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http://dx.doi.org/10.3839/jabc.2021.029

Molecular characterization and biological changes caused by Agrobacterium-mediated infiltration of PgTRX1  

Choi, Seung Hyuk (Department of Bio-Resource Sciences, Kangwon National University)
Seo, Ji Won (Interdisciplinary Program in Smart Science, Kangwon National University)
Lee, Jae Geun (Research Institute of Biotechnology, HwajinBioCosmetic)
Yu, Chang Yeon (Interdisciplinary Program in Smart Science, Kangwon National University)
Seong, Eun Soo (Department of Medicinal Plant, Suwon Women's University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.3, 2021 , pp. 205-211 More about this Journal
Abstract
In order to test the functionality of Panax ginseng thioredoxin 1 (PgTRX1) isolated from fermented wild ginseng roots, a transient effect on physiological activity were performed over a short time frame using the Agrobacterium infiltration technique. The PgTRX1 gene isolated from fermented wild ginseng was confirmed to have a size of 579 bp, and the expression of PgTRX1 was the highest in the sample after 6 h of fermentation. As a result of constructing this gene and confirming the infiltration reaction mediated by Agrobacterium in tobacco leaves, it was found that the expression of the NbHSR203j gene was also induced as PgTRX1 expression increased. As a result of measuring the biological activity of the infiltration samples, the total phenol content increased by 35.45±1.84 to 49.01±1.84 ㎍ GAE/mL compared to the control, and the total flavonoid amount of 9.52±0.41 to 9.82±0.25 ㎍ QE/mL was slightly high. From these results, Agrobacterium-mediated PgTRX1 appears to be related to the hypersensitive response induction mechanism of plants and the production of secondary metabolites such as phenolic substances.
Keywords
Agrobacterium infiltration technique; NbHSR203j; Panax ginseng thioredoxin 1; Total phenol content; Total flavonoid amount;
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