• Journal of Genetic Medicine
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• v.1 no.1
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• pp.45-50
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• 1997

Neuroblastoma, a pediatric malignant neoplasm of neural crest origin, has a wide range of clinical virulence. The mechanisms contributing to the development of neuroblastomas are largely unclear, but non-random chromosomal changes identified over the past years suggest the involvement of genetic alterations. Amplification of the human N-myc proto-oncogene is frequently seen either in extrachromosomal double minutes or in homogeneously staining regions (HSRs) of aggressively growing neuroblastomas. N-myc maps to chromosome 2 band 24, but HSR have never been observed at this band, suggesting transposition of N-myc during amplification. We have constructed and analyzed the region-specific painting probe for HSR in neuroblastoma IMR-32 to determine the derivative chromosomes. Microdissection was performed on HSR using an inverted microscope with the help of microglass needles and an micromanipulator. We pretreated the microdissected fragments with Topoisomerase I which catalyzes the relaxation of supercolled DNA, and performed two initial rounds of DNA synthesis with T7 DNA polymerase followed by conventional PCR to enable the reliable preparation of Fluorescent in situ hybridization probe from a single microdissected chromosome. With this method, it was possible to construct the region-specific painting probe for HSR. The probe hybridized specifically to the HSRs of IMR-32, and to 2p24, 2p13 of normal chromosome. Our results suggest there was coamplification of N-myc together with DNA of the chromosome 2p24 and 2p13. Moreover, the fluorescent signals for the amplified chromosomal regions in IMR-32 cells were also easily recognized at a Thus this painting probe can be applied to detect the similar amplification of N-myc in neuroblastoma tissue, and the probe pool for HSR may be used to identify the cancer-relevant genes.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.20 no.3
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• pp.1-19
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• 2021

The domastic hard shoulder running(HSR) System has been gradually expanding since its initial implementation in September 2007 with the aim of increasing capacity and resolving congestion. Hard Shoulder is used as a space for driver's visual comfort and a place for vehicles to evacuate in case of emergency, but it is replaced by a space for driving when the HSR System is implemented. Therefore, it was intended to determine the improvement effect before and after implementation of the HSR system through safety analysis and mobility analysis. The safety analysis analyzed the impact of traffic accidents by comparing HSR sections and similar sections. The mobility analysis was to determine the improvement effect by quantifying the speed and traffic volume changes before and after HSR System implementation. According to safety yanalysis, there is no effect of reducing traffic accidents when implementing the HSR System. In mobility analysis, the implementation of the HSR System significantly improved the speed of traffic during peak hours and significantly reduces slow and delay hours.

• Journal of Sensor Science and Technology
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• v.14 no.3
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• pp.144-149
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• 2005

In this paper, we introduce the variation of ride characteristics for HSR 350x(Korea High Speed Train project) through on-line test during about 3 years. The concept of term "ride comfort" is equivocal. Generally it is evaluated as the vehicle vibration. The ride comfort for HSR 350x has been assessed by statistical method according to UIC 513R. The testing results show that HSR 350x has no problems from the viewpoint of the comfort ride on the high speed line and conventional line and that 1st and 2nd suspensions play an important role in the ride characteristics for high speed train.

• Journal of Korea Water Resources Association
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• v.54 no.1
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• pp.27-38
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• 2021

Recently, many studies have been conducted to use the radar rainfall in hydrology. However, in the case of weather radar, the beam is blocked due to the limitation of the observation such as mountain effect, which causes underestimation of the radar rainfall. In this study, the radar rainfall was estimated using the Hybrid Sacn Reflectivity (HSR) technique for hydrological use of weather radar and the runoff analysis was performed using the GRM model which is a distributed rainfall-runoff model. As a result of performing the radar rainfall correction and runoff simulation for 5 rainfall events, the accuracy of the dual-polarization radar rainfall using the HSR technique (Q_H_KDP) was the highest with an error within 15% of the ground rainfall. In addition, the result of runoff simulation using Q_H_KDP also showed an accuracy of R2 of 0.9 or more, NRMSE of 1.5 or less and NSE of 0.5 or more. From this study, we examined the application of the dual-polarization radar and this results can be useful for studies related to the hydrological application of dual-polarization radar rainfall in the future.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.229-233
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• 2002

Ferritin is ubiquitous in bacteria, animals and plants. Ferritin is thought to play two main roles in living cells to provide iron for the synthesis of iron protein such as ferretoxin and cytochromes and to prevent damage from radicals produced by iron/dioxygen interaction. To enhance the resistance of potato to Erwinia carotovora, the soybean ferritin gene was introduced into the potato either under CaMV 35S or hsr203J promoter. Potato transgenic plants were screened by PCR analysis using specific primers to the ferritin gene. Expression of ferritin gene under CaMV 35S and hsr203J promoter in potato transgenic plants was confirmed by northern blot analysis. hsr203J promoter known to pathogen inducible in tobacco drives the induction upon Phytophthora infestan in potato and the transcript level of ferritin gene was extremely high after 24 hours post inoculation. One of transformants under CaMV 35S promoter was increased 2.5 fold than untransformant. Each one of transgenic potato containing gene promoter CaMV 35S and hsr203J-ferrtin fusion exhibited tolerance against potato soft rot.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.12
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• pp.1735-1740
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• 2005

