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http://dx.doi.org/10.5352/JLS.2009.19.8.1003

Phylogenetic Study of Genus Haliotis In Korea by Internal Transcribed Spacer Sequence (ITS)  

Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
Kim, Jung-Ho (Biological Education, Busan National University)
Moon, Du-Ho (Biological Education, Busan National University)
Publication Information
Journal of Life Science / v.19, no.8, 2009 , pp. 1003-1008 More about this Journal
Abstract
Abalone (genus Haliotis) is a woody species with a long life span that is primarily distributed throughout the world, including Asia. This species is regarded as a very important marine gastropod mollusk in Korea and China, and also in food industries around the world. We evaluated a representative sample of the five species with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within the genus. Aligned nucleotide sequences of the length of the 5.8S subunit of all taxa of Haliotis were found to constant of 160 bp nucleotides. However, aligned nucleotide sequences of the length of ITS1 were varied within genus Haliotis, varying from 272 in H. diversicolor aquatilis to 292 in H. discus hannai. Aligned nucleotide sequences of the length of ITS2, especially, vary from 722 in H. diversicolor aquatilis to 752 in H. sieboldii. Total alignment length is 763 positions, of which 78 are parsimony-informative, 57 variable but parsimony-uninformative, and 459 constant characters. H. discus hannai was similar to H. discus, while H. diversicolor aquatilis was more distinct. ITS analysis may be useful in germ-plasm classification several taxa of genus Haliotis.
Keywords
Abalone; Haliotis; ITS; phylogenetic analysis;
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