• 대한지역사회영양학회지
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• 제22권3호
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• pp.207-217
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• 2017

Objectives: The aim of this study was to examine self-management status, nutritional knowledge, barrier factors in dietary management and needs of nutritional management program for women with Gestational Diabetes Mellitus (GDM). Methods: A total of 100 women with GDM were recruited from secondary and tertiary hospitals in Seoul. The questionnaire composed of general characteristics, status of self-management, dietary habits, nutrition knowledge, barrier factors in dietary management, needs for nutrition information contents and nutritional management programs. Data were collected by a self-administered questionnaire. All data were statistically analyzed using student's t-test and chi-square test using SAS 9.3. Results: About 35% of the subjects reported that they practiced medical nutrition and exercise therapy for GDM control. The main sources of nutrition information were 'internet (50.0%)' and 'expert advice (45.0%)'. More than 70% of the subjects experienced nutrition education. The mean score of nutrition knowledge was 7.5 point out of 10, and only about half of the subjects were reported to be correctly aware of some questions such as 'the cause of ketosis', 'the goal of nutrition management for GDM', 'the importance of sugar restriction on breakfast'. The major obstructive factors in dietary management were 'eating more than planned when dining out', 'finding the appropriate menu when dining out'. The preferred nutrition information contents in developing management program were 'nutritional information of food', 'recommended food by major nutrients', 'the relationship between blood glucose and food', 'tips on menu selection at eating out'. The subjects reported that they need management program such as 'example of menu by calorie prescription', 'recommended weight gain guide', 'meal recording and dietary assessment', 'expert recommendation', 'sharing know-how'. Conclusions: Based on the results of this study, it is necessary to develop a program that provide personalized information by identifying the individual characteristics of the subjects and expert feedback function through various information and nutrition information contents that can be used in real life.

• 대한핵의학회지
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• 제9권2호
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• pp.39-46
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• 1975

It is the purpose of this paper to design the most preferable method of erythropoietin bioassay in Korea. Bioassay utilizing polycythemic mice are currently in general use for the indirect determination of erythropoietin. Assay animals are usually prepared either by transfusion or by exposure to reduced oxygen tension in specially constructed chamber. We prepared the polycythemic, mice by the specially constructed hypobaric chamber. We observed weights and hematocrits of the mice in the hypobaric chamber, then hematocrits and 72 hours $^{59}Fe$ red cell upatke ratio of the polycythemic mice induced by hypoxia after removal from the hypobaric chamber. We designed the method of erythropoietin bioassay according to the results obtained by above experiments. Then we measured the 72 hours $^{59}Fe$ red cell uptake ratio of the polycythemic mice with normal saline, normal plasma and anemic plasma according to the method we designed. The results are followed: 1. The hematocrits of the mice in hypobaric chamber increased to 74% in 14 days It is preferable to maintain the pressure of the chamber to 400mmHg for first 4 days then 300mmHg for last 10 days to reduce the death rate and time consuming in hypobaric chamber. 2. After removal from the hypobaric chamber, the 72 hours $^{59}Fe$ red cell uptake ratio decreased rapidly and maintained the lowest level from the fourth day to tenth day. 3. We design the method of erythropoietin bioassay according to the results of above experiment and to the half life of erythropoietin. 4. The Korean product S9Fe is mixture of $^{59}Fe\;and\;^{55}Fe$. And the $^{59}Fe$ red cell uptake ratio in normal mice was far less with Korean product $^{59}Fe$ than with pure $^{59}Fe$ of foreign product. So it is desirable to use pure $^{59}Fe$ in this method of erythropoietin bioassay. 5. Considering the cost, the technique, the time consuming and the sensitivity it is the most preferable method of erythropoietin bioassay in Korea using hypobaric chamber to induce the polycythemia.