Forty-two single-bearing and 42 twin-bearing mature Angus${\times}$Hereford cows were allocated, seven per cell to 3 replications of 2 stocking rates (3.2 cows/ha; medium stocking rate [MSR], and 3.8 cows/ha; high stocking rate [HSR]) to graze summer-active and winter-active pastures from late pregnancy to the weaning of their calves. Cow liveweights and growth of calves were recorded as well as estimates of pasture quantity and forage intake. Pasture quantity did not differ in the paddocks grazed by single- and twin-bearing cows during pregnancy, nor effectively did forage intake. Subsequently, intake was higher during mid-lactation especially with twin-rearing cows (25% higher than single-rearing cows at the MSR; 9% at the HSR). However, quantity of pasture decreased for twin-rearing cows and was less than that available to single-rearing cows as lactation progressed. Liveweights of twinrearing cows decreased by 16% from late pregnancy to weaning at the MSR, and by 14% at the HSR, compared to decreases of 1% for single-rearing cows. Twin calves were lighter at birth, had slower growth rates, and were lighter at weaning than single calves. In spite of weaning smaller calves twinning increased the output (kg of calf weaned) per cow and per ha, and increased the efficiency (kg calf weaned per unit of forage eaten by the cow) over single calf production by 46% at the MSR and by 58% at the HSR. Twinning also increased the marginal returns from investment in high input pastures required by the enterprise.

• Journal of the Korean Society for Railway
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• v.7 no.4
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• pp.332-338
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• 2004

The two projects about the high speed train have been performed in Korea, one project is the commercial run of high speed train(KTX) from Seoul to Busan and the other is the development of original high speed train(HSR 350x). As the successful results, the service run of KTX had been launched on the 1st of April, 2004 and HSR 350x had been fabricated in June, 2002 and has been tested in the Kyoungbu line and Honam line since then. The railway has the track irregularities which cause vibrations, such as rail joints, turnout, level crossing, transition curves and super-elevation ramps, and variations in the track level(z-axis) and the gauge(y-axis). Generally, the ride comfort for railway is evaluated by using the vibrations. In this paper, the ride comfort indices according to mc 513R and ISO 10056 have been reviewed when the high-speed trains are operated on both Kyoungbu line and Honam line. Also, the ride characteristics for KTX and HSR 350x on the high speed line and the conventional line have been analyzed and evaluated. The results show that the high-speed train has no problem from the viewpoint of the comfort ride on the high speed line and the conventional line and the ride index for HSR 350x is same as that for KTX in the high speed(300 km/h).

• Korean Journal of Remote Sensing
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• v.31 no.4
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• pp.321-330
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• 2015

Atmospheric correction is an essential part in time-series analysis on biophysical parameters of surface features. In this study, we tried to examine possible problems in atmospheric correction of multitemporal High Spatial Resolution (HSR) images obtained from two different sensor systems. Three KOMPSAT-2 and two IKONOS-2 multispectral images were used. Three atmospheric correction methods were applied to derive surface reflectance: (1) Radiative Transfer (RT) - based absolute atmospheric correction method, (2) the Dark Object Subtraction (DOS) method, and (3) the Cosine Of the Uun zeniTh angle (COST) method. Atmospheric correction results were evaluated by comparing spectral reflectance values extracted from invariant targets and vegetation cover types. In overall, multi-temporal reflectance from five images obtained from January to December did not show consistent pattern in invariant targets and did not follow a typical profile of vegetation growth in forests and rice field. The multi-temporal reflectance values were different by sensor type and atmospheric correction methods. The inconsistent atmospheric correction results from these multi-temporal HSR images may be explained by several factors including unstable radiometric calibration coefficients for each sensor and wide range of sun and sensor geometry with the off-nadir viewing HSR images.

• Bulletin of the Korean Chemical Society
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• v.17 no.8
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• pp.730-735
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• 1996

The layered perovskite oxide, KSr2Nb3O10, was synthesized. The interlayer potassium cations were readily exchanged by protons in hydrochloric acid solution to give the protonation compound, HSr2Nb3O10·0.5H2O. The intercalation compounds, [NH3(CH2)nNH3]xSr2Nb3O10, were also obtained by acid-base reactions between the protonation compound and organic bases, 1,n-alkyldiamines. The interlayer distances in the intercalation compounds were linearly increased with the increase of the number of carbon (Δc/Δn=1.05 Å) in 1,n-alkyldiamines. The intercalated alkyldiammonium ions formed a paraffin-like monolayer with average tilting angle (θ) of ca. 56°. The intercalation reactions occurred stoichiometrically. The thermal decomposition process of the intercalation compounds showed distinct three steps due to the desorption of hydrated water, the decomposition of organic moiety, and the decomposition of Sr-related compounds.

• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.205-211
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• 2021

In order to test the functionality of Panax ginseng thioredoxin 1 (PgTRX1) isolated from fermented wild ginseng roots, a transient effect on physiological activity were performed over a short time frame using the Agrobacterium infiltration technique. The PgTRX1 gene isolated from fermented wild ginseng was confirmed to have a size of 579 bp, and the expression of PgTRX1 was the highest in the sample after 6 h of fermentation. As a result of constructing this gene and confirming the infiltration reaction mediated by Agrobacterium in tobacco leaves, it was found that the expression of the NbHSR203j gene was also induced as PgTRX1 expression increased. As a result of measuring the biological activity of the infiltration samples, the total phenol content increased by 35.45±1.84 to 49.01±1.84 ㎍ GAE/mL compared to the control, and the total flavonoid amount of 9.52±0.41 to 9.82±0.25 ㎍ QE/mL was slightly high. From these results, Agrobacterium-mediated PgTRX1 appears to be related to the hypersensitive response induction mechanism of plants and the production of secondary metabolites such as phenolic substances.