• Title/Summary/Keyword: Hair Analysis

Search Result 726, Processing Time 0.025 seconds

Expression of Coat Color Associated Genes in Korean Brindle Cattle by Microarray Analysis

  • Lee, Hae-Lee;Park, Jae-Hee;Kim, Jong Gug
    • Journal of Embryo Transfer
    • /
    • v.30 no.2
    • /
    • pp.99-107
    • /
    • 2015
  • The aim of the present study was to identify coat color associated genes that are differentially expressed in mature Korean brindle cattle (KBC) with different coat colors and in Hanwoo cows. KBC calves, before and after coat color appearance, were included. Total cellular RNA was isolated from the tail hair cells and used for microarray. The number of expressed coat color associated genes/probes was 5813 in mature KBC and Hanwoo cows. Among the expressed coat color associated genes/probes, 167 genes were the coat color associated genes listed in the Gene card database and 125 genes were the pigment and melanocyte genes listed in the Gene ontology_bovine database. There were 23 genes/probes commonly listed in both databases and their expressions were further studied. Out of the 23 genes/probes, MLPH, PMEL, TYR and TYRP1 genes were expressed at least two fold higher (p<0.01) levels in KBC with brindle color than either Hanwoo or KBC with brown color. TYRP1 expression was 22.96 or 19.89 fold higher (p<0.01) in KBC with brindle color than either Hanwoo or KBC with brown color, respectively, which was the biggest fold difference. The hierarchical clustering analysis indicated that MLPH, PMEL, TYR and TYRP1 were the highly expressed genes in mature cattle. There were only a few genes differentially expressed after coat color appearance in KBC calves. Studies on the regulation and mechanism of gene expression of highly expressed genes would be next steps to better understand coat color determination and to improve brindle coat color appearance in KBC.

Effects of Electrical Stimulation on Wound Healing and Skin Mast Cells in Streptozotocin-Induced Diabetic Rats (스트렙토조토신 유도 당뇨 흰쥐에서 전기자극이 상처치유와 피부 비만세포에 미치는 영향)

  • Jekal, Seung-Joo;Lee, Kyung-Sun;Chung, Ok-Bong;Lee, Jae-Hyoung
    • Korean Journal of Clinical Laboratory Science
    • /
    • v.40 no.2
    • /
    • pp.118-128
    • /
    • 2008
  • The aim of this study was to investigate the effect of electrical stimulation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Thirty male Sprague-Dawley rats were divided into three groups : incision (control), diabetes+incision (diabetes) and diabetes + incision + electrical stimulation (D/ES). Diabetes was induced in rats by streptozotocin (STZ) injection (60 mg/kg, one time) and 20 mm length incision wounds were created on the back after shaving hair. The electrical stimulation rats were treated with a current intensity of 30~50 V at 120 pps and $140{\mu}s$ for 10 days from 3 days after STZ injection. The lesion and adjacent skin tissues were fixed with 10% buffered formalin, embedded with paraffin. For wound healing analysis, hematoxylin-eosin (HE) and picrosirius red staining were performed. Mast cells (MC) were stained with toluidine blue (pH 0.5) and quantified at ${\times}200$ using a light microscope. The density of keratinocyte proliferation and microvessels in skin tissues were analyzed using a computerized image analysis system on sections immunostained with proliferative cell nuclear antigen (PCNA) and ${\alpha}$-smooth muscle actin (${\alpha}$-SMA), respectively. The results showed that the wound healing rate, collagen density and neoepidermis thickness, density of PCNA-positive cells and density of ${\alpha}$-SMA-positive vessels were significantly higher in D/ES rats than in diabetic rats. The density of MCs and degranulated MCs in D/ES rats were also significantly higher than those in diabetic rats. These findings suggest that the electrical stimulation may promote the tissue repair process by accelerating collagen production, keratinocyte proliferation and angiogenesis in the diabetic rats, and MCs are required for wound healing of skin in rats.

