• 제목/요약/키워드: HT-29

검색결과 575건 처리시간 0.031초

달맞이꽃 종자 추출물의 생리활성 (Biological Activity of Oenothera Biennis Seed Extracts)

  • 조현동;김두현;김민근;이용석;서권일
    • 생명과학회지
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    • 제28권9호
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    • pp.1048-1055
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    • 2018
  • 본 연구에서는 달맞이꽃 종자 물, 에탄올, 메탄올 및 70% 에탄올 추출물의 총 폴리페놀 및 플라보노이드 함량, 항산화, 신경세포 손상 억제, 항암, 대식세포 면역조절 활성 및 에 대하여 비교하였다. 달맞이꽃 종자 추출물 중 메탄올 추출물에서 각각 31.90 mg GAE/g 및 20.66 mg QE/g의 총 폴리페놀 및 총 플라보노이드 함량을 나타내었으며 나머지 추출물과 비교하여 가장 활성이 높았다. 라디칼 소거능 및 환원력 활성은 달맞이꽃 종자 추출물의 처리에 따라 농도의존적으로 증가하였다. 또한 물, 에탄올 및 메탄올 추출물을 $25-100{\mu}g/ml$의 농도로 전처리 하였을 때 글루탐산에 의해 유도되는 신경세포 손상 효과를 감소시키는 것으로 확인되었다. 암세포의 경우 메탄올 추출물이 간암, 폐암, 유방암 및 흑색종 피부암의 세포 생존율을 효과적으로 감소시켜 낮은 $IC_{50}$ 값을 보였으며 대장암과 전립선암에서는 물 추출물과 에탄올 추출물이 가장 낮은 $IC_{50}$ 값을 나타내었다. 또한, 달맞이꽃 종자 70% 에탄올 추출물은 RAW 264.7 마우스 대식세포의 생존율에 영향을 미치지 않는 농도에서 유의적으로 산화질소(nitric oxide, NO)의 생성을 통해 대식세포의 면역활성을 유도하였다. 따라서, 달맞이꽃 종자 추출물은 풍부한 폴리페놀 함량과 다양한 생리활성 효능을 나타내며 이를 통해 달맞이꽃 종자 추출물을 기능성 식품 원료 및 천연물 의약품으로 이용할 수 있을 것으로 사료된다.

Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권12호
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

하절기 가두리양식 참돔의 절식에 따른 성장과 혈액성상의 변화 (Change of Growth and Blood Characteristics on Starvation of Red Seabream Pagrus major Cultured in Marine Net Cage in Summer)

  • 김원진;원경미;신윤경;이정용
    • 한국어류학회지
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    • 제29권3호
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    • pp.165-175
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    • 2017
  • 본 연구는 하절기에 지속적으로 발생하는 고수온, 적조와 같은 피해를 최소화하기 위한 방안으로 해상가두리 ($6m{\times}6m{\times}6m$) 절식 사육을 통해 참돔의 생존율, 성장, 성장 회복 그리고 생리반응을 조사하였다. 6주간의 절식 후 4주간의 먹이 공급을 통한 연구결과 생존율은 섭식구 94.4%, 절식구 98.1%로 나타났다. 체중 성장률은 절식구에서 $-12.4{\pm}3.2%$ 성장을 보여 체중의 유의한 감소를 보였지만, 먹이 공급 후 $36.7{\pm}6.8%$의 빠른 성장 회복을 나타냈다. 혈액분석을 통한 절식구의 영양상태 또한 섭식구에 비해 유의하게 감소하였지만 먹이 공급 후 개시 시 수준으로 빠르게 회복하였다. 또한 스트레스 지표를 분석한 결과 절식기간 섭식구의 Hb, Ht의 수치가 증가하였으며, 섭식구의 급격한 혈액성상의 변화는 하절기 해상가두리 환경에서 절식구보다 섭식구가 스트레스에 더 민감한 것으로 나타났다. 따라서 본 연구의 생존율, 성장 회복, 혈액성상을 통한 결과를 토대로, 고수온과 적조에 노출되는 하절기에 피해를 최소화하기 위한 가두리 양식어류 관리 방안으로 일정기간 절식이 활용 가능할 것으로 여겨진다.

