• Molecules and Cells
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• 제44권2호
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• pp.101-115
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• 2021

The INO80 chromatin remodeling complex has roles in many essential cellular processes, including DNA replication. However, the mechanisms that regulate INO80 in these processes remain largely unknown. We previously reported that the stability of Ino80, the catalytic ATPase subunit of INO80, is regulated by the ubiquitin proteasome system and that BRCA1-associated protein-1 (BAP1), a nuclear deubiquitinase with tumor suppressor activity, stabilizes Ino80 via deubiquitination and promotes replication fork progression. However, the E3 ubiquitin ligase that targets Ino80 for proteasomal degradation was unknown. Here, we identified the C-terminus of Hsp70-interacting protein (CHIP), the E3 ubiquitin ligase that functions in cooperation with Hsp70, as an Ino80-interacting protein. CHIP polyubiquitinates Ino80 in a manner dependent on Hsp70. Contrary to our expectation that CHIP degrades Ino80, CHIP instead stabilizes Ino80 by extending its half-life. The data suggest that CHIP stabilizes Ino80 by inhibiting degradative ubiquitination. We also show that CHIP works together with BAP1 to enhance the stabilization of Ino80, leading to its chromatin binding. Interestingly, both depletion and overexpression of CHIP compromise replication fork progression with little effect on fork stalling, as similarly observed for BAP1 and Ino80, indicating that an optimal cellular level of Ino80 is important for replication fork speed but not for replication stress suppression. This work therefore idenitifes CHIP as an E3 ubiquitin ligase that stabilizes Ino80 via nondegradative ubiquitination and suggests that CHIP and BAP1 act in concert to regulate Ino80 ubiquitination to fine-tune its stability for efficient DNA replication.

• 한국지반환경공학회 논문집
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• 제12권9호
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• pp.47-54
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• 2011

본 연구에서는 NATM으로 시공되는 산악터널의 시공과정에서 실측된 여굴, 숏크리트의 리바운드 및 투입율에 대한 분석을 실시하였다. 터널굴착 직후 굴착단면에 대한 여굴 크기를 조사한 결과 평균 여굴의 크기는 약 28.5cm 이며, 허용 여굴의 약 260%의 값을 보였다. 숏크리트의 리바운드는 평균 약 7.2% 정도로 측정되었는데 이는 일반적인 설계규정인 15% 보다 작은 값이다. 여굴 및 숏크리트 타설 중에 발생한 리바운드로 인하여 터널공사에 실제로 투입된 숏크리트 물량은 설계대비 약 116.5%의 값을 보였다. 자료분석에 의하면 숏크리트의 설계대비 실제투입율은 RMR(Rock Mass Rating)이나 터널 표준지보패턴과는 어느 정도 상관관계가 있는 것으로 나타났으나, 이에 비해 여굴은 RMR 값이나 표준지보패턴과는 상관성이 낮은 것으로 분석되었다. 따라서 표준지보패턴만에 의해 허용 여굴의 크기를 규정하는 현행 터널설계기준에 대한 재검토가 필요할 것으로 판단된다. 본 연구 결과를 통하여 분석된 여굴 및 숏크리트 투입량에 대한 고찰결과를 보고하고자 한다.

• 한국안전학회지
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• 제36권3호
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• pp.31-39
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• 2021

In Korea, more than half of work-related fatalities have occurred on construction sites. To reduce such occupational accidents, safety inspection by government agencies is essential in construction sites that present a high risk of serious accidents. To address this issue, this study developed risk prediction models of serious accidents in construction sites using five machine learning methods: support vector machine, random forest, XGBoost, LightGBM, and AutoML. To this end, 15 proactive information (e.g., number of stories and period of construction) that are usually available prior to construction were considered and two over-sampling techniques (SMOTE and ADASYN) were used to address the problem of class-imbalanced data. The results showed that all machine learning methods achieved 0.876~0.941 in the F1-score with the adoption of over-sampling techniques. LightGBM with ADASYN yielded the best prediction performance in both the F1-score (0.941) and the area under the ROC curve (0.941). The prediction models revealed four major features: number of stories, period of construction, excavation depth, and height. The prediction models developed in this study can be useful both for government agencies in prioritizing construction sites for safety inspection and for construction companies in establishing pre-construction preventive measures.