• 한국식품과학회지
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• 제14권3호
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• pp.244-249
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• 1982

낙과(落果)나 미숙과(未熟果)를 사용(使用)한 저농도주정함유(低濃度酒精含有)$(6{\sim}9%)$사과주의 양조(釀造)에 있어서 과도한 산(酸)을 감소(減少)시키기 위하여 사과산분해효모(分解酵母)인 Schizosaccharomyces japonicus var. japonicus St-3의 이용성(利用性)을 검토(檢討)하였다. 원료(原料)사과를 착즙(窄汁)하고 9% 사과주의 경우는 base wine 발효전(醱酵前)에 18%의 당농도(糖濃度)가 되게 보당(補糖)하였다. base wine은 알코올발효(醱酵)(Saccharomyces sp. R-11 이용(利用)) 후기(後期)에 malo-alcohol발효(醱酵)(Schizosaccharomyces japonicus var. japonicus St-3 이용(利用))를 유도(誘導)하는 것이 감산(減酸) 및 품질면(品質面)에서 가장 양호(良好)하였다. 후발효(後醱酵)는 Saccharomyces sp R-11을 이용(利用)하여 저온(低溫)$(7{\sim}8^{\circ}C)$에서 약 130일(日) 동안 지속되었다. 후발효전(後醱酵前)에 $CO_2$가스생성(生成)을 위하여 당(糖) 2%를 첨가(添加)하였으며 포지력(泡持力)을 증강(增强)시키기 위하여 5%의 맥아즙(麥芽汁) 및 hop 즙(汁)이 각각 첨가(添加)되었다. 후발효(後醱酵) 종료후(終了後)에 병내(甁內) $CO_2$ 압력(壓力) $2.3{\sim}2.5kg/cm^2C$을 유지함으로써 저장성(貯藏性)이 부여되었으며 저농도(低濃度) 주정함유(酒精含有)사과주 양조(釀造)가 가능(可能)했다.

• 한국환경농학회지
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• 제9권1호
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• pp.15-22
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• 1990

Chlorothalonil의 참깨 재배중 살포횟수 및 처리시기에 따른 잔류정도와 토양에 처리한 후의 경시적 변화를 관찰하여 다음과 같은 결과를 얻었다. 1. Chlorothalonil의 잔류분석 회수율은 참깨시료를 사용하였을 경우 0.1ppm과 1.0ppm에서 각각 평균 91.2%, 89.6%를 보였고 검출한계는 0.01ppm이었다. 토양시료에서는 0.05ppm과 0.5ppm의 처리에 대한 회수율이 각각 평균 92.8%와 95.4%이었으며 검출 한계는 0.002ppm이었다. 2. Chlorothalonil의 참깨에서의 잔류분석은 Mills등의 방법에서 가장 우수한 성적을 얻을수 있었고 그 결과 수확 3일전 6회 처리는 0.06ppm, 수확 7일전 5회처리는 0.05ppm이 잔류되어 있음을 확인했으며 chlorothalonil의 참깨에 대한 안전사용은 수확 7일 전한, 5회 이내의 사용으로 추천할수 있었다. 3. Chlorothalonil의 토양에서의 잔류분석 결과 처리 0일째 평균 12.95ppm이던 잔류량이 처리후 10일째는 3.28ppm으로 감소되었고 100일이 경과한 후에는 0.03ppm만이 잔류되어 있었으며 곡선회귀식에 의한 chlorothalonil의 토양중 반감기는 8.9일 이었다.