시판 생막걸리에서 분리한 유산균의 프로바이오틱스 기능성 연구 (Probiotic Properties of Lactic Acid Bacteria Isolated from Commercial Raw Makgeolli)

  • 정상은;김세헌
    • 한국식품과학회지
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    • 제47권1호
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    • pp.44-50
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    • 2015
  • 본 연구에서는 시판 생막걸리에서 분리한 유산균의 probiotics 특성을 연구하기 위해 내산성, 내담즙성, 장 부착능에 효능이 있는 6개 균주를 선별하여 동정하였다. 생막걸리에서 분리한 유산균을 pH 2.5의 산성 및 담즙산(0.3% oxgall) 조건에서 내산성과 내담즙성을 측정한 결과 BSM-2, BSM-3, GSM-3, TJH-1, EHJ-1, EHJ-2의 6개 균주에서 각각 $10^7$, $10^8CFU/mL$ 높은 생존률을 나타내어 우수한 내산성과 내담즙성을 확인할 수 있었다. 내산성과 내담즙성에 효능이 있는 6개 균주의 16S rDNA 분석 결과, L. plantarum (BSM-2, EHJ-1) L. casei (GSM-3, EHJ-2), L. brevis (BSM-3), P. pentosaceus (TJH-1)의 4개 group이 동정되었다. 장부착능에서는 다른 유산균에 비해 장내 부착능이 뛰어난 것으로 알려진 L. rhamnosus GG를 대조군으로 사용하였으며 HT-29 cell에 대해 6종의 유산균이 모두 장 부착능을 보였으며 L. casei(GSM-3, EHJ-2)가 가장 장 부착능이 높았다. 항산화능력은 L. plantarum BSM-2와 EHJ-1에서 각각 32.44%, 24.65%의 DPPH radical 소거능을 보였다. 시판 생막걸리에서 분리된 유산균은 다양한 식중독 원인 세균에 대하여 항균활성을 지니고 있었으며, 본 연구에서는 유산균이 생성하는 항균물질인 bacteriocin이 아닌 유산균 발효 중 젖산을 생성하면서 pH의 저하로 항균활성을 보인다는 결과를 확인할 수 있었다. Probiotics로의 기능성을 가진 6개 균주의 콜레스테롤 저하능력은 L. plantarum BSM-2와 EHJ-1에서 각각 68.82%, 56.38%의 높은 감소율을 나타내었다. 시판 생막걸리에서 분리한 유산균의 probiotic 특성을 조사해 본 결과 L. plantarum BSM-2, EHJ-1, L. brevis BSM-3, L. casei GSM-3, EHJ-2, P. pentosaceus TJH-1 모두 프로바이오틱스의 기본특성을 지니고 있어 프로바이오틱 균주로서 이용가치가 충분하다고 판단되어지며, L. plantarum BSM-2가 내산성, 내담즙성이 가장 우수한 결과를 나타내었고, 장 부착성이 우수하였으며, 뛰어난 항산화 능력과 항균력을 지니며, 콜레스테롤 저하능도 뛰어나 가장 우수한 프로바이오틱 균주라고 판단된다.

C. difficile 톡신이 야기하는 대장상피세포 미세소관 변형에 대한 초산의 억제 효능 (Acetic Acid Recovers Microtubule Disassembly Caused by Clostridium difficile Toxin A in Human Colonocytes through Increased Tubulin Acetylation)