• 복식문화연구
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• 제29권2호
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• pp.272-288
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• 2021

The purpose of this study is to classify the types of decoration techniques used in women's Chima and Jeogori in traditional Hanbok catalogs from 2010 to 2020, and to analyze the frequency of each type. The method of this study is as follows. This study first investigated the transition of modern Hanbok and decorative techniques by analyzing and classifying such work in previous studies. Based on this, the technique of decorating the Jeogori and Chima that appeared in the traditional Hanbok catalog of the study period was analyzed. The results of the study are as follows. In the case of Jeogori, in the first half of 2010, the decorative technique of a relatively large size was used, and the decorativeness tended to be strong. However, in the late 2010s, the number of decorative techniques used in Jeogori has decreased, and the size of the decorative technique has become smaller and more concise, leading to a tendency to understated decoration. In the case of Chima, techniques to express natural texture by processing threads or fabrics themselves were mainly used rather than techniques to add decoration to the surface, and techniques to express various surface texture tended to develop toward the late 2010s. The change in the decoration technique of Jeogori and Chima appears to be a combination of social, cultural, and economic factors such as a change in consumption culture and a reduction in the wedding market.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.54-61
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• 2021

This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.

• Nuclear Engineering and Technology
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• 제54권8호
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• pp.3051-3058
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• 2022

In the present work, untreated Iraqi sand with grain sizes varied between 100 and 200 ㎛ was used to produce a colored glass sample that has shielding features against the low gamma-ray energy. Therefore, a weight of 70-60 wt % sand was mixed with 9-14 wt% B₂O₃, 8-10 wt% Na₂O, 4-6 wt% of CaO, 3-6 wt% Al₂O₃, in addition to 0.3% of Co₂O₃. After melting and annealing the glass sample, the X-ray diffraction spectrometry was applied to affirm the amorphous phase of the fabricated glass samples. Moreover, the X-ray dispersive energy spectrometry was used to measure the chemical composition, and the MH-300A densimeter was applied to measure the fabricated sample's density. The Makishima-Makinzie model was applied to predict the mechanical properties of the fabricated glass. Besides, the Monte Carlo simulation was used to estimate the fabricated glass sample's radiation shielding capacity in the low-energy region between 22.1 and 160.6 keV. Therefore, the simulated linear attenuation coefficient changed between 10.725 and 0.484 cm^-1, raising the gamma-ray energy between 22.1 and 160.6 keV. Also, other shielding parameters such as a half-value layer, pure lead equivalent thickness, and buildup factors were calculated.

• 한국엔터테인먼트산업학회논문지
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• 제14권2호
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• pp.41-50
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• 2020

본 연구는 연출자 및 감독에 의해 절제되는 콘티에 의해 분할된 연기에서가 아닌 프레임 속에 즉흥연기를 통해 배우의 감성과 템포 리듬이 분장한 롱샷의 연기와 풀샷 중심의 무대 연기가 내면적 서스펜스나 극 중 어떻게 표현되어 관객에게 전달할지 미적 반응을 분석 연구하고 연기 속에 나타난 서스펜스의 내면적 반응을 분석하여 극을 효과적으로 표현함에 도움 될 것을 목적으로 한다. 연기의 유형과 형식 그리고 매체는 앞으로도 더 다양해질 것이다. 그러나 다행히도 다매체시대에서 변하지 말아야 할 인간의 정신적 소양, 살아가는 동안 지켜야 할 정신들이 내면 깊이 숨어 있는 그 무엇인가의 희망이 아닌가 싶다. 본 연구는 연기가 가식이든 진실이든 그 형식이 중요한 것이 아니라 인간의 문화 정신세계의 풍요로움을 전달하는 전도사가 되는 것이라 하겠다. 향후 배우들의 만족 패턴과 연기 프로그램을 조사·연구하여 패러다임을 구축하고 연기의 효과적인 발전 방안을 분석, 연구, 돌출하고 그 방안을 토대로 연기의 근본적인 발전에 대한 방향을 제시하고자 한다.