• 한국도로학회논문집
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• 제13권2호
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• pp.31-39
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• 2011

국내 고속도로의 60% 이상이 시멘트 콘크리트 포장으로 건설되었으며, 그 중 공용년수가 20년이 넘어선 구간이 절반 이상에 달하고 있다. 노후화된 시멘트 콘크리트 포장의 보수 보강은 국내의 교통여건상 우회도로가 준비되기 어렵기 때문에 조기 교통개방이 요구되며 현재까지는 주로 아스팔트 덧씌우기가 사용되고 있다. 반면에 아스팔트 덧씌우기 포장은 조기 파손으로 인해 많은 유지보수비용이 지출됨과 동시에 도로사용자의 불편을 초래하고 있다. 최근 들어 노후화 된 시멘트 콘크리트 포장의 효율적인 보강공법으로 접착식 콘크리트 덧씌우기 공법에 대해서 적용을 시도하고 있다. 따라서 아스팔트 덧씌우기 포장과 접착식 콘크리트 덧씌우기 포장의 합리적인 선택에 대한 비교 분석을 위해 포장의 연도별 파손상태에 대한 다양한 data가 필요하다. 하지만, 국내에서는 아스팔트 덧씌우기 포장과 접착식 콘크리트 덧씌우기 포장의 공용 중 파손상태에 대한 자료가 체계적으로 구축되어있지 않다. 본 연구에서는 아스팔트 덧씌우기와 접착식 콘크리트 덧씌우기 공법이 적용된 구간의 파손에 대해 충분한 자료를 구축하고 있는 미국의 LTPP Data를 이용하여, 공용성에 대해서 평가하였다. 단, 아스팔트 덧씌우기와 접착식 콘크리트 덧씌우기 공법은 파손형태가 서로 상이함으로써, 상대적인 비교를 위해 각각의 구간에 대해 포장상태지수(PCI, Pavement Condition Index)를 Database화 하였으며, 아스팔트 덧씌우기 구간과 접착식 콘크리트 덧씌우기 구간의 수명에 대해서 비교 분석을 수행하였다.

• 응용통계연구
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• 제22권4호
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• pp.771-780
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• 2009

백내장 질환은 노령인구가 증가하고 있는 시점에서 사회, 경제적으로 심각한 문제로 부각되고 있는 질병으로 조기 진단이 이루어진다면 발병률을 크게 줄일 수 있는 질병이다. 본 연구에서는 백내장을 조기 진단하기 위한 예측 모형을 구축하고자 1994년부터 2001년까지 연세대학병원에서 2회 이상 건강검진을 받고 의사진단을 통해 백내장 여부를 확인할 수 있는 30세 이상 남 녀 3,237명에 대한 건강검진 수검 자료를 활용하여 백내장 발생 위험 예측모형을 개발하였다. 모형개발에는 데이터마이닝 기법인 Random Forests를 사용하였고, 기존의 로지스틱 회귀분석, 판별분석, 의사결정나무 모형(Decision tree), 나이브베이즈(Naive Bayes), 앙상블 모형인 배깅(Bagging)과 아킹(Arcing)을 이용하여 그 성능을 비교 분석하였다. Random Forests를 통해 개발한 백내장 발생 예측모형은 정확도가 67.16%, 민감도가 72.28%였고, 주요 영향요인은 연령, 혈당, 백혈구수치(WBC), 혈소판수치(platelet), 중성지질(triglyceride), BMI였다. 이 결과는 의사의 안과검진 정보 없이 건강검진 수검 자료만으로 백내장 질환 유 무에 관한 정보를 70% 정도 예측할 수 있음을 보여주는 것으로, 백내장의 조기 진단에 많은 기여를 할 것으로 판단된다.

• Biomolecules & Therapeutics
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• 제18권4호
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• pp.469-476
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• 2010