  • 윤이나;김호
    • 생명과학회지
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    • 제28권8호
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    • pp.885-891
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    • 2018
  • 급성위막성대장염(Pseudomembranous colitis)은 C. difficile 세균이 분비하는 톡신A에 의해 유발되는 것으로 알려져 있다. 톡신A에 의한 점막 상피세포의 장벽기능 감소가 발병 원인으로 알려져 있다. 최근 연구에 의하면 톡신 A는 대장상피세포 속 HDAC-6의 활성을 높여 튜블린의 탈아세틸화를 증가시키는 것으로 알려져 있다. 튜블린 단백질의 탈아세틸화는 미세소관 불 형성을 초래하여 점막 상피세포의 극단적인 세포 형태 변형을 야기하게 되며 결국 상피세포의 고유기능인 장벽 기능이 파괴된다고 알려져 있다. 최근 연구자 등은 potassium acetate가 톡신A에 의한 튜블린 탈아세틸화와 미세소관 불 형성을 회복시켜 장염을 유의하게 억제함을 보고하였다. 따라서 본 연구에서는 아세틸기를 포함하는 또 다른 간단한 화학구조의 초산을 적용하여 톡신A의 세포독성을 억제하는지 확인해보고자 하였다. 인간 대장상피세포에서 초산 자극은 튜블린 단백질의 아세틸화를 유의하게 증가시켰다. 또한 초산은 대장상피세포 속 미세소관 형성과정도 강하게 촉진시킴을 확인하였다. 초산은 톡신A에 의한 튜블린 탈아세틸화와 미세소관 불 형성 그리고 세포독성 모두를 유의하게 회복시켰다. 이상의 결과는 초산에 의한 미세소관 형성 촉진이 톡신A에 의해 초래되는 세포골격계 파괴와 그로 인한 세포독성을 억제할 수 있음을 보여준다. 따라서 초산이 톡신A의 작용을 차단하여 위막성대장염 증상을 완화시킬 수 있는 치료제로서 개발 가치가 있음을 보여준다.

팽이버섯 에탄올 추출물의 생리활성 (Physiological activity of Flammulina velutipes sp. ethanol extract)

  • 조세현;진경언;우양;최재선;윤형식;유영복;박기문
    • 한국버섯학회지
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    • 제8권4호
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    • pp.150-156
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    • 2010
  • 팽이버섯 11계통을 사용하여 polyphenol 및 ${\beta}$-glucan 함량을 분석하고, 생리활성으로 항산화 및 항암, 항고혈압, 항당뇨, 항염활성을 측정하였다. Polyphenol 함량에서는 CBMFV-65가 244.74 mg%로 가장 많은 함량을 나타내었고 전 품종에서 전반적으로 100 mg% 이상의 함량을 나타냈다. ${\beta}$-glucan 함량에서는 CBMFV-41에서 27.37%로 가장 높았으며, 그 다음으로 CBMFV-30 27.21%, CBMFV-65 27.11%의 순서로 높은 ${\beta}$-glucan 함량을 나타냈다. 전자공여능에서는 CBMFV-66이 91.74%로 가장 높은 DPPH 소거활성을 나타냈으며 전체적으로 70%의 높은 소거활성을 보였다. 세포독성 실험에서는 폐암세포에 대한 세포 저해활성이 가장 컸으며, 폐암세포와 대장암세포 모두 CBMFV-30에서 각각 76.07%, 67.05%로 가장 높은 세포 저해활성을 나타냈다. ACE 저해활성에서는 CBMFV-65에서 10.96%를 나타냈고 나머지 품종은 10%미만의 저해활성을 나타냈다. 항당뇨 활성에서는 CBMFV-41에서 63.57%의 효소 저해율이 측정되었고, CBMFV-63은 10.98%로 가장 낮은 효소 저해활성을 나타냈다. 마지막으로 항염활성에서는 CBMFV-41에서 61.44%의 nitric oxide 억제율이 측정되었다.

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Novel SIRT Inhibitor, MHY2256, Induces Cell Cycle Arrest, Apoptosis, and Autophagic Cell Death in HCT116 Human Colorectal Cancer Cells