  • PDF

Clinical Analysis of Varicose Veins in the Lower Extremities - an analysis of 227 operative cases - (하지정맥류의 임상양상 - 227 수술예의 분석 -)

  • 최수승
    • Journal of Chest Surgery
    • /
    • v.32 no.11
    • /
    • pp.1036-1041
    • /
    • 1999
  • Background: To know the clinical aspects of varicose vein and the effectiveness of operative treatment, the patients who had been operated during the five and a half years were reviewed. Material and Method: From September 1993 to February 1999, 227 patients with varicose vein were operated upon. These patients were reviewed with history taking, physical examination, laboratory tests, operation and follow up visits at an out patient department. Result: The ages of the patients were from 20 to 69 years, mean 40.2 years. varicose veins. Occupations of the patients include housewives, store or factory owners, cooks, waiter or waitresses in a restaurant, salary men(women), teachers, hair stylists, professional athletes, in sequence, most of them require standing all day long. The 126 women had experienced child birth, among them 116 patients delivered more than 2 babies. All patients had protruding leg veins as the symptom. Most of them(61%) had pain. Other additional symptoms were heaviness, heatness, tingling, cramp, fatigue, etc. 68.3% of the patients had the symptoms for more than 10 years. Anatomical location of varicose vein was in long saphenous vein in 157 patients, in small saphenous vein in 27, and in other regions(combined or perforating vein) in 43 patients. There was no operative mortality. There were 2 patients who underwent reoperation due to recurrence during this study period. In one patient, it recurred in the distal perforating vein in small saphenous vein. The other patient had recurrence at the saphenofemoral junction in groin. The patients are doing well after the reoperation, but a long term observation is needed. Conclusion: Clinical aspects of varicose vein were reviewed. For the patients with varicose vein who had saphenofemoral regurgitation, operative treatment seemed to bea safe and effective modality of treatment.

  • PDF

A Study on the Subjective Evaluation and Physical Properties of Natural/Artificial Rabbit Hairs (천연 인조 토끼털의 주관적 평가 및 물리적 성질에 관한 연구)

  • Lee, Seon Ah;Kim, Jongjun
    • Journal of Fashion Business
    • /
    • v.21 no.4
    • /
    • pp.144-158
    • /
    • 2017
  • Fur garment has long been the conventional symbol for luxury, or conspicuous consumption. However, as fashion items began to diversify as part of overall fashion trend, fur items are now more about individual taste and style than just lavishness. Synthetic fur is especially emerging as a new promising fashion material, with a touch almost like natural fur at an affordable price. Along with the emergence of 'Vegan Fashion' trend, synthetic fur is establishing itself as a popular fashion textile. This study is an attempt to investigate subjective evaluation and physical properties of natural and synthetic furs, whose results will further serve as basic data in developing synthetic fur materials. Sensory and emotional evaluations are carried out on natural and artificial furs. For analysis, factors such as weight, thickness, air permeability, gloss and compressibility were surveyed to observe how they influence the physical properties. According to the subjective evaluation, natural and artificial fur samples do not differ in conspicuous ways in appearance. Experiments on physical properties, specifically warm/cool touch experiment, show that natural fur has a slightly higher warm sensation than artificial fur. Luster analysis by using a microscope revealed that there are subtle qualitative differences between natural and artificial fur. During the subjective evaluation, subjects found it hard to state distinct quantitative differences in luster. A survey as a means of assessing qualitative differences in gloss seems to be necessary to complement the evaluation. Results from this study will potentially serve as resources for diversification of fashion product designs using synthetic fur.

Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus)

  • Wang, X.J.;Shi, J.J.;Yang, J.F.;Liang, Y.;Wang, Y.F.;Wu, M.L.;Li, S.Y.;Guo, X.D.;Wang, Z.G.;Liu, D.J.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.25 no.5
    • /
    • pp.606-612
    • /
    • 2012
  • Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

An analysis on the Factors of the Consumption Expenditure on K-beauty Service Industry (K-beauty 서비스산업 소비지출의 영향요인 분석)

  • Chin, Chung-Hwa;Yuk, Hyun-Jeong
    • Journal of the Korea Convergence Society
    • /
    • v.11 no.7
    • /
    • pp.351-359
    • /
    • 2020
  • This study aims to propose for the K-beauty industry by analyzing the consumption situation. The data used the household trend survey and GDP from 06' to 16' provided by the National Statistical Office. The analysis results of the influencing factors of expenditure on the K-beauty service industry per household resulted in the increase of expenditure on hair and beauty services and their goods when ordinary income decreased, it was possible to understand the similarities between the change of Engel's coefficient. It was difference in the proportion of consumption of beauty services account by income quintiles.The increase of the GNI had an effect on that of the consumption of K-beauty. The above results show that beauty service industry is relevant to the items of households' income and expenditure. Plans are needed to take into account national economic trends and consumption patterns.