This study was to investigate the effect of apigenin on the bioavailability of paclitaxel after oral and intravenous administration in rats. The effect of apigenin on P-glycoprotein (P-gp), cytochrome P450 (CYP)3A4 activity was evaluated. The pharmacokinetic parameters of paclitaxel were determined in rats after oral (40 mg/kg) or intravenous (5 mg/kg) administration of paclitaxel with apigenin (0.4, 2 and 8 mg/kg) to rats. Apigenin inhibited CYP3A4 activity with 50% inhibition concentration ($IC_{50}$) of 1.8 ${\mu}M$. In addition, apigenin significantly inhibited P-gp activity. Compared to the control group, apigenin significantly increased the area under the plasma concentration-time curve (AUC, p<0.05 by 2 mg/kg, 59.0% higher; p<0.01 by 8 mg/kg, 87% higher) of oral paclitaxel. Apigenin also significantly (p<0.05 by 2 mg/kg, 37.2% higher; p<0.01 by 8 mg/kg, 59.3% higher) increased the peak plasma concentration ($C_{max}$) of oral paclitaxel. Apigenin significantly increased the terminal half-life ($t_{1/2}$, p<0.05 by 8 mg/kg, 34.5%) of oral paclitaxel. Consequently, the absolute bioavailability (A.B.) of paclitaxel was significantly (p<0.05 by 2 mg/kg, p<0.01 by 8 mg/kg) increased by apigenin compared to that in the control group, and the relative bioavailability (R.B.) of oral paclitaxel was increased by 1.14- to 1.87-fold. The pharmacokinetics of intravenous paclitaxel were not affected by the concurrent use of apigenin in contrast to the oral administration of paclitaxel. Accordingly, the enhanced oral bioavailability by apigenin may be mainly due to increased intestinal absorption caused via P-gp inhibition by apigenin rather than to reduced renal and hepatic elimination of paclitaxel. The increase in the oral bioavailability might be mainly attributed to enhanced absorption in the gastrointestinal tract via the inhibition of P-gp and reduced first-pass metabolism of paclitaxel via the inhibition of the CYP3A subfamily in the small intestine and/or in the liver by apigenin. It appears that the development of oral paclitaxel preparations as a combination therapy is possible, which will be more convenient than the i.v. dosage form.

• Nutrition Research and Practice
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• 제14권2호
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• pp.102-108
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• 2020

BACKGROUND/OBJECTIVES: In Oriental medicine, certain foods may be beneficial or detrimental based on an individual's constitution; however, the scientific basis for this theory is insufficient. The purpose of this study was to investigate the effect of body constitution, based on the Sasang type of Korean traditional medical classification system, on the bioavailability of soy isoflavones of Cheonggukjang, a quick-fermented soybean paste. SUBJECTS/METHODS: A pilot study was conducted on 48 healthy Korean men to evaluate the bioavailability of isoflavone after ingestion of food based on constitution types classified by the Sasang typology. The participants were classified into the Taeeumin (TE; n = 15), Soyangin (SY; n = 15), and Soeumin (SE; n = 18) groups. Each participant ingested 50 g of Cheonggukjang per 60 kg body weight. Thereafter, blood was collected, and the soy isoflavone metabolites were analyzed by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. Ntrikinetic analysis of individual isoflavone-derived metabolites was performed. RESULTS: Our nutrikinetic analysis identified 21 metabolites derived from isoflavones in the blood samples from 48 healthy Korean men (age range, 21-29 years). Significant differences were observed in the time to maximum concentration (T_max) and elimination half-life (t_1/2) for nine metabolites among the three groups. The T_max and t_1/2 of the nine metabolites were higher in the SE group than in the other groups. Moreover, the absorption rates, as determined by the area under the plasma-level curve (AUC) values of intact isoflavone, were 5.3 and 9.4 times higher in the TE group than in the SY and SE groups, respectively. Additionally, the highest AUC values for phase I and II metabolites were observed in the TE group. CONCLUSIONS: These findings indicate that isoflavone bioavailability, following Cheonggukjang insgestion, is high in individuals with the TE constitution, and relatively lower in those with the SE and SY constitutions.

