The Korean Journal of Malacology
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    • v.31 no.3
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    • pp.187-194
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    • 2015
  • This study was conducted to examine the effects of alterations in water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Haliotis gigantea and H. discus discus. The abalone were exposed to various WT; 18, 20, 22, 24, 26 and $28^{\circ}C$ for 96 hours. In biochemical factors, total-protein (TP), glucose, magnesium (Mg), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not significant changes in hemolymph of H. gigantea and H. discus discus. But calcium was significantly increased by high WT (${\geq}24^{\circ}C$). In immunological factor, The phenoloxidase (PO) activity was decreased in hemolymph of H. gigantea and H. discus discus exposed to high temperature (${\geq}22^{\circ}C$) compared to the control (P < 0.05). Whereas alkaline phosphatase (ALP) was not significantly changed. These results suggested that high temperature adversely affects the immunity of H. gigantea and H. discus discus.

Phylogenetic Study of Genus Haliotis In Korea by Internal Transcribed Spacer Sequence (ITS) (ITS에 의한 한국내 전복 속 분류군의 유전적 계통분류학적 연구)

  • Huh, Man-Kyu;Kim, Jung-Ho;Moon, Du-Ho
    • Journal of Life Science
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    • v.19 no.8
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    • pp.1003-1008
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    • 2009
  • Abalone (genus Haliotis) is a woody species with a long life span that is primarily distributed throughout the world, including Asia. This species is regarded as a very important marine gastropod mollusk in Korea and China, and also in food industries around the world. We evaluated a representative sample of the five species with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within the genus. Aligned nucleotide sequences of the length of the 5.8S subunit of all taxa of Haliotis were found to constant of 160 bp nucleotides. However, aligned nucleotide sequences of the length of ITS1 were varied within genus Haliotis, varying from 272 in H. diversicolor aquatilis to 292 in H. discus hannai. Aligned nucleotide sequences of the length of ITS2, especially, vary from 722 in H. diversicolor aquatilis to 752 in H. sieboldii. Total alignment length is 763 positions, of which 78 are parsimony-informative, 57 variable but parsimony-uninformative, and 459 constant characters. H. discus hannai was similar to H. discus, while H. diversicolor aquatilis was more distinct. ITS analysis may be useful in germ-plasm classification several taxa of genus Haliotis.