Keratinocyte Proliferation in Aged Rat Skin by High Voltage Pulsed Current Stimulation

  • Lee Jae-Hyoung;Lee Jong-Sook;Kil Eyn-Young
    • Biomedical Science Letters
    • /
    • v.10 no.4
    • /
    • pp.361-366
    • /
    • 2004
  • The purpose of this study was to determine the effect of high voltage pulsed current (HVPC) stimulation on proliferative activities of basal keratinocytes by measured nucleolar organizer region (NOR) expression and thickness of spinous layer in aged rat skin. Fifty-one weeks old twelve male Sprague-Dawley rats (300∼350g) were divided into control and HVPC stimulation groups. Each animal's hair on the back were removed. The HVPC stimulation group received an negative monophasic twin peak pulsed current stimulation with 50 V, while the control group was given the same treatment without electricity. The rats were sacrificed after 3 weeks. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with hematoxyline-eosin and silver nitrate. The thickness of basal to granular layer of the epidennis were measured using a light microscope and computerized image analysis system. The number of argyrophilic nucleolar organizer region (AgNOR) were counted using a light microscope and computerized image analysis system and calculated as the mean number of AgNOR per nucleus in the basal keratinocyte. By using a Student's t-test, an increase in the thickness of basal-spinous layer (P<0.001) of epidermis can be observed in HVPC stimulation rats as compared with the control rats, whereas the thickness of the granular layer is not affected. A Student's t-test showed a significantly higher mean NOR number per nucleus of the basal keratinocyte in the HVPC stimulation rats than control rats (P<0.001). There was significantly positive correlation between the NOR number and the thickness of basal-spinous layer (r=0.80, P<0.05). These results suggest that the HVPC stimulation may increase the thickness of spinous layer in the epidennis due to increased proliferative activities of basal keratinocytes in epidennis in aged rat skin.

  • PDF

Analysis of the Relational Structure among Service Failure-related Variables after Moderation of Fairness - Focusing on fairness-related - (공정성 조절효과에 따른 서비스 실패 관련 변인들 간의 관계구조분석 - 공정성 조절효과를 중심으로 -)

  • Kim, Seong-Ah;Yoo, Tai-Soon
    • Journal of the Korean Society of Costume
    • /
    • v.64 no.3
    • /
    • pp.13-31
    • /
    • 2014
  • This study attempts to analyze relational structures among service failure-related variables after the moderation of fairness in the beauty service industry with the following purposes: First, it aims to review and investigate service failure & service recovery strategies, non-switching intentions after recovery, revisit intention, the intent to provide word-of-mouth recommendations and previous studies on service failure and recovery in the beauty service industry. Second, it targets the analysis of the role of fairness as a variable that moderates relations between service recovery strategies and post-recovery satisfaction in the beauty service industry. For this, the following research method was used: This study has investigated the effect of service failure and its recovery strategies (behavioral recovery strategy, psychological recovery strategy, monetary recovery strategy) on customer satisfaction for beauty service users and used the Structural Equation Model (SEM) to further analyze and verify the effect of the satisfaction on post-satisfaction behavior (non-switching intention, repurchase intention and the intent to provide word-of-mouth recommendations). The SEM was divided into a measurement model and structural model to determine if the model is appropriate and estimate the parameters of the path coefficient. In addition, this study examined to see if fairness (procedural fairness, distributive fairness and interactive fairness) works as a moderating variable while the service recovery efforts affect customer satisfaction. Then, the role of service recovery strategies, targeted to satisfy the customers who were dissatisfied because of service failure, were investigated. In addition, its effect on post-satisfaction behavior was analyzed from the structural aspect, and the moderating role was examined as well. Then, the role of the service recovery strategy, which can be used to satisfy dissatisfied customers, was examined, and the effect of the satisfaction on customer behavior was analyzed from a structural perspective. In addition, the moderating role of fairness was tested. As a result, this study is significant in that it helps service providers formulate service recovery-related strategies.