• 생물환경조절학회지
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• 제5권2호
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• pp.174-186
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• 1996

This study was carried out in order to understand the plan, design, constructing and actual condition of management of modernized horticultural facilities in Kyungpook Province which had been constructed from 1992 to 1995 funded by Government support. The aim of this study is to provide reference data for success of the forth project. It was performed by making up a question about driving of project and management condition of equipment after constructing. The results obtained from this study are as follows: 1. 73.5% of facilities horticulture farmhouse recognized that the prospect of greenhouse is bright, but 92.5% of the farmhouse also recognised that they need technical consultation on protected horticulture farming. Therefore, technical educations would must be enhanced about foundation of greenhouse and cultivation technique. 2. The holding times of explanatory meetings, cause of understanding to farmhouse, were one or two times in greenhouse construction, and 62.5% of the farmhouse expressed the insufficiency at the explanation and educational data. For this reason, it was judged that the construction contract had been delayed more than 5 months in 49.3% of the farmhouse after the decision of project budget. 3. In constructing after a contract, the rates of construction delay is 53.4% and defect occurrence is 41.1%. The biggest reasons of construction delay was insufficiency of worker and materials supply. Each percentage is 29.1%. And the reason of defect occurrence is badness of machinery equipment(62.9% ). 4. In management of greenhouse, a pipe-constructed plastic film greenhouse changes plastic film every one and three years because of sticking dust on plastic film. It was needed to about in cleaning technique of coverings. Because that used 3-5 years only half of the expected life span. 5. The order of broken rating in the subsidiary equipment is like this lollop ventilator (42.8%), a general control system(33.3%) especially, in the case of a general control system, the rate of all family can control is 52.7%. so, it is time to develop easy control equipment which every one could use as soon as possible. 6. When choose heat generator as decide capacity, the most priority is the mount of heat generator the percent is 45.5% heat generator and as decide model, the private purchase's percent is 77.3%. It is higher than a public bidding heat generator the percent is 22.7% heat generator when it compare with a public bidding. In the case of $CO_2$ generator, using rate is only 19.0%. The using rate is very low, so it needs education how to use depends on the way of the subsidiary equipment. 7. In the case of seedlings, it is asked to use factory-processed seedling effectively. because it's difficult to get security of labors(58.8%), hoped crops (55.9%) access same crops(29.4%) much more and changing of crops depends on market situation. that is the main reason the lack of knowhow.

• 한국식품과학회지
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• 제36권3호
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• pp.440-447
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• 2004

본 연구에서는 유전자 재조합 기술에 의해 제조된 ${\beta}-carotene$ 15,15'-dioxygenase의 반응특성 및 효소 kinetics를 규명하였다. ${\beta}-carotene$ 15,15'-dioxygenase 효소반응을 위한 최적 온도 및 pH를 측정한 결과, 최적 온도는 $40^{\circ}C$로 판명되었으며 최적 pH 는 9.0이었다. 저장 pH 6.0-9.0 범위에서 안정하였으며, pH 11에서도 80% 이상의 활성을 보이는 호알칼리성 효소임을 확인하였다. 온도 저장성을 확인한 결과, $35^{\circ}C$에서의 효소활성 반감기가 40분으로서 열에 민감한 것으로 판단되었다. 한편, ferrous ion-chelating agent와 sulfhydryl-binding agent를 사용하여 ${\beta}-carotene$ 15,15'-dioxygenase에 미치는 영향을 살펴 보았다. Ferrousion-chelating agent인 ${\alpha},{\alpha}'-dipyridyl$과 1,10-phenanthroline은 $1{\times}10^{-4}$ M에서 최소 저해농도를 형성하였으며, sulfhydryl-binding agent인 iodoacetamide와 PCMB는 $1{\times}10^{-3}$ M에서 N-ethylmaleimide은 $1{\times}10^{-4}$} M에서 최소저해농도를 형성함을 확인할 수 있었다. 본 연구에서의 효소반응은 Michaelis-Menten 곡선을 따름을 확인하였으며, Hanes-Woolf 작도법에 따른 결과, ${\beta}-carotene$ 15,15'-dioxygenase 효소의 $K_{m}$ 및 $V_{max}$ 값은 각각 $3.39{\times}10^{-6}$ 및 1.2 pmol/mg protein/min인 것으로 산출되었다.