• Safety and Health at Work
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• 제13권3호
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• pp.364-371
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• 2022

Background: Respiratory masks can provide healthcare workers with protection from biological hazards when they have good performance. There is a direct relationship between the visual specifications of a mask and its efficacy; thus, the aim of this study was to develop tools for qualitative assessment of the performance of masks used by healthcare workers. Methods: A mixed-methods design was used to develop a qualitative assessment tool for medical face masks (MFM) and particle filtering half masks (PFHM). The development of domains and items was undertaken using observation and interviews, the opinions of an expert panel, and a review of texts and international standards. The second phase evaluated the psychometric properties of tools. Finally, the validated Mask Qualitative Assessment Tools (MQAT) were used to assess six samples from 10 brands of the two types of masks. Results: MQAT-MFM and MQAT-PHFM shared 42 items across seven domains: "cleanliness," "design," "marking, labeling and packaging," "mask layers," "mask strap," "materials and construction," and "nose clip." MQAT-MFM included one additional item. MQAT-PHFM included another nine items associated with an eighth "Practical Performance" domain, and the valve version had another additional "Exhalation Valve" domain and six items. The evaluation indicated 80% compliance for MFM and 71% compliance for PFHM. "Marking, labeling and packaging" and "Layers" were associated with the least compliance in both types of masks and should be checked carefully for defining mask quality. Conclusion: MQAT can be used for immediate screening and initial assessment of MFM and PHFM through appearance, simple tools, and visual inspection.

• Geomechanics and Engineering
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• 제30권6호
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• pp.525-538
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• 2022

This paper presents a more general model for T-shaped combined footings that support two columns aligned on a longitudinal axis and each column provides an axial load and two orthogonal moments. This model can be applied to the following conditions: (1) without restrictions on its sides, (2) a restricted side and (3) two opposite sides restricted. This model considers the linear soil pressure. The recently published works have been developed for a restricted side and for two opposite sides restricted by Luévanos-Rojas et al. (2018a, b). The current model considers the uniform pressure distribution because the position of the resultant force coincides with the center of gravity of the surface of the footing in contact with the soil in direction of the longitudinal axis where the columns are located. This paper shows three numerical examples. Example 1 is for a T-shaped combined footing with a limited side (one column is located on the property boundary). Example 2 is for a T-shaped combined footing with two limited opposite sides (the two columns are located on the property boundary). Example 3 is for a T-shaped combined footing with two limited opposite sides, one column is located in the center of the width of the upper flange (b₁/2=L₁), and other column is located at a distance half the width of the strip from the free end of the footing (b₂/2=b-L₁-L). The main advantage of this work over other works is that this model can be applied to T-shaped combined footings without restrictions on its sides, a restricted side and two opposite sides restricted. It also shows the deficiencies of the current model over the new model.

• 현장농수산연구지
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• 제8권1호
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• pp.73-96
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• 2006

Today, producers of ginseng spray prohibited fertilizers or use excessive amount of fertilizers whereas distributers either claim that they are selling domestic ginseng when in fact they are selling imported ginseng, cheat on the age or weight of ginseng, or mix domestic and imported ginseng. In terms of quality in comparison with foreign ginseng, 90% of the people surveyed responded that domestic ginseng is more efficacious. When asked whether they would purchase foreign ginseng products when they become available in the market, only one of the respondents responded that he or she would purchase foreign ginseng whereas 50 respondents or 81% of the total surveyed responded that they wouldn't purchase foreign ginseng. About half of the respondents answered that when Chinese red ginseng, ginseng, or wild ginseng products cultivated with harmful fertilizers become available in the market, they would either reconsider purchasing ginseng products or stop purchasing ginseng products all together. In order to develop the ginseng industry 20 respondents (25%) chose the establishment of the standards, 33 respondents (42%) chose marking the place of origin, 15 respondents (19%) quality improvement, 7 respondents (9%) research on the efficacy, and 4 respondents (5%) said improvement on the storage methods as the important factors of the ginseng industry. Considering the fact that 77% of the respondents selected either the standardization or marking of the place of origin, the consumers seem to be demanding reliability in the distribution of ginseng products. Therefore, those people who work in the industry such as the producers, distributors and others should have a sense of mission to develop ginseng, which is a gift from God, and do their best to produce and distribute ginseng products.