• Journal of Mechanical Science and Technology
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• v.15 no.12
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• pp.1741-1749
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• 2001

Recent studies of the structure of wall turbulence have lead to the development of a conceptual model that validates and integrates many elements of previous models into a relatively simple picture based on self-assembling packets of hairpin vortex eddies. By continual spawning new hairpins the packets grow longer in the streamwise direction, and by mutual induction between adjacent hairpins the hairpins are strained so that they grow taller and wider as they age. The result is a characteristic growth angle in the streamwise-wall normal plane. The spanwise growth of individual packets implies that packets must either merge or pass through each other when they come into contact. Direct numerical simulations of the growth and interaction of spanwise adjacent hairpins shows that they merge by the vortex connection mechanism originally proposed by Wark and Nagib (199). In this mechanism the quasi-streamwise legs of two hairpins annihilate each other, by virtue of having opposite vorticity, leaving a new hairpin of approximately double the width of the individuals. PIV measurements in planes parallel to the wall support this picture. DNS of multiple hairpins shows how the spanwise scale doubles when the hairpins form an array.

• Journal of the Korean Society of Clothing and Textiles
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• v.28 no.11
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• pp.1404-1414
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• 2004

This study examines how the stereotype in a society toward the roles of teachers and students affects their hair styles and ornaments and defines it through the cultural tag, 'like'. A survey of 362 male and female students in colleges located in Seoul and Gyeonggi areas was conducted. The data analysis was done by SPSS 10.0 and the difference of sample means between the groups in addition to basic statistics was evaluated. The t-test was made to test statistically significant difference between the groups. The results of the analysis are summarized as follows: First, the hair styles, shoes and ornaments that are recognized as appropriate for the roles of male students are short cut and black hair, sneakers and necktie. In that case of female students, medium and long hair style, black and brown hair, Loafer, hairpin and hairband are recognized properly. The hair styles, shoes and ornaments that are recognized as appropriate for the roles of male teachers are short cut and black hair, oxfords and necktie. In that case of female teachers, cut, medium, long, permanent and up style, 1)lack and brown hair, pumps, necklace, ring, earring, bracelet, hairpin, hairband, scarf and muffler are recognized appropriately. Second, the stereotype of male students and teachers's roles was more traditional, normative, and limited than that of female students and teachers's. The role of female teachers showed more generous than that of male students, female students, and male teachers.

• Bioinformatics and Biosystems
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• v.2 no.1
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• pp.17-23
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• 2007

Shot interfering RNA (siRNA) can be used to silence specific gene expression and have many potential therapeutic applications. However, how to design an effective siRNA is still not clear. Highly effective siRNA has sequence-specific properties which are low G/C content, low internal stability at the sense strand 3'-terminus, sense strand base bias(position 1 is G/C, position 19 is /AU). Recently, mRNA secondary structure playsan important role in RNAi. Target site of siRNA in high-ordered structure (i.e hairpin loop, multi loop) or base pair of many hydrogen bonds dramatically reduce function of siRNA mediated gene silencing. Possible off-target effects of siRNA is detecting from BLAST search.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07a
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• pp.219-222
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• 2003

In this paper, we designed a Ku-band BPF(Band Pass Filter) by microstrip line that most usually used a microwave device design and fabrication. Here a substrate of designed BPF were silicon substrate(${\varepsilon}_r=11.8$), and metal line was copper and silver/copper structure. And a configration of BPF was used hairpin pattern. A center frequency of designed BPF was 10GHz and their FBW(Fractional Band Width) was 20%(2GHz). It presented simulated results obtained for a 10GHz filter which yields an insertion loss of 0.1dB that ripple value related chebyshev reponse. Finallt we tried to make that a 30dB attenuation frequency was 20% of center frequency.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.142-142
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• 2011

Microwave diagnostics method for plasma science and engineering is vigorous research area for its good characteristics such as high sensitivity, reliability, and broad measurement spectrum from low density plasma to high density. We investigate mechanism of microwave probes (hairpin, impedance and absorbtionf probe) and apply it for interpretation of full transmitted spectrum of cutoff probe. Mechanism of the spectrum having same key roles of I-V curve of Langmuir probe is not exactly revealed yet in spite of its importance. This study elucidates physics behind it using a circuit model and E/M wave simulation. Circuit model reveals exact cut-off peak frequency taking account of a collision frequency and a plasma frequency and it enable precise diagnostics of plasma densty from low pressure to high pressre. Cut-off like peaks have been obstacle for choosing cut-off peak is analyzed by E/M simulation and one of cutoff like peaks made by probe holder used for acquire plasma density with cutoff peak applying the hairpin relation. Furthermore, phase difference method for plasma density is conducted. This method uses a single microwave frequency source and it is low-priced.