  • Kim, Min Jeong;Kang, Young Jung;Sung, Bokyung;Jang, Jung Yoon;Ahn, Yu Ra;Oh, Hye Jin;Choi, Heejeong;Choi, Inkyu;Im, Eunok;Moon, Hyung Ryong;Chung, Hae Young;Kim, Nam Deuk
    • Biomolecules & Therapeutics
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    • 제28권6호
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    • pp.561-568
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    • 2020
  • We examined the anticancer effects of a novel sirtuin inhibitor, MHY2256, on HCT116 human colorectal cancer cells to investigate its underlying molecular mechanisms. MHY2256 significantly suppressed the activity of sirtuin 1 and expression levels of sirtuin 1/2 and stimulated acetylation of forkhead box O1, which is a target protein of sirtuin 1. Treatment with MHY2256 inhibited the growth of the HCT116 (TP53 wild-type), HT-29 (TP53 mutant), and DLD-1 (TP53 mutant) human colorectal cancer cell lines. In addition, MHY2256 induced G0/G1 phase arrest of the cell cycle progression, which was accompanied by the reduction of cyclin D1 and cyclin E and the decrease of cyclin-dependent kinase 2, cyclin-dependent kinase 4, cyclin-dependent kinase 6, phosphorylated retinoblastoma protein, and E2F transcription factor 1. Apoptosis induction was shown by DNA fragmentation and increase in late apoptosis, which were detected using flow cytometric analysis. MHY2256 downregulated expression levels of procaspase-8, -9, and -3 and led to subsequent poly(ADP-ribose) polymerase cleavage. MHY2256-induced apoptosis was involved in the activation of caspase-8, -9, and -3 and was prevented by pretreatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, the autophagic effects of MHY2256 were observed as cytoplasmic vacuolation, green fluorescent protein-light-chain 3 punctate dots, accumulation of acidic vesicular organelles, and upregulated expression level of light-chain 3-II. Taken together, these results suggest that MHY2256 could be a potential novel sirtuin inhibitor for the chemoprevention or treatment of colorectal cancer or both.

Antispastic Effect of Electroacupuncture on Upper Extremity in Stroke Patients by T-reflex Study : A Single-Blind, Randomized Controlled, Preliminary Study

  • Cho, Min Kyoung;Lee, In;Kwon, Jung Nam;Shin, Byung Cheul;Ko, Sung Hwa;Ko, Hyun Yoon;Shin, Yong Il;Hong, Jin Woo
    • 대한한의학회지
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    • 제36권4호
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    • pp.8-18
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    • 2015
  • Objectives: There have been several studies evaluated effect of electroacupuncture (EA) on spasticity but most studies could not assess spasticity quantitatively because they used clinical rating scales for assessment spasticity. The objective of this study is to evaluate effect of EA on poststroke spasticity quantitatively using tendon reflex (T-reflex). Methods: 29 stroke patients with upper extremity spasticity were randomized to EA group and control group. The EA group received combined EA and rehabilitation therapy 5 times a week for 3 weeks. Acupuncture treatment was given at Jian Yu (LI 15), Qu Chi (LI 11), Shao Hai (HT 3), Wai Guan (TE 5), He Gu (LI 4), Lie Que (LU 7), Hou Xi (SI 3) of the affected side, 30 minutes of electrical stimulation with a frequency of 40/13 Hz was applied at Qu Chi (LI 11), He Gu (LI 4). The control group received only rehabilitation therapy. The efficacy of treatment was assessed using T-reflex latency and amplitude, modified Ashworth scale (MAS) of biceps brachii, brachioradialis and triceps brachii. Fugl-Meyer motor function assessment (FMA) and functional independence measure (FIM) were also measured to assess motor function and functional independence. All outcomes were measured before treatment, immediately after 3 weeks of treatment and 1 week after 3 weeks of treatment. Results: No statistically significant differences were found in outcomes including T-reflex between the study groups except for FIM values immediately after 3 weeks of treatment (p=0.037). Conclusions: These results suggest that 3 weeks of EA does not reduce poststroke upper extremity spasticity electrophysiologically and clinically. However, small sample sizes and contradictory tendency between results from T-reflex and those from MAS require cautious judgement on interpretation of the results. A larger, well-designed clinical trials for quantitative evaluation of effect of EA on poststroke spasticity will be needed.

Anti-carcinogenic effects of non-polar components containing licochalcone A in roasted licorice root