Assessment of Traditional Knowledge on Seasonal Customs and Its Characteristics for Practical Use (세시풍속 전통지식기술의 개발가치 평가와 활용방안 분석)

  • Kim, Mi-Heui;Park, Duk-Byeong;Ahn, Yoon-Soo;Jun, Young-Mi
    • The Korean Journal of Community Living Science
    • /
    • v.17 no.4
    • /
    • pp.175-197
    • /
    • 2006
  • This study aims to assess the traditional knowledge on seasonal customs and its characteristics for practical use. The Delphi method and correspondence analysis were hired to collect and analysis the data. Twenty six professionals for the Delphi participated in three-round process. The followings were concluded. The number of items valuing and resourcing for seasonal customs. were 118(40 for folks and ceremonies, 53 for foods. 25 for plays) on Delphi results. For example are Bokjori, Dano fm, Bokjumeoni, Chuseok, Tano festival, New Year Card, washing hair with an iris, Soup with rice cake, Boiled rice with five cereals, Rice and red-bean porridge, Kimchi-making for the winter, Sharing walnut with friends, Game of yut, Playing kite, Play with Hanga etc. Nowadays the ideas of modernized practical use on seasonal customs was to connect with modernized scientific technology and designate commemoration day. The items for connecting with modernized scientific technology were new year card, painting and recording sound with traditional seasonal customs, food areas, cake with seven kind of cereals, play areas, hand wrestling, cockfighting in folks and customs areas. Also the items for designating commemoration days were the Suelbeam and Suelbeam Socks present for the aged people, man cooking day, Korean traditional workers day in folk and ceremony areas.

  • PDF

Comparative Study Between Inaba's Procedure and Modified Inaba's Procedure with Delayed Suture in the Treatment of Osmidrosis Axillae (액취증의 치료에서 Inaba씨 방법과 절개창을 지연 봉합한 Inaba씨 변법의 비교 조사)

  • Lee, Seong Pyo;Suhk, Jeong Hoon;Yang, Wan Suk
    • Archives of Plastic Surgery
    • /
    • v.36 no.6
    • /
    • pp.727-734
    • /
    • 2009
  • Purpose: The Inaba's procedure, the treatment of osmidrosis axillae, has the advantages of low recurrent rate and easy to learn, yet it produces early postoperative discomfort and scar formation by tie - over dressing. The authors modified the Inaba's procedure by using delayed suture of the incision wound and omitting tie - over dressing. The comparative study of Inaba's procedure and its modification was performed to confirm the advantages of modified procedure. Methods: The study contains the retrospective analysis of the medical records of 296 patients with osmidrosis who were treated using the Inaba's procedure from December, 1996 to February, 2007. The study also contains the prospective analysis of 20 patients, from March, 2007 to July, 2008, who were treated by the modified Inaba's procedure with delayed suture of the incision wound and gentle pressure dressing instead of tie - over dressing. The operative results of two groups were compared and verified by Mann - Whitney U test(SPSS 12.0). Results: The incidence of complications was 14.5% in the Inaba's procedure, whereas 6.2% in the modified Inaba's procedure. Both procedures have the same basic surgical procedure in terms of the location of incision site and subdermal shaving of the sweat glands, and therefore similar good results were obtained in the aspect of postoperative axillary odor, recurrent rate and postoperative condition of axillary hair. Certainly, the modified Inaba's procedure had better outcome in each element of PSS(Patient Scar Self-Rating Scale), compared to the Inaba's procedure. In addition, the modified Inaba's procedure showed a statistical significance in dressing - related pain reduction and overall satisfaction. Conclusion: The modified Inaba's procedure had advantages of decreased early postoperative complications such as hematoma, discomfort and pain caused by tie - over dressing, and decreased scar formation. However, the drawback was delayed suture of the incision wound after 48 hours.