• Molecules and Cells
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• v.38 no.11
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• pp.959-965
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• 2015

Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNA-expression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.22 no.11
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• pp.767-772
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• 2010

The incompressible viscous flow past a sphere under forced oscillation is numerically investigated at a Reynolds number of 300. The immersed boundary method is used to handle the sphere oscillating vertically to the streamwise direction. There are two important variables to characterize the oscillating state of a sphere. One is an oscillating amplitude normalized by the sphere diameter is set as a fixed number of 0.2. Another is the frequency ratio which is defined by $f_e/f_o$, where fe and fo are the excited frequency and the natural frequency of vortex shedding for the stationary sphere. In this study, three different frequency ratios of 0.8, 1.0 and 1.2 are considered. The results show a periodic flow with hairpin vortices shedding from upper and lower positions as well as vortical legs obliquely extended by oscillating motion of sphere. The enveloping vortical structure experience rupture twice in one period of oscillation. As the frequency of oscillation is increased, the vortical legs are getting shorter and eventually the hairpin vortices are much closer to the adjacent one.

• ETRI Journal
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• v.35 no.2
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• pp.344-347
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• 2013

This letter presents a compact dual-mode tri-band bandpass filter by using a short-circuited stub-loaded stepped-impedance resonator (SIR) and a short-circuited stub-loaded uniform impedance resonator. Also, a hairpin SIR geometry is introduced to miniature the size of this filter while maintaining excellent performance. The use of a short-circuited stub at the central point of the hairpin SIR can generate two resonant modes in two passbands. Its equivalent circuit structure is analyzed by using the even-odd mode theory. For demonstration purposes, a tri-band filter for the applications of the Global Positioning System at 1.57 GHz, Worldwide Interoperability for Microwave Access at 3.5 GHz, and wireless local area networks at 5.2 GHz is designed, fabricated, and measured.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.5
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• pp.1175-1188
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• 1988

Linear estimation in isotropic turbulence is examined to approximate conditional averages in the form of fluctuating velocity fields conditioned on local velocity. The conditional flow fields and their associated vorticity field are computer using experimental data [Van Atta and Chen] and energy spectrum model [Driscoll and Kennedy]. It appears that ring vorticies could be the dominant structure. Due to the extremely large vorticity in the viscous region of a conditional ring vortex, the energy spectrum model can be used appropriately by changing the Reynolds number. The hairpin vortex could be detected by combining vorticies in isotropic field with an anisotropic orientation imbedded in uniform mean shear flow and this is consistent with other studies [Kim and Moin].

• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1544-1549
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• 2024

This study presents a fluorescent mechanism for two-step amplification by combining two widely used techniques, exponential amplification reaction (EXPAR) and catalytic hairpin assembly (CHA). Pseudomonas aeruginosa (P. aeruginosa) engaged in competition with the complementary DNA in order to attach to the aptamer that had been fixed on the magnetic beads. The unbound complementary strand in the liquid above was utilized as a trigger sequence to initiate the protective-EXPAR (p-EXPAR) process, resulting in the generation of a substantial quantity of short single-stranded DNA (ssDNA). The amplified ssDNA can initiate the second CHA amplification process, resulting in the generation of many double-stranded DNA (dsDNA) products. The CHA reaction was initiated by the target/trigger DNA, resulting in the release of G-quadruplex sequences. These sequences have the ability to bond with the fluorescent amyloid dye thioflavin T (ThT), generating fluorescence signals. The method employed in this study demonstrated a detection limit of 16 CFU/ml and exhibited a strong linear correlation within the concentration range of 50 CFU/ml to 10⁵ CFU/ml. This method of signal amplification has been effectively utilized to create a fluorescent sensing platform without the need for labels, enabling the detection of P. aeruginosa with high sensitivity.