  • Park, So Young;Kim, Eun Ji;Choi, Hyun Ju;Seon, Mi Ra;Lim, Soon Sung;Kang, Young-Hee;Choi, Myung-Sook;Lee, Ki Won;Yoon Park, Jung Han
    • Nutrition Research and Practice
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    • 제8권3호
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    • pp.257-266
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    • 2014
  • BACKGROUND/OBJECTIVE: Licorice has been shown to possess cancer chemopreventive effects. However, glycyrrhizin, a major component in licorice, was found to interfere with steroid metabolism and cause edema and hypertension. The roasting process of licorice modifies the chemical composition and converts glycyrrhizin to glycyrrhetinic acid. The purpose of this study was to examine the anti-carcinogenic effects of the ethanol extract of roasted licorice (EERL) and to identify the active compound in EERL. MATERIALS/METHODS: Ethanol and aqueous extracts of roasted and un-roasted licorice were prepared. The active fraction was separated from the methylene chloride (MC)-soluble fraction of EERL and the structure of the purified compound was determined by nuclear magnetic resonance spectroscopy. The anti-carcinogenic effects of licorice extracts and licochalcone A was evaluated using a MTT assay, Western blot, flow cytometry, and two-stage skin carcinogenesis model. RESULTS: EERL was determined to be more potent and efficacious than the ethanol extract of un-roasted licorice in inhibiting the growth of DU145 and MLL prostate cancer cells, as well as HT-29 colon cancer cells. The aqueous extracts of un-roasted and roasted licorice showed minimal effects on cell growth. EERL potently inhibited growth of MCF-7 and MDA-MB-231 breast, B16-F10 melanoma, and A375 and A2058 skin cancer cells, whereas EERL slightly stimulated the growth of normal IEC-6 intestinal epithelial cells and CCD118SK fibroblasts. The MC-soluble fraction was more efficacious than EERL in inhibiting DU145 cell growth. Licochalcone A was isolated from the MC fraction and identified as the active compound of EERL. Both EERL and licochalcone A induced apoptosis of DU145 cells. EERL potently inhibited chemically-induced skin papilloma formation in mice. CONCLUSIONS: Non-polar compounds in EERL exert potent anti-carcinogenic effects, and that roasted rather than un-roasted licorice should be favored as a cancer preventive agent, whether being used as an additive to food or medicine preparations.

Anticancer and Immunopotentiating Activities of Crude Polysaccharides from Pleurotus nebrodensis on Mouse Sarcoma 180

  • Cha, Youn Jeong;Alam, Nuhu;Lee, Jae Seong;Lee, Kyung Rim;Shim, Mi Ja;Lee, Min Woong;Kim, Hye Young;Shin, Pyung Gyun;Cheong, Jong Chun;Yoo, Young Bok;Lee, Tae Soo
    • Mycobiology
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    • 제40권4호
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    • pp.236-243
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    • 2012
  • Pleurotus nebrodensis is an edible and commercially available mushroom in Korea. This study was conducted in order to evaluate the anticancer and immunopotentiating activities of crude polysaccharides, extracted in methanol, neutral saline, and hot water (hereafter referred to as Fr. MeOH, Fr. NaCl, and Fr. HW, respectively) from the fruiting bodies of P. nebrodensis. ${\beta}$-Glucan and protein contents in Fr. MeOH, Fr. NaCl, and Fr. HW extracts of P. nebrodensis ranged from 23.79~36.63 g/100 g and 4.45~6.12 g/100 g, respectively. Crude polysaccharides were not cytotoxic against sarcoma 180, HT-29, NIH3T3, and RAW 264.7 cell lines at a range of $10{\sim}2,000{\mu}g/mL$. Intraperitoneal injection with crude polysaccharides resulted in a life prolongation effect of 11.76~27.06% in mice previously inoculated with sarcoma 180. Treatment with Fr. NaCl resulted in an increase in the numbers of spleen cells by 1.49 fold at the concentration of $50{\mu}g/mL$, compared with control. Fr. HW improved the immuno-potentiating activity of B lymphocytes through an increase in alkaline phosphatase activity by 1.65 fold, compared with control at $200{\mu}g/mL$. Maximum production of nitric oxide ($14.3{\mu}M$) was recorded in the Fr. NaCl fraction at $200{\mu}g/mL$. Production of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), and interleukin-6 (IL-6) was significantly higher, compared to control, and IL-6 production was highest, in contrast to TNF-${\alpha}$, IL-$1{\beta}$, and positive control, concanavalin at the tested concentration of the various fractions. Results of the current study suggest that polysaccharides extracted from P. nebrodensis have a strong anticancer effect and may be useful as an ingredient of biopharmaceutical products for treatment of